原代细胞分离中，针对肝脏细胞组织涉及到肝细胞，肝边缘群，肝细胞和血窦内皮，肝脏非实质，各种组织（心脏，肝脏，骨骼，心脏）等等的解离通常会使用酶，如何选择合适的酶来完成对应肝脏细胞组织的解离，以及原代细胞采集的常规操作， 艾美捷针对肝脏细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
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| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 禽类 | Avian, various | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.1% | see reference | Else, P., Brand, M., Turner, N. and Hulbert, A: Respiration Rate of Hepatocytes Varies With Body Mass in Birds, J Exp Biol 207, 2305, 2004 |
| 禽类 | Ducklings, 7 day | 肝细胞 / Hepatocytes | Collagenase: 0.05%； Hyaluronidase: 0.05% |
DMEM/ F12 | Lee J., Culvenor J., Angus P., Smallwood R., Nicoll A., and Locarnini S.: Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells, J Virol 75(16), 7651, 2001 |
| 犬/狗 | Dog, 13 years old (also rat, guinea-pig, rabbit, human) | 肝细胞 / Hepatocytes | Collagenase: 90 u/ml | CF EGTA perfusate | Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981 |
| 犬/狗 | Dog, adult | 肝细胞 / Hepatocytes | Trypsin: 0.1% | HBSS, CMF | Vickrey, H., Ramon, J., and McCann, D.: Continuous Culture of Normal Adult Mammalian Hepatocytes Exhibiting Parenchymal Functions, In Vitro 15, 120, 1979 |
| 鸡 | Chicken, adult | 肝细胞 / Hepatocytes | Collagenase: 0.02% | HEPES, CF | Fraslin, J., Touquette, L., Douaire, M., Menezo, Y., Guillemot, J., and Mallard, J.: Isolation and Long Term Maintenance of Differentiated Adult Chicken Hepatocytes in Primary Culture, In Vitro Cell Dev Biol 28, 615, 1992 |
| 鸡 | Chicken (also rat, SD, 150-250 g) | 肝细胞 / Hepatocytes | Collagenase Type 4: 6000 units； Collagenase Type 3 & 4: |
HEPES | Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars, J Biol Chem 254 (21), 10830, 1979 |
| 鸡 | Chicken, Leghorn, white, 10-15 day | 肝细胞 / Hepatocytes | Deoxyribonuclease I: 0.00125% | PBS | Tarlow, D., Watkins, P., Reed, R., Miller, R., Zwergel, E., and Lane, M.: Lipogenesis and the Synthesis and Secretion of Very Low Density Lipoprotein by Avian Liver Cells in Nonproliferating Monolayer Culture, J Cell Biol 73, 332, 1974 |
| 鸡 | Chick embryos, 5 day | 心，肝 / Heart，Liver | Trypsin: 3.0% | Tyrode's solution, CMF | Steinberg, M.: "ECM": Its Nature, Origin, And Function in Cell Aggregation, Exp Cell Res 30, 257, 1963 |
| 鸡 | Chick embryonic | 各种组织（心脏，肝脏，骨骼，心脏） / Various tissues (heart, liver, skeletal, cardiac) | Trypsin: various grades | CMF Tyrode's solution | Rinaldini, L.: An Improved Method for the Isolation and Quantitative Cultivation of Embryonic Cells, Exp Cell Res 16, 477, 1959 |
| 马 | Equine, 4-13 yr | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.1% | HBSS | Bakala A., Karlik W., and Wiechetek M.: Preparation of Equine Isolated Hepatocytes, Toxicol In Vitro 17(5-6), 615, 2003 |
| 鱼 | Rainbow trout (Oncorhynchus mykiss), male & female, 120-600 g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.045% | Kreb's-Ringer bicarbonate buffer, CF | Blair, J.B., Miller, M.R., Pack, D., Barnes, R., Teh, S.J. and Hinton, D.E.: Isolated Trout Liver Cells:Establishing Short-Term Primary Cultures Exhibiting Cell-to-Cell Interactions, In Vitro Cell Dev Biol 26, 237, 1990 |
| 鱼 | Trout, male, 100 - 200 g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.045% | HBSS with 0.05M HEPES | Lipsky, M., Sheridan, T., Bennett, R., and May, E.: Comparison of Trout Hepatocyte Culture on Different Substrates, In Vitro Cell Dev Biol 22, 360, 1986 |
| 鱼 | Rainbow trout, (Salmo gairdneri), male, 150-200 g | 肝细胞 / Hepatocytes | Collagenase: 100 u/ml | HBSS/CMF | Klaunig, J., Ruch, R., and Goldblah, P.: Trout Hepatocyte Culture: Isolation and Primary Culture, In Vitro Cell Dev Biol 21, 221, 1985 |
| 鱼 | Trout 100g - 5kg | 肝细胞 / Hepatocytes | Hyaluronidase: 0.08% | See reference | Bailey, G., Taylor, M., and Selivonchick, D.: Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner, Carcinogenesis 3, 511, 1982 |
| 鱼 | Trout (Salmo gairdneri), male, 150-200 g；Catfish (Ictalurus punctatus), male, 100 - 150 g | 肝细胞 / Hepatocytes | Collagenase: 100u/ml | HBSS | Klaunig, J.: Establishment of Fish Hepatocyte Cultures For Use in In Vitro Carcinogenicity Studies, Natl Cancer Inst Monogr 65, 163, 1981 |
| 豚鼠 | Guinea-pig, Hartley, male, 250-300 g | 肝细胞 / Hepatocytes | Collagenase Type 2: | Kreb's Ringer bicarbonate buffer | Arinze, I. and Kawai, Y.: Adrenergic Regulation of Glycogenolysis in Isolated Guinea-Pig Hepatocytes: Evidence that B2-Receptors Mediate Catecholamine Stimulation of Glycogenolysis, Arch Biochem Biophys 225, 196, 1983 |
| 豚鼠 | Guinea-pig, young | 肝细胞 / Hepatocytes | Collagenase: 90 u/ml | CF EGTA perfusate | Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.05% | HBSS | Reuschel, E, Jilg, W., Seelbach-Goebel, B. and Demi, L.: Comparative Purification and Characterization of Hepatitis B Virus-like Particles Produced by Recombinant Vaccinia Viruses in Human Hepatoma Cells and Human Primary Hepatocytes, PLoS ONE , , 2019 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.06% | DMEM | Werner, M., Driftmann, S., Kleinehr, K., Kaiser, G., Mathe, Z., Treckmann, J., Paul, A., Skibbe, K., Timm, J., Canbay, A., Gerken, G., Schlaak, J. and Broering, R.: All-In-One: Advanced Preparation of Human Parenchymal and Non-Parenchymal Liver Cells., PLoS ONE 10, e0138655, 2015 |
| 人 | Human | 肝细胞和非实质细胞 / Hepatocytes and non-parenchymal cells | Collagenase Type 1: 0.1% | HBSS | Pfeiffer, E., Kegel, V., Zeilinger, K., Hengstler, J., Nussler, A., Seehofer, D. and Damm, G.: Featured Article: Isolation, Characterization, and Cultivation of Human Hepatocytes and Non-Parenchymal Liver Cells., Exp Biol Med (Maywood) 240, 645-56, 2015 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase: 0.1-0.15% | HEPES | Lee, S., Schelcher, C., Demmel, M., Hauner, M. and Thasler, W.: Isolation of Human Hepatocytes by a Two-Step Collagenase Perfusion Procedure., J Vis Exp , , 2013 |
| 人 | Human, fetal | 肝脏造血 / Liver hematopoietic | Collagenase Type 4: 0.1%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 2 u/ml |
RPMI | Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model., J Vis Exp 70, e4181, 2012 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.05% | Williams E | Torok, E., Lutgehetmann, M., Bierwolf, J., Melbeck, S., Dullmann, J., Nashan, B., Ma, P. and Pollok, J.: Primary Human Hepatocytes on Biodegradable Poly(l-lactic acid) Matrices: A Promising Model for Improving Transplantation Efficiency with Tissue Engineering., Liver Transpl 17, 104, 2011 |
| 人 | Human | 肝干细胞和成肝细胞 / Hepatic stem cells and heptoblasts | Collagenase Type 4: 0.014-0.06% | various | Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance., Methods Cell Biol 86, 137, 2008 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HEPES | Pichard L, Raulet E, Fabre G, Ferrini JB, Ourlin JC, and Maurel P: Human hepatocyte culture, Methods Mol Biol 320, 283, 2006 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase: 0.05% | EBSS | Hughes, R., Mitry, R., Dhawan, A., Lehec, S., Girlanda, R., Rela, M., Heaton, N., and Muiesan, P.: Isolation of Hepatocytes from Livers from Non-Heart-Beating Donors for Cell Transplantation, Liver Transpl 12, 713, 2006 |
| 人 | Human | 肝边缘群 / Hepatic side population | Collagenase: 0.02-0.05% | HBSS | Hussain, S., Strom, S., Kirby, M., Burns, S., Langemeijer, S., Ueda, T., Hsieh, M. and Tisdale, J.: Side Population Cells Derived from Adult Human Liver Generate Hepatocyte-Like Cells In Vitro., Dig Dis Sci 50, 1755, 2005 |
| 人 | Human, adult | 肝细胞 / Hepatocytes | Collagenase: 0.6% | RPMI 1640 | Cho JJ, Joseph B, Sappal BS, Giri RK, Wang R, Ludlow JW, Furth ME, Susick R, and Gupta S.: Analysis of the functional integrity of cryopreserved human liver cells including xenografting in immunodeficient mice to address suitability for clinical applications, Liver Int 24, 361, 2004 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05% | Williams E | Duanmu Z., Locke D., Smigelski J., Wu W., Dahn M., Falany C., Kocarek T., and Runge-Morris M.: Effects of Dexamethasone on Aryl (SULT1A1)- and hydroxysteroid (SULT2A1)-Sulfotransferase Gene Expression in Primary Cultured Human Hepatocytes, Drug Metab Dispos 30(9), 997, 2002 |
| 人 | Human, fetal | 上皮祖细胞 / Epithelial progenitor | Collagenase: 0.03% | DMEM | Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes., J Cell Sci 115, 2679, 2002 |
| 人 | Human (also rat) | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.05% | Leffert's buffer | Dandri, M., Burda, M., Torok, E., Pollok, J., Iwanska, A., Sommer, G., et al.: Repopulation of Mouse Liver with Human Hepatocytes and In Vivo Infection with Hepatitis B Virus, Hepatol 33, 981, 2001 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase: 0.05% | Williams E | Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers, Drug Metab Dispos 28, 1321-6, 2000 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.025% | Williams E | Parzefall, W., Erber, E., Sedivy, R., and Schulte-Hermann, R.: Testing for Induction of DNA Synthesis in Human Hepatocyte Primary Cultures by Rat Liver Tumor Promoters, Cancer Res 51, 1143, 1991 |
| 人 | Human | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HEPES buffer (see reference) | Gomez-Lechon, M., Lopez, P., Donato, T., Montoya, A., Larrauri, A., Gimenez, P., Trullenque, R., Fabra, R., and Castell, J.: Culture of Human Hepatocytes From Small Surgical Liver Biopsies: Biochemical Characterization And Comparison With in vivo, In Vitro Cell Dev Biol 26, 67, 1990 |
| 人 | Human, male, 17-40 yrs. | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HEPES buffer | Begue, J., Baffet, G., Campion, J., and Guillouzo, A.: Differential Response of Primary Cultures of Human and Rat Hepatocytes to Aflatoxin B1-Induced Cytotoxicity and Protection by the Hepatoprotective agent(+)-Cyanidanol-3, Biol Cell 63, 327, 1988 |
| 人 | Human, 4 male kidney donors, age between 17 and 31(also adult rats, male, SD, 180-200 g) | 肝细胞 / Hepatocytes | Collagenase: 0.05% & 0.025% | HEPES (see reference) | LeBot, M., Begue, J., Kernaleguen, D., Robert, J., Ratanasavanh, D., Airiau, J., Riche, C., and Guillouzo, A.: Different Cytotoxicity and Metabolism of Doxorubicin, Daunorubicin, Epirubicin, Esorubicin, and Idarubicin in Cultured Human and Rat Hepatocytes, Biochem Pharmacol 37 (20), 3877, 1988 |
| 人 | Human, 3 human kidney donors, age 20, 23, 25 | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HEPES | Gugen-Guillouzo, C., Campion, J., Brissot, P., Glaise, D., Launois, B., Bourel, M., and Guillouzo, A.: High Yield Preparation of Isolated Human Adult Hepatocytes by Enzymatic Perfusion of the Liver, Cell Biol Int Rep 6 (6), 625, 1982 |
| 人 | Human, 51-75 years old (also rat, guinea-pig, rabbit, dog) | 肝细胞 / Hepatocytes | Collagenase: 90 u/ml | CF EGTA perfusate | Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981 |
| 人 | Human | 肝细胞 / Hepatocytes | Trypsin: 0.1% | HBSS, CMF | Kaighn, M.: Human Liver Cells,Tissue Culture Methods / Applications,(Kruse, P., Patterson, M. eds), , 54, 1973 |
| 猴 | Monkey, Macaca mulatta, 3-5.5 kg | 肝细胞 / Hepatocytes | Collagenase Type 1: 129 u/ml | DMEM/F12 | Weber, A., Groyer-Picard, M.and Dagher, I.: Hepatocyte Transplantation Techniques: Large Animal Models., Methods Mol Biol 481, 83, 2009 |
| 猴 | Monkey (Macaca fascicularis), adult, 5-6 Kg | 肝细胞 / Hepatocytes | Trypsin: 160 u/ml | HEPES buffer | Ulrich, R., Aspar, D., Cramer, C., Kletzien, R., and Ginsberg, L.: Isolation and Culture of Hepatocytes from the Cynogmolgus Monkey (Macaca fascicularis), In Vitro Cell Dev Biol 26, 815, 1990 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05% | DMEM | Cabral, F., Miller, C., Kudrna, K., Hass, B., Daubendiek, J., Kellar, B. and Harris, E.: Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion., J Vis Exp , , 2018 |
| 小鼠 | Mouse, 10-13 week | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.2 u/ml | William's Medium E | Sewing, S., Boess, F., Moisan, A., Bertinetti-Lapatki, C., Minz, T., Hedtjaern, M., Tessier, Y., Schuler, F., Singer, T. and Roth, A.: Establishment of a Predictive In Vitro Assay for Assessment of the Hepatotoxic Potential of Oligonucleotide Drugs., PLoS ONE 11, e0159431, 2016 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase Type 2: 150 u/ml | PBS | Weerasinghe, S., Park, M., Portney, D. and Omary, M.: Mouse Genetic Background Contributes to Hepatocyte Susceptibility to Fas-mediated Apoptosis., Mol Biol Cell 27, 3005-3012, 2016 |
| 小鼠 | Mouse | 肝 / Hepatic | Collagenase Type 4: 0.05% | HBSS | Bigorgne, A., John, B., Ebrahimkhani, M., Shimizu-Albergine, M., Campbell, J. and Crispe, I.: TLR4-Dependent Secretion by Hepatic Stellate Cells of the Neutrophil-Chemoattractant CXCL1 Mediates Liver Response to Gut Microbiota., PLoS ONE 11, e0151063, 2016 |
| 小鼠 | Mouse, 12 week | 肝星状 / Hepatic stellate | Collagenase Type 4: 0.05%； Pronase: 0.04% |
DMEM | Mederacke, I., Dapito, D., Affo, S., Uchinami, H. and Schwabe, R.: High-Yield and High-Purity Isolation of Hepatic Stellate Cells from Normal and Fibrotic Mouse Livers., Nat Protoc 10, 305-15, 2015 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase Type 3: 100 u/ml； Collagenase Type 4: 100 u/ml |
DMEM | Guenthner, C., Luitje, M., Pyle, L., Molyneux, P., Yu, J., Li, A., Leise, T. and Harrington, M.: Circadian Rhythms of Per2::Luc in Individual Primary Mouse Hepatocytes and Cultures., PLoS ONE 9, e87573, 2014 |
| 小鼠 | Mouse | 肝细胞和血窦内皮 / Hepatocytes and sinusoidal endothelial | Collagenase Type 4: 0.05%； Deoxyribonuclease I: 0.003% |
HBSS | Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury., Am J Physiol Gastrointest Liver Physiol 304, G605, 2013 |
| 小鼠 | Mouse | 肝细胞 / hepatocytes | Collagenase Type 4: 100 u/ml | DMEM | Sin, Y., Ballantyne, L., Mukherjee, K., St Amand, T., Kyriakopoulou, L., Schulze, A. and Funk, C.: Inducible Arginase 1 Deficiency in Mice Leads to Hyperargininemia and Altered Amino Acid Metabolism., PLoS ONE 8, e80001, 2013 |
| 小鼠 | Mouse, female, 8-10 week | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.08% | DMEM | Severgnini, M., Sherman, J., Sehgal, A., Jayaprakash, N., Aubin, J., Wang, G., Zhang, L., Peng, C., Yucius, K., Butler, J. and Fitzgerald, K.: A Rapid Two-Step Method for Isolation of Functional Primary Mouse Hepatocytes: Cell Characterization and Asialoglycoprotein Receptor Based Assay Development., Cytotechnology 64, 187-95, 2012 |
| 小鼠 | Mouse | 肝细胞和非实质肝细胞 / Hepatocytes & non-parenchymal liver cells | Collagenase: 0.03-0.05% | DMEM | Brundert, M., Heeren, J., Merkel, M., Carambia, A., Herkel, J., Groitl, P., Dobner, T., Ramakrishnan, R., Moore, K. and Rinninger, F.: Scavenger Receptor CD36 Mediates Uptake of High Density Lipoproteins in Mice and by Cultured Cells., J Lipid Res 52, 745, 2011 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | HIS kit: with modifications | DMEM | Kang, H., Okamoto, K., Kim, Y., Takeda, Y., Bortner, C., Dang, H., Wada, T., Xie, W., Yang, X., Liao, G. and Jetten, A.: Nuclear Orphan Receptor TAK1/TR4-Deficient Mice are Protected Against Obesity-Linked Inflammation, Hepatic Steatosis, and Insulin Resistance., Diabetes 60, 177, 2011 |
| 小鼠 | Mouse | 肝细胞 / Hepatacytes | Collagenase Type 1: 0.05% | Williams E | Holl, D., Kuckenberg, P., Woynecki, T., Egert, A., Becker, A., Huss, S., Stabenow, D., Zimmer, A., Knolle, P., Tolba, R., Fischer, H. and Schorle, H.: Transgenic Overexpression of Tcfap2c/AP-2gamma Results in Liver Failure and Intestinal Dysplasia., PLoS ONE 6, e22034, 2011 |
| 小鼠 | Mouse | CD133+ 干细胞 / CD133+ stem | Collagenase Type 4: 0.05%； Pronase: 0.05%； Deoxyribonuclease I: 0.01% |
DMEM/F12 | Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion., J Vis Exp 56, e3183, 2011 |
| 小鼠 | Mouse, male, 12-14 week | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.03% | Williams E | Chung, S., Timmins, J., Duong, M., Degirolamo, C., Rong, S., Sawyer, J., Singaraja, R., Hayden, M., Maeda, N., Rudel, L., Shelness, G. and Parks, J.: Targeted Deletion of Hepatocyte ABCA1 Leads to VLDL Triglyceride Over-Production and LDL Hypercatabolism, J Biol Chem 285, 12197, 2010 |
| 小鼠 | Mouse | 肝脏非实质 / Liver non-parenchymal | Collagenase Type 3: 100 u/ml | HBSS | Bosschaerts, T., Guilliams, M., Stijlemans, B., Morias, Y., Engel, D., Tacke, F., Herin, M., De Baetselier, P. and Beschin, A.: Tip-DC Development During Parasitic Infection is Regulated by IL-10 and Requires CCL2/CCR2, IFN-gamma and MyD88 Signaling., PLoS Pathog 6, e1001045, 2010 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.075% | HBSS | Shmarakov, I., Fleshman, M., D'Ambrosio, D., Piantedosi, R., Riedl, K., Schwartz, S., Curley, R., von Lintig, J., Rubin, L., Harrison, E. and Blaner, W.: Hepatic Stellate Cells are an Important Cellular Site for β-Carotene Conversion to Retinoid., Arch Biochem Biophys 504, 3-10, 2010 |
| 小鼠 | Mouse, male, adult | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05% | HBSS | Mathijs, K., Kienhuis, A., Brauers, K. J, Jennen, D. , Lahoz, A., Kleinjans, J. and van Delft, J.: Assessing the Metabolic Competence of Sandwich-Cultured Mouse Primary Hepatocytes., Drug Metab Dispos 37, 1305, 2009 |
| 小鼠 | Mouse, male | 肝细胞 / Hepatocytes | Collagenase Type 1: 100 u/ml； Elastase: 0.1 u/ml |
Williams E | Oliva, J., Bardag-Gorce, F., French, B., Li, J., McPhaul, L., Amidi, F., Dedes, J., Habibi, A., Nguyen, S. and French, S.: Fat10 is an Epigenetic Marker for Liver Preneoplasia in a Drug-Primed Mouse Model of Tumorigenesis., Exp Mol Pathol 84, 102, 2008 |
| 小鼠 | Mouse, 7-10 week | 肝内皮 / Liver endothelial | Collagenase: 0.03% | DMEM | Follenzi, A., Benten, D., Novikoff, P., Faulkner, L., Raut, S. and Gupta, S.: Transplanted Endothelial Cells Repopulate the Liver Endothelium and Correct the Phenotype of Hemophilia A Mice., J Clin Invest 118, 935, 2008 |
| 小鼠 | Mouse, 10-12 week | 肝窦内皮细胞 / Liver sinusoidal endothelial cells | Collagenase Type 1: 0.05%； Neutral Protease: 0.025% |
HEPES | Beldi, G., Wu, Y., Sun, X., Imai, M., Enjyoji, K., Csizmadia, E., Candinas, D., Erb, L. and Robson, S.: Regulated Catalysis of Extracellular Nucleotides by Vascular CD39/ENTPD1 is Required for Liver Regeneration., Gastroenterol 135, 1751, 2008 |
| 小鼠 | Mouse | 肝上皮祖细胞 / Liver epithelial progenitor cells | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.05% |
DMEM | Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006 |
| 小鼠 | Mouse | 肝窦内皮 / Liver sinusodial endothelial | Collagenase: 0.03% | DMEM | Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice, Hepatol 42, 140-8, 2005 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.033% | Leffert's buffer | Jiang Guoqiang, Li Zhihua, Liu Franklin, Ellsworth Kenneth, Dallas-Yang Qing, Wu Margaret, Ronan John, Esau Christine, Murphy Cain, Szalkowski Deborah, Bergeron Raynald, Doebber Thomas, Zhang Bei: Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1, J Clin Invest 115, 1030-8, 2005 |
| 小鼠 | Mouse, 8-10 week | 肝源性干细胞 / Liver derived stem cells | Collagenase Type 1: 0.1%； Neutral Protease: 2.4 u/ml |
HBSS | Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity., Blood 106, 1574, 2005 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.053% | DMEM/ F-12 | Sazani P., Gemignani F., Kang S., Maier M., Manoharan M., Persmark M., Bortner D., and Kole R.: Systemically Delivered Antisense Oligomers Up-regulate Gene Expression in Mouse Tissues, Nat Biotechnol 20, 1228, 2002 |
| 小鼠 | Mouse, male, 4-10 week | 肝细胞 / Hepatocytes | Collagenase Type 2: 100 u/ml | HBSS | Lingohr Melissa K, Bull Richard J, Kato-Weinstein Junko, Thrall Brian D: Dichloroacetate stimulates glycogen accumulation in primary hepatocytes through an insulin-independent mechanism, Toxicol Sci 68, 508-15, 2002 |
| 小鼠 | Mouse, male, 8 week | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.04% | Waymouth's medium | Hatano E, Brenner DA: Akt protects mouse hepatocytes from TNF-alpha- and Fas-mediated apoptosis through NK-kappa B activation., Am J Physiol/Gastro 282, G1357, 2002 |
| 小鼠 | Mouse | 肝细胞 / Hepatocytes | Collagenase: 0.05% | Williams E | Donato M, Viitala P, Rodriguez-Antona C, Lindfors A, Castell J, Raunio H, Gomez-Lechon M, Pelkonen O: CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers, Drug Metab Dispos 28, 1321-6, 2000 |
| 小鼠 | Mouse, 7 week | 库普弗细胞/Kupffer / Kupffer cells | Collagenase Type 4: 0.05% | HBSS | Angele MK, Knoferl MW, Schwacha MG, Ayala A, Cioffi WG, Bland KI, and Chaudry IH.: Sex steroids regulate pro- and anti-inflammatory cytokine release by macrophages after trauma-hemorrhage, Am J Physiol 277, C35, 1999 |
| 小鼠 | Mouse | 非实质肝 / Nonparenchymal liver | Collagenase Type 1: 0.05% | Hanks | Ling W, Lougheed M, Suzuki H, Buchan A, Kodama T, Steinbrecher UP: Oxidized or acetylated low density lipoproteins are rapidly cleared by the liver in mice with disruption of the scavenger receptor class A type I/II gene, J Clin Invest 100, 244-52, 1997 |
| 小鼠 | Mouse, C3H, 6-8 weeks | 上皮 / Epithelial | Collagenase: 0.10% | DMEM | Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver, In Vitro 15, 910, 1979 |
| 小鼠 | Mouse, 20-30 g | 实质和非实质细胞 / Parenchymal and non-parenchymal | Hyaluronidase: 0.1% | Hank's w/ Insulin, CMF | Crisp, D., and Pogson, C.: Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver, Biochem J 126, 1009, 1972 |
| 猪 | Porcine, mini-pig, 13 kg | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05%； Neutral Protease: 0.84%； Deoxyribonuclease I: see reference |
Williams E | Meng, F., Chen, Z., Han, M., Hu, X., He, X., Liu, Y., He, W., Huang, W., Guo, H. and Zhou, P.: Porcine Hepatocyte Isolation and Reversible Immortalization Mediated by Retroviral Transfer and Site-Specific Recombination., World J Gastroenterol 16, 1660, 2010 |
| 猪 | Porcine, Yorkshire, male, 21 day | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.07% | Williams E | Terner, M., Gilmore, W.J., Lou, Y. and Squires, E.J.: The Role of CYP2A and CYP2E1 in the Metabolism of 3-Methylindole in Primary Cultured Porcine Hepatocytes., Drug Metab Dispos 34, 848, 2006 |
| 猪 | Porcine, Chinese mini pig, 6-10 kg | 肝细胞 / Hepatocytes | Collagenase Type 4: 125 u/ml | Williams E | Li, J, Li, L., Chao, H., Yang, Q., Liu, X., Sheng, J., Yu, H. and Huang, J: Isolation and Short Term Cultivation of Swine Hepatocytes for Bioartificial Liver Support System., Hepatobiliary Pancreat Dis Int 4, 249, 2005 |
| 猪 | Porcine, one week | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05% | RPMI 1640 | Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich, World J Gastroenterol 10, 699, 2004 |
| 猪 | Porcine, male, 2-3 week | 肝细胞 / Hepatocytes | Collagenase: 0.05% | DMEM | Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures, Am J Physiol Regul Integr Comp Physiol 286, R206-16, 2004 |
| 猪 | Porcine, adult | 肝细胞 / Hepatocytes | Collagenase: 0.05%； Neutral Protease: |
MEM | Zhou X, Liu L, Kano J, Mukaiyama T, and Tokiwa T.: Isolation and Cultivation of Porcine Hepatocytes for Extracorporeal Artificial Liver Support System, Chin Med J 114, 946, 2001 |
| 猪 | Porcine, male, 6-40 kg | 肝细胞 / Hepatocytes | Collagenase: 0.8% | PBS | Gerlach, J., Brombacher, J., Smith, M., Neuhaus, P.: High Yield Hepatocyte Isolation from Pig Livers for Investigation of Hybrid Liver Support Systems: Influence of Collagenase Concentration and Body Weight, J Surg Res 62 (1), 85, 1996 |
| 兔 | Rabbit, New Zealand white | 肝细胞 / Hepatocytes | Collagenase: 90 u/ml | CF EGTA perfusate | Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981 |
| 大鼠 | Rat, SD, 500-700g | 肝细胞和血窦内皮 / Hepatocytes and sinusoidal endothelial | Collagenase Type 4: 0.05%； Deoxyribonuclease I: 0.003% |
HBSS | Perepelyuk, M., Terajima, M., Wang, A., Georges, P., Janmey, P., Yamauchi, M. and Wells, R.: Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury., Am J Physiol Gastrointest Liver Physiol 304, G605, 2013 |
| 大鼠 | Rat, adult | 肝细胞 / Hepatocytes | Collagenase Type 2: 1000 U | William's | Shen, L., Hillebrand, A., Wang, D. and Liu, M.: Isolation and Primary Culture of Rat Hepatic Cells., J Vis Exp 64, e3917, 2012 |
| 大鼠 | Rat, adult | 门静脉成纤维细胞 / Portal fibroblasts | Collagenase Type 2: 0.3% | DMEM/F-12 | Wen, J., Olsen, A., Perepelyuk, M. and Wells, R.: Isolation of Rat Portal Fibroblasts by In Situ Liver Perfusion., J Vis Exp 64, e3669, 2012 |
| 大鼠 | Rat | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.05% | DMEM | Budick-Harmelin, N., Anavi, S., Madar, Z. and Tirosh, O.: Fatty Acids-Stress Attenuates Gluconeogenesis Induction and Glucose Production in Primary Hepatocytes., Lipids Health Dis 11, 66, 2012 |
| 大鼠 | Rat, SD, 240-320g | 肝细胞 / Hepatocytes | HIS kit: per instructions | Waymouth's MB | Pillai, V., and Mehvar, R.: Inhibition of NADPH-Cytochrome P450 Reductase by Tannic Acid in Rat Liver Microsomes and Primary Hepatocytes: Methodological Artifacts and Application to Ischemia-Reperfusion Injury, J Pharm Sci 100, 3495, 2011 |
| 大鼠 | Rat | 肝细胞和 SEC / Hepatocytes and SEC's | Collagenase Type 1: 0.1-0.2% | RPMI | Gopalakrishnan, S. and Harris, E.: In Vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion., J Vis Exp 57, e3138, 2011 |
| 大鼠 | Rat, Wistar, female | 肝星状细胞 / Hepatic stellate cells | Collagenase Type 1: 0.025-0.1%； Pronase: 0.025-0.13% |
DMEM | Zvibel, I., Atias, D., Phillips, A., Halpern, Z. and Oren, R.: Thyroid Hormones Induce Activation of Rat Hepatic Stellate Cells Through Increased Expression of p75 Neurotrophin Receptor and Direct Activation of Rho., Lab Invest 90, 674, 2010 |
| 大鼠 | Rat, SD | 肝星状细胞 / Hepatic stellate cells | Pronase: 0.02%； Collagenase: see reference |
Medium 199 | Handy, J., Saxena, N., Fu, P., Lin, S., Mells, J., Gupta, N. and Anania, F.: Adiponectin Activation of AMPK Disrupts Leptin-Mediated Hepatic Fibrosis via Suppressors of Cytokine Signaling (SOCS-3)., J Cell Biochem 110, 1195, 2010 |
| 大鼠 | Rat, SD, 250-300g | 肝细胞 / Hepatocytes | HIS kit: per instructions | Krebs-Henseleit | Parasrampuria, R. and Mehvar, R.: Dose-Dependent Inhibition of Transporter-Mediated Hepatic Uptake and Biliary Excretion of Methotrexate by Cyclosporine A in an Isolated Perfused Rat Liver Model., J Pharm Sci 99, 5060, 2010 |
| 大鼠 | Rat, SD | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HBSS | Chung, C., Shugrue, C., Nagar, A., Doll, J., Cornwell, M., Gattu, A., Kolodecik, T., Pandol, S. and Gorelick, F.: Ethanol Exposure Depletes Hepatic Pigment Epithelium-Derived Factor, a Novel Lipid Regulator., Gastroenterol 136, 331, 2009 |
| 大鼠 | Rat, Lewis, male | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.1% | DPBS | Bettinger, C., Kulig, K., Vacanti, J., Langer, R. and Borenstein, J.: Nanofabricated Collagen-Inspired Synthetic Elastomers for Primary Rat Hepatocyte Culture., Tissue Eng Part A 15, 1321, 2009 |
| 大鼠 | Rat, 200-300 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.067% | HBSS | Mula, N., Cubero, F., Codesal, J., de Andres, S., Escudero, C., Garcia-Barrutia, S., Millan, I., Arahuetes, R. and Maganto, P.: Survival of Allogeneic Hepatocytes Transplanted into the Thymus., Cells Tissues Organs 188, 270, 2008 |
| 大鼠 | Rat, SD, male, 230-250g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.1% | HEPES | Doleh, L. and Romani, A.: Biphasic Effect of Extra-Reticular Mg2+ on Hepatic G6P Transport and Hydrolysis., Arch Biochem Biophys 467, 283, 2007 |
| 大鼠 | Rat, Lewis, 150-200g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.05% | Williams E | Smith, M. and Mooney, D.: Hypoxia Leads to Necrotic Hepatocyte Death., J Biomed Mater Res A 80, 520, 2007 |
| 大鼠 | Rat, SD, 180-200g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.1% | see reference | Charbonneau, A., Unson, C. and Lavoie, J.: High-Fat Diet-Induced Hepatic Steatosis Reduces Glucagon Receptor Content in Rat Hepatocytes: Potential Interaction with Acute Exercise., J Physiol 579, 255, 2007 |
| 大鼠 | Rat, Wistar, 270-330g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.033% | Williams E | Li, W., Ralphs, K., Slack, J. and Tosh, D.: Keratinocyte Serum-Free Medium Maintains Long-Term Liver Gene Expression and Function in Cultured Rat Hepatocytes by Preventing the Loss of Liver-Enriched Transcription Factors., Int J Biochem Cell Biol 39, 541-54, 2007 |
| 大鼠 | Rat, SD, 500-750 g | 门静脉成纤维细胞 / Portal fibroblasts | Collagenase: 0.03%； Pronase: 0.033%； Hyaluronidase: 0.036% |
DMEM/F-12 | Li, Z., Dranoff, J., Chan, E., Uemura, M., Sevigny, J.and Wells, R.: Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture., Hepatol 46, 1246-56, 2007 |
| 大鼠 | Rat, Fisher, E14 | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.22% | HBSS | Isabel Zvibel, Miri Bronstein, Einav Hubel, Ella Bar-Lev, Zamir Halpern, Ran Oren: Isolation, characterization and culture of Thy1-positive cells from fetal rat livers, World J Gastroenterol 12, 3841-7, 2006 |
| 大鼠 | Rat, Lewis, female, 150-200 g | 肝细胞 / Hepatocytes | Collagenase: see references | DMEM | Sosef MN, Baust JM, Sugimachi K, Fowler A, Tompkins RG, and Toner M.: Cryopreservation of isolated primary rat hepatocytes: enhanced survival and long-term hepatospecific function, Ann Surg 241, 125, 2005 |
| 大鼠 | Rat | 肝窦内皮 / Liver sinusodial endothelial | Collagenase: 0.03% | DMEM | Benten Daniel, Follenzi Antonia, Bhargava Kuldeep K, Kumaran Vinay, Palestro Christopher J, Gupta Sanjeev: Hepatic targeting of transplanted liver sinusoidal endothelial cells in intact mice, Hepatol 42, 140-8, 2005 |
| 大鼠 | Rat, Wistar, 200-300 g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.05% | Williams E | Putz G, Schmider W, Nitschke R, Kurz G, Blum HE: Synthesis of phospholipid-conjugated bile salts and interaction of bile salt-coated liposomes with cultured hepatocytes, J Lipid Res 46, 2325-38, 2005 |
| 大鼠 | Rat, adult | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.1%； Pronase: 1%； Deoxyribonuclease I: 0.007% |
Williams E | Jensen C., Jauho E., Santoni-Rugiu E., Holmskov U., Teisner B., Tygstrup N., and Bisgaard H.: Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1, Am J Pathol 164(4), 1347, 2004 |
| 大鼠 | Rat, SD, male, 200-250 g | 肝细胞 / Hepatocytes | Collagenase: 0.05% | DMEM | Raman Priya, Donkin Shawn S, Spurlock Michael E: Regulation of hepatic glucose metabolism by leptin in pig and rat primary hepatocyte cultures, Am J Physiol Regul Integr Comp Physiol 286, R206-16, 2004 |
| 大鼠 | Rat, Wistar | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05% | RPMI 1640 | Wang Y., Liu H., Guo H., Wen H., and Liu J.: Primary Hepatocyte Culture in Collagen Gel Mixture and Collagen Sandwich, World J Gastroenterol 10, 699, 2004 |
| 大鼠 | Rat, Wistar, 220-270g | 肝细胞 / Hepatocytes | Collagenase Type 1: 200 u/ml | DMEM | Annaert, P., Turncliff, R., Booth, C., Thakker, D. and Brouwer, K.: P-Glycoprotein-Mediated In Vitro Biliary Excretion in Sandwich-Cultured Rat Hepatocytes., Drug Metab Dispos 29, 1277-83, 2001 |
| 大鼠 | Rat, SD, male | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.025% | Williams E | Burczynski, M., McMillian, M., Parker, J., Bryant, S., Leone, A., Grant, E., Thorne, J., Zhong, Z., Zivin, R. and Johnson, M.: Cytochrome P450 Induction in Rat Hepatocytes Assessed by Quantitative Real-Time Reverse-Transcription Polymerase Chain Reaction and the RNA Invasive Cleavage Assay., Drug Metab Dispos 29, 1243, 2001 |
| 大鼠 | Rat, SD, male, 230-250 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.1% | HBSS | Kuddus, R., Nalesnik, M., Subbotin, V., Rao, A., and Gandhi, C.: Enhanced Sythesis and Reduced Metabolism of Endothelin-1 (ET-1) by Hepatocytes - An Important Mechanism of Increased Endogenous Levels of ET-1 in Liver Cirrhosis, J Hepatol 33, 725, 2000 |
| 大鼠 | Rat, male, 3 week | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.02% | Williams E | Low-Baselli A., Hufnagl K., Parzefall W., Schulte-Hermann R., and Grasl-Kraupp B.: Initiated Rat Hepatocytes in Primary Culture: A Novel Tool to Study Alterations in Growth Control During the First Stage of Carcinogenesis, Carcinogenesis 21, 79, 2000 |
| 大鼠 | Rat, SD, male, 450-500 g | 星状 / Stellate | Protease: 0.02% | HBSS | Gabriel, A., Kuddus, R., Rao, A., and Gandhi, C.: Down-Regulation of Endothelin Receptors by Transforming Growth Factor B1 in Hepatic Stellate Cells, J Hepatol 30, 440, 1999 |
| 大鼠 | Rat, Wistar, male | 肝细胞 / Hepatocytes | Collagenase Type 2: 120 u/ml | HBSS | Wolz E, Liechti H, Notter B, Oesterhelt G, Kistler A: Characterization of metabolites of astaxanthin in primary cultures of rat hepatocytes, Drug Metab Dispos 27, 456-62, 1999 |
| 大鼠 | Rat, Lewis, adult | 肝细胞 / Hepatocytes | Collagenase: 60 u/ml | HEPES | Matsuura T, Gad MZ, Harrison EH, and Ross AC: Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocyte and nonparenchymal cell fractions of rat liver, J Nutr 127, 218, 1997 |
| 大鼠 | Rat | 肝细胞 / Hepatocytes | Collagenase: 0.04% - 0.06% | PBS | Alston-Smith, J and Pertoft, H: Isolation of Liver Cells: a System for Obtaining Pure Cells in Monolayer Cultures from a Single Rat Liver,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:14.1, 1995 |
| 大鼠 | Rat | 肝细胞 / Hepatocytes | Collagenase: 0.05% | DMEM | Matsumoto, K and Nakamura, T: Techniques for the Isolation and Cultivation of Hepatocytes using Collagenase,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:16.1, 1995 |
| 大鼠 | Rat, SD, male, 200 g | 肝细胞 / Hepatocytes | Collagenase: 0.05% | Medium 199 | Rana, B., Mischoulon, D., Xie, Y., Bucher, N., and Farmer, S.: Cell-Extracellular Matrix Interactions Can Regulate the Switch between Growth and Differentiation in Rat Hepatocytes: Reciprocal Expression of C/EBPa and Immediate-Early Growth Response Transcription Factors, Mol Cell Biol 14 (9), 5858, 1994 |
| 大鼠 | Rat, Wistar, adult, male, 100-125 g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.05% | Serum-free medium | Liu, J., McKim, J., Liu, Y., and Klaassen, C.: Effects of Butyrate Homologes on Metallothionein Induction in Rat Primary Hepatocyte Cultures, In Vitro Cell Dev Biol 28, 320, 1992 |
| 大鼠 | Rat, SD, male, 250-350 g | 肝细胞 / Hepatocytes | Collagenase Type 4: 200 u/ml | RPMI 1640 | Dixit, V., Arthur, M., and Gitnick, G.: Repeated Transplantation of Microencapsulated Hepatocytes for Sustained Correction of Hyperbilirubinemia in Gunn Rats, Cell Transplant 1, 275, 1992 |
| 大鼠 | Rat, Fisher | 上皮 / Epithelial | Collagenase: 0.05%； Neutral Protease: 0.48%； Hyaluronidase: 0.075% |
Eagles's MEM | Blouin, M., and Marceau, N.: Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells, J Tiss Cul Meth 13, 117, 1991 |
| 大鼠 | Rat, SD, 8 - 12 day | 实质肝细胞 / Parenchymal hepatocytes | Collagenase Type 4: 80 u/ml | HBSS | Davila, J., Reddy, C., Davis, P. and Acosta, D.: Toxicity Assessment of Papaverine Hydrochloride and Papaverine-Derived Metabolites in Primary Cultures of Rat Hepatocytes, In Vitro Cell Dev Biol 26, 515, 1990 |
| 大鼠 | Rat, SD, male, 250-350 g | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.05% | HBSS, CMF | Liu, J., Kershaw, W., and Klaassen, C.: Rat Primary Hepatocyte Cultures are a Good Model for Examining Metallothionein-Induced Tolerance to Cadmium Toxicity, In Vitro Cell Dev Biol 26, 75, 1990 |
| 大鼠 | Rat, Wistar, male, 200 g | 实质细胞 / Parenchymal | Collagenase Type 1: | HEPES, modified | Kindberg, G., Gudmundsen, O., and Berg, T.: The Effect of Vandate on Receptor-mediated Endocytosis of Asialoorosomucoid in Rat Liver Parenchymal Cells, J Biol Chem 265, 8999, 1990 |
| 大鼠 | Rat, SD, male, 175-225 g | 肝细胞 / Hepatocytes | Collagenase: | Kreb's Ringer bicarbonate buffer | Reddy, S., Amick, G., Cooper, R., and Damun, Z.: Insulin Stimulates the Activity of a Protamine in Isolated Rat Hepatocytes, J Biol Chem 265, 7748, 1990 |
| 大鼠 | Rat, SD, neonatal, 8 - 10 days | 肝细胞 / Hepatocytes | Collagenase Type 4: 0.05% | Hanks' BSS, CF | Davila, J., Lenherr, A., and Acosta, D.: Protective Effect of Flavonoids on Drug-Induced Hepatotoxicity In Vitro, Toxicology 57, 267, 1989 |
| 大鼠 | Rat, Fisher, male, 125-250 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.5% | RPMI 1640 | Chan, K., Kost, D., and Michalopoulos, G.: Multiple Sequential Periods of DNA Synthesis and Quiescence in Primary Hepatocyte Cultures Maintained on the DMSO-EGF On/Off Protocol, J Cell Physiol 141, 584, 1989 |
| 大鼠 | Rat, Fischer 344, male, 14 months | 肝细胞 / Hepatocytes | Collagenase: 0.05% | Ringer's biocarbonate buffer | Nagy, I., Ohno-Iwashita, Y., Ohta, M., Nagy, V., Kitani, K., Ando, S., and Imahori, K.: Effect of Perfringolysin O on the Lateral Diffusion Constant of Membrane Proteins of Hepatocytes as Revealed by Fluorescence Recovery After Photobleaching, Biochim Biophys Acta 939, 551, 1988 |
| 大鼠 | Rat, SD, male, 200-300 g | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HEPES | Voss, A. and Sprecher, H.: Metabolism of 6,9,12-Octadecatetraenoic and 6,9,12,15-Octadecatetraenoic Acid, Biochim Biophys Acta 958, 153, 1988 |
| 大鼠 | Rat, Wistar, male,3 month, 200 g | 实质，内皮，Kupffer / Parenchymal，Endothelial，Kupffer | Collagenase Type 1: 0.05% | Krebs Henseleit | Kuiper, J., Zijlstra, F., Kamps, J, and vanBerkel, T.: Identification of Prostaglandin D2 as the Major Eiconsanoid from Liver Endothelial and Kupffer Cells, Biochim Biophys Acta 959, 143, 1988 |
| 大鼠 | Rat, Wistar, male, 200 - 300 g | 实质，Kupffer / Parenchymal，Kupffer | Collagenase Type 2: 0.05% | HBSS with CaCl2 | Cai, H., He, Z., and Ding, Y.: Effects of Monocyte Macrophages Stimulation on Hepatic Lipoprotein Receptors, Biochim Biophys Acta 958, 334, 1988 |
| 大鼠 | Rat, Wistar, female, 17 days | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HBSS | Cotariu, D., Barr-Nea, L., Papo, N., and Zaidman, J.: Induction of gamma-Glutamyl Transferase by Dexamethasone in Cultured Rat Hepatocytes, Enzyme 40, 212, 1988 |
| 大鼠 | Rat, CD strain, albino, male, 140 -180 g | 肝细胞和非实质 / Hepatocytes and Nonparenchymal | Pronase: 0.1% | HEPES buffer with calcium | Braun, L., Mead, J., Panzica, M., Mikumo, R., Bell, G., and Fausto, N.: Transforming Growth Factor B mRNA Increases During Liver Regeneration:A Possible Paracrine Mechanism Of Growth Regulation, Proc Natl Acad Sci U S A 85, 1539, 1988 |
| 大鼠 | Rat, 250-350 g (also guinea-pig & rabbit) | 肝细胞 / Hepatocytes | Collagenase Type 2: 166 u/ml | DMEM | Chang, T., and Chang, C.: Hepatic Uptake of Asialoglycoprotein is Different Among Mammalian Species Due to Different Receptor Distribution, Biochim Biophys Acta 942, 57, 1988 |
| 大鼠 | Rat, SD, male, 180-200 g | 肝细胞 / Hepatocytes | Collagenase: 0.05% | MEM | Gupta, C., Hattori, A., Betschart, J., Virji, M., and Shinozuka, H.: Modulation of Epidermal Growth Factor Receptors in Rat Hepatocytes by Two Liver-Promoting Regimens, a Choline-Deficient and a Phenobarbital Diet, Cancer Res 48, 1162, 1988 |
| 大鼠 | Rat, Fisher 344, 150-200 g | 胆管上皮 / Bile ductular epithelial | Collagenase Type 1: 220 u/ml | L-15 | Mathis, G., Walls, S., and Sirica, A.: Biochemical Characteristics of Hyperplastic Rat Bile Ductular Epithelial Cells Cultured "on Top" and "Inside" Different Extracellular Matrix Substitutes, Cancer Res 48, 6145, 1988 |
| 大鼠 | Rat, Wistar, male, 260-310 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.05% | HEPES | McAbee DD and Weigel PH: ATP-dependent inactivation and reactivation of constitutively recycling galactosyl receptors in isolated rat hepatocytes, Biochemistry 27, 2061, 1988 |
| 大鼠 | Rat, SD, male, 350-400 g | 肝细胞 / Hepatocytes | Collagenase Type 2: | HBSS | Rodriguez de Turco, E., and Spitzer, J.: Kinetics of Diacylglycerol Accumulation in Response to Vasopressin Stimulation in Hepatocytes of Continuously Endotoxaemic Rats, Biochem J 253, 73, 1988 |
| 大鼠 | Rat, SD, male, 350 - 450 g | 脂肪细胞，枯否，正弦内皮细胞 / Lipocytes，Kupffer，Sinusoidal endothelial | Collagenase: 0.015% | DMEM/Ham's F-12 | Friedman, S. and Roll, F.: Isolation and Culture of Hepatic Lipocytes,Kupffer Cells, and Sinusoidal Endothelial Cells by Density Gradient Centrifugation with Stractan, Anal Biochem 161, 207, 1987 |
| 大鼠 | Rat, SD, male | 肝细胞 / Hepatocytes | Collagenase: 100 - 200 µg/g body weight | Eagle's；Eagle's w/HEPES；HBSS | Oka, J. and Weigel, P.: Monensin Inhibits Ligand Dissociation Only Transiently and Partially and distinguishes two galactosyl receptor pathways in isolated rat hepatocytes, J Cell Physiol 133, 243, 1987 |
| 大鼠 | Rat, Fischer, male, 150-250 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 125 - 250 u/ml | MEM (see reference) | Francavilla, A., Ove, P., Polimeno, L., Sciascia, C., Coetzee, M., and Starzi, T.: Epidermal Growth Factor and Proliferation in Rat Hepatocytes in Primary Culture Isolation at Different Times after Partial Hepatectomy, Cancer Res 46, 1318, 1986 |
| 大鼠 | Rat, SD, male, 200-250 g | 肝细胞 / Hepatocytes | Collagenase: 0.05% | HBSS | Kreamer, B., Staecker, J., Sawada, N., Sattler, G., Hsia, M., and Pitot, H.: Use of Low-Speed, Iso-Density Percoll Centrifugation Method to Increase the Viability of Isolated Rat Hepatocyte Preparations, In Vitro Cell Dev Biol 22 (4), 201, 1986 |
| 大鼠 | Rat, SD, female, 100-180 g | 肝细胞 / Hepatocytes | Collagenase: 0.5% | Krebs Ringer bicarbonate buffer | Schwarz, K., Lanier, S., Carter, E., Homcy, C., and Graham, R.: Rapid Reciprocal Changes in Adrenergic Receptors in Intact Isolated Hepatocytes During Primary Cell Culture, Mol Pharmacol 27, 200, 1985 |
| 大鼠 | Rat, Lewis, female, 200-250 g | 肝细胞，Kupffer，内皮细胞 / Hepatocytes, Kupffer, endothelial | Collagenase: 0.05% | Gey's BSS | Holstege A, Leser HG, Pausch J, Gerok W: Uridine catabolism in Kupffer cells, endothelial cells, and hepatocytes, Eur J Biochem 149, 169-73, 1985 |
| 大鼠 | Rat, SD, male, 270 - 320 g | 肝细胞 / Hepatocytes | Collagenase: 0.04% | Bicarbonate buffer with calcium added | Brass, E., Garrity, M., and Robertson, R.: Inhibition of Glucagon-Stimulated Hepatic Glycogenolysis by E-Series Prostaglandins, F.E.B.S. Lett. 169, 293, 1984 |
| 大鼠 | Rat, Wistar, adult, male, 150-200 g | 肝细胞 / Hepatocytes | Trypsin: 0.005% | Williams E | Okumura, T. and Saito, K.: Degradation of Prostaglandin E2 in a Primary Culture of Adult Rat Hepatocytes, J. Biochem. 96, 429, 1984 |
| 大鼠 | Rat, Fischer, Lewis and SD, male 10-18 months | 上皮 / Epithelial | Trypsin: 0.05% | HBSS CMF | Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats, In Vitro 19, 576, 1983 |
| 大鼠 | Rat, Wistar, male, 3 month old | 内皮，枯否，实质 / Endothelial，Kupffer，Parenchymal | Collagenase: 0.05%； Pronase: 0.25% |
HBSS | Nagelkenke, J., Barto, K., and VanBerkel, T.: In vivo and in vitro uptake and degradation of acetylated low density lipoprotein by rat liver endothelial, Kupffer, and parenchymal cells, J Biol Chem 258, 12221, 1983 |
| 大鼠 | Rat, SD, male/female, 250-300 g | 肝细胞 / Hepatocytes | Collagenase: 100 u/ml | Krebs Henseleit bicarbonate buffer | Studer, R. and Borle, A.: Differences between Male and Female Rats in the Regulation of Hepatic Glycogenolysis. The Relative Role of Calcium and cAMP in Phosphorylase Activation by Catecholamines, J Biol Chem 257, 7987, 1982 |
| 大鼠 | Rat, Wistar, 12 day | 上皮 / Epithelial | Trypsin: 0.05% | HBSS, CMF | Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels, Exp Cell Res 131, 363, 1981 |
| 大鼠 | Rat, Zucker, obese | 肝细胞 / Hepatocytes | Collagenase Type 2: 0.30% | Dulbecco-Vogt arginine free Eagle's | Goldstein, A., Palmer, J., and Johnson, P.: Primary Cultures of Fetal Hepatocytes from the Genetically Obese Zucker Rat: Protein Synthesis, In Vitro 17, 651, 1981 |
| 大鼠 | Rat, SD | 肝细胞 / Hepatocytes | Collagenase: 90 u/ml | CF EGTA perfusate | Reese, J. and Byard, J.: Isolation And Culture of Adult Hepatocytes from Liver Biopsies, In Vitro 17, 935, 1981 |
| 大鼠 | Rat, Wistar, female, fetus | 肝细胞 / Hepatocytes | Collagenase: 0.025% | HEPES buffer | Gugen-Guillouzo, C., Tichonicky, L., Szajnert, M., and Kruh, J.: Changes in Some Chromatin and Cytoplastic Enzymes of Perinatal Rat Hepatocytes, In Vitro 16, 1, 1980 |
| 大鼠 | Rat, SD, male, 250-300 g | 实质细胞 / Parenchymal | Collagenase Type 2: 0.05% | Kreb's Henseleit bicarbonate buffer | Yamada, S., Otto, P., Kennedy, D., and Whayne, T.: The Effects of Dexamethasone on Metabolic Activity of Hepatocytes in Primary Monolayer Culture, In Vitro 16, 559, 1980 |
| 大鼠 | Rat, Wistar, male, 180-210 g | 肝细胞 / Hepatocytes | Hyaluronidase: 0.02% | Kreb's buffer | De Gerlache, J., Lans, M., Taper, H., and Roberfroid, M.: Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization, Toxicology 18, 225, 1980 |
| 大鼠 | Rat, SD, 7-10 day | 肝 / Liver | Collagenase: 0.05% | HBSS modified (see reference) | Acosta, D., Anuforo, D., and Smith, R: Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells, J Tiss Cul Meth 6, 35, 1980 |
| 大鼠 | Rat, Wistar, male, 200-250 g | 肝细胞 / Hepatocytes | Hyaluronidase: 460 u/ml | Saline | Poli, G., Gravela, E., Albano, E., and Dianzani, M.: Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion, Exp Mol Pathol 30, 116, 1979 |
| 大鼠 | Rat, SD, female, 100-150 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.065% | DMEM (see reference) | Davis, R., Engelhorn, S., Pangburn, S., Weinstein, D., and Steinberg, D.: Very Low Density Lipoprotein Synthesis and Secretion by Cultured Rat Hepatocytes, J Biol Chem 254 (6), 2010, 1979 |
| 大鼠 | Rat (also chicken) | 肝细胞 / Hepatocytes | Collagenase Type 4: 6000 units； Collagenase Type 3 & 4: |
HEPES | Weigel, P., Schnaar, R., Kuhlenschmidt, M., Schmell, E., Lee, R., Lee, Y., and Roseman, S.: Adhesion of Hepatocytes to Immobilized Sugars, J Biol Chem 254 (21), 10830, 1979 |
| 大鼠 | Rat, SD, male, 200 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 100 u/ml | Buffers 1 & 2 (see reference) | Rubin, K., Kjellen, L., and Oslashbrink, B.: Intercellular Adhesion between Juvenile Liver Cells.. A Method to Measure the Formation of Stable Lateral Contacts Between Cells Attached to a Collagen Gel, Exp Cell Res 109, 413, 1977 |
| 大鼠 | Rat (WAG/RIJ), female, 24, 30, and 37 months | 实质细胞 / Parenchymal | Collagenase Type 1: 0.05% - 0.06% | HEPES buffer | VanBezodijen, C., Grell, T., and Knook, D.: Effect of Age on Protein Synthesis by Isolated Liver Parenchymal Cells, Mech Ageing Dev 6, 293, 1977 |
| 大鼠 | Rat, Fisher, adult, male | 肝细胞 / Hepatocytes | Collagenase Type 1: 100 u/ml | HBSS (see reference) | Williams, G., Bermudez, E., and Scaramuzzino, D.: Rat Hepatocytes Primary Cell Cultures III. Improved Dissociation and Attachment Techniques and the Enchancement of Survival by Culture Medium, In Vitro 13 (12), 809, 1977 |
| 大鼠 | Rat , Wistar, male, 250-300 g | 肝细胞 / Hepatocytes | Collagenase: 0.05% | Hank's solution, CF | Gravela, E., Poli, G., Albano, E., and Dianzani, M.: Studies of Fatty Liver with Isolated Hepatocytes, Exp Mol Pathol 27, 339, 1977 |
| 大鼠 | Rat, Fischer, male, adult, 170-265 g | 肝细胞 / Hepatocytes | Collagenase: 0.05%-0.10% | Williams E | Laishes, B., and Williams, G.: Conditions Affecting Primary Cell Cultures of Functional Adult Rat Hepatocytes. I.. The Effect of Insulin, In Vitro 12, 521, 1976 |
| 大鼠 | Rat, SD, male, 200-250 g | 肝细胞 / Hepatocytes | Collagenase Type 1: 0.05% | MEM | Witters, L., Alberico, L., and Acruch, J.: Insulin Regulation of Glycogen Synthase in the Isolated Rat Hepatocyte, Biochem Biophys Res Commun 69 (4), 997, 1976 |
| 大鼠 | Rat, Fischer, adult, 200-250 g | 上皮 / Epithelial | Hyaluronidase: 0.0075% | KCl-NaCl HEPES Buffer | Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells, Exp Cell Res 89, 139, 1974 |
| 大鼠 | Rat, neonate, 3 day | 肝细胞 / Hepatocytes | Trypsin: 0.25% | PBS, CMF | Bausher, J., and Schaeffer, W.: A Diploid Rat Liver Cell Culture. 1. Characterization and Sensitivity to Aflatoxin B1, In Vitro 9, 286, 1974 |
| 大鼠 | Rat, SD, albino, male | 实质细胞 / Parenchymal | Hyaluronidase: 0.1% | Hank's solution, CMF | Bonney, R., Becker, J., Walker, P., and Potter, V.: Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis, In Vitro 9, 399, 1974 |
| 大鼠 | Rat, embryos, 1-3 days | 肝细胞 / Hepatocytes | Hyaluronidase: 0.10% | HBSS | Gerschenson, L., Berliner, J., and Davidson, M.: The Isolation and Culture of Liver Cells, Vol. 32,, , 733, 1974 |
| 大鼠 | Rat, SD, adult, male, 200-250 g | 实质细胞 / Parenchymal | Hyaluronidase: 0.10% | HBSS, CF | Howard, R., Lee, J., and Pesch, L.: The fine structure, potassium content, and respiratory activity of isolated rat liver parenchymal cells prepared by improved enzymatic techniques, J Cell Biol 57, 642, 1973 |
| 大鼠 | Rat, SD, adult, male, 180-300 g | 实质细胞 / Parenchymal | Collagenase Type 1: 0.05% | Hank's solution, CF | Bissell, D., Hammaker, L., and Meyer, U.: Parenchymal Cells from Adult Rat Liver in Nonproliferating Monolayer Culture. I. Functional Studies, J Cell Biol 59, 722, 1973 |
| 大鼠 | Rat, Wistar, 200-250 g | 肝细胞 / Hepatocytes | Hyaluronidase: 0.10% | HBSS, CF | Berg, T., Boman, D., and Seglen, P.O.: Induction of Tryptophan Oxygenase in Primary Rat Liver Cell Suspensions by Glucocorticoid Hormone, Exp Cell Res 72, 571, 1972 |
| 大鼠 | Rat, SD, female, 130-160 g | 肝细胞 / Hepatocytes | Hyaluronidase: 0.08% | HBSS, CF | Johnson, M., Das, N., Butcher, F., and Fain, J.: The Regulation of Gluconeogenesis in Isolated Rat Liver Cells by Glucagon, Insulin, Dibutyryl Cyclic Adenosine Monophosphate, and Fatty Acids, J Biol Chem 247, 3229, 1972 |
| 大鼠 | Rat, Fisher 344, pregnant (19-21 days gestation), 200-250 g | 肝细胞 / Hepatocytes | Collagenase: 0.3% | Modified Eagle's w/ Serum | Leffert, H., and Paul, D.: Studies on Primary Cultures of Differentiated Fetal Liver Cells, J Cell Biol 52, 559, 1972 |
| 大鼠 | Rat, Wistar, male, 260-310 g | 肝细胞 / Hepatocytes | Collagenase: 0.01 - 0.08% | HEPES | Seglen, P.: Preparation of Rat Liver Cells, Exp Cell Res 74, 450, 1972 |
| 大鼠 | Rat, Fisher, 10 day | 上皮样 / Epithelial-like | Trypsin: 0.25% | PBS | Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver, Exp Cell Res 69, 106, 1971 |
| 大鼠 | Rat, Wistar, male, 6-8 weeks, 80-160 G | 肝细胞 / Hepatocytes | Hyaluronidase: 1.0% | HBSS, CMF | Iype, P.: Cultures from Adult Rat Liver Cells. 1.Establishment of Monolayer Cell-Cultures from Normal Lver, J Cell Physiol 78, 281, 1971 |
| 大鼠 | Rat, 100-200 g | 肝细胞 / Hepatocytes | Hyaluronidase: 0.10% | HBSS, CF | Haung, Y., and Ebner, K.: Induction of Tyrosine Aminotransferase in Isolated Liver Cells, Biochim Biophys Acta 191, 161, 1969 |
| 大鼠 | Rat, SD, adult, 200-300 g | 实质细胞 / Parenchymal | Hyaluronidase: 0.10%； Collagenase Type 1: 0.05% |
HBSS, CF | Berry, M., and Friend, D.: High Yield Preparation of Isolated Rat Liver Parenchymal Cells, J Cell Biol 43, 506, 1969 |
