经过CiteAb权威验证,开曼生物SOD检测试剂盒,值得信赖!

 

相信各位科研er对于CiteAb并不陌生,CiteAb是全球著名的抗体,蛋白,试剂盒搜索引擎,所有产品检索结果全部按照文献引用数量进行排名,产品供货商无法用付费来提升排名,确保产品排名完全公开透明。

 

近期,CiteAb发布了关于2020年全球引用文献最多的100款试剂盒的调查报告,定睛一看发现我们代理的这款超氧化物歧化酶(SOD)检测试剂盒赫然在列,心想,这么好的试剂盒不让大家知道真是太可惜啦,今天就给大家介绍下SOD以及这款2021年引文数量全球第一的SOD检测试剂盒吧!

 

(报告详情:https://blog.citeab.com/which-were-the-top-100-kits-and-assays-of-2020/)

SOD检测试剂盒

(这款报告是基于全球市场超过700000种试剂盒和超过150000次引用进行整理的。)

 

超氧化物歧化酶(SOD):

超氧化物歧化酶又称超氧歧化酶。是一类能催化超氧阴离子自由基(O2-)歧化为H2O2和O2的酶。此酶分布极为广泛,迄今已从细菌、真菌、藻类、植物、原生动物、昆虫、鱼类和哺乳动物等各种生物体内分离到。

 

超氧歧化酶十分稳定,牛红细胞SOD在75℃下加热数分钟仍很少失活,对酸碱也较稳定,可在pH 5.3~10.5范围内反应。

 

SOD的分类:

超氧歧化酶是一类金属酶,可分为三种类型:

  • 第一种含铜和锌,称含铜、锌超氧歧化酶,呈蓝绿色,主要存在于真核细胞的细胞质中;
  • 第二种含锰,称含锰超氧歧化酶,呈紫色,主要存在于原核细胞及真核细胞的基质(如线粒体等)中;
  • 第三种含铁,称含铁超氧歧化酶,呈黄褐色,主要存在于原核细胞及少数植物细胞中。

 

SOD能够催化超氧阴离子自由基歧化生成氧和过氧化氢,在机体氧化与抗氧化平衡中起到至关重要的作用,与很多疾病的发生、发展密不可分,因此SOD成为了许多生理过程的重要标志物,检测准确检测样本中SOD的量显得尤为重要了。

 

今天给大家带来的这款艾美捷科技代理的美国开曼生物公司生产的超氧化物歧化酶(SOD)检测试剂盒(706002),是经过CiteAb权威验证的,被众多研究人员信赖并选择的一款SOD检测试剂盒(2020年引文数量全球第一

SOD检测试剂盒

超氧化物歧化酶(SOD)检测试剂盒检测原理

超氧化物歧化酶(SOD)检测试剂盒检测
这款SOD检测试剂盒使用四氮唑盐检测黄嘌呤氧化酶和次黄嘌呤产生的超氧化物自由基

 

优势特点:

样本类型:血清,血浆,红和细胞裂解液,组织匀浆,细胞裂解液,线粒体

精确度:组内CV:3.2%,组间CV:3.7%

检测范围:0.005-0.05 units/ml(一个unit定义:50%超氧自由基歧化所需的酶量。)

检测SOD的类型:可检测所有三种类型SOD(Cu/Zn、Mn和FeSOD)

 

测试过的干扰物质:

类型 试剂 是否会干扰检测
缓冲液 Tris No
硼酸酯 Yes
Hepes No
磷酸盐 No
洗涤剂 SDS(≤0.1%)) Yes
Triton X-100 (≤0.1%) No
聚山梨醇酯 20(≤0.1%) Yes
CHAPS (0.1%) Yes
蛋白酶/抑制剂/螯合剂 Antipain (0.1 mg/ml) Yes
PMSF (1 mM) Yes
Leupeptin (1 mg/ml) Yes
胰蛋白酶 (0.1 mg/ml) Yes
凝乳抑素 (0.1 mg/ml) Yes
EGTA (≤1 mM) No
EDTA (≤1 mM) No
DMSO 乙醇(10ul) No
甲醇(10ul) Yes
DMSO(10ul) Yes
其他 Glutathione (≤1.5 mM) No
Glycerol (≤1%) No
BSA (≤1%) No
Dithiothreitol (3 mM) Yes

 

产品名称 规格 货号
中文名称:超氧岐化酶分析试剂盒-
英文名称:Superoxide Dismutase Assay Kit
480 Assays 706002-480
中文名称:超氧岐化酶分析试剂盒-96次分析
英文名称:Superoxide Dismutase Assay Kit
96 Assays 706002-96

 

部分引用文献赏析:

  1. Ouyang, B., Li, Z., Ji, X., et al. The protective role of lutein on isoproterenol-induced cardiac failure rat model through improving cardiac morphology, antioxidant status via positively regulating Nrf2/HO-1 signalling pathway. Pharm. Biol. 57(1), 529-535 (2019).
  2. Wiecek, M., Szymura, J., Maciejczyk, M., et al. Anaerobic Exercise-Induced Activation of Antioxidant Enzymes in the Blood of Women and Men. Front. Physiol. 9, 1006 (2018).
  3. Chang, C.-W., Chen, C.-Y., Yen, C.-C., et al. Repressed exercise-induced hepcidin levels after danggui buxue tang supplementation in male recreational Nutrients 10(9), E1318 (2018).
  4. Wen, J.J., and Garg, N.J. Manganese superoxide dismutase deficiency exacerbates the mitochondrial ROS production and oxidative damage in Chagas disease. PLoS Negl. Trop. Dis. 12(7), e0006687 (2018).
  5. Lee, H.Y., Kim, S.W., Lee, G.H., et al. Curcumin and Curcuma longa L. extract ameliorate lipid accumulation through the regulation of the endoplasmic reticulum redox and ER stress. Sci. Rep. 7(1), 6513 (2017).
  6. Wang, M.-Y., Srinivasan, M., Dasari, D., et al. Antioxidant activity of Yichun Blue Honeysuckle (YBHS) berry counteracts CCl4-Induced toxicity in liver injury model of Antioxidants 6(3), 50 (2017).
  7. Lee, G.-H., Lee, H.-Y., Choi, M.-K., et al. Protective effect of Curcuma longa L. extract on CCl4-induced acute hepatic stress. BMC Res. Notes 10(1), 77 (2017).
  8. Alarifi, S., Ali, H., Alkahtani, S., et al. Regulation of apoptosis through bcl-2/bax proteins expression and DNA damage by nano-sized gadolinium oxide. Int. J. Nanomedicine 12, 4541-4551 (2017).
  9. Velviranli, M., Okudan, N., Revan, S., et al. Repeated supramaximal exercise-induced oxidative stress: Effect of β-alanine plus creatine supplementation. Asian J. Sports Med. 7(1), e26843 (2016).
  10. Yoon, S.P., and Kim, J. Poly(ADP-ribose) polymerase 1 contributes to oxidative stress through downregulation of sirtuin 3 during cisplatin nephrotoxicity. Anat. Cell Biol. 49, 165-176 (2016).
  11. Salvi, A., Patki, G., Khan, E., et al. Relationship between advanced glycation end products and increased lipid peroxidation in semen of diabetic men. Oxid. Med. Cell. Longev. 5059043 (2016).
  12. Kleniewska, P., Hoffmann, A., Pniewska, E., et al. The influence of probiotic Lactobacillus casei in combination with prebiotic inulin on the antioxidant capacity of human plasma. Oxid. Med. Cell. Longev. 1340903, (2016).
  13. Wang, X.X., Edelstein, M.H., Gafter, U., et al. G protein-coupled bile acid receptor TGR5 activation inhibits kidney disease in obesity and diabetes. J. Am. Soc. Nephrol. 27(5), 1362-1378 (2016).
  14. Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of Gluteus Medius muscle in goats. PLOS One 11(5), e0154603 (2016).
  15. Vitti, A., Pellegrini, E., Nali, C., et al. Trichoderma harzianum T-22 induces systemic resistance in tomato infected by Cucumber mosaic virus. Front. Plant Sci. 7(1520), (2016).
  16. Adeyemi, K.D., Shittu, R.M., Sabow, A.B., et al. Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. J. Anim. Sci. Technol. 58(23), (2016).
  17. Dove, R.E., Leong-Smith, P., Roos-Engstrand, E., et al. Cigarette smoke-induced induction of antioxidant enzyme activities in airway leukocytes is absent in active smokers with COPD. Eur. Clin. Respir. J. 2, (2015).
  18. Albaayit, S.F., Abba, Y., Rasdee, A., et al. Effect of Clausena excavata Burm. f. (Rutaceae) leaf extract on wound healing and antioxidant activity in rats. Drug Des. Devel. Ther. 13:9, 3507-3518 (2015).
  19. Rouhollahi, E., Moghadamtousi, S.Z., Hakiaghaalipour, F., et al. Curcuma purpurascens BI. rhizome accelerates rat excisional wound healing: involvement of Hsp70/Bax proteins, antioxidant defense, and angiogenesis activity. Drug Des. Devel. Ther. 9, 5805-5813 (2015).
  20. Karthivashan, G., Arulselvan, P., Alimon, A.R., et al. Competing role of bioactive constituents in Moringa oleifera extract and conventional nutrition feed on the performance of Cobb 500 broilers. Biomed Res. Int. 970398, (2015).
  21. Albaayit, S.F., Abba, Y., Rasedee, A., et al. Effect of Clausena excavata Burm. f. (Rutaceae) leaf extract on wound healing and antioxidant activity in rats. Drug Des. Devel. Ther. 9, 3507-3518 (2015).
  22. Almerich-Silla, J.M., Montiel-Company, J.M., Pastor, S., et al. Oxidative stress parameters in saliva and its association with periodontal disease and types of bacteria. Dis. Markers 653537 (2015).
  23. Christensen, L.L., Selman, C., Blount, J.D., et al. Plasma markers of oxidative stress are uncorrelated in a wild mammal. Ecol. Evol. 5(21), 5096-5108 (2015).
  24. Kadir, F.A., Kassim, N.M., Abdulla, M.A., et al. PASS-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats. Sci. World J. 301879 (2014).
  25. Dhall, S., Do, D.C., Garcia, M., et al. Generating and reversing chronic wounds in diabetic mice by manipulating wound redox parameters. J. Diabetes Res. 562625, (2014).
  26. González-Peña, D., Angulo, J.F., Vallejo, S., et al. High-cholesterol diet enriched with onion affects endothelium-dependent relaxation and NADPH oxidase activity in mesenteric microvessels from Wistar rats. Nutr. Metab. (Lond) 11:57, (2014).
  27. Faisal, M., Saquib, Q., Alatar, A.A., et al. Phytotoxic hazards of NiO-nanoparticles in tomato: A study on mechanism of cell death. J. Hazard. Mater. 250-251, 318-332 (2013).
  28. Guo, C.H., Chen, P.C., Hsu, G.S., et al. Zinc supplementation alters plasma aluminum and selenium status of patients undergoing dialysis: A pilot study. Nutrients 5(4), 1456-1470 (2013).
  29. Eberle, C., Merki, E., Yamashita, T., et al. Maternal immunization affects in utero programming of insulin resistance and type 2 diabetes. PLoS One 7(9), e45361 (2012).
  30. Yuan, C.Y., Lee, Y.J., and Hsu, G.S. Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats. J. Biomed. Sci. 19, 51 (2012).
  31. Liu, I.M., Tzeng, T.F., Liou, S.S., et al. Beneficial effect of traditional chinese medicinal formula danggui-shaoyao-san on advanced glycation end-product-mediated renal injury in streptozotocin-diabetic rats. Evid. Based Complement. Alternat. Med. 2012, 140103 (2012).
  32. Sullivan-Gunn, M.J., Campbell-O'Sullivan, S.P., Tisdale, M.J., et al. Decreased NADPH oxidase expression and antioxidant activity in cachectic skeletal muscle. J. Cachexia Sarcopenia Muscle 2(3), 181-188 (2011).

 

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文章来源:引文数量全球第一,这款SOD检测试剂盒你值得拥有

 

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