原代细胞分离中，针对生殖相关细胞组织涉及到黄体细胞，黄体、成纤维细胞、上皮，子宫内膜，卵巢，子宫和羊膜间充质干细胞等等的解离通常会使用酶，如何选择合适的酶来完成对应生殖相关细胞组织的解离，以及原代细胞采集的常规操作， 艾美捷针对生殖相关细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
| 生殖相关细胞 | |||||
| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 牛 | Bovine | 黄体细胞 / Corpus leuteal cells | Collagenase Type 4: 420 u/ml | M-199 | Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R: Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis, Endocrinology 142, 5254-60, 2001 |
| 牛 | Bovine, female | 黄体 / Leuteal | Collagenase Type 1: 0.2% | Ham's F-12 | Tsang PC, Poff JP, Boulton EP, Condon WA: Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro, Biol Reprod 53, 1160-8, 1995 |
| 牛 | Calf, mid to late gestational | 成纤维细胞 / Fibroblasts | Collagenase: 0.1% | Medium 199 | Coplen, D., Howard, P., Duckett, J., Snyder, H., and Macarak, E.: Characterization of a Fibroblast Cell From the Urinary Bladder Wall, In Vitro Cell Dev Biol 30A, 604, 1994 |
| 牛 | Bovine | 上皮，子宫内膜 / Epithelial，Endometrial | Collagenase Type 2: 0.1% | DMEM/EBSS | Munson, L., Chandler, S., and Schlafer, D.: Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3), 123, 1988 |
| 牛 | Bovine | 内，黄体，子宫内膜，卵巢，子宫 / Interna & corpus luteum，Endometrium，Ovarian，Uterine | Pronase: 0.1% | Moscona's BSS | Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984 |
| 犬/狗 | Canine | 羊膜间充质干细胞 / Amniotic membrane MSC | Trypsin: 0.25%； Collagenase Type 1: 0.2% |
LG-DMEM | Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012 |
| 鸡 | Chicken, Gallus Domesticus,20-30 week | 初级卵泡 / Primary follicles | Trypsin: 0.15%； Collagenase Type 1: 0.125% |
Dulbecco's phosphate buffered saline | Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong: An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles, J Reprod Dev 52, 569-76, 2006 |
| 鱼 | Sebastes Schlegel | 卵巢 / Ovarian | Collagenase Type 1: 500 u/ml | L-15 | Ryu, J., Kim, H., Bae, S., Jung, C. and Gong, S.: Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli, Fish Aquatic Sci 19, , 2016 |
| 青蛙 | Xenopus Laevis, female | 卵母细胞 / Oocytes | Collagenase Type 1: 0.2% | Barth's solution | Nicoll, G, Jawad, A., Weymouth, R, Zhang, H. and Beg, A.: Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans, Br J Pharmacol 174, 781, 2017 |
| 青蛙 | Xenopus laevis | 卵母细胞 / Oocytes | Collagenase: 0.2% | Barth's solution | Chatzidaki, A., D'Oyley, J., Gill-Thind, J., Sheppard, T. and Millar, N.: The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors., Neuropharmacol 97, 75-85, 2015 |
| 青蛙 | Xenopus | 卵母细胞 / Oocytes | Collagenase Type 2: 0.2% | see reference | O'Connell, D., Mruk, K., Rocheleau, J. and Kobertz, W.: Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings., J Gen Physiol 138, 271-7, 2011 |
| 青蛙 | Xenopus laevis, female | 卵母细胞 / Oocytes | Collagenase: 0.2% | see reference | Mruk, K. and Kobertz, W.: Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes., PLoS ONE 4, e4236, 2009 |
| 青蛙 | Xenopus laevis, female | 卵母细胞 / Oocytes | Collagenase: 0.5% | Barth's solution, CF | Cohen, S., Au, S. and Pante, N.: Microinjection of Xenopus Laevis Oocytes., J Vis Exp 24, 1106, 2009 |
| 青蛙 | Xenopus laevis, female | 卵母细胞 / Oocytes | Collagenase Type 1: 0.2% | see reference | Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio: Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species, J Biol Chem 277, 8912-9, 2002 |
| 青蛙 | Xenopus laevis, female | 卵母细胞 / Oocytes | Collagenase Type 1: 0.2% | CF Medium | Alagem, N., Dvir, M., and Reuveny, E.: Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues, J Physiol 534 (2), 381, 2001 |
| 青蛙 | Xenopus | 卵母细胞 / Oocytes | Collagenase Type 1: 1% | (see reference) | Tian, J., Kim, S., Heilig, E., and Ruderman, J.: Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation, Proc Natl Acad Sci U S A 97, 14358, 2000 |
| 青蛙 | Xenopus laevis, female | 卵母细胞 / Oocytes | Collagenase: 0.1% | Barth's solution, CF | Karkanias, N, and Papke, R.: Subtype-Specific Effects of Lithium on Glutamate Receptor Function, J Neurophysiol 81, 1506, 1999 |
| 青蛙 | Xenopus laevis, mature female | 卵母细胞 / Oocytes | Collagenase: 0.2% | CF Medium | Moriarty, T., Gillo, B., Carty, D., Premont, R., Landau, E., Iyengar, R.: Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C, Proc Natl Acad Sci U S A 85, 8865, 1988 |
| 仓鼠 | Hamster, Chinese | 子房 / Ovary | Trypsin: 5% | Dialyzed fetal calf serum, 10% and 0.5M Methotrexate | Wallis, R., and Drickamer, K.: Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins, J Biol Chem 274 (6), 3580, 1999 |
| 人 | Human | 子宫内膜上皮和间质成纤维细胞 / Endometrial epithelial and stromal fibroblasts | Collagenase Type 1: 0.32%； Hyaluronidase: 62 u/ml |
DMEM | Chen, J. and Roan, N.: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts., Bio Protoc 5, , 2015 |
| 人 | Human | 子宫内膜间质 / Endometrial stromal | Collagenase Type 3: 8 u/ml； Deoxyribonuclease I: 0.01% |
DMEM/F-12 | Evans, J. and Salamonsen, L.: Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade., Biol Reprod 90, 14, 2014 |
| 人 | Human | 内皮集落形成 / Endothelial colony forming | Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.1%； Neutral Protease: 0.075% |
HBSS | Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2, 839-47, 2013 |
| 人 | Human | 子宫上皮 / Uterine epithelial | Pancreatin: 0.34%； Hyaluronidase: 0.01%； Collagenase: 0.16% |
HBSS | Patel, M., Ghosh, M., Fahey, J. and Wira, C.: Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol., PLoS ONE 7, e35654, 2012 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.4%； Deoxyribonuclease I: 0.01% |
DMEM/F12 | Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1, 48, 2012 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 2: 10%； Neutral Protease: see reference |
CMF-DPBS | Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol Chapter 1, Unit 1E.2, 2007 |
| 人 | Human, female | 蜕膜 / Decidual | Collagenase: 0.25%； Deoxyribonuclease I: 6.25 u/ml |
DMEM/F12 | Lockwood, C., Arcuri, F., Toti, P., Felice, C., Krikun, G., Guller, S., Buchwalder, L. and Schatz, F.: Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis., Am J Pathol 169, 1294-302, 2006 |
| 人 | Human | 绒毛外滋养细胞和蜕膜基质细胞 / Extravillanous trophoblasts and decidual stromal | Trypsin: 0.1-0.25%； Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.02-0.05% |
HBSS RPMI | Spessotto, P., Bulla, R., Danussi, C., Radillo, O., Cervi, M., Monami, G., Bossi, F., Tedesco, F., Doliana, R. and Colombatti, A.: EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall., J Cell Sci 119, 4574, 2006 |
| 人 | Human | 支持细胞 / Sertoli cells | Trypsin: 2.5%； Collagenase Type 1: 2%； Hyaluronidase: 1% |
DMEM/F-12 | Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun: Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro, Chin Med J (Engl) 118, 1857-62, 2005 |
| 人 | Human, female | 子宫上皮 / Uterine epithelial | Pancreatin: 0.34%； Hyaluronidase: 0.01%； Collagenase: 0.16% |
HBSS | Meter, R., Wira, C. and Fahey, J.: Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture., Fertil Steril 84, 191, 2005 |
| 人 | Human, female, 34-51 yr | 子宫内膜上皮和基质细胞 / Endometrial epithelial and stromal cells | Collagenase Type 3: 0.03%； Deoxyribonuclease I: 0.004% |
DMEM/F-12 | Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells, Biol Reprod 70, 1738-50, 2004 |
| 人 | Human | 内皮胎盘 / Endothelial placental | Collagenase Type 1: 0.2%； Trypsin: 0.2%； Deoxyribonuclease I: 0.1% |
DMEM | Wang Xin, Athayde Neil, Trudinger Brian: Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease, Am J Obstet Gynecol 190, 596-601, 2004 |
| 人 | Human | 羊膜上皮和成纤维细胞 / Amnion epithelial and fibroblast | Trypsin: 0.125%； Collagenase: 0.1%； Deoxyribonuclease I: 0.02% |
PBS | Sun Kang, Myatt Leslie: Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts, Endocrinology 144, 5568-77, 2003 |
| 人 | Human | 子宫上皮细胞 / Uterine epithelial cells | Pancreatin: 0.34%； Collagenase: 0.16%； Hyaluronidase: 0.16% |
HBSS | Fahey John V, Wira Charles R: Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture, J Infect Dis 185, 1606-13, 2002 |
| 人 | Human, embryo | 内皮，造血，基质细胞 / Endothelial，Hematopoietic，Stromal | Collagenase Type 1/2/4: 0.1% | DMEM | Oberlin, E., Tavian, M., Blazsek, I., and Peault, B.: Blood-forming potential of vascular endothelium in the human embryo, Development 129, 4147, 2002 |
| 人 | Human, female | 间皮 / Mesothelial | Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.05% |
Eagle's MEM | Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S: Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells, Hum Reprod 17, 2832-8, 2002 |
| 人 | Human | 干细胞，胚 / Stem, embryonic | Neutral Protease: 0.01% - 0.02% | DMEM | Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells, Nat Biotechnol 19, 1129, 2001 |
| 人 | Human, 20-40 yr | 子宫内膜上皮细胞和基质细胞 / Endometrium epithelial and stromal cells | Collagenase Type 1: 0.2% | HBSS | Arnold, J., Kaufman, D., Seppala, M., and Lessey, B.: Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model, Hum Reprod 16, 836, 2001 |
| 人 | Human | 绒毛 / Chorionic villi | Trypsin: see reference； Collagenase Type 3: 100 u/ml |
HBSS | Yusuf RZ, Naeem R: Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination, Early Pregnancy 5, 121-31, 2001 |
| 人 | Human | 子宫内膜内皮细胞 / Endometrial endothelial cells | Collagenase Type 1: 0.2% | McCoys medium | Nikitenko LL, MacKenzie IZ,Rees MC, Bicknell R: Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium, Mol Hum Reprod 6, 811, 2000 |
| 人 | Human, female, 20-40 year | 微血管内皮细胞 / Microvascular endothelial cells | Collagenase Type 2: 0.2%； Deoxyribonuclease I: 0.0015%； Trypsin: 0.05% |
PBS | Gargett CE, Bucak K, Rogers PA: Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells, Hum Reprod 15, 293-301, 2000 |
| 人 | Human, female | 子宫内膜上皮细胞 / Endometrial epithelial cells | Collagenase Type 3: 45 u/ml； Deoxyribonuclease I: .00035% |
DMEM/F-12 | Zhang J, Lathbury LJ, Salamonsen LA: Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium, Biol Reprod 62, 404-11, 2000 |
| 人 | Human | 膜细胞 / Theca cells | Collagenase Type 1: 0.3%； Deoxyribonuclease I: 0.0005%； Hyaluronidase: 0.1% |
PBS | Runesson E, Ivarsson K, Janson P, Brannstrom M: Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle, J Clin Endocrinol Metab 85, 4387-95, 2000 |
| 人 | Human | 黄体细胞 / Corpus luteum cells | Collagenase Type 2: 0.25%； Deoxyribonuclease I: .005% |
PBS | Friden BE, Runesson E, Hahlin M, Brannstrom M: Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum, Mol Hum Reprod 6, 397-403, 2000 |
| 人 | Human, females, 25-45 yr | 黄体细胞 / Luteal cells | Collagenase Type 2: 0.25% | PBS | Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M: Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture, Mol Hum Reprod 5, 714-9, 1999 |
| 人 | Human, female, 25-41 year | 卵泡 / Follicles | Collagenase Type 2: 0.025-0.1% | EBSS | Hovatta O, Wright C, Krausz T, Hardy K, Winston RM: Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation, Hum Reprod 14, 2519-24, 1999 |
| 人 | Human | 胎盘 / Placental | Deoxyribonuclease I: 0.04%； Collagenase Type 2: 0.1% |
PSS | Bradbury., R., Sunn, K., Crossley, M., Bai, M., Brown, E., Delbridge, D., and Conigrave, A.: Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta, J Endocrinol 156, 425-430, 1998 |
| 人 | Human, female | 子宫内膜基质细胞 / Endometrial stromal cells | Collagenase: 4000 u/ml | DMEM/F-12 | Huang JC, Liu DY, Dawood MY: The expression of vascular endothelial growth factor isoforms in cultured human endometrial stromal cells and its regulation by 17beta-oestradiol, Mol Hum Reprod 4, 603-7, 1998 |
| 人 | Human | 间质子宫内膜 / Stromal endometrial | Collagenase Type 3: 45 u/ml； Deoxyribonuclease I: .00035% |
DMEM/Ham's F12 | Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA: Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation, Biol Reprod 59, 693-703, 1998 |
| 人 | Human | 上皮，输卵管 / Epithelial, fallopian tube | Collagenase Type 1: 1% | Medium 199 | Takeuchi, K., Maruyama, I., Yamamoto, S., Oki, T., Nagata, Y.: Isolation and Monolayer Culture of Human Fallopian Tube Epithelial Cells, In Vitro Cell Dev Biol 27, 720, 1991 |
| 人 | Human | 滋养细胞，胎盘 / Trophoblasts, placental | Trypsin: 0.25% | EBSS, CMF | Branchaud, C.L., Goodyer, C.G., Guyda, H.J. and Lefebvre, Y.: A Serum-Free System for Culturing Human Placental Trophoblasts, In Vitro Cell Dev Biol 26, 865, 1990 |
| 人 | Human | 滋养细胞，胎盘 / Trophoblasts, placental | Trypsin: 0.25% | PBS | Jie, Z., Fey, S., Hager, H., Hollsberg, P., Ebbesen, P., and Larsen, P.: Markers For Human Placental Trophoblasts in Two-Dimensional Gel Electrophoresis, In Vitro Cell Dev Biol 26, 937, 1990 |
| 人 | Human | 绒毛膜，胎盘 / Chorionic, placental | Deoxyribonuclease I: 0.003% | HBSS | Egan, D., Grzegorczyk, V., Tricarico, K., Rueter, A.H., Olleman, W., and Marcotte, P.: Human Placental Chorionic Renin: Production, Purification and Characterization, Biochim Biophys Acta 965, 68, 1988 |
| 人 | Human, female | 子宫内膜 / Endometrial | Collagenase: 2% | RPMI 1640 | Rinehart, C., Lyn-Cook, B., and Kaufman, D.: Gland formation from human endometrial epithelial cells in vitro, In Vitro Cell Dev Biol 24 (10), 1037, 1988 |
| 人 | Human | 胎盘 / Placental | Trypsin: 0.25% | DMEM | Morrish, D., and Siy, O.: Critical Factors in Establishing Monolayer Cultures of Normal Human Placental Cells in Serum-Free Medium, Endocr Res 12 (3), 229, 1986 |
| 人 | Human | 内，黄体，子宫内膜，卵巢，子宫 / Interna & corpus luteum，Endometrium，Ovarian，Uterine | Pronase: 0.1% | Moscona's BSS | Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984 |
| 人 | Human, female, 27-49 years | 上皮，卵巢 / Epithelial，Ovary | Trypsin: 0.125% | HBSS, CMF | Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture, In Vitro 20, 743, 1984 |
| 人 | Human | 上皮，基质细胞 / Epithelial，Stromal | Collagenase: 0.25% | See reference | Siegfried, J., Nelson, K., Martin, J., and Kaufman, D.: Histochemical Identification of Cultured Cells From Human Endometrium, In Vitro 20 (1), 25, 1984 |
| 人 | Human, infant and neonate | 上皮，前列腺 / Epithelial，Prostate | Trypsin: 0.1% | HBSS | Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures, Methods Cell Biol 21, 195, 1980 |
| 人 | Human, female, 27 years | 平滑肌，子宫 / Smooth muscle, uterine | Trypsin: 0.05% | EBSS | Rifas, L., Fant, J., Makman, M., and Seifter, S.: The Characterization of Human Uterine Smooth Muscle Cells in Culture, Cell Tissue Res 196, 385, 1979 |
| 人 | Human | 上皮，基质细胞 / Epithelial，Stromal | Collagenase Type 1: 180 u/ml | DMEM | Kirk, D., King, R., Heyes, J., Peachey, L., Hirsch, P., and Taylor, W.: Normal Human Edometrium in Cell Culture, In Vitro 14 (8), 651, 1978 |
| 昆虫 | Drosophila | 卵巢 / Ovarian | Collagenase Type 2: 0.1% | Grace's | Ma, X., Wang, S., Do, T., Song, X., Inaba, M., Nishimoto, Y., Liu, L., Gao, Y., Mao, Y., Li, H., McDowell, W., Park, J., Malanowski, K., Peak, A., Perera, A., Li, H. et al.: Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary., PLoS ONE 9, e90267, 2014 |
| 昆虫 | Drosophila, embryonic | 心鉴别 / Cardiac differentiating | Collagenase Type 1: 20 u/ml； Trypsin: 0.25%； Deoxyribonuclease I: 4 u/ml |
Schneider | Salmand, P. Iche-Torres, M. and Perrin, L.: Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis.,Fly (Austin) Vol. 5,, , 261, |
| 猴 | Rhesus macaque | 卵泡 / Follicle | Collagenase Type 1: 275 u/m； Deoxyribonuclease I: 585 u/ml |
HEPES | Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB and Stouffer, RL: Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin., Hum Reprod 26, 1061-72, 2011 |
| 小鼠 | Mouse, 4 week | 滋养层 / Trophoblast | Collagenase Type 1: 0.1%； Collagenase Type 2: 0.25% |
DMEM | Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells, Stem Cell Res Ther 11, 55, 2020 |
| 小鼠 | Mouse, adult | 睾丸 / Testes | Collagenase Type 1: 120 u/ml； Deoxyribonuclease I: 0.1%； Trypsin: 5% |
MEM | Lee, A, Rusch, J., Lima, A., Usmani, A., Huang, N., Lepamets, M., Vigh-Conrad, K., Worthington, R., et al: Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility., Nat Commun 10, 4626, 2019 |
| 小鼠 | Mouse, 1-7 day | 睾丸 / Testicular | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.1% |
HBSS | Kang, H., Chen, L., Lichti-Kaiser, K., Liao, G., Gerrish, K., Bortner, C., Yao, Hu., Eddy, E. and Jetten, A.: Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis., Stem Cells 34, 2772-2783, 2016 |
| 小鼠 | Mouse, adult | 精原干细胞 / Spermatogonial stem | Trypsin: 0.05%； Collagenase Type 1: 0.03%； Deoxyribonuclease I: 80 u/ml |
DMEM | Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines., J Vis Exp 72, e50017, 2013 |
| 小鼠 | Mouse | 卵巢 / Ovarian | Collagenase Type 4: 800 u/ml | HBSS | White, Y., Woods, D., Takai, Y., Ishihara, O., Seki, H. and Tilly, J.: Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen., Nat Med 18, 413, 2012 |
| 小鼠 | Mouse, female | 淋巴细胞 / Lymphocytes | Collagenase: 450 u/ml | RPMI | Jiang, J. and Kelly, K.: Isolation of Lymphocytes from Mouse Genital Tract Mucosa., J Vis Exp 67, e4391, 2012 |
| 小鼠 | Mouse | 阴道平滑肌 / Vaginal smooth muscle | Collagenase Type 2: 175 u/ml； Elastase: 0.025% |
DMEM/F12 | Venkataraman, L., Lenis, A., Couri, B., Damaser, M. and Ramarurthi, A: Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse, J Tissue Sci Eng 3, 3, 2012 |
| 小鼠 | Mouse, male | 睾丸，减数分裂 / Testis, meiotic | Collagenase Type 1: 120 u/ml； Deoxyribonuclease I: 0.001%； Trypsin: 0.1% |
Gey's BSS | Getun, I., Torres, B. and Bois, P.: Flow Cytometry Purification of Mouse Meiotic Cells., J Vis Exp 50, 2602, 2011 |
| 小鼠 | Mouse | 透明带, 无卵细胞 / Zona pellucid free eggs | Collagenase: 0.1% | THY-HEPES | Yamatoya, K., Ito, C., Araki, M., Furuse, R. and Toshimori, K.: One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation., Reprod Med Bio 10, 97-103, 2011 |
| 小鼠 | Mouse | 子宫造口 / Uterine stomal | Neutral Protease: 0.6%； Pancreatin: 0.25%； Collagenase Type 3: 0.05% |
DMEM/F12 | Chen, L., Belton, R. and Nowak, R.: Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation., Endocrinology 150, 966, 2009 |
| 小鼠 | Mouse | 生殖细胞 / Germ cells | Collagenase Type 1: 100 u/ml | HBSS | Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells., Proc Natl Acad Sci U S A 105, 10420, 2008 |
| 小鼠 | Mouse, embryonic | 造血干细胞 / Hematopoietic stem cells | Collagenase: 0.1% | PBS | Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta., J Vis Exp 16, e742, 2008 |
| 小鼠 | Mouse, 6 day | 塞尔托利 / Sertoli | Collagenase Type 2: 500 u/ml； Hyaluronidase: 0.1%； Deoxyribonuclease I: 0.0005% |
DMEM | Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta: Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse, Biol Reprod 69, 985-94, 2003 |
| 小鼠 | Mouse, adult, neonatal and fetal | 睾丸细胞 / Testicular cells | Collagenase Type 1: 0.1% | DMEM/F-12 | O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ: Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis, Endocrinology 144, 3279-84, 2003 |
| 小鼠 | Mouse, male, 3.5 months old | 睾丸间质 / Leydig | Collagenase: 0.06% | Medium E 199 | Faldikova, L., Diblikova, I., Canderle, J., Zraly, Z., Veznik, Z., and Sulcova, A.: Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production, Vet Med 46 (6), 160, 2001 |
| 小鼠 | Mouse, male, 12-14-week-old | 曲细精管 / Seminiferous tubules | Trypsin: 0.05% | DMEM | Lin, Q., Sirotkin, A., and Skoultchi, A.: Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t, Mol Cell Biol 20 (6), 2122, 2000 |
| 小鼠 | Mouse, 6-12 day | 卵母细胞-颗粒 / Oocyte-granulosa | Collagenase Type 1: 0.1%； Deoxyribonuclease I: 0.02% |
Waymouth | Eppig JJ, O'Brien MJ: Development in vitro of mouse oocytes from primordial follicles, Biol Reprod 54, 197-207, 1996 |
| 小鼠 | Mouse, female,6-8 weeks | 子宫 / Uterine | Trypsin: 0.2% | HBSS | Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes, In Vitro Cell Dev Biol 27A, 713, 1991 |
| 小鼠 | Mouse, female, 21 day, 10-12 g | 阴道上皮 / vaginal epithelial | Collagenase Type 3: 38 u/ml | DMEM | Tsai, P., Uchima, F., Hamamoto, S., and Bern, H.: Proliferation and Differentiation of Prepubertal Mouse Vaginal Epithelial Cells In Vitro and the Specificity of Estrogen-Induced Growth Retardation, In Vitro Cell Dev Biol 27A, 461, 1991 |
| 小鼠 | Mouse, CF1, female | 积云，单细胞胚胎 / Cumulus, one-cell embryos | Hyaluronidase: 0.1% | PBS, CMF | Spindle, A.: In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition, In Vitro Cell Dev Biol 26, 151, 1990 |
| 小鼠 | Mouse (BALB/cCRGL), male, 2-3 months | 上皮，前列腺 / Epithelial, prostate gland | Hyaluronidase: 0.1% | Medium 199 | Turner, T., Bern, H., Young,P., and Cunha, G.: Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel, In Vitro Cell Dev Biol 26, 722, 1990 |
| 小鼠 | Mouse (C57B/T), fetus, 16-17 days old | 前列腺 / Prostate | Trypsin: 1.0% | HBSS/ DMEM | Thompson, T.C., Southgate, J., Kitchener, G., and Land, H.: Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ, Cell 56, 917, 1989 |
| 小鼠 | Mouse, outbred, CD-1, 21-23 days old | 上皮 / Epithelial | Trypsin: 0.5% | Medium 199 | Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro, Endocrinology 118 (3), 1011, 1986 |
| 小鼠 | Mouse, BALB/c | 上皮，间质 / Epithelial，Mesencymal | Trypsin: 1% | DMEM | Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells, Am J Physiol 251, E630, 1986 |
| 小鼠 | Mouse, BALB/cCrg, 40 days old | 上皮 / Epithelial | Trypsin: 1% | Medium 199 | Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma, Proc Natl Acad Sci U S A 83, 2109, 1986 |
| 小鼠 | Mouse, male, 10-13 wk | 睾丸，睾丸 / Leydig，Testis | Deoxyribonuclease I: 0.001% | Medium 199 w/ BSA | Stalvcey, J. and Payne, A.: Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains, Endocrinology 112, 1696, 1983 |
| 小鼠 | Mouse (BALB/cCrgl), female, 50 - 60 days | 上皮，阴道 / Epithelial, vagina | Collagenase Type 3: 0.1% | HBSS | Iguchi, T., Uchima, F.A., Ostrander, P., and Bern, H.: Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels, Proc Natl Acad Sci U S A 80, 3743, 1983 |
| 绵羊 | Ovine, 3-5 year | 子宫内膜间质 / Endometrial stromal | Collagenase Type 1: 0.5%； Deoxyribonuclease I: 0.04 u/ml |
DMEM/F-12 | Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium., PLoS ONE 10, e0127531, 2015 |
| 绵羊 | Sheep | 上皮 / Epithelial | Collagenase: 125 - 190 u/ml | DMEM | Salamonsen, L., Sum O, W., Doughton, B., and Findlay, J.: The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium, Endocrinology 117 (5), 2148, 1985 |
| 猪 | Porcine, 1-4 day | 睾丸 / Testicular | Collagenase Type 4: 0.1%； Hyaluronidase: 0.1%； Trypsin: 0.25% |
DMEM | Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes., J Assist Reprod Genet 31, 983-91, 2014 |
| 猪 | Porcine, male, 8 day | 生精上皮细胞 / Seminiferous epithelial cells | Collagenase: 0.15%；
Deoxyribonuclease I: .0001%； Hyaluronidase: 0.15%； Trypsin: 0.05%； |
DMEM/F12 | Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM, Biol Reprod 61, 225-30, 1999 |
| 猪 | Porcine, female | 黄体 / Corpus Leuteum | Collagenase Type 4: 600 u/ml | Medium 199 | Ciereszko, R., Petroff, B., Ottobre, A., Guan, Z., Stokes, B., and Ottobre, J.: Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs, J Endocrinol 159, 201, 1998 |
| 猪 | Porcine (also bovine, human) | 内黄体， 子宫内膜， 卵巢， 子宫， / Interna & corpus luteum， Endometrium， Ovarian， Uterine， |
Pronase: 0.1% | Moscona's BSS | Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984 |
| 猪 | Porcine, 3-4 week | 睾丸，睾丸 / Leydig，Testis | Trypsin: 0.0003% | Lebovitz L-15 Medium | Mather, J., Saez, J., and Haour, F.: Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells, Endocrinology 110, 933, 1982 |
| 兔 | Rabbit, New Zealand, 3 month | 睾丸细菌 / Testicular germ | Collagenase Type 1: 0.1%； Trypsin: 0.25%； Deoxyribonuclease I: 0.7% |
HBSS | Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties., FASEB J 25, 2604, 2011 |
| 兔 | Rabbit, New Zealand, 3 month | 睾丸细菌 / Testicular germ | Collagenase Type 1: 0.1%； Trypsin: 0.25%； Deoxyribonuclease I: 0.7% |
HBSS | Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties., FASEB J 25, 2604, 2011 |
| 兔 | Rabbit, New Zealand white, 4-6 month old, | 卵巢，间皮 / Ovarian，Mesothelial | Collagenase Type 1: 300 u/ml | HBSS | Setrakian, S., Oliveros-Saunders, B., and Nicosia, S.: Growth Stimulation of Ovarian and Extraovarian Mesothelial Cells by Corpus Luteum Extract, In Vitro Cell Dev Biol 29A, 879, 1993 |
| 兔 | Rabbit, New Zealand white, adult, female (nonpregnant), | 肌细胞，子宫 / Myocytes, uterine | Deoxyribonuclease I: 200 µg/ml | HBSS-HEPES buffer | Phillippe, M., Saunders, T., and Bangalore, S.: Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes, In Vitro Cell Dev Biol 26, 369, 1990 |
| 兔 | Rabbit, New Zealand, mature, female, 4-5 Kg | 卵巢表面上皮和腹膜间皮 / Ovarian surface epithelial and peritoneal mesothelial | Collagenase Type 1: 300 IU/ml (280 IU/mg) | HBSS, CMF | Piquette, G., and Timms, B.: Isolation and Characterization of Rabbit Ovarian Surface Epithelium, Granulosa Cells, and Peritoneal Mesothelium in Primary Culture, In Vitro Cell Dev Biol 26, 471, 1990 |
| 兔 | Rabbit, mixed breed | 子宫内膜上皮 / Endometrial epithelial | Collagenase Type 1: 0.005% | DMEM | Mulholland, J., Winterhager, E., and Beier, H.: Changes in Proteins Synthesized by Rabbit Endometrial Epithelial Cells Following Primary Culture, Cell Tissue Res 252, 123, 1988 |
| 兔 | Rabbit, New Zealand white, mature, 3-4 Kg | 子宫肌层 / Myometrial | Trypsin: 0.02%, 0.03%, 0.0375% | HBSS | Boulet, A., and Fortier, M.: Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment, In Vitro Cell Dev Biol 23, 93, 1987 |
| 兔 | Rabbit, New Zealand white estrous, female, 4-5 months | 间皮和表面上皮，卵巢 / Mesothelial and surface epithelial，Ovaries | Trypsin: 0.125%-0.5% | Medium 199 | Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium), Int J Gynecol Pathol 3, 348, 1984 |
| 大鼠 | Rat | 胎盘间充质 / Placental mesenchymal | Collagenase Type 4: 0.1%； Deoxyribonuclease I: 0.02%； Neutral Protease: 0.1% |
DMEM | Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells., Sci Rep 7, 41837, 2017 |
| 大鼠 | Rat, SD, 350-450 g | 睾丸间质 / Leydig cells | Collagenase: .05-0.1% | Medium 199 | Sharma RS, Pal PC, Rajalakshmi M.: Isolation and Culture of Leydig Cells from Adult Rats, Indian J Clinical Biochem 21, 27, 2006 |
| 大鼠 | Rat, SD, adult, male, 8 - 10 week | 曲细精管 / Seminiferous tubules | Trypsin: 0.05% | Krebs-Ringer bicarbonate buffer | Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization, Biol Proced Online 3 (1), 35, 2001 |
| 大鼠 | Rat, SD, immature | 子房 / Ovary | Collagenase Type 1: 144 u/ml | McCoy's 5a | Ando, M., Kol, S., Irahara, M., Sirois, J., and Adashi, E.: Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process, Mol Cell Endocrinol 157, 21, 1999 |
| 大鼠 | Rat, Han-Wistar, adult, male, 250 g | 睾丸 / Testes | Trypsin: 0.1% | Medium 199 w/ Hank's salts | Leckie, C., Welberg, L., and Seckl, J.: 11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells, J Endocrinol 159, 233, 1998 |
| 大鼠 | Rat, SD, female, immature, 26 day | 黄体 / Corpus luteum | Neutral Protease: 2.4 u/ml Deoxyribonuclease: 200u/ml | Serum-free medium | Tellieria, C., Ou, J., Sugino, N., Ferguson, S., and Gibori, G.: The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid, Endocrinology 139 (8), 3597, 1998 |
| 大鼠 | Rat, SD, 25 day | 卵巢膜间质 / Ovarian theca-interstitial | Collagenase Type 1: 0.5%； Deoxyribonuclease I: 0.02% |
Medium 199 | Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR: Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells, Biol Reprod 56, 891-7, 1997 |
| 大鼠 | Rat, SD, male, 90 days | 塞尔托利 / Sertoli | Hyaluronidase: 0.1% | DMEM | Onoda, M. and Djakiew, D.: Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin, In Vitro Cell Dev Biol 27, 215, 1991 |
| 大鼠 | Rat, SD, male | 睾丸间质 / Leydig | Trypsin: 0.02% | DMEM | Abayasekara, D., Kurlak, L., Band, A., Sullivan, M., and Cooke, B.: Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis, In Vitro Cell Dev Biol 27, 253, 1991 |
| 大鼠 | Rat, neonatal | 子宫 / Uterine | Trypsin: 1% | HBSS | Branham, W., Lyn-Cook, B., Andrews, A., McDaniel, M., Sheehan, D.: Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix, In Vitro Cell Dev Biol 27, 442, 1991 |
| 大鼠 | Rat, SD, male, 120-160 g | Leydig，肾上腺 / Leydig，Adrenal | Collagenase Type 2: 0.03% (adrenal) | Krebs Ringer bicarbonate buffer | Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26, 24, 1990 |
| 大鼠 | Rat, Wistar, adult | 子宫 / Uterine | Trypsin: 0.5% | PBS | Pampfer, S., Vanderheyden, I., Michiels, B., and DeHertogh, R.: Co-Culture of Two-Cell Rat Embryos on Cell Monolayers, In Vitro Cell Dev Biol 26, 944, 1990 |
| 大鼠 | Rat, SD, 10 day | 支持细胞 / Sertoli cells | Collagenase Type 2: 500 u/ml； Deoxyribonuclease I: 0.0005%； Hyaluronidase: 0.1% |
PBS | Hadley MA, Weeks BS, Kleinman HK, Dym M: Laminin promotes formation of cord-like structures by Sertoli cells in vitro, Dev Biol 140, 318-27, 1990 |
| 大鼠 | Rat, immature | 黄体，卵巢 / Luteal, ovaries | Collagenase: 0.3% | McCoy's | Rajan, V. and Menon, K.: Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary, Biochim Biophys Acta 959, 206, 1988 |
| 大鼠 | Rat, male, 20 day | 塞尔托利 / Sertoli | Trypsin: 0.15% | (see reference) | Skinner, M., Fetterolf, P., and Anthony, C.: Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function, J Biol Chem 263, 2884, 1988 |
| 大鼠 | Rat, SD, female | 管腔上皮 / Luminal epithelial | Trypsin: 0.5% | HBSS | Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells, J Cell Biol 107 (6), 2409, 1988 |
| 大鼠 | Rat, Wistar, virgin, female, 250 g | 阴道上皮 / Vaginal epithelial | Trypsin: 0.5% | PBS | Conti, C., and Tasat, D.: Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone, J Steroid Biochem 24 (3), 747, 1986 |
| 大鼠 | Rat, SD, female, 21 day | 黄体，卵巢 / Luteal, ovaries | Deoxyribonuclease I: 0.0004% | McCoy's | Rajan, V. and Menon, K.: Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells, Endocrinology 117, 2408, 1985 |
| 大鼠 | Rat, SD, male, 10 day | 塞尔托利 / Sertoli | Trypsin: 0.025% | DMEM | Hadley, M., Byers, S., Suarez-Quian, C., Kleinman, H., and Dym, M.: Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro, J Cell Biol 101, 1511, 1985 |
| 大鼠 | Rat, Holtzman, female, immature | 子宫 / Uterine | Deoxyribonuclease I: 0.025% | DMEM buffered with HEPES | Kassis, J., Walent, J., and Gorski, J.: Estrogen Receptors in Rat Uterine Cell Cultures: Effects of Medium on Receptor Concentration, Endocrinology 115, 762, 1984 |
| 大鼠 | Rat, SD, adult,male, 50-70 days | 睾丸 / Testicular | Deoxyribonuclease I: 10 µg/ml | HEPES | Hsueh, A., Bambino, T., Zhuang, L., Welsh, T., and Ling, N.: Mechanism of the Direct Action of Gonadotropin Releasing Hormone and Its Antagonist on Androgen Biosynthesis by Cultured Rat Testicular Cells, Endocrinology 112, 1653, 1983 |
| 大鼠 | Rat, SD, female, pseudopregnant, 21 day | 黄体，卵巢 / Luteal, ovaries | Hyaluronidase: 0.1% | EBSS | Rajendran, K., Hwang, J., and Menon, K.: Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells, Endocrinology 112, 1746, 1983 |
| 大鼠 | Rat, SD, 4-6 day, male | Sertolli，曲细小管 / Sertolli, seminiforous tubules | Collagenase: 0.03% | Serum-free medium | Rich, K., Bardin, C., Gunsalus, G., and Mather, J.: Age-Dependent Pattern of Androgen-Binding Protein Secretion from Rat Sertoli Cells in Primary Culture, Endocrinology 113, 2284, 1983 |
| 大鼠 | Rat, SD, female, 26 days | 黄体 / Luteal | Deoxyribonuclease I: 0.0004% | Medium 199 | Azhar, S. and Reaven, E.: Effect of Antimicrotubule Agents on Microtubules and Steroidogenesis in Luteal Cells, Am J Physiol 243, E380, 1982 |
| 大鼠 | Rat, SD, male | 睾丸间质 / Leydig | Collagenase: 0.1% | Krebs Ringer bicarbonate buffer | Ramachandran, J., and Sairam, M.R.: The Effects Of Interstitial Cell-Stimulating Hormone, Its Subunits,and Recombinants on Isolated Rat Leydig Cells, Arch Biochem Biophys 167, 294, 1975 |
