原代细胞分离中，针对肾脏细胞组织涉及到管段，肾小管、近端小管、肾皮质和肾细胞、肾间充质等等的解离通常会使用酶，如何选择合适的酶来完成对应肾脏细胞组织的解离，以及原代细胞采集的常规操作， 艾美捷针对肾脏细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
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| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 禽类 | Avian, house sparrows | 管段 / Tubule segments | Collagenase Type 2: 0.1% | HBSS | Goldstein D., Reddy V., and Plaga K.: Second Messenger Production in Avian Medullary Nephron Segments in Response to Peptide Hormones, Am J Physiol 276, R847, 1999 |
| 禽类 | Avian, chicken | 肾小管和肾小球 / Tubule and glomeruli | Collagenase Type 1: 0.1% | RPMI 1640 | Reich, C. and Bonar, R.: Separation of Avian Kidney Tubules and Glomeruli for In Vitro Culture, Meth Cell Sci 7, 97, 1982 |
| 牛 | Bovine | 乳头管 / Papillary duct | Hyaluronidase: 0.2% | Keri's buffer；HEPES buffered saline | Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255, F1160, 1988 |
| 犬/狗 | Canine | 肾脂肪来源的细胞 / Renal adipose derived cells | Collagenase Type 1: 0.3% | DMEM | Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011 |
| 犬/狗 | Dog, 1-5 months | 近端小管 / Proximal tubules | Collagenase Type 1: 0.15% | Krebs Ringer bicarbonate buffer | States, B., Reynolds, R., Lee, J., and Segal, S.: Cystine Uptake By Cultured Cells Originating From Dog Proximal Tubule Segments, In Vitro Cell Dev Biol 26, 105, 1990 |
| 犬/狗 | Dog, mongrel, 20 Kg | 近端管状 / Proximal tubular | Deoxyribonuclease I: 0.0125% | (see reference) | Yau, C., Rao, L., and Silverman, M.: Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells, Can J Physiol Pharmacol 63, 417, 1985 |
| 鱼 | Winter flounder (Pseudopleur-onectes americanus) | 肾小管 / Renal tubule | Trypsin: 0.2% | CMF solution | Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport, Am J Physiol 251, 424, 1986 |
| 豚鼠 | Guinea-pig, male, 100 g | 单细胞 / Single cells | Trypsin: 0.25% | CF salt solution | Phillips, H.: Dissociation of Single Cells from Lung or Kidney Tissue with Elastase, In Vitro 8, 101, 1972 |
| 仓鼠 | Hamster | 乳头管 / Papillary duct | Hyaluronidase: 0.2% | Keri's buffer；HEPES buffered saline | Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255, F1160, 1988 |
| 人 | Human | 肾脂肪来源的细胞 / Renal adipose derived cells | Collagenase Type 1: 0.3% | DMEM | Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011 |
| 人 | Human, adult | 肾肿瘤细胞和近端肾小管上皮 / Renal tumor cells and proximal tubular epithelial | Collagenase Type 2: 0.1% | DMEM/F12 | Valente, M., Henrique, R., Costa, V., Jeronimo, C., Carvalho, F., Bastos, M., Guedes de Pinho, P. and Carvalho, M.: A Rapid and Simple Procedure for the Establishment of Human Normal and Cancer Renal Primary Cell Cultures from Surgical Specimens., PLoS ONE 6, e19337, 2011 |
| 人 | Human | 肾细胞 / Renal cells | Collagenase Type 4: 300 u/ml； Neutral Protease: see reference |
HBSS | Presnell, S., Bruce, A., Wallace, S., Choudhury, S., Genheimer, C., Cox, B., Guthrie, K., Werdin, E., Tatsumi-Ficht, P., Ilagan, R., Kelley, R., Rivera, E., Ludlow, J., Wagner, B., Jayo, M. and Bertram, T.: Isolation, Characterization, and Expansion Methods for Defined Primary Renal Cell Populations from Rodent, Canine, and Human Normal and Diseased Kidneys., Tiss Eng 17, 261, 2011 |
| 人 | Human, adult | 肾近曲小管和皮质成纤维细胞 / Renal proximal tubule and cortical fibroblasts | Collagenase Type 2: 383 u/ml | DMEM/F-12 | Johnson, D., Saunders, H., Johnson, F., Huq, S., Field, M., and Pollock, C.: Cyclosporin exerts a direct fibrogenic effect on human tubulointerstitial cells: roles of insulin-like growth factor I, transforming growth factor beta1, and platelet-derived growth factor, J Pharmacol Exp Ther 289, 535-42, 1999 |
| 人 | Human | 肾皮质 / Renal cortex | Trypsin: 0.1% | Tissue Culture Grade Water | McAteer, J, Kempson, S., and Evan, A: Culture of Human Renal Cortex Epithelial Cells, J Tiss Cul Meth 13, 143, 1991 |
| 人 | Human | 乳头管 / Papillary duct | Collagenase: 400 u/ml | Eagle's MEM-HEPES buffer w/L-glutamine | Trifillis, A. and Kahng, M.: Characterization of an In Vitro System of Human Renal Papillary Collecting Duct Cells, In Vitro Cell Dev Biol 26, 441, 1990 |
| 人 | Human | 肾小球系膜 / Mesangial | Trypsin: 0.25% | DMEM/Ham's F-12 | Heieren, M., van der Woude, F., and Balfour Jr., H.: Cytomegalovirus Replicates Efficiently in Human Kidney Mesangial Cells, Proc Natl Acad Sci U S A 85, 1642, 1988 |
| 人 | Human, adult | 管状 / Tubular | Collagenase: 250 u/ml | PBS | Yang, A., Gould-Kostka, J., and Oberley, T.: In Vitro Growth and Differentiation of Human Kidney Tubular Cells on a Basement Membrane Substrate, In Vitro Cell Dev Biol 23 (1), 34, 1987 |
| 人 | Human, adult, 14-66 years | 管状 / Tubular | Collagenase: 100 u/ml | Joklik's MEM | Trifillis, A., Regec, A., and Trump, B.: Isolation, Culture, and Charaterization of Human Renal Tubular Cells, J Urol 133, 324, 1985 |
| 人 | Human | 恶性，间质 / Malignant，Stromal | Papain: 0.009% | Sacks solution | Hemstreet, G., Enoch, P., and Pretlow, T.: Tissue Disaggregation of Human Renal Cell Carcinoma with Further Isopyknic and Isokinetic Gradient Purification, Cancer Res 40, 1043, 1980 |
| 人 | Human, 24 newborn and stillborn | 肾 / Renal | Trypsin: 0.25% | See reference | De Oca, H., and Malinin, T.: Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys, J Clin Microbiol 2, 243, 1975 |
| 猴 | Monkey | 肾 / Kidney | Trypsin: 0.01% - 0.00001% | Eagle's MEM | Melnick, J., and Wallis, C.: Problems Related to the Use of Serum and Trypsin in the Growth of Monkey Kidney Cells, Dev Biol 37, 77, 1976 |
| 小鼠 | Mouse | 巨噬细胞 / Mactrophages | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.01% |
DMEM | Yuan, Y., Li, L., Zhu, L., Liu, F., Tang, X., Liao, G., Liu, J., Cheng, J., Chen, Y. and Lu, Y.: Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy., Stem Cells 38, 639-652, 2020 |
| 小鼠 | Mouse | 肾 / Kidney | Deoxyribonuclease I: 125 u/ml； Protease, Bacillus Licheniformis: 1% |
DPBS | Adam, M., Potter, A. and Potter, S.: Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development., Development 144, 3625-3632, 2017 |
| 小鼠 | Mouse | 集合管上皮 / Collecting duct epithelial | Collagenase Type 2: 0.1% | DMEM | DiRocco, D., Kobayashi, A., Taketo, M., McMahon, A. and Humphreys, B.: Wnt4/β-catenin Signaling in Medullary Kidney Myofibroblasts., J Am Soc Nephrol 24, 1399, 2013 |
| 小鼠 | Mouse | 肾小球 / Glomeruli | Collagenase Type 4: 0.1% | RPMI 1640 | Kabgani, N., Grigoleit, T., Schulte, K., Sechi, A., Sauer-Lehnen, S., Tag, C., Boor, P., Kuppe, C., Warsow, G., Schordan, S., Mostertz, J., Chilukoti, R., Homuth, G., Endlich, N., Tacke, F. and Weiskirchen, R.: Primary Cultures of Glomerular Parietal Epithelial Cells or Podocytes with Proven Origin., PLoS ONE 7, e34907, 2012 |
| 小鼠 | Mouse | 肾小球 / Glomeruli | Collagenase Type 1: 0.1%； Deoxyribonuclease I: 100 u/ml |
DMEM | Sedrakyan, S., Da Sacco, S., Milanesi, A., Shiri, L., Petrosyan, A., Varimezova, R., Warburton, D., Lemley, K., De Filippo, R. and Perin, L.: Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis., J Am Soc Nephrol 23, 661-73, 2012 |
| 小鼠 | Mouse, 10-14 week | 近端小管 / Proximal tubule | Collagenase Type 1: 0.1% | See reference | Park, J., Pasupulati, R., Feldkamp, T., Roeser, N. and Weinberg, J.: Cyclophilin D and the Mitochondrial Permeability Transition in Kidney Proximal Tubules after Hypoxic and Ischemic Injury., Am J Physiol/Renal 301, F134, 2011 |
| 小鼠 | Mouse, embryonic | 肾 / Kidney | Collagenase Type 1: 0.25%； Pancreatin: 1.0%； Deoxyribonuclease I: 1 u/ml |
HBSS | Brown, A., Blank, U., Adams, D., Karolak, M., Fetting, J., Hill, B. and Oxburgh, L.: Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney., J Vis Exp 50, e2555, 2011 |
| 小鼠 | Mouse, adult | 肾球来源 / Kidney sphere-derived | Collagenase Type 4: 0.1% | Iscove's Modified Dulbecco's | Huang, Y., Johnston, P., Zhang, B., Zakari, A., Chowdhry, T., Smith, R., Marban, E., Rabb, H. and Womer, K.: Kidney-Derived Stromal Cells Modulate Dendritic and T Cell Responses., J Am Soc Nephrol 20, 831-41, 2009 |
| 小鼠 | Mouse, male, 8-16 week | 肾小管上皮 / Renal tubular epithelial | Collagenase: 200 u/ml； Soybean Trypsin Inhibitor: see reference |
HBSS | Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress., Oxid Med Cell Longev 1, 33, 2008 |
| 小鼠 | Mouse | 近端小管 / Proximal tubule | Collagenase Type 2: 0.013% | see reference | Wright, J., Morales, M., Sousa-Menzes, J., Ornellas, D., Sipes, J., Cui, Y., Cui, I., Hulamm, P., Cebotaru, V., Cebotaru, L., Guggino, W. and Guggino, S.: Transcriptional Adaptation to Clcn5 Knockout in Proximal Tubules of Mouse Kidney., Physiol Genomics 33, 341, 2008 |
| 小鼠 | Mouse, 4 week | 内皮肾 / Endothelial kidney | Collagenase Type 1: 0.1% | DMEM | Kondo, S., Scheef, E., Sheibani, N. and Sorenson, C.: PECAM-1 Isoform-Specific Regulation of Kidney Endothelial Cell Migration and Capillary Morphogenesis., Am J Physiol Cell Physiol 292, C2070, 2007 |
| 小鼠 | Mouse, adult | 皮质，近端小管 / Cortex, proximal tubule | Collagenase: 0.15% | DMEM | Syal Ashu, Schiavi Susan, Chakravarty Sumana, Dwarakanath Vangipuram, Quigley Raymond, Baum Michel: Fibroblast growth factor-23 increases mouse PGE2 production in vivo and in vitro, Am J Physiol/Renal 290, F450-5, 2006 |
| 小鼠 | Mouse | 肾间充质 / Kidney mesenchymal | Collagenase: 250 u/ml； Neutral Protease: 33.3 u/ml |
DMEM/F12 | Plotkin, M. and Goligorsky, M.: Mesenchymal Cells from Adult Kidney Support Angiogenesis and Differentiate into Multiple Interstitial Cell Types including Erythropoietin-Producing Fibroblasts., Am J Physiol Renal Physiol 291, F902-12, 2006 |
| 小鼠 | Mouse, 10-12 week | 肾 / Kidney | Collagenase Type 4: 0.075% | DMEM | Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney., J Am Soc Nephrol 17, 3300, 2006 |
| 小鼠 | Mouse, male, 12-16 week | 肾近端小管细胞 / Renal proximal tubule cells | Collagenase Type 2: 0.1%； Soybean Trypsin Inhibitor: 0.25% |
DMEM/F-12 | Cunningham, R., Xiaofei, E., Steplock, D., Shenolikar, S. and Weinman, E.: Defective PTH Regulation of Sodium-Dependent Phosphate Transport in NHERF-1-/- Renal Proximal Tubule Cells and Wild-Type Cells Adapted to Low-Phosphate Media., Am J Physiol Renal Physiol 289, F933, 2005 |
| 小鼠 | Mouse, male, 2 month | 皮质集合管 / Cortical collecting duct | Collagenase: 54-178 u/ml； Protease: see reference |
MEM | Sindic, A., Velic, A., Basoglu, C., Hirsch, J., Edemir, B., Kuhn, M. and Schlatter, E.: Uroguanylin and Guanylin Regulate Transport of Mouse Cortical Collecting Duct Independent of Guanylate Cyclase C., Kidney Int 68, 1008, 2005 |
| 小鼠 | Mouse, 6 week | 肾小球系膜细胞 / Glomerular mesangial cells | Collagenase Type 4: 0.1% | RPMI 1640 | Radeke HH, Janssen-Graalfs I, Sowa EN, Chouchakova N, Skokowa J, Loscher F, Schmidt RE, Heeringa P, Gessner JE.: Opposite regulation of type II and III receptors for immunoglobulin G in mouse glomerular mesangial cells and in the induction of anti-glomerular basement membrane (GBM) nephritis, J Biol Chem 277(30), 27535, 2002 |
| 小鼠 | Mouse, 3-7 month | 成纤维细胞，系膜，平滑肌 / Fibroblasts, mesangial, smooth muscle | Trypsin: 0.25%； Collagenase: see reference； Soybean Trypsin Inhibitor: 0.05% |
DMEM | Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH: Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation, Mol Biol Cell 10, 1569-79, 1999 |
| 小鼠 | Mouse | 上皮 / Epithelial | Collagenase Type 4: 1% | DMEM /F-12 | Taub, M: Renal Tubule Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 2,Doyle, A., Griffiths, J., and Newell, D., John Wiley & Sons, Ltd., 14B:6.1, 1995 |
| 小鼠 | Mouse, naive SJL (H-2S) | 近端肾小管上皮 / Proximal tubular epithelial | Deoxyribonuclease I: 15 �g/ml | RPMI 1640 | Haverty, T., Kelly, C., Hines, W., Amenta, P., Watanabe, M., Harper, R., Kefalides, N., and Neilson, E.: Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis, J Cell Biol 107, 1359, 1988 |
| 猪 | Porcine, hybrid | 肾 / Kidney | Collagenase Type 1: 0.1% | HBSS | Heussner, A. and Dietrich, D.: Primary Porcine Proximal Tubular Cells as an Alternative to Human Primary Renal Cells in Vitro: an Initial Characterization., BMC Cell Biol 14, 55, 2013 |
| 兔 | Rabbit | 肾近端管状 / Renal proximal tubular | Collagenase Type 1: 60 u/ml | DMEM/F12 | Nowak, G. and Bakajsova, D.: Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes., J Vis Exp 71, e4301, 2013 |
| 兔 | Rabbit, New Zealand | 近端小管细胞 / Proximal tubule cells | Collagenase Type 4: 0.005%； Soybean Trypsin Inhibitor: 0.00025% |
DMEM/F-12 | Taub, M.: Primary Kidney Proximal Tubule Cells., Methods Mol Biol 290, 231, 2005 |
| 兔 | Rabbit, New Zealand White, female | 肾单位段 / Nephron segments | Collagenase Type 2: 0.05%； Deoxyribonuclease I: 50 u/ml |
Eagle's MEM | Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am J Physiol 273, F650, 1997 |
| 兔 | Rabbit, New Zealand white, male, 1.5 - 2.0 Kg | 导管 / Duct | Soybean Trypsin Inhibitor: 0.025% | Hank's Solution with calcium and HEPES | Naray-Fejes-Toth, A., and Fejes-Toth, G.: Immunoelection and Culture of Cortical Collecting Duct Cells, J Tiss Cul Meth 13, 179, 1991 |
| 兔 | Rabbit, New Zealand white, female, 2-3 Kg | 肾近端小管 / Renal proximal tubules | Deoxyribonuclease I: 70 u/ml | Modified DME-F12 | Rodeheaver, D., Aleo, M., and Schnellmann, R.: Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation, In Vitro Cell Dev Biol 26, 898, 1990 |
| 兔 | Rabbit | 乳头管 / Papillary duct | Hyaluronidase: 0.2% | Keri's buffer | Husted, R., Hayashi, M., and Stokes, J.: Characteristics of Papillary Collecting Duct Cells in Primary Culture, Am J Physiol 255, F1160, 1988 |
| 兔 | Rabbit, New Zealand, white, 2-3 kg | MTALH细胞，RCCT细胞 / MTALH cells，RCCT cells | Collagenase: 0.1% | DMEM | Allen, M., Nakao, A., Sonnenburg, W., Burnatowska-Hledin, M., Speilman, W., and Smith, W.: Immunodissection of Cortical and Medullary Thick Ascending Limb Cells From Rabbit Kidney, Am J Physiol 255, F704, 1988 |
| 兔 | Rabbit, New Zealand, white | 集合管 / Collecting tubule | Trypsin: 0.05% | Kreb's Ringer buffer | Grenier, F., and Smith, W.: Formation of 6-keto-PGF-1aby Collecting Tubule Cells Isolated from Rabbit Renal Papillae, Prostaglandins 16, 759, 1978 |
| 兔 | Rabbit, New Zealand, white, female, 2.5 Kg | 乳头集合管 / Papillary collecting duct | Collagenase: 0.2% | (see reference) | Dworzack, D., and Grantham, J.: Preparation of Renal Papillary Collecting Duct Cells for Study, Nat Neurosci 8, 191, 1975 |
| 大鼠 | Rat, SD, fetal | 胎肾 / Fetal kidney | Collagenase Type 4: 0.1% | MEM | Gupta, A., Jadhav, S., Tripathy, N. and Nityanand, S.: Fetal Kidney Cells Can Ameliorate Ischemic Acute Renal Failure in Rats through Their Anti-Inflammatory, Anti-Apoptotic and Anti-Oxidative Effects., PLoS ONE 10, e0131057, 2015 |
| 大鼠 | Rat, Lewis, male | 肾脂肪来源的细胞 / Renal adipose derived cells | Collagenase Type 1: 0.3% | DMEM | Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011 |
| 大鼠 | Rat | 近端小管 / Proximal tubule | Collagenase Type 2: 0.1% | see reference | Panico, C., Luo, Z., Damiano, S., Artigiano, F., Gill, P. and Welch, W.: Renal Proximal Tubular Reabsorption is Reduced in Adult Spontaneously Hypertensive Rats: Roles of Superoxide and Na+/H+ Exchanger 3., Hypertension 54, 1291, 2009 |
| 大鼠 | Rat, SD, male, 200-250g | 髓质粗升支 / Medullary thick ascending limb | Collagenase: 0.1% | HEPES-saline | Silva, G., Beierwaltes, W., and Garvin, J.: Extracellular ATP stimulates NO production in rat thick ascending limb, Hypertension 47, 563-7, 2006 |
| 大鼠 | Rat, Wistar, 300g | 肾近端小管 / Renal proximal tubules | Collagenase Type 2: 0.2% | DMEM/F12 | Deng Aihua, Miracle Cynthia M, Lortie Mark, Satriano Joseph, Gabbai Francis B, Munger Karen A, Thomson Scott C, Blantz Roland C: Kidney oxygen consumption, carbonic anhydrase, and proton secretion, Am J Physiol/Renal 290, F1009-15, 2006 |
| 大鼠 | Rat, Wistar, male, 300-350 g | 肾近端小管 / Renal proximal tubules | Collagenase Type 2: 0.2% | DMEM/F-12 | Deng, A., Miracle, C., Suarez, J., Lortie, M., Satriano, J., Thomson, S., Munger, K. and Blantz, R.: Oxygen Consumption in the Kidney: Effects of Nitric Oxide Synthase Isoforms and Angiotensin II., Kidney Int 68, 723, 2005 |
| 大鼠 | Rat | 皮质小管细胞 / Cortical tubule cells | Collagenase: 0.6% | DMEM | Arystarkhova, E., Wetzel R., and Sweadner K.: Distribution and Oligomeric Association of Splice Forms of Na(+)-K(+)-ATPase Regulatory Gamma-subunit in Rat Kidney, Am J Physiol/Renal 282(3), F393, 2002 |
| 大鼠 | Rat, SD, male 275-300 g | 肾小球系膜细胞 / Glomerular mesangial cells | Collagenase Type 1: 250 u/ml | DMEM | Amiri Farhad, Shaw Sean, Wang Xiaodan, Tang Jie, Waller Jennifer L, Eaton Douglas C, Marrero Mario B: Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose, Kidney Int Suppl 61, 1605-16, 2002 |
| 大鼠 | Rat, SD, 1 week | 皮质收集管 / Cortical collection duct | Collagenase Type 2: 0.15% | DMEM/Ham's F12 | Valencia L., Bidet M., Martial S., Sanchez E., Melendez E., Tauc M., Poujeol C., Martin D., Namorado M., Reyes J., and Poujeol P.: Nifedipine-activated Ca(2+) Permeability in Newborn Rat Cortical Collecting Duct Cells in Primary Culture, Am J Physiol Cell Physiol 280(5), C1193, 2001 |
| 大鼠 | Rat, SD, 250-300 g | 微血管 / Microvessels | Collagenase Type 2: 0.1% | PSS | Li, N., Teggatz, E., Li, P., Allaire, R., and Zou, A.: Formation and Actions of Cyclic ADP-Ribose in Renal Microvessels, Microvasc Res 60, 149, 2000 |
| 大鼠 | Rat, SD, 250-300 g | 肾 / Renal | Collagenase Type 2: 0.2% | see reference | Mattson D., and Wu F.: Nitric Oxide Synthase Activity and Isoforms in Rat Renal Vasculature, Hypertension 35, 337, 2000 |
| 大鼠 | Rat, SD, male, 5-6 week | 肾小管 / Renal tubules | Collagenase: 0.1% | see reference | Miyata, N., Park, F., Li, X., and Cowley, A.: Distribution of Angiotensin AT1 and AT2 Receptor Subtypes in the Rat Kidney, Am J Physiol 277, F437, 1999 |
| 大鼠 | Rat, SD, 225-250 g | 肾小球系膜细胞 / Glomerular mesangial cells | Collagenase Type 1: 250 u/ml | DMEM | Amiri F, Garcia R: Regulation of angiotensin II receptors and PKC isoforms by glucose in rat mesangial cells, Am J Physiol 276, F691-9, 1999 |
| 大鼠 | Rat, SD, male | 近端小管悬液 / Proximal tubule suspensions | Collagenase Type 4: 0.1%； Pronase E: 2 u/ml |
HEPES buffer | Eitle E, Hiranyachattada S, Wang H, Harris PJ: Inhibition of proximal tubular fluid absorption by nitric oxide and atrial natriuretic peptide in rat kidney, Am J Physiol 274, C1075-80, 1998 |
| 大鼠 | Rat, SD, male | 肾单位段 / Nephron segments | Collagenase Type 2: 0.05%； Deoxyribonuclease I: 50 u/ml |
Eagle's MEM | Schafer, J., Watkins, M., Li, L., Herter, P., Haxelmans, S., and Schlatter, E.: A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments, Am J Physiol 273, F650, 1997 |
| 大鼠 | Rat, 12-weeks-old | 肾 / Renal | Collagenase: 0.1% | DMEM | Ishikawa, S., Kusaka, I., Higashiyama, M., Nagasaka, S., Saito, T., Honda, K., and Saito, T.: Cellular Signaling and Proliferative Action of AVP in Mesangium of SHR: Effect of Low Density Lipoprotein, Nat Neurosci 50, 1506, 1996 |
| 大鼠 | Rat, male, 150-200 g | 上皮，近端小管 / Epithelial，Proximal tubule | Protease: 0.1% | HBSS/CMF | Elliget, K., and Trump, B.: Primary Cultures of Normal Rat Kidney Proximal Tubule Epithelial Cells for Studies of Renal Cell Injury, In Vitro Cell Dev Biol 27, 739, 1991 |
| 大鼠 | Rat, adult | 内髓集合管 / Inner medullary collecting duct | Collagenase Type 2: 0.1% | PBS | Brion, L., Schwartz, J., Lachman, H., Zavilowitz, B., and Schwartz, G.: Development of H+ Secretion by Cultured Renal Inner Medullary Collecting Duct Cells, Am J Physiol 257, F486, 1989 |
| 大鼠 | Rat, Wistar, female, 100-150 g | 肾小球系膜 / Glomerular mesangial | Collagenase: 0.025% | RPMI 1640 | Wang, J., Kester, M., and Dunn, M.: The Effects of Endotoxin on Platelet-Activating Factor Synthesis in Cultured Rat Glomerular Mesangila Cells, Biochim Biophys Acta 969, 217, 1988 |
| 大鼠 | Rat, Wistar, male, 150 - 200 g | 肾脏靶点 / Renal target | Collagenase: 1.0% (also 0.7%) | Eagle's MEM | Barlet-Bas, C., Khadouri, C., Marsey, S., and Doucet, A.: Sodium-Independent In Vitro Induction of Na+,K+-ATPase by Aldosterone in Renal Target Cells: Permissive Effect of Triiodothyronine, Proc Natl Acad Sci U S A 85, 1707, 1988 |
| 大鼠 | Rat, Wistar, male, adult | 近端小管 / Proximal tubules | Collagenase: 0.2% | Krebs-Henseleit buffer | Gesek, F., Wolff, D., and Strandhov, J.: Improved Separation Method for Rat Proximal and Distal Renal Tubules, Am J Physiol 253, F358, 1987 |
| 大鼠 | Rat,Wistar, 300-400 gm | 近端小管 / Proximal tubules | Collagenase: 0.15 % | Krebs Henseleit solution | Vinay, P., Gougoux, A., and Lemieux, G.: Isolation of a Pure Suspension of Rat Proximal Tubules, Am J Physiol 241, F403, 1981 |
| 大鼠 | Rat | 成纤维细胞，肾脏 / Fibroblasts，Kidney | Trypsin: up to 0.25% | Dulbecco-Vogt MEM | Wallach, D., Anderson, W., and Pastan, I.: Activation of Adenylate Cyclase in Cultured Fibroblasts by Trypsin, J Biol Chem 253, 24, 1978 |
