worthington 扁桃体组织分离指南

原代细胞分离中,针对扁桃体涉及到扁桃体,扁桃体单核细胞等等的解离通常会使用酶,如何选择合适的酶来完成对应扁桃体的解离,以及原代细胞采集的常规操作, 艾美捷针对扁桃体相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:

worthington 胰腺组织分离指南

 

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

扁桃体
种属 种属详情 细胞名称 酶组合推荐,含推荐浓度 培养基 参考文献
Human 扁桃体 / Tonsillar Collagenase Type 1: 0.2% see reference Muto, M., Manfroi, B., Suzuki, H., Joh, K., Nagai, M., Wakai, S., Righini, C. et al: Toll-Like Receptor 9 Stimulation Induces Aberrant Expression of a Proliferation-Inducing Ligand by Tonsillar Germinal Center B Cells in IgA Nephropathy., J Am Soc Nephrol 28, 1227-1238, 2017
Human 扁桃体 / Tonsillar Collagenase Type 1: 0.1% PBS Schmidt, S., Schenkova, K., Adam, T., Erikson, E., Lehmann-Koch, J., Sertel, S., Verhasselt, B., Fackler, O., Lasitschka, F. and Keppler, O.: SAMHD1's Protein Expression Profile in Humans., J Leukoc Biol 98, 5-14, 2015
Human 扁桃体 / Tonsillar Collagenase Type 4: 0.1% Bambanker Sato, D., Suzuki, Y., Kano, T., Suzuki, H., Matsuoka, J., Yokoi, H., Horikoshi, S., Ikeda, K. and Tomino, Y.: Tonsillar TLR9 Expression and Efficacy of Tonsillectomy with Steroid Pulse Therapy in IgA Nephropathy Patients., Nephrol Dial Transplant 27, 1090-7, 2012
Human, female, 25-45 year 扁桃体单核细胞 / Tonsillar mononuclear cells Collagenase Type 1: 210 u/ml;
Deoxyribonuclease I: 90 u/ml
RPMI Grammer Amrie C, Slota Rebecca, Fischer Randy, Gur Hanan, Girschick Hermann, Yarboro Cheryl, Illei Gabor G, Lipsky PeterE: Abnormal germinal center reactions in systemic lupus erythematosus demonstrated by blockade of CD154-CD40 interactions, J Clin Invest 112, 1506-20, 2003
Human 扁桃体单核细胞 / Tonsillar mononuclear cells Collagenase Type 1: 210 u/ml;
Deoxyribonuclease I: 90 u/ml
RPMI Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE: Expression, regulation, and function of B cell-expressed CD154 in germinal centers, J Immunol 163, 4150-9, 1999
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