worthington 甲状腺/甲状旁腺组织分离指南

原代细胞分离中,针对甲状腺/甲状旁腺涉及到甲状旁腺,甲状腺、甲状腺滤泡、内皮等等的解离通常会使用酶,如何选择合适的酶来完成对应甲状腺/甲状旁腺的解离,以及原代细胞采集的常规操作, 艾美捷针对甲状腺/甲状旁腺相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:

worthington 胰腺组织分离指南

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南


种属 种属详情 细胞名称 酶组合推荐,含推荐浓度 培养基 参考文献
Bovine, adult 甲状旁腺 / Parathyroid Deoxyribonuclease I: 0.005%, HEPES Ham's F10 Nygren, P., Gylfe, E., Larsson, R., Johansson, H., Juhlin, C., Klareskoq, L., Akerstrom, G., and Rastad, J.: Modulation of the Ca2+-Sensing Function of Parathyroid Cells In Vitro and in Hyperparathyroidism, Biochim Biophys Acta 968, 253, 1988
Bovine 甲状旁腺 / Parathyroid glands Deoxyribonuclease I: 0.0075% HEPES buffer Wallace, J., and Scarpa, A.: Regulation of Parathyroid Hormone Secretion in Vitro by Divalent Cations, J Biol Chem 257, 10613, 1982
Bovine 甲状旁腺 / Parathyroid Deoxyribonuclease I: 0.004% Eagle's #2 medium without bicarbonate Brown, E., Hurwitz, S., and Aurbach, G.: Preparation of Viable Isolated Bovine Parathyroid Cells, Endocrinology 99, 1582, 1976
Bovine 甲状腺 / Thyroid Trypsin: 0.004% EBSS Tong, W.: The Isolation and Culture of Thyroid Cells, Meth Enzymol 32, 745, 1974
Chick embryo 甲状腺 / Thyroid Collagenase: 0.25% Tyrode's saline, potassium free Hilfer, S., and Brown, J.: Collagenase. Its Effectiveness as a Dispersing Agent for Embryonic Chick Thyroid and Heart, Exp Cell Res 65, 246, 1971
Chicken, Rhode Island Red, embryo 甲状腺滤泡 / Thyroid follicular Collagenase: 0.2% Tyrode's solution, CMF Spooner, B.: The Expression of Differentiation by Chick Embryo Throid in Cell Culture. I. Functional and Fine Structural Stability in Mass and Clonal Culture, J Cell Physiol 75, 33, 1970
Human 内皮 / Endothelial Collagenase Type 2: 0.1% DMEM Patel, V., Logan, A., Watkinson, J., Uz-Zaman, S., Sheppard, M., Ramsden, J. and Eggo, M.: Isolation and Characterization of Human Thyroid Endothelial Cells., Am J Physiol Endocrinol Metab 284, E168, 2003
Human 甲状腺细胞 / Thyrocytes Collagenase Type 1: 130 u/ml;
Neutral Protease: 0.5 u/ml
HBSS Gianoukakis, A., Cao, H., Jennings, T. and Smith, T.: Prostaglandin Endoperoxide H Synthase Expression in Human Thyroid Epithelial Cells., Am J Physiol Cell Physiol 280, C701, 2001
Human 甲状腺细胞 / Thyrocytes Neutral Protease: 0.5%;

Trypsin: 0.25%;

Collagenase: 0.1%;

EBSS Howie, A., Walker, S., Akesson, B., Arthur, J. and Beckett, G.: Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis., Biochem J 308 ( Pt 3), 713, 1995
Human 甲状腺 / Thyroid Collagenase: 300 u/ml Ham's F-12/MEM Miller, R., Hiraoka, T., Nakamura, N., Tenou, H., Kopecky, K., Jones, M. and Gould, M.: In Vitro Culture of Human Thyroid Cells; Methods and Application to Radiation Biology., J Radiat Res 26, 269, 1985
小鼠 Mouse 甲状腺肿瘤 / Thyroid tumor Collagenase Type 1: 300 u/ml;

Hyaluronidase: 100 u/ml;

Deoxyribonuclease I: 0.01%;

DMEM Kitajima, S., Kohno, S., Kondoh, A., Sasaki, N., Nishimoto, Y., Li, F., Mohammed, M., Muranaka, H., Nagatani, N., Suzuki, M., Kido, Y. and Takahashi, C.: Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts., Stem Cells 33, 1657-69, 2015
小鼠 Mouse, 6 week 甲状腺 / Thyroid Neutral Protease: 0.0012 u/ml;
Collagenase Type 2: 0.25 u/ml
RPMI 1640 Martin, A., Coronel, E., Sano, G., Chen, S., Vassileva, G., Canasto-Chibuque, C., Sedgwick, J., Frenette, P., Lipp, M., Furtado, G. and Lira, S.: A Novel Model for Lymphocytic Infiltration of the Thyroid Gland Generated by Transgenic Expression of the CC Chemokine CCL21., J Immunol 173, 4791, 2004
绵羊 Sheep 甲状腺 / Thyroid Collagenase: 0.2% Puck's Saline F Kerkof, P.: Preparation of Primary Cultures of Ovine Thyroid Gland Cells, J Tiss Cul Meth 7, 23, 1982
Porcine 甲状腺 / Thyroid Trypsin: 0.004% EBSS Tong, W.: The Isolation and Culture of Thyroid Cells, Meth Enzymol 32, 745, 1974
大鼠 Rat, Lewis, male, 4 week 甲状腺 / Thyroid Collagenase Type 2: 0.15%;
Collagenase Type 4: 0.15%
DMEM Arauchi, A., Shimizu, T., Yamato, M., Obara, T. and Okano, T.: Tissue-Engineered Thyroid Cell Sheet Rescued Hypothyroidism in Rat Models After Receiving Total Thyroidectomy Comparing with Nontransplantation Models., Tissue Eng Part A 15, 3943, 2009


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