总抗氧化能力（TAC）检测，Cell Biolabs试剂盒助力科研！

活性氧(Reactiveoxygenspecies,ROS)主要包括羟基自由基、超氧自由基和过氧化氢。在细胞或组织的正常生理代谢过程中会产生活性氧，同时一些环境因子例如紫外照射、吸烟、环境污染等也可以诱导活性氧的产生。活性氧在体内的失衡，将会导致细胞脂、蛋白和DNA的氧化损伤，继而导致各种肿瘤、风湿性关节炎、糖尿病、肝损伤、以及中枢神经系统疾病等。

当然，机体中也存在许多种抗氧化的物质，包括抗氧化大分子、抗氧化小分子和酶等，能够清除体内产生的各种活性氧，以阻止活性氧造成的氧化损伤。一个体系内的各种抗氧化大分子、抗氧化小分子和酶的总的水平即体现了该体系内的总抗氧化能力。因此测定样本中的总抗氧化能力（TAC）具有非常重要的生物学意义。

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Fig. 试剂盒原理图

* 本产品仅适用于科研用途.

FAQ常见问题：

1. 总抗氧化能力（TAC）检测试剂盒，有物种种属限制吗？

我们的总抗氧化能力（TAC）检测试剂盒，适用于任何生物蛋白质样品。因为试剂盒原理并不依赖于可能因物种而异的蛋白质结构。

2. 是否可以检测冻存过的样本？

我们的总抗氧化能力（TAC）检测试剂盒，通常适用于在 -80ºC 下保存两个月以内样品，但我们建议尽可能测试新鲜样品以获得最佳结果。

3. 在制备食品样品时，1:2, w/v 是什么意思？

食品样品应准备为 1 g 样品：2 mL 去离子水。

4. 柠檬酸盐血浆样品可以用于该测定吗？

柠檬酸盐血浆样品与总抗氧化能力测定兼容。 但应避免使用EDTA，因为这会干扰检测。

5. 该试剂盒可以测量细胞培养上清液的总抗氧化能力吗？

不幸的是，我们的总抗氧化能力测定与细胞培养上清液不兼容。该测定基于铜的还原，介质中存在的铁会干扰该反应。

结果展示：

试剂盒标准曲线

近期发表文章：

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