NAD/NADH比率检测试剂盒,比色法荧光法简单快速!

NADH,烟酰胺腺嘌呤二核苷酸,还原态,还原型辅酶Ⅰ。N指烟酰胺,A指腺嘌呤,D是二核苷酸。葡萄糖代谢时直接经代谢所产生的ATP是十分的少的,而代谢产生的NADH或FADH2经由一个电子传递与氧化磷酸反应可产生大量的ATP。NADH分子是线粒体中能量产生链中的控制标志物。NADH水平的上升指示代谢失衡的出现。
NAD/NADH

 

监视NAD/NADH的氧化还原状态是表征活体内线粒体功能的最佳参数。紫外光可以在线粒体中激发NADH产生荧光,用来监测线粒体功能。一般来说,涉及到氧化还原的反应都用得到,比如呼吸作用,光合作用,等等氨会抑制呼吸过程中的电子传递系统,尤其是NADH。因此NAD/NADH水平的监控是能量传递、细胞核组织氧化还原态等研究的主要兴趣点。

 

有没有一款可以同时检测,NAD+,NADH,以及NAD+/NADH比率,这三个指标的试剂盒呢?

 

艾美捷科技特向您推荐,已被广泛使用,发布文章不计其数的高品质NAD/NADH比率检测试剂盒。

 

货号 产品名称 规格
15273 Amplite™ Colorimetric NAD/NADH Ratio Assay Kit 比色法
15263 Amplite™ Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence* 荧光法

*样品通过NAD萃取,同时检测(NAD+NADH)与(NAD)的结果,相减计算得出NADH含量,以及NAD/NADH的比率。

 

NAD/NADH 比率试剂盒

 

原理:Amplite 比色法NAD/NADH比率检测试剂盒,提供一个比色法原理的检测培养细胞的总NAD/NADH,和NAD/NADH ratio的方法。在这个实验中,裂解液中NAD可以被NAD萃取液提取,通过酶反应被转化为NADH,然后利用NADH探针反应,产生黄色染料,此染料可以在460nm处测吸收峰,或者加入增强剂后,可以检测635nm吸收峰。染料产生颜色与细胞裂解液中NAD 或者NADH的浓度呈正比。

 

NAD/NADH 比率试剂盒

《卓越的标准曲线》

 

NAD/NADH 比率试剂盒

《发表文章结果:Ren, T., et al. Oncogene  36.42(2017).》

 

 

 引用文献 

  • Norisoboldine, a natural AhR agonist, promotes Treg differentiation and attenuates colitis via targeting glycolysis and subsequent NAD+/SIRT1/SUV39H1/H3K9me3 signaling pathway

Authors: Qi Lv, Kai Wang, Simiao Qiao, Ling Yang, Yirong Xin, Yue Dai, Zhifeng Wei

Journal: Cell death & disease (2018): 258

 

  • Stochastic expression of lactate dehydrogenase A induces Escherichia coli persister formation

Authors: Naoki Yamamoto, Rino Isshiki, Yuto Kawai, Daiki Tanaka, Tetsushi Sekiguchi, Shinya Matsumoto, Satoshi Tsuneda

Journal: Journal of Bioscience and Bioengineering (2018)

 

  • Celastrol attenuates angiotensin II mediated human umbilical vein endothelial cells damage through activation of Nrf2/ERK1/2/Nox2 signal pathway

Authors: Miao Li, Xin Liu, Yongpeng He, Qingyin Zheng, Min Wang, Yu Wu, Yuanpeng Zhang, Chaoyun Wang

Journal: European Journal of Pharmacology (2017): 124--133

 

  • Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation

Authors: Fei Yang, Caitlin Heit, Debra L Inglis

Journal: Fermentation (2017): 61

 

  • Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase

Authors: Min Ling, Peixin Huang, Shamima Islam, Daniel P Heruth, Xuanan Li, Li Qin Zhang, Ding-You Li, Zhaohui Hu, Shui Qing Ye

Journal: Cell & Bioscience (2017): 27

 

  • MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells

Authors: T Ren, H Zhang, J Wang, J Zhu, M Jin, Y Wu, X Guo, L Ji, Q Huang, H Yang

Journal: Oncogene (2017)

 

  • Metabolic and molecular insights into an essential role of nicotinamide phosphoribosyltransferase

Authors: Li Q Zhang, Leon Van Haandel, Min Xiong, Peixin Huang, Daniel P Heruth, Charlie Bi, Roger Gaedigk, Xun Jiang, Ding-You Li, Gerald Wyckoff

Journal: Cell Death & Disease (2017): e2705

 

  • Pyrroloquinoline Quinone, a Redox-active o-Quinone, Stimulates Mitochondrial Biogenesis by Activating SIRT1/PGC-1α Signaling Pathway

Authors: Kazuhiro Saihara, Ryosuke Kamikubo, Kazuto Ikemoto, Koji Uchida, Mitsugu Akagawa

Journal: Biochemistry (2017)

 

  • Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio

Authors: Gang Luo, Bingqing Huang, Xiang Qiu, Lin Xiao, Ning Wang, Qin Gao, Wei Yang, Liping Hao

Journal: Molecular Nutrition & Food Research (2017)

 

  • A Snapshot of the Plant Glycated Proteome STRUCTURAL, FUNCTIONAL, AND MECHANISTIC ASPECTS

Authors: Tatiana Bilova, Elena Lukasheva, Dominic Brauch, Uta Greifenhagen, Gagan Paudel, Elena Tarakhovskaya, Nadezhda Frolova, Juliane Mittasch, Gerd Ulrich Balcke, Alain Tissier

Journal: Journal of Biological Chemistry (2016): 7621--7636

 

  • AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD+ elevation

Authors: Xiaojuan Han, Haoran Tai, Xiaobo Wang, Zhe Wang, Jiao Zhou, Xiawei Wei, Yi Ding, Hui Gong, Chunfen Mo, Jie Zhang

Journal: Aging cell (2016): 416—427

 

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