原代细胞分离中，针对干细胞涉及到基质细胞血管，羊膜间充质干细胞、脂肪干细胞、产后牙周韧带，滑膜间充质干细胞等等的解离通常会使用酶，如何选择合适的酶来完成对应干细胞的解离，以及原代细胞采集的常规操作， 艾美捷针对干细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
| 干细胞 | |||||
| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 犬/狗 | 23-33 kg | 基质细胞血管 / Stromal vascular | Collagenase: see reference | Media 199E | Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013 |
| 犬/狗 | Canine | 羊膜间充质干细胞 / Amniotic membrane MSC | Trypsin: 0.25%； Collagenase Type 1: 0.2% |
LG-DMEM | Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012 |
| 犬/狗 | Canine, 20-25 kg | 脂肪干细胞 / Adipose stem cells | Collagenase: see reference | Media-199 | Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009 |
| 马 | Equine | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | PBS | Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008 |
| 马 | Equine, 1-5 year | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | PBS | Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007 |
| 人 | Human | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 4: 150-300 u/ml | RPMI-1640 | Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019 |
| 人 | Human | 产后牙周韧带 / Postnatal periodontal ligament | Collagenase Type 1: 0.01% | DMEM/F12 | Yam, G., Teo, E., Setiawan, M., Lovatt, M., Yusoff, N., Fuest, M., Goh, B. and Mehta, J.: Postnatal Periodontal Ligament as a Novel Adult Stem Cell Source for Regenerative Corneal Cell Therapy., J Cell Mol Med , , 2018 |
| 人 | Human | 沃顿果冻间充质基质细胞 / Wharton jelly mesenchymal stromal | Collagenase Type 2: 270 u/ml | DMEM/F12 | Oppliger, B., Joerger-Messerli, M., Simillion, C., Mueller, M., Surbek, D. and Schoeberlein, A.: Mesenchymal Stromal Cells from Umbilical Cord Wharton's Jelly Trigger Oligodendroglial Differentiation in Neural Progenitor Cells Through Cell-to-Cell Contact., Cytotherapy 19, 829-838, 2017 |
| 人 | Human | 脐带间充质基质细胞 / Umbilical cord mesenchymal stromal | Collagenase Type 1: 0.2%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 0.01% |
MEM | de Witte, S., Lambert, E., Merino, A., Strini, T., Douben, H., O'Flynn, L., Elliman, S., de Klein, A., Newsome, P., Baan, C. and Hoogduijn, M.: Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion., Cytotherapy 19, 798-807, 2017 |
| 人 | Human | 滑膜间充质干细胞 / Synovial mesenchymal stem | Collagenase: 0.3% | MEM | Matsumura, E., Tsuji, K., Komori, K., Koga, H., Sekiya, I., Muneta, T.: Pretreatment with IL-1β Enhances Proliferation and Chondrogenic Potential of Synovium-Derived Mesenchymal Stem Cells., Cytotherapy 19, 181-193, 2017 |
| 人 | Human | 脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem | Collagenase animal free: 0.1% | PBS | Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017 |
| 人 | Human | 运动神经元 / Motor neurons | Papain: 20 u/ml | EBSS | Rigamonti, A., Repetti, G., Sun, C., Price, F., Reny, D., Rapino, F., Weisinger, K., Benkler, C., Peterson, Q., Davidow, L., Hansson, E. and Rubin, L.: Large-Scale Production of Mature Neurons from Human Pluripotent Stem Cells in a Three-Dimensional Suspension Culture System., Stem Cell Reports 6, 993-1008, 2016 |
| 人 | Human | 沃顿果冻间充质基质细胞 / Wharton jelly mesenchymal stromal | Collagenase Type 2: 0.6% | DMEM | Lim, J., Razi, Z., Law, J., Nawi, A., Idrus, R. and Ng, M.: MSCs can be Differentially Isolated From Maternal, Middle and Fetal Segments of the Human Umbilical Cord., Cytotherapy 18, 1493-1502, 2016 |
| 人 | Human | 脂肪干细胞 / Adipose stem | Collagenase Type 1: 0.1% | DMEM/F12 | Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016 |
| 人 | Human | 腱干细胞 / Tendon stem | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
MEM | Randelli, P., Menon, A., Ragone, V., Creo P., Alfieri, M., Perucca, O., Banfi, G., Cabitza, P., Tettamanti, G. and Anastasia, L.: Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study., BMC Complement Altern Med 16, 293, 2016 |
| 人 | Human | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 4: 0.2% | PBS | Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015 |
| 人 | Human | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 2: 0.1% | HBSS | Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015 |
| 人 | Human | 牙间充质干细胞 / Dental mesenchymal stem | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM | Wu, Z., Wang, J., Dong, R., Wang, L., Fan, Z., Liu, D. and Wang, S.: Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells., Int J Clin Exp Med 8, 7220-30, 2015 |
| 人 | Human | 蜕膜间充质干细胞 / Decidua mesenchymal stem | Trypsin: 0.25%； Collagenase Type 1: 1.0%； Deoxyribonuclease I: 0.005% |
PBS | Kusuma, G., Menicanin, D., Gronthos, S., Manuelpillai, U., Abumaree, M., Pertile, M., Brennecke, S. and Kalionis, B.: Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua., PLoS ONE 10, e0141246, 2015 |
| 人 | Human | 骨骼肌干细胞 / Skeletal muscle stem | Collagenase Type 2: 750 u/ml； Neutral Protease: 2 u/ml |
Ham's F10 | Charville, G., Cheung, T., Yoo, B., Santos, P., Lee, G., Shrager, J. and Rando, T.: Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells., Stem Cell Reports 5, 621, 2015 |
| 人 | Human | 脊髓神经祖细胞 / Spinal cord neural progenitor | PDS kit: with modifications | EBSS | Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015 |
| 人 | Human | 华顿果冻间充质干细胞 / Wharton jelly mesenchymal stem | Collagenase Type 2: 0.1%； Trypsin NF 1:250: 0.25% |
PBS | Lee, S., Park, B., Kim, J., Jekarl, D., Choi, H., Lee, S., Kim, M., Kim, Y. and Park, M.: The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells., Cytotherapy 17, 1723-31, 2015 |
| 人 | Human, adult | 脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem | Collagenase Type 2: 0.1% | see reference | Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014 |
| 人 | Human, adult | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.15% | DMEM | Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014 |
| 人 | Human | 脐间充质干细胞 / Umbilical mesenchymal stem | Collagenase Type 2: 0.1%； Trypsin: 0.25% |
DMEM | Hang, H., Yu, Y., Wu, N., Huang, Q., Xia, Q. and Bian, J.: Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction., PLoS ONE 9, e104133, 2014 |
| 人 | Human | 心脏干细胞 / Cardiac stem | Collagenase Type 2: 0.25% | Joklik modified Eagle�s | Avolio, E., Gianfranceschi, G., Cesselli, D., Caragnano, A., Athanasakis, E., Katare, R., Meloni, M., Palma, A., Barchiesi, A., Vascotto, C., Toffoletto, B., Mazzega, E., Finato, N., Aresu, G. and Livi, U.: Ex vivo Molecular Rejuvenation Improves the Therapeutic Activity of Senescent Human Cardiac Stem Cells in a Mouse Model of Myocardial Infarction., Stem Cells 32, 2373, 2014 |
| 人 | Human | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 1: 0.1% | PBS | Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods., Cytotherapy 16, 1220-8, 2014 |
| 人 | Human | 皮肤成纤维细胞 / Skin fibroblasts | Collagenase Type 1: 1000 u/ml | DMEM | Douvaras, P., Wang, J., Zimmer, M., Hanchuk, S., O'Bara, M., Sadiq, S., Sim, F., Goldman, J. and Fossati, V.: Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells., Stem Cell Reports 3, 250, 2014 |
| 人 | Human | 脂肪基质细胞干细胞 / Adipose stromal stem | Collagenase animal free: 200 u/ml | DMEM/Hams F-12 | Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells., Tiss Eng 19, 473-8, 2013 |
| 人 | Human, adult | 表皮干细胞 / Epidermal stem cells | Neutral Protease: 2 u/ml； Trypsin: 2.5% |
DMEM | Nagel, S., Rohr, F., Weber, C., Kier, J., Siemers, F., Kruse, C., Danner, S., Brandenburger, M. and Matthiessen, A.: Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro., PLoS ONE 8, e78365, 2013 |
| 人 | Human | 牙髓干细胞 / Dental pulp stem | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM/F12 | Bonnamain, V., Thinard, R., Sergent-Tanguy, S., Huet, P., Bienvenu, G., Naveilhan, P., Farges, J. and Alliot-Licht, B.: Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium., Front Physiol 4, 357, 2013 |
| 人 | Human | 脐带间充质干细胞 / Umbilical cord mesenchymal stem | Collagenase: 0.1-0.2%； Trypsin: 0.1% |
DMEM | Han, Y., Tao, R., Sun, T., Chai, J., Xu, G. and Liu, J.: Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods., Cytotechnology 65, 819, 2013 |
| 人 | Human | 内皮集落形成 / Endothelial colony forming | Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.1%； Neutral Protease: 0.075% |
HBSS | Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2, 839-47, 2013 |
| 人 | Human | 基质细胞血管部分 / Stromal vascular fraction | Collagenase Type 1: 0.075% | PBS | Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis., J Tissue Eng Regen Med 7, 864, 2013 |
| 人 | Human | 肺泡上皮 / Alveolar epithelial | Neutral Protease: 2 u/ml； Trypsin: 0.25%； Elastase: 10 u/ml |
Bronchial Epithelial Growth Medium | Barkauskas, C., Cronce, M., Rackley, C., Bowie, E., Keene, D., Stripp, B., Randell, S., Noble, P. and Hogan, B.: Type 2 Alveolar Cells are Stem Cells in Adult Lung, J Clin Invest 123, 3025, 2013 |
| 人 | Human | 牙髓干细胞 / Dental pulp stem cells | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM | Salmon, B., Bardet, C, Khaddam, M. Naji, J., Coyac, B., Baroukh, B., Letourneur, F., Lesieur, J., Decup, F., Le Denmat, D., Nicoletti, A., Poliard, A, Rowe, P., Huet, E., Vital, S. and Linglart, A.: MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia., PLoS ONE 8, e56749, 2013 |
| 人 | Human | 黑色素瘤 / Melanoma | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.01% |
PBS | Welte, Y., Davies, C., Schafer, R. and Regenbrecht, C.: Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma., J Vis Exp 73, e50200, 2013 |
| 人 | Human | 癌干细胞 / Cancer stem | Trypsin: 0.25%； Collagenase: 0.05%； Hyaluronidase: 0.005% |
RPMI | Dobbin, Z. and Landen, C.: Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications., Curr Protoc Pharmacol 63, Unit 14.28., 2013 |
| 人 | Human | 牙髓来源的干细胞 / Dental pulp derived stem | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM | Sakai, K., Yamamoto, A., Matsubara, K., Nakamura, S., Naruse, M., Yamagata, M., Sakamoto, K., Tauchi, R., Wakao, N., Imagama, S., Hibi, H., Kadomatsu, K., Ishiguro, N. and Ueda, M: Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms., J Clin Invest 122, 80, 2012 |
| 人 | Human | 脂肪来源的干细胞 / Adipose-derived stem | Collagenase Type 1: 0.1% | PBS | Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance., Stem Cells Transl Med 1, 206-20, 2012 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.4%； Deoxyribonuclease I: 0.01% |
DMEM/F12 | Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1, 48, 2012 |
| 人 | Human | 华顿果冻间充质干细胞 / Wharton jelly mesenchymal stem | Collagenase Type 2: 0.1%； Trypsin: 0.25% |
DMEM | Salehinejad, P., Alitheen, N., Ali, A., Omar, A., Mohit, M., Janzamin, E., Samani, F., Torshizi, Z. and Nematollahi-Mahani, S.: Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly., In Vitro Cell Dev Biol Anim 48, 75, 2012 |
| 人 | Human | 胎盘间充质干细胞 / Placental mesenchymal stem | Collagenase Type 2: 0.4%； Deoxyribonuclease I: 0.01% |
DMEM | Vellasamy, S., Sandrasaigaran, P., Vidyadaran, S., George, E. and Ramasamy, R.: Isolation and Characterisation of Mesenchymal Stem cells Derived from Human Placenta Tissue., World J Stem Cells 4, 53-61, 2012 |
| 人 | Human | 脂肪来源的基质细胞血管 / Adipose derived stromal vascular | Collagenase Type 1: 0.1% | PBS | Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery., Stem Cells Transl Med 1, 230-6, 2012 |
| 人 | Human | 血管周围干细胞 / Perivascular stem | Collagenase Type 2: 0.1% | DMEM | James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering., Stem Cells Transl Med 1, 673, 2012 |
| 人 | Human | 绒毛膜间充质 / Chorionic mesenchymal | Collagenase Type 1: 0.1%； Neutral Protease: 2.5 u/ml； Trypsin: 0.25% |
MEM | Nazarov, I., Lee, J., Soupene, E., Etemad, S., Knapik, D., Green, W., Bashkirova, E., Fang, X., Matthay, M., Kuypers, F. and Serikov, V.: Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential., Stem Cells Transl Med 1, 359-72, 2012 |
| 人 | Human, fetal | 肝脏造血 / Liver hematopoietic | Collagenase Type 4: 0.1%； Hyaluronidase: 0.1%； Deoxyribonuclease I: 2 u/ml |
RPMI | Vatakis, D., Bristol, G., Kim, S., Levin, B., Liu, W., Radu, C., Kitchen, S. and Zack, J.: Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model., J Vis Exp 70, e4181, 2012 |
| 人 | Human, male 40-60 years | 脂肪干细胞 / Adipose derived stem cells | Collagenase: 0.25%； Deoxyribonuclease I: 0.002% |
PBS | Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential, Vasc Cell 3, 5, 2011 |
| 人 | Human | 脐带基质细胞干细胞 / Umbilical cord stromal stem | Collagenase Type 4: 0.08%； Neutral Protease: 0.138%； Hyaluronidase: 0.02% |
DMEM | Farias, V., Linares-Fernandez, J., Penalver, J., Paya Colmenero, J., Ferron, G., Duran, E., Fernandez, R., Olivares, E., O'Valle, F., Puertas, A., Oliver, F. and Ruiz de Almodovar, J.: Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties., Placenta 32, 86, 2011 |
| 人 | Human | 血管壁常驻多能干细胞 / Vascular wall-resident multipotent stem cells | Collagenase Type 2: 0.2%； Elastase: 5 u/ml |
see reference | Klein, D., Weisshardt, P., Kleff, V., Jastrow, H., Jakob, H., and Ergun, S.: Vascular Wall-Resident CD44+ Multipotent Stem Cells Vive Rise to Pericytes and Smooth Muscle Cells and Contribute to New Vessel Maturation., PLoS ONE 6, e20540, 2011 |
| 人 | Human | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.1% | DMEM/Ham's F-12 | Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates., Methods Mol Biol 702, 17-27, 2011 |
| 人 | Human | 脂肪来源的间充质 / Adipose derived mesenchymal | Collagenase Type 1: 0.1% | L-DMEM | Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells., J Biomed Sci 18, 59, 2011 |
| 人 | 脑肿瘤 / Brain tumor | Collagenase Type 1: 0.04%；
Hyaluronidase: 0.01%； Deoxyribonuclease I: 0.02%； Neutral Protease: 0.008% |
DMEM/F12 | Hussein, D., Punjaruk, W., Storer, L., Shaw, L., Othman, R., Ottoman, R., Peet, A., Miller, S., Bandopadhyay, G., Heath, R., Kumari, R., Bowman, K., Braker, P., Rahman, R., Jones, G., Watson, S. and Lowe, J.: Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion., Neuro Oncol 13, 70-83, 2011 | |
| 人 | Human | 胶质瘤干细胞 / Glioma stem cells | PDS kit: per instructions | neurobasal medium | Hjelmeland, A., Wu, Q., Wickman, S., Eyler, C., Heddleston, J., Shi, Q., Lathia, J., Macswords, J., Lee, J., McLendon, R. and Rich, J.: Targeting A20 Decreases Glioma Stem Cell Survival and Tumor Growth., PLoS Biol 8, e1000319, 2010 |
| 人 | Human | 牙髓和根尖乳头干细胞 / Dental pulp and apical papilla stem cells | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
MEM | Huang, G., Yamaza, T., Shea, L., Djouad, F., Kuhn, N., Tuan, R. and Shi, S.: Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model., Tissue Eng Part A 16, 605, 2010 |
| 人 | Human | 角膜基质细胞干细胞 / Corneal stromal stem | Neutral Protease: 1.2 u/ml； Collagenase: 0.1% |
DMEM | Du, Y., Roh, D., Funderburgh, M., Mann, M., Marra, K., Rubin, J., Li, X. and Funderburgh, J.: Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro., Mol Vis 16, 2680, 2010 |
| 人 | Human | 脂肪组织来源的干细胞 / Adipose tissue-derived stem | Collagenase Type 2: 1.0% | DMEM/F12 | Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering., J Biomed Mater Res 92, 979, 2010 |
| 人 | Human | 脐带间充质 / Umbilical cord mesenchymal | Hyaluronidase: 0.05%； Collagenase: 0.08% |
DMEM | Zeddou, M., Briquet, A., Relic, B., Josse, C., Malaise, M., Gothot, A., Lechanteur, C. and Beguin, Y.: The Umbilical Cord Matrix is a Better Source of Mesenchymal Stem Cells (MSC) Than the Umbilical Cord Blood., Cell Biol Int 34, 693-701, 2010 |
| 人 | Human | 脂肪干细胞 / Adipose stem | Collagenase Type 1: 0.1% | DMEM | Hareendran, S., Sathishkumar, S., Abbas, S., Mackay, A. and Rajan, P.: A Novel Composition for the Culture of Human Adipose Stem Cells which Includes Complement C3., Cytotechnology 62, 389-402, 2010 |
| 人 | Human | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.1% | DMEM/Hams F-12 | Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes., Cytotherapy 12, 538, 2010 |
| 人 | Human | 结肠上皮 / Colonic epithelial | Collagenase: 150 u/ml； Neutral Protease: 0.04 mg/ml |
Basal X media | Roig, A., Eskiocak, U., Hight, S., Kim, S., Delgado, O., Souza, R., Spechler, S., Wright, W. and Shay, J.: Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro., Gastroenterol 138, 1012, 2010 |
| 人 | Human, 40-65 year | 成人脂肪干细胞 / Adult human adipose stem cells | Collagenase Type 2: 0.075% | DMEM | Sun, N., Panetta, N., Gupta, D., Wilson, K., Lee, A., Jia, F., Hu, S., Cherry, A., Robbins, R., Longaker, M. and Wu, J.: Feeder-Free Derivation of Induced Pluripotent Stem Cells from Adult Human Adipose Stem Cells., Proc Natl Acad Sci U S A 106, 15720, 2009 |
| 人 | Human | 终丝神经祖细胞 / Filum terminale neural progenitor | Trypsin: see reference | DMEM/F-12 | Varghese, M., Olstorn, H., Berg-Johnsen, J., Moe, M., Murrell, W. and Langmoen, I.: Isolation of Human Multipotent Neural Progenitors from Adult Filum Terminale., Stem Cells Dev 18, 603, 2009 |
| 人 | Human | 皮肤和瘢痕疙瘩祖细胞 / Skin and keloid progenitor | Collagenase Type 1: 0.4%； Neutral Protease: 0.3% |
PBS | Zhang, Q., Yamaza, T., Kelly, AP, Shi, S., Wang, S., Brown, J., Wang, L., French, S., Shi, S. and Le, A.: Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis., PLoS ONE 4, e7798, 2009 |
| 人 | Human | 结肠癌 / Colon cancer | Collagenase Type 1: 300 u/ml； Hyaluronidase: 100 u/ml |
DMEM/F12 | Varnat, F., Duquet, A., Malerba, M., Zbinden, M., Mas, C., Gervaz, P. and Ruiz i Altaba, A.: Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion., EMBO Mol Med 1, 338-51, 2009 |
| 人 | Human | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.1% | DMEM | Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function., Stem Cells 27, 230, 2009 |
| 人 | Human | 肿瘤 / Tumor | Collagenase Type 4: 0.1%； Hyaluronidase: 0.07%； Deoxyribonuclease I: 0.04% |
see reference | Sauvageot, C., Weatherbee, J., Kesari, S., Winters, S., Barnes, J., Dellagatta, J., Ramakrishna, N., Stiles, C., Kung, A., Kieran, M. and Wen, P.: Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells.,Neuro Oncol Vol. 11,, , 109, 2009 |
| 人 | Human | 结肠上皮 / Colonic epithelial | Collagenase Type 4: 0.1% | not listed | Huang, E., Hynes, M., Zhang, T., Ginestier, C., Dontu, G., Appelman, H., Fields, J., Wicha, M. and Boman, B.: Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis., Cancer Res 69, 3382-9, 2009 |
| 人 | Human, male | 精原干细胞 / Spermatogonial stem cells | Collagenase: 1%； Deoxyribonuclease I: 0.22%； Trypsin: 0.4% |
DMEM | Kossack, N., Meneses, J., Shefi, S., Nguyen, H., Chavez, S., Nicholas, C., Gromoll, J., Turek, P. and Reijo-Pera, R.: Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells., Stem Cells , , 2008 |
| 人 | Human | 肌肉来源的的多祖细胞 / Muscle derived multiprogenitor cells | Collagenase Type 2: 0.05% | DMEM | Nesti, L., Jackson, W., Shanti, R., Koehler, S., Aragon, A., Bailey, J., Sracic, M., Freedman, B., Giuliani, J. and Tuan, R.: Differentiation Potential of Multipotent Progenitor Cells Derived from War-Traumatized Muscle Tissue., J Bone Joint Surg Am 90, 2390, 2008 |
| 人 | Human | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.075% | DMEM | Jeong, J.: Adipose Stem Cells as a Clinically Available and Effective Source of Adult Stem Cell Therapy, Int J Stem Cells 1, 43, 2008 |
| 人 | Human | 肝干细胞和成肝细胞 / Hepatic stem cells and heptoblasts | Collagenase Type 4: 0.014-0.06% | various | Wauthier, E., Schmelzer, E., Turner, W., Zhang, L., LeCluyse, E., Ruiz, J., Turner, R., Furth, M., Kubota, H., Lozoya, O., Barbier, C., McClelland, R., Yao, H., Moss, N., Bruce, A., Ludlow, J. and Reid, L.: Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance., Methods Cell Biol 86, 137, 2008 |
| 人 | Human | 脐间充质干细胞 / Umbilical mesenchymal stem | Collagenase: 0.1% | DMEM | Secco, M., Zucconi, E., Vieira, N., Fogaca, L.,Cerqueira, A., Carvalho, M., Jazedje, T., Okamoto, O., Muotri, A. and Zatz, M.: Multipotent Stem Cells from Umbilical Cord: Cord is Richer Than Blood!, Stem Cells 26, 146, 2008 |
| 人 | Human | 干细胞 / Stem | Collagenase: 280 u/ml | D-PBS | Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells., Regen Med 3, 705-15, 2008 |
| 人 | Human | 骨髓来源的MSC / Bone marrow derived MSC | Trypsin: 0.05%； Papain: 0.0025% |
DMEM | Welter, J., Solchaga, L.and Penick, K.: Simplification of Aggregate Culture of Human Mesenchymal Stem Cells as a Chondrogenic Screening Assay, Biotechniques 42, 732, 2007 |
| 人 | Human, 8-12 year | 腱干细胞/祖细胞 / Tendon stem/progenitor | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM | Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13, 1219-27, 2007 |
| 人 | Human, male | 脂肪来源的成体干细胞 / Adipose derived adult stem cells | Collagenase Type 1: 0.1% | DMEM | Lei, L., Liao, W., Sheng, P., Fu, M., He, A. and Huang, G.: Biological Character of Human Adipose-Derived Adult Stem Cells and Influence of Donor Age on Cell Replication in Culture., Sci China C Life Sci 50, 320, 2007 |
| 人 | Human | 胰腺癌干细胞 / Pancreatic cancer stem cells | Collagenase Type 4: 200 u/ml | medium 199 | Li, C., Heidt, D., Dalerba, P., Burant, C., Zhang, L., Adsay, V., Wicha, M., Clarke, M. and Simeone, D.: Identification of Pancreatic Cancer Stem Cells., Cancer Res 67, 1030, 2007 |
| 人 | Human | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | DMEM/F12 | Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors., J Cell Physiol 212, 702-9, 2007 |
| 人 | Human | 口腔上皮 / Oral epithelial | Neutral Protease: 1.2 u/ml； Trypsin: 0.05% |
PBS | Nakamura, T., Endo, K. and Kinoshita, S.: Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling., Stem Cells 25, 628-38, 2007 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 2: 10%； Neutral Protease: see reference |
CMF-DPBS | Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol Chapter 1, Unit 1E.2, 2007 |
| 人 | Human, adult | 脂肪来源的成体干细胞 / Adipose-derived adult stem | Collagenase Type 1: 0.1% | DMEM/F-12 Ham's | Mitchell James B, McIntosh Kevin, Zvonic Sanjin, Garrett Sara, Floyd Z Elizabeth, Kloster Amy, Di Halvorsen Yuan, Storms Robert W, Goh Brian, Kilroy Gail, Wu Xiying, Gimble Jeffrey M: Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers, Stem Cells 24, 376-85, 2006 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.075% | DMEM | Kern Susanne, Eichler Hermann, Stoeve Johannes, Kluter Harald, Bieback Karen: Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue, Stem Cells 24, 1294-301, 2006 |
| 人 | Human | 胎盘间充质干细胞 / Placental mesenchymal stem | Collagenase Type 2: 270 u/ml； Neutral Protease: 2.4 u/ml |
MEM | Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV: Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration, Am J Obstet Gynecol 194, 664-73, 2006 |
| 人 | Human | 基质细胞干细胞 / Stromal stem cells | Collagenase Type 1: 0.2% | HBSS | Boquest Andrew C, Shahdadfar Aboulghassem, Brinchmann Jan E, Collas Philippe: Isolation of stromal stem cells from human adipose tissue, Methods Mol Biol 325, 35-46, 2006 |
| 人 | Human, female | 脂肪来源的成体干细胞 / Adipose derived adult stem cells | Collagenase Type 1: 0.1% | DMEM/F-12 | Guilak, F., Lott, K., Awad, H., Cao, Q., Hicok, K., Fermor, B. and Gimble, J.: Clonal Analysis of the Differentiation Potential of Human Adipose-Derived Adult Stem Cells., J Cell Physiol 206, 229, 2006 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.1% | HBSS | Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47, 653-64, 2006 |
| 人 | Human | 毛囊上皮 / Hair follicular epithelial | Neutral Protease: 1.25%； Trypsin: 0.25% |
DMEM/F-12 | Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei: Isolation of a novel population of multipotent adult stem cells from human hair follicles, Am J Pathol 168, 1879-88, 2006 |
| 人 | Human | 成体干细胞 / Adult stem cells | Collagenase Type 1: 0.1% | PBS | Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005 |
| 人 | Human | 脂肪细胞，基质细胞血管 / Adipocytes, stromal vascular | Collagenase: 0.2% | HBSS | Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell 16, 1131-41, 2005 |
| 人 | Human, female | 脂肪来源的成体干细胞 / Adipose derived adult stem cells | Collagenase Type 1: 0.1% | DMEM-Ham's F-12 | Aust, L., Devlin, B., Foster, S., Halvorsen, Y., Hicok, K., du Laney, T., Sen, A., Willingmyre, G. and Gimble, J.: Yield of Human Adipose-Derived Adult Stem Cells from Liposuction Aspirates., Cytometry 6, 7-14, 2004 |
| 人 | Human | 基质细胞血管，脂肪细胞，干细胞 / Stromal vascular, adipocytes, stem | Collagenase: 300 u/ml | PBS | Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004 |
| 人 | Human, neonatal | 角质形成细胞 / Keratinocytes | Neutral Protease: 0.4%； Collagenase: 0.3% |
DMEM | Li, A., Pouliot, N., Redvers, R., and Kaur, P.: Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny, J Clin Invest 113, 390-400, 2004 |
| 人 | Human, adult, male | 人表皮角质形成细胞干细胞 / Human epidermal keratinocyte stem cells | Neutral Protease: 0.5% | DMEM | Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.: Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture, Stem Cells 21, 481, 2003 |
| 人 | Human | 脐静脉间充质干细胞 / Umbilical vein mesenchymal stem cells | Collagenase: 1% | PBS | Covas DT, Siufi JL C, Silva AR L, Orellana MD: Isolation and culture of umbilical vein mesenchymal stem cells, Braz J Med Biol Res 36, 1179-83, 2003 |
| 人 | Human, 7-8 yr | 干细胞 人类脱落的乳牙 / Stem cells Human exfoliated deciduous teeth | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
see reference | Miura, M., Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P, and Shi, S.: SHED: Stem Cells from Human Exfoliated Deciduous Teeth, Proc Natl Acad Sci U S A 100, 5807, 2003 |
| 人 | Human, fetal | 上皮祖细胞 / Epithelial progenitor | Collagenase: 0.03% | DMEM | Malhi, H., Irani, A., Gagandeep, S. and Gupta, S.: Isolation of Human Progenitor Liver Epithelial Cells with Extensive Replication Capacity and Differentiation into Mature Hepatocytes., J Cell Sci 115, 2679, 2002 |
| 人 | Human | 处理过的脂肪抽吸细胞 / Processed lipoaspirate cells | Collagenase Type 1: 0.075% | PBS | Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell 13, 4279-95, 2002 |
| 人 | Human, adult | 人皮肤肥大细胞 / Human skin mast cells | Collagenase Type 2: 0.15%； Hyaluronidase: 0.07%； Deoxyribonuclease I: 0.03% |
HBSS | Kambe, N, Kambe, M, Kochan, J, and Schwartz ,L.: Human Skin-derived Mast Cells Can Proliferate While Retaining Their Characteristic Functional and Protease Phenotypes, Blood 97, 2045, 2001 |
| 人 | Human | 干细胞，胚 / Stem, embryonic | Neutral Protease: 0.01% - 0.02% | DMEM | Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells, Nat Biotechnol 19, 1129, 2001 |
| 人 | Human | 中枢神经系统干细胞 / Central nervous system stem | Collagenase: 0.1%； Hyaluronidase: 0.1% |
HBSS | Uchida, N., Buck, D., He, D., Reitsma, M., Masek, M., Phan, T., Tsukamoto, A., Gage, F. and Weissman, I.: Direct Isolation of Human Central Nervous System Stem Cells., Proc Natl Acad Sci U S A 97, 14720, 2000 |
| 人 | Human | 胚干细胞 / Embryonic stem | Neutral Protease: 1%； Collagenase Type 4: 0.1% |
DMEM | Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts, Science 282, 1145-7, 1998 |
| 人 | Human | 肌肉来源的干细胞 / Muscle-derived stem cells | Trypsin: 0.25% | DMEM/F12 | Alessandri Giulio, Pagano Stefano, Bez Alessandra, Benetti Anna, Pozzi Stefano, Iannolo Gioacchin, Baronio Manuela, Invernici Gloria, Caruso Arnaldo, Muneretto Claudio, Bisleri Gianluigi, Parati Eugenio: Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages, Lancet 364, 1872-83, |
| 昆虫 | Drosophila, larval | 神经母细胞 / Neuroblasts | Collagenase Type 1: 0.1%； Papain: 0.1% |
Rinaldini solution | Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal., Cell Rep 2, 407, 2012 |
| 猴 | Monkey | 胚干细胞 / Embryonic stem | Collagenase Type 4: 0.08% | DMEM | Chen, S., Revoltella, R., Papini, S., Michelini, M., Fitzgerald, W., Zimmerberg, J.,and Margolis, L.: Multilineage Differentiation of Rhesus Monkey Embryonic Stem cells in Three-dimensional Culture Systems, Stem Cells 21(3), 281, 2003 |
| 猴 | Baboons, 1yr, 7yr, 14 yr | 灵长类精原细胞 / Primate spermatogonial | Collagenase Type 2: 0.1%； Trypsin: 0.05%； Deoxyribonuclease I: 0.1% |
DMEM | Nagano, M., McCarrey, J., and Brinster, R.: Primate Spermatogonial Stem Cells Colonize Mouse Testes, Biol Reprod 64, 1409, 2001 |
| 小鼠 | Mouse, xenograft | 胰腺癌 / Pancreatic cancer | Stemxyme 1: 0.2% | HBSS | Pham, K., Delitto, D., Knowlton, A., Hartlage, E., Madhavan, R., Gonzalo, D., Thomas, R., Behrns, K., George, T., Hughes, S., Wallet, S., Liu, C., Trevino, J.: Isolation of Pancreatic Cancer Cells from a Patient-Derived Xenograft Model Allows for Practical Expansion and Preserved Heterogeneity in Culture., Am J Pathol 186, , 2016 |
| 小鼠 | Mouse, GFP transgenic | 肌肉来源的的 NLSC / Muscle derived NLSC | Collagenase Type 2: 0.2% | DMEM | Birbrair, A, Sattiraju, A, Zhu, D, Zulato, G, Batista, I, Nguyen, V, Messi M, Solingapuram Sai, K, Marini, F, Delbono, O and Mintz, A.: Novel Peripherally Derived Neural-Like Stem Cells as Therapeutic Carriers for Treating Glioblastomas., Stem Cells Transl Med , , 2016 |
| 小鼠 | Mouse | 脂肪间质基质细胞 / Adipose mesenchyal stromal | Collagenase Type 2: 0.2% | DMEM | Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis., Cytotherapy 18, 1129-45, 2016 |
| 小鼠 | Mouse, 3-4 week | 心脏祖细胞 / Cardiac progenitor | Collagenase Type 1: 0.1% | DMEM | Wang, H., Chen, H., Feng, B., Wang, X., He, X., Hu, R., Yin, M., Wang, W., Fu, W. and Xu, Z.: Isolation and Characterization of a Sca-1+/CD31- Progenitor Cell Lineage Derived From Mouse Heart Tissue., BMC Biotechnol 14, 75, 2014 |
| 小鼠 | Mouse | 上皮干细胞 / Epithelial stem | Collagenase: 2% | DMEM/F12 | Chavez, M., Hu, J., Seidel, K., Li, C., Jheon, A., Naveau, A., Horst, O. and Klein, O.: Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor., J Vis Exp , , 2014 |
| 小鼠 | Mouse, P4 | 肠神经嵴祖细胞 / Enteric neural crest progenitors | Neutral Protease: 0.5%； Collagenase animal free: 0.05% |
DMEM/F12 | Hotta, R., Stamp, L., Foong, J., McConnell, S., Bergner, A., Anderson, R., Enomoto, H., Newgreen, D., Obermayr, F., Furness, J. and Young, H.: Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon., J Clin Invest 123, 1182, 2013 |
| 小鼠 | Mouse | 上皮干细胞 / Epithelial stem | Trypsin: 0.25%； Collagenase Type 1: 0.4% |
HBSS | Jensen, U., Ghazizadeh, S. and Owens, D.: Isolation and Characterization of Cutaneous Epithelial Stem Cells., Methods Mol Biol 989, 61-9, 2013 |
| 小鼠 | Mouse | 结肠肿瘤类器官 / Colon tumor organoids | Collagenase Type 4: 200 u/ml； Neutral Protease: 0.01% |
DMEM | Xue, X. and Shah, Y.: In Vitro Organoid Culture of Primary Mouse Colon Tumors., J Vis Exp , e50210, 2013 |
| 小鼠 | Mouse, 8-12 week | 胰腺类器官 / Pancreas organoid | Collagenase: 0.012%； Neutral Protease: 0.012% |
DMEM | Huch, M., Bonfanti, P., Boj, S., Sato, T., Loomans, C., Van de Wetering, M., Sojoodi, M., Li, V., Schuijers, J., Gracanin, A., Ringnalda, F., Begthel, H., Hamer, K., Mulder, J., Van Es, J. and De Koning, E.: Unlimited In Vitro Expansion of Adult Bi-Potent Pancreas Progenitors Through the Lgr5/R-Spondin Axis., EMBO J 32, 2708-21, 2013 |
| 小鼠 | Mouse | 乳腺肿瘤 / Mammary tumor | Collagenase: 0.15%； Hyaluronidase: 0.020% |
DMEM/F12 | Liu, X., Johnson, S., Liu, S., Kanojia, D., Yue, W., Singh, U., Wang, Q, Wang Qi, Nie, Q. and Chen H.: Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy., Sci Rep 3, 2473, 2013 |
| 小鼠 | Mouse, 4-6 week | 雪旺 / Schwann | Collagenase Type 1: 0.01%； Trypsin: 0.125% |
DMEM | Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection., Cell 152, 51, 2013 |
| 小鼠 | Mouse, adult | 精原干细胞 / Spermatogonial stem | Trypsin: 0.05%； Collagenase Type 1: 0.03%； Deoxyribonuclease I: 80 u/ml |
DMEM | Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines., J Vis Exp 72, e50017, 2013 |
| 小鼠 | Mouse | 前列腺肿瘤 / Prostate tumor | Collagenase Type 4: 1,600 u/ml | DMEM/F12 | Mazzoleni, S., Jachetti, E., Morosini, S., Grioni, M., Piras, I., Pala, M., Bulfone, A., Freschi, M., Bellone, M. and Galli, R.: Gene Signatures Distinguish Stage-Specific Prostate Cancer Stem Cells Isolated From Transgenic Adenocarcinoma of the Mouse Prostate Lesions and Predict the Malignancy of Human Tumors., Stem Cells Transl Med 2, 678, 2013 |
| 小鼠 | Mouse, 10 week | 滑膜间充质 / Synovial mesenchymal | Collagenase: 0.1%； Deoxyribonuclease I: 0.005% |
DMEM | Futami, I., Ishijima, M., Kaneko, H., Tsuji, K., Ichikawa-Tomikawa, N., Sadatsuki, R., Muneta, T., Arikawa-Hirasawa, E., Sekiya, I. and Kaneko, K.: Isolation and Characterization of Multipotential Mesenchymal Cells from the Mouse Synovium., PLoS ONE 7, e45517, 2012 |
| 小鼠 | Mouse, 6-8 week | 神经干细胞 / Neural stem cells | Papain: 0.01%； Neutral Protease: 0.1%； Deoxyribonuclease I: 0.01% |
DMEM/F12 | Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis., J Neurosci 32, 3376-87, 2012 |
| 小鼠 | Mouse | 肠道类器官 / Intestinal organoids | Collagenase Type 1: 800 u/ml； Neutral Protease: 0.013% |
DMEM | Barthel, E., Speer, A., Levin, D., Sala, F., Hou, X., Torashima, Y., Wigfall, C. and Grikscheit, T.: Tissue Engineering of the Intestine in a Murine Model., J Vis Exp 70, e4279, 2012 |
| 小鼠 | Mouse | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 2: 0.1% | DMEM | Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair., Stem Cells Transl Med 1, 803, 2012 |
| 小鼠 | Mouse | 肺肿瘤 / Lung tumor | Neutral Protease: 50 u/ml； Collagenase: 400 u/ml； Deoxyribonuclease I: 50 u/ml |
DMEM | Vaughan, A., Halbert, C., Wootton, S. and Miller, A.: Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation., Mol Cancer Res 10, 86, 2012 |
| 小鼠 | Mouse, P4-5 | 神经鞘 / Neuroshere | Trypsin: 0.25%； Papain: 100 u； Deoxyribonuclease I: 0.025% |
Pro-N | Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors., Stem Cells 30, 1265, 2012 |
| 小鼠 | Mouse | 神经祖细胞 / Nerve progenitors | Collagenase Type 4: 0.025%； Trypsin NF 1:250: .025% |
HBSS | Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2., Stem Cells Transl Med 1, 874-85, 2012 |
| 小鼠 | Mouse, 8-10 week | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.025% | HBSS | Sugii, S., Kida, Y., Berggren, W. and Evans, R.: Feeder-Dependent and Feeder-Independent iPS Cell Derivation from Human and Mouse Adipose Stem Cells., Nat Protoc 6, 346, 2011 |
| 小鼠 | Mouse | 脾脏，骨髓内皮 / Spleen, bone marrow endothelial | Collagenase Type 4: 0.3-1.0%； Deoxyribonuclease I: 20 u/ml |
PBS | Shi, C., Jia, T., Mendez-Ferrer, S., Hohl, T., Serbina, N., Lipuma, L., Leiner, I., Li, M., Frenette, P. and Pamer, E.: Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands., Immunity 34, 590, 2011 |
| 小鼠 | Mouse, 8-10 week | 肺间充质干细胞 / Lung mesenchymal stem | Collagenase Type 2: 0.2% | HBSS | Chow, K., Jun, D., Helm, K., Wagner, D. and Majka, S.: Isolation & Characterization of Hoechst(low) CD45(negative) Mouse Lung Mesenchymal Stem Cells., J Vis Exp 56, e3159, 2011 |
| 小鼠 | Mouse, neonatal | 牙髓干细胞 / Dental pulp stem | Collagenase Type 4: 0.2%； Neutral Protease: 1.2 u/ml |
PBS | Janebodin, K., Horst, O., Ieronimakis, N., Balasundaram, G., Reesukumal, K., Pratumvinit, B. and Reyes, M.: Isolation and Characterization of Neural Crest-Derived Stem Cells from Dental Pulp of Neonatal Mice., PLoS ONE 6, e27526, 2011 |
| 小鼠 | Mouse | CD133+ 干细胞 / CD133+ stem | Collagenase Type 4: 0.05%； Pronase: 0.05%； Deoxyribonuclease I: 0.01% |
DMEM/F12 | Rountree, C., Ding, W., Dang, H., Vankirk, C. and Crooks, G.: Isolation of CD133+ Liver Stem Cells for Clonal Expansion., J Vis Exp 56, e3183, 2011 |
| 小鼠 | Mouse | 干细胞和祖 / Stem and progenitor | Collagenase Type 2: 0.2% | HBSS | Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010 |
| 小鼠 | Mouse, 6-8 week | 骨髓间充质干细胞 / Bone marrow mesenchymal stem | Collagenase Type 1: 0.25% | RPMI 1640 | Xu, S., De Becker, A., Van Camp, B., Vanderkerken, K. and Van Riet, I.: An Improved Harvest and In Vitro Expansion Protocol for Murine Bone Marrow-Derived Mesenchymal Stem Cells., J Biomed Biotechnol 2010, 105940, 2010 |
| 小鼠 | Mouse | 骨内膜细胞 / Endosteal cells | Collagenase Type 1: 0.3% | DMEM | Nakamura, Y., Arai, F., Iwasaki, H., Hosokawa, K., Kobayashi, I., Gomei, Y., Matsumoto, Y., Yoshihara, H. and Suda, T.: Isolation and Characterization of Endosteal Niche Cell Populations that Regulate Hematopoietic Stem Cells., Blood 116, 1422, 2010 |
| 小鼠 | Mouse, 10 week | 胰岛 / Islets | Collagenase: 0.2% | RPMI 1640 | Kobayashi, T., Yamaguchi, T., Hamanaka, S., Kato-Itoh, M., Yamazaki, Y., Ibata, M., Sato, H., Lee, Y., Usui, J., Knisely, A., Hirabayashi, M. and Nakauchi, H.: Generation of Rat Pancreas in Mouse by Interspecific Blastocyst Injection of Pluripotent Stem Cells., Cell 142, 787, 2010 |
| 小鼠 | Mouse | 胰腺癌干细胞 / Pancreatic cancer stem | Collagenase Type 4: 200 u/ml； Neutral Protease: 0.6 u/ml |
DMEM | Rasheed, Z., Wang, Q. and Matsui, W.: Isolation of Stem Cells from Human Pancreatic Cancer Xenografts., J Vis Exp 43, 2169, 2010 |
| 小鼠 | Mouse, adult | 成体神经干细胞 / Adult neural stem | Trypsin: 0.05% | DMEM | Deleyrolle, L. and Reynolds, B.: Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells using the Neurosphere Assay., Methods Mol Biol 549, 91, 2009 |
| 小鼠 | Mouse, 6-8 week | 前列腺上皮/干细胞 / Prostate epithelial/stem | Collagenase Type 2: 0.5%； Trypsin: 0.05% |
HBSS | Burger, P., Gupta, R., Xiong, X., Ontiveros, C., Salm, S., Moscatelli, D. and Wilson, E.: High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells., Stem Cells 27, 2220-8, 2009 |
| 小鼠 | Mouse | 脂肪组织来源的干细胞 / Adipose tissue-derived stem | Collagenase Type 1: 0.075% | Modified Eagles | Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model., Stem Cells 27, 259-65, 2009 |
| 小鼠 | Mouse | 骨髓 / Bone marrow | Collagenase: 0.2% | DMEM | Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H. and Matsuzaki, Y.: Prospective Identification, Isolation, and Systemic Transplantation of Multipotent Mesenchymal Stem Cells in Murine Bone Marrow., J Exp Med 206, 2483-96, 2009 |
| 小鼠 | Mouse | 生殖细胞 / Germ cells | Collagenase Type 1: 100 u/ml | HBSS | Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells., Proc Natl Acad Sci U S A 105, 10420, 2008 |
| 小鼠 | Mouse | 神经祖细胞 / Neural progenitor cell | Papain: 10 ul/ml | PBS | Hutton, S. and Pevny, L.: Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System., Cold Spring Harb. Protoc. 11, 5077, 2008 |
| 小鼠 | Mouse, 3-6 week | 耳间充质干细胞 / Ear mesenchymal stem | Collagenase Type 1: 0.2% | DMEM/F12 | Staszkiewicz, J., Gimble, J., Manuel, J. and Gawronska-Kozak, B.: IFATS Collection: Stem Cell Antigen-1-Positive Ear Mesenchymal Stem Cells Display Enhanced Adipogenic Potential., Stem Cells 26, 2666, 2008 |
| 小鼠 | Mouse, postnatal | 星形胶质细胞 / Astrocytes | Trypsin: 0.25%； Deoxyribonuclease I: 1,000 u/ml |
HBSS | Sher, F., Rossler, R., Brouwer, N., Balasubramaniyan, V., Boddeke, E. and Copray, S.: Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2., Stem Cells 26, 2875, 2008 |
| 小鼠 | Mouse, 12 week | 乳腺上皮 / Mammary epithelial | Collagenase: 0.3%；
Hyaluronidase: 100 u/ml； Trypsin: 0.25%； Neutral Protease: 0.5%； Deoxyribonuclease I: 0.01% |
see reference | Taddei, I., Deugnier, M., Faraldo, M., Petit, V., Bouvard, D., Medina, D., Fassler, R., Thiery, J. and Glukhova, M.: Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells, Nat Cell Biol 10, 716-22, 2008 |
| 小鼠 | Mouse, embryonic | 造血干细胞 / Hematopoietic stem cells | Collagenase: 0.1% | PBS | Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta., J Vis Exp 16, e742, 2008 |
| 小鼠 | Mouse, embryonic | HES-BC细胞 / HES-BC cells | Trypsin: 0.05% | DMEM | Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert: Generation of functional hemangioblasts from human embryonic stem cells, Nat Methods 4, 501-9, 2007 |
| 小鼠 | Mouse, 6-8 week | 腱干细胞/祖细胞 / Tendon stem/progenitor | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM | Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F: Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche, Nat Med 13, 1219-27, 2007 |
| 小鼠 | Mouse | 肿瘤 / Tumor | Collagenase Type 3: 200 u/ml | RPMI-1640 | Prince, M., Sivanandan, R., Kaczorowski, A., Wolf, G., Kaplan, M., Dalerba, P., Weissman, I., Clarke, M. and Ailles, L.: Identification of a Subpopulation of Cells with Cancer Stem Cell Properties in Head and Neck Squamous Cell Carcinoma., Proc Natl Acad Sci U S A 104, 973, 2007 |
| 小鼠 | Mouse | 肝上皮祖细胞 / Liver epithelial progenitor cells | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.05% |
DMEM | Li Wen-Lin, Su Juan, Yao Yu-Cheng, Tao Xin-Rong, Yan Yong-Bi, Yu Hong-Yu, Wang Xin-Min, Li Jian-Xiu, Yang Yong-Ji, Lau Joseph T Y, Hu Yi-Ping: Isolation and characterization of bipotent liver progenitor cells from adult mouse, Stem Cells 24, 322-32, 2006 |
| 小鼠 | Mouse, 6 week | 脂肪间充质干细胞 / Adipose mesenchymal stem | Collagenase: 0.2% | PBS | Di Rocco Giuliana, Iachininoto Maria Grazia, Tritarelli Alessandra, Straino Stefania, Zacheo Antonella, Germani Antonia, Crea Filippo, Capogrossi Maurizio C: Myogenic potential of adipose-tissue-derived cells, J Cell Sci 119, 2945-52, 2006 |
| 小鼠 | Mouse | 神经干细胞 / Neural stem cells | Papain: see reference | DMEM/F-12 | Meletis, K., Wirta, V., Hede, S., Nister, M., Lundeberg, J. abd Frisen, J.: p53 Suppresses the Self-Renewal of Adult Neural Stem Cells., Development 133, 363, 2006 |
| 小鼠 | Mouse, 10-12 week | 肾 / Kidney | Collagenase Type 4: 0.075% | DMEM | Dekel, B., Zangi, L., Shezen, E., Reich-Zeliger, S., Eventov-Friedman, S., Katchman, H., Jacob-Hirsch, J., Amariglio, N., Rechavi, G., Margalit, R. and Reisner, Y.: Isolation and Characterization of Nontubular sca-1+lin- Multipotent Stem/Progenitor Cells from Adult Mouse Kidney., J Am Soc Nephrol 17, 3300, 2006 |
| 小鼠 | Mouse, day 7 | 小脑干细胞 / Cerebellar stem cells | Papain: 10u/ml； Deoxyribonuclease I: 250 u/ml |
Dulbecco's PBS | Lee, A, Kessler, J, Read, T, Kaiser, C, Corbeil, D, Huttner ,W, Johnson, J, Wechsler-Reya, R.: Isolation of Neural Stem Cells from the Postnatal Cerebellum, Nat Neurosci 8, 723, 2005 |
| 小鼠 | Mouse, embryonic | 胚胎成纤维细胞饲养细胞 / Embryonic fibroblast feeder cells | Collagenase Type 4: 0.1% | DMEM | Schatten Gerald, Smith Joseph, Navara Christopher, Park Jong-Hyuk, Pedersen Roger: Culture of human embryonic stem cells, Nat Methods 2, 455-63, 2005 |
| 小鼠 | Mouse, 1 day | 神经祖细胞 / Neural progenitor | PDS kit: per instructions | see reference | Seaberg, R., Smukler, S. and Van der Kooy, D.: Intrinsic Differences Distinguish Transiently Neurogenic Progenitors from Neural Stem Cells in the Early Postnatal Brain., Dev Biol 278, 71, 2005 |
| 小鼠 | Mouse, 8-10 week | 肝源性干细胞 / Liver derived stem cells | Collagenase Type 1: 0.1%； Neutral Protease: 2.4 u/ml |
HBSS | Kotton, D., Fabian, A. and Mulligan, R.: A Novel Stem-Cell Population in Adult Liver with Potent Hematopoietic-Reconstitution Activity., Blood 106, 1574, 2005 |
| 小鼠 | Mouse | 神经球 / Neurosperes | PDS kit: per instructions | DMEM/F12 | Klein C, Butt SJ, Machold RP, Johnson JE, and Fishell G.: Cerebellum- and forebrain-derived stem cells possess intrinsic regional character, Development 132, 4497, 2005 |
| 小鼠 | Mouse | 骨髓 / Bone marrow | Collagenase Type 1: 0.15%； Neutral Protease: 0.15% |
PBS | Bertoncello, I. and Williams, B.: Hematopoietic Stem Cell Characterization by Hoechst 33342 and Rhodamine 123 Staining., Methods Mol Biol 263, 181, 2004 |
| 小鼠 | Mouse, 4-7 day | 多能干细胞 / Pluripotent stem cells | Collagenase Type 1: 220 u/ml； Neutral Protease: 33 u/ml |
MEM | Howell, J., Lee, W., Morrison, P., Zhong, J., Yoder, M. and Srour, E.: Pluripotent Stem Cells Identified in Multiple Murine Tissues., Ann N Y Acad Sci 996, 158, 2003 |
| 小鼠 | Mouse, 15 day or 3 month | 神经干细胞 / Neural stem cells | Papain: 0.1% | DMEM/F-12 | Gritti, A, Bonfanti, L, Doetsch, F, Caille, I, Alvarez-Buylla, A, Lim, D, Galli, R, Verdugo J, Herrera, D, and Vescovi A.: Multipotent Neural Stem Cells Reside into the Rostral Extension and Olfactory Bulb of Adult Rodents, J Neurosci 22(2), 437, 2002 |
| 小鼠 | Mouse, embryonic | 皮质祖细胞 / Cortical progenitors | PDS kit: per instructions | Serum free medium | Estivill-Torrus, G., Pearson, H., Van Heyningen, V., Price, D. and Rashbass, P.: Pax6 is Required to Regulate the Cell Cycle and the Rate of Progression from Symmetrical to Asymmetrical Division in Mammalian Cortical Progenitors., Development 129, 455, 2002 |
| 小鼠 | Mouse, 6-8 week | 肌细胞 / Myocytes | Collagenase Type 2: 0.2%； Trypsin: 0.25% |
HBSS | McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.: Muscle-derived hematopoietic stem cells are hematopoietic in origin, Proc Natl Acad Sci U S A 99, 1341, 2002 |
| 小鼠 | Mouse | 乳腺上皮干细胞 / Mammary epithelial stem | Collagenase Type 3: 0.1%； Hyaluronidase: 0.1%； Pronase: 1.25%； Deoxyribonuclease I: 0.2% |
DMEM | Boulanger CA, Smith GH: Reducing mammary cancer risk through premature stem cell senescence, Oncogene 20, 2264-72, 2001 |
| 小鼠 | Mouse, male | 前列腺干细胞 / Prostatic stem | Collagenase Type 1: 170 u/ml | DMEM | Dubey P, Wu H, Reiter RE, Witte ON: Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression, Cancer Res 61, 3256-61, 2001 |
| 小鼠 | Mouse, 4-8 month | 神经室下区 / Neural subventricular zone | Trypsin: 0.13%； Hyaluronidase: 0.067% |
DMEM/F12 | Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C, and Vescovi A.: Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain, J Neurosci 19(9), 3287, 1999 |
| 小鼠 | Mouse, 6 week | 肌肉造血干细胞 / Muscle hematopoietic stem cells | Collagenase: 0.2%； Trypsin: 0.1% |
DMEM | Jackson, K., and Goodell, M.: Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle, Proc Natl Acad Sci U S A 96, 14482, 1999 |
| 绵羊 | Ovine, 3-5 year | 子宫内膜间质 / Endometrial stromal | Collagenase Type 1: 0.5%； Deoxyribonuclease I: 0.04 u/ml |
DMEM/F-12 | Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium., PLoS ONE 10, e0127531, 2015 |
| 猪 | Porcine, 3-4 month | 滑膜和皮肤干细胞 / Synovial membrane and skin stem | Collagenase: 0.2% | DMEM | Ando, W., Kutcher, J., Krawetz, R., Sen, A., Nakamura, N., Frank, C. and Hart, D.: Clonal Analysis of Synovial Fluid Stem Cells to Characterize and Identify Stable Mesenchymal Stromal Cell/Mesenchymal Progenitor Cell Phenotypes in a Porcine Model: A Cell Source with Enhanced Commitment to the Chondrogenic Lineage., Cytotherapy 16, 776, 2014 |
| 猪 | Porcine, 1-4 day | 睾丸 / Testicular | Collagenase Type 4: 0.1%； Hyaluronidase: 0.1%； Trypsin: 0.25% |
DMEM | Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes., J Assist Reprod Genet 31, 983-91, 2014 |
| 猪 | Porcine, female, <1 year | 脂肪间充质干细胞 / Adipose mesenchymal stem | Collagenase Type 1: 0.1% | DMEM | Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008 |
| 猪 | Porcine, male, 8 day | 生精上皮细胞 / Seminiferous epithelial cells | Collagenase: 0.15%； Deoxyribonuclease I: .0001%； Hyaluronidase: 0.15%； Trypsin: 0.05% |
DMEM/F12 | Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM, Biol Reprod 61, 225-30, 1999 |
| 兔 | Rabbit, New Zealand, 8-10 week | 肌腱细胞和肌腱干细胞 / Tenocytes and tendon stem cells | Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
DMEM | Zhang, J. and Wang, J.: Characterization of Differential Properties of Rabbit Tendon Stem Cells and Tenocytes., BMC Musculoskelet Disord 11, 10, 2010 |
| 大鼠 | Rat, SD, 2-3 month | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 1: 0.1% | DMEM | Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells, Cytotherapy 19, 937-946, 2017 |
| 大鼠 | Rat | 胎盘间充质 / Placental mesenchymal | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.02%； Neutral Protease: 0.1% |
DMEM | Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells., Sci Rep 7, 41837, 2017 |
| 大鼠 | Rat, 9 week | 脂肪基质细胞 / Adipose stromal | Collagenase: 0.1% | DMEM | Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury., Cytotherapy 19, 839-848, 2017 |
| 大鼠 | Rat | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.075% | DMEM | Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model., Cytotherapy 17, 543-59, 2015 |
| 大鼠 | Rat. SD, 5 month | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 2: 0.075% | DMEM | Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells., Cytotherapy 17, 215-23, 2015 |
| 大鼠 | Rat, 6-8 week | 脊髓神经祖细胞 / Spinal cord neural progenitor | PDS kit: with modifications | EBSS | Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015 |
| 大鼠 | Rat, adult | 肿瘤 / Tumor | Collagenase Type 1: 0.16%； Hyaluronidase: 0.002%； Deoxyribonuclease I: 0.006% |
DMEM/F12 | Duarte, S., Momier, D., Baque, P., Casanova, V., Loubat, A., Samson, M., Guigonis, J., Staccini, P., Saint-Paul, M., De Lima, M., Carle, G. and Pierrefite-Carle, V.: Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model., Stem Cells 31, 423-32, 2013 |
| 大鼠 | Rat, SD, neonatal | 棕色脂肪细胞 / Brown adipocytes | Collagenase Type 4: 0.1%； Neutral Protease: 0.1%； Trypsin: 0.05% |
PBS | Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose., J Biomed Biotechnol 2010, 104296, 2010 |
| 大鼠 | Rat | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.1% | DMEM | Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats., Stem Cells 27, 478, 2009 |
| 大鼠 | Rat, SD, newborn | 中性干细胞，雪旺细胞 / Neutral stem cells, Schwann cells | Trypsin: 0.25%； Collagenase: 0.16% |
DMEM/F12 | Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian: Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord, Dev Neurosci 27, 20-6, 2005 |
| 大鼠 | Rat, 2 month | 脊髓祖细胞，混合毒素 / Spinal cord progenitor cells，Hybrid toxins | PDS kit: see reference | Neurobasal A | Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells, J Histochem Cytochem 53(10), 1215, 2005 |
| 大鼠 | Rat, male, 200-250 g | 神经干细胞 / Neural stem cells | Papain: 0.09%； Deoxyribonuclease I: 0.1% |
EBSS | Gobbel GT, Choi SJ, Beier S, Niranjan A: Long-term cultivation of multipotential neural stem cells from adult rat subependyma, Brain Res 980, 221, 2003 |
| 大鼠 | Rat, postnatal, adult | 肠神经嵴干细胞 / Gut neural crest stem | Collagenase Type 4: 0.1%； Trypsin: 0.025% |
HBSS | Kruger, G., Mosher, J., Bixby, S., Joseph, N., Iwashita, T. and Morrison, S.: Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness., Neuron 35, 657, 2002 |
| 大鼠 | Rat, SD, embyronic | 坐骨神经和肠神经嵴干细胞 / Sciatic nerve and gut neural crest stem | Collagenase Type 4: 0.025%； Trypsin: 0.005%； Deoxyribonuclease I: 0.05% |
HBSS | Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity., Neuron 35, 643, 2002 |
| 大鼠 | Rat, adult | 神经干细胞 / Neural stem cells | Papain: 2.5 u/ml； Deoxyribonuclease I: 250 u/ml； Neutral Protease: 1 u/ml |
DMEM/F-12 | Palmer, T., Markakis, E., Willhoite, A., Safar, F., and Gage, F.: Fibroblast Growth Factor-2 Activates a Latent Neurogenic Program in Neural Stem Cells from Diverse Regions of the Adult CNS, J Neurosci 19, 8487, 1999 |
| 大鼠 | Rat | 中枢神经系统干细胞 / Central nervous system stem cells | Papain: 0.01%； Neutral Protease: 0.1% |
DMEM/F-12 | Ray, J. and Gage, F.: Neural Stem Cell Isolation, Characterization and Transplantation,Modern Techniques in Neuroscience Research,Johansson, H. and Windhorst, U., Springer-Verlag New York, 339, 1999 |
| 大鼠 | Rat, SD | 坐骨神经 / Sciatic nerves | Trypsin: 0.025%； Collagenase Type 3: 0.1% |
L-15 medium | Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999 |
| 大鼠 | Rat (also mice) | 脊髓 / Spinal cord | Trypsin: 0.133% | HBSS and PIPES | Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System, Cell 96, 25, 1999 |
| 大鼠 | Rat, embryonic | 神经元和祖细胞 / Neurons and progenitor | Papain: 20 u/ml | EBSS | Maric, D., Maric, I. and Barker, J.: Buoyant Density Gradient Fractionation and Flow Cytometric Analysis of Embryonic Rat Cortical Neurons and Progenitor Cells., Methods 16, 247, 1998 |
| 大鼠 | Rat | 干细胞，神经嵴 / Stem, neural crest | Collagenase: 0.075% | Ringer's solution | Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest, Cell 71, 973, 1992 |
