β-羟丁酸(β-HB)定量分析试剂盒,你的首选!

摘要

β-羟丁酸试剂盒可检测各种生物样品内的β-羟丁酸含量,快速、灵敏、准确!你的首选!

丙酮、乙酰乙酸和β-羟丁酸(β-Hydroxybutyrate, β-HB)统称为酮体(ketone body),当胰岛素水平下降到非常低的水平时,发生糖尿病酮症酸中毒,关闭葡萄糖供给身体。生理反应是由脂肪酸氧化产生的乙酰辅酶A产生酮体(主要是β-羟基丁酸酯,占75%以上)。酮体生产率非常高,超过了身体利用它们作为能量来源的能力,血液浓度越来越高,称酮血症(ketonemia)。过多的酮体从尿中排出,称酮尿(Ketonuria)。因酮体中除丙酮外,均为强酸物质,过多的酮体由尿排出时,必须与体内碱储(主要是Na+)结合成盐类,因此体内碱储消耗过多,最后导致代谢性酸中毒及脱水,这种病理变化称为酮症(Ketosis)。

 

β-Hydroxybutyrate-1

 

如何检测样品中β-羟丁酸含量的试剂盒

艾美捷科技为您推荐享誉全球市场的β-羟丁酸(β-HB)定量分析试剂盒: K632-100

 

原理:β-羟丁酸(β-HB)检测试剂盒利用β-羟丁酸脱氢酶参与反应,将样本内的β-羟丁酸催化脱氢,其产物能与发光探针反应产生450nm处的吸收峰,吸光值与其含量成正比,本试剂盒可检测各种生物样品。

 

CBP Tagtel
产品名称及描述 货号 产品说明
β-Hydroxybutyrate (β-HB) Colorimetric Assay Kit K632-100 详情

*β-羟丁酸试剂盒启用后,按照要求条件保存各个组分,并在2个月内用完。

 

β-羟丁酸试剂盒特点:

  1. 步骤简单,只需40分钟
  2. 检测范围:01-0.2 mM
  3. 样品类型:血清,血浆,细胞裂解液,尿液

 

Tips:

  1. 推荐使用肝素(Heparin)收集血液
  2. 对于样品可能存在的检测干扰物,建议使用10kDa的超滤管,过滤,收集澄清的样品
  3. 血清样品可直接检测,其它样品需要做梯度稀释,确保检测结果在标准曲线范围内

 β-HB-standard-curve

《卓越的标准曲线》

 

引用文献

Anja Solberg et al., Deletion of mouse Alkbh7 leads to obesity. J Mol Cell Biol, Jun 2013; 5: 194 - 203.Blouet, C. et. al. Nutrient-Sensing Hypothalamic TXNIP Links Nutrient Excess to Energy Imbalance in Mice. J. Neurosci., Apr 2011; 31: 6019 - 6027.

Fabbrini E. et.al. Effect of Fenofibrate and Niacin on Intrahepatic Triglyceride Content, Very Low-Density Lipoproteins Kinetics, and Insulin Action in Obese Subjects with Nonalcoholic Fatty Liver Disease J. Clin. Endocrinol. Metab., Apr 2010; 10.1210/jc.2009-2622.

Garg, Manisha et al. (2017) Liraglutide acutely suppresses glucagon, lipolysis and ketogenesis in type 1 diabetes, Diabetes Obes Metab. 2017 Mar 17.

Goldgof et al., The Chemical Uncoupler 2,4-Dinitrophenol (DNP) Protects against Diet-induced Obesity and Improves Energy Homeostasis in Mice at Thermoneutrality. J. Biol. Chem., Jul 2014; 289: 19341 - 19350.

Huijsman E et al (2009) Am J Physiol Endocrinol Metab; 10.1152/ajpendo.00190.2009.

Janna A. van Diepen et al., Aspirin reduces hypertriglyceridemia by lowering VLDL-triglyceride production in mice fed a high-fat diet, Am J Physiol Endocrinol Metab, Dec 2011; 301: E1099 - E1107.

Ji et al., Molecular and metabolic profiles suggest that increased lipid catabolism in adipose tissue contributes to leanness in domestic chickens.Physiol Genomics, May 2014; 46: 315 - 327.

Leigh Goedeke et al., A Regulatory Role for MicroRNA 33* in Controlling Lipid Metabolism Gene Expression.Mol. Cell. Biol., Jun 2013; 33: 2339 - 2352.

Park, S. et. al. A ketogenic diet impairs energy and glucose homeostasis by the attenuation of hypothalamic leptin signaling and hepatic insulin signaling in a rat model of non-obese type 2 diabetes. Exp Biol Med, Feb 2011; 236: 194 - 204.

Takimoto & Hamada. Acute exercise increases brain region-specific expression of MCT1, MCT2, MCT4, GLUT1, and COX IV proteins. J Appl Physiol, May 2014; 116: 1238 - 1250.

Wu, S. et. al. Insulin Resistance Secondary to a High-Fat Diet Stimulates Longitudinal Bone Growth and Growth Plate Chondrogenesis in Mice. Endocrinology, Feb 2011; 152: 468 - 475.

Wu. S. et. al. Insulin Resistance Secondary to a High-Fat Diet Stimulates Longitudinal Bone Growth and Growth Plate Chondrogenesis in Mice. Endocrinology, Nov 2010; 10.1210/en.2010-0803.

 

 

艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系,目前已成为众多国际知名品牌的中国总代理或一级代理,主要包括:Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等,可以在第一时间为用户提供最前沿的专业资讯、完备的产品及物流服务。

 

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