worthington 生殖相关细胞组织分离指南

原代细胞分离中,针对生殖相关细胞组织涉及到黄体细胞,黄体、成纤维细胞、上皮,子宫内膜,卵巢,子宫和羊膜间充质干细胞等等的解离通常会使用酶,如何选择合适的酶来完成对应生殖相关细胞组织的解离,以及原代细胞采集的常规操作, 艾美捷针对生殖相关细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:

worthington 胰腺组织分离指南

 

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

 

生殖相关细胞
种属 种属详情 细胞名称 酶组合推荐,含推荐浓度 培养基 参考文献
Bovine 黄体细胞 / Corpus leuteal cells Collagenase Type 4: 420 u/ml M-199 Levy N, Gordin M, Mamluk R, Yanagisawa M, Smith M F, Hampton J H, Meidan R: Distinct cellular localization and regulation of endothelin-1 and endothelin-converting enzyme-1 expression in the bovine corpus luteum: implications for luteolysis, Endocrinology 142, 5254-60, 2001
Bovine, female 黄体 / Leuteal Collagenase Type 1: 0.2% Ham's F-12 Tsang PC, Poff JP, Boulton EP, Condon WA: Four-day-old bovine corpus luteum: progesterone production and identification of matrix metalloproteinase activity in vitro, Biol Reprod 53, 1160-8, 1995
Calf, mid to late gestational 成纤维细胞 / Fibroblasts Collagenase: 0.1% Medium 199 Coplen, D., Howard, P., Duckett, J., Snyder, H., and Macarak, E.: Characterization of a Fibroblast Cell From the Urinary Bladder Wall, In Vitro Cell Dev Biol 30A, 604, 1994
Bovine 上皮,子宫内膜 / Epithelial,Endometrial Collagenase Type 2: 0.1% DMEM/EBSS Munson, L., Chandler, S., and Schlafer, D.: Long-Term Culture of Bovine Trophoblastic Cells, J Tiss Cul Meth 11 (3), 123, 1988
Bovine 内,黄体,子宫内膜,卵巢,子宫 / Interna & corpus luteum,Endometrium,Ovarian,Uterine Pronase: 0.1% Moscona's BSS Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984
犬/狗 Canine 羊膜间充质干细胞 / Amniotic membrane MSC Trypsin: 0.25%;
Collagenase Type 1: 0.2%
LG-DMEM Park, S., Seo, M., Kim, H. and Kang, K.: Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells., PLoS ONE 7, e44693, 2012
Chicken, Gallus Domesticus,20-30 week 初级卵泡 / Primary follicles Trypsin: 0.15%;
Collagenase Type 1: 0.125%
Dulbecco's phosphate buffered saline Du Meihong, Han Haitang, Jiang Bin, Zhao Chen, Qian Changsong, Shen Haiyan, Xu Yan, Li Zandong: An efficient isolation method for domestic hen (Gallus domesticus) ovarian primary follicles, J Reprod Dev 52, 569-76, 2006
Sebastes Schlegel 卵巢 / Ovarian Collagenase Type 1: 500 u/ml L-15 Ryu, J., Kim, H., Bae, S., Jung, C. and Gong, S.: Isolation and in Vitro Culture of Primary Cell Populations Derived from Ovarian Tissues of the Rockfish, Sebastes schlegeli, Fish Aquatic Sci 19, , 2016
青蛙 Xenopus Laevis, female 卵母细胞 / Oocytes Collagenase Type 1: 0.2% Barth's solution Nicoll, G, Jawad, A., Weymouth, R, Zhang, H. and Beg, A.: Pharmacological characterization of the excitatory ‘Cys-loop’ GABA receptor family in Caenorhabditis elegans, Br J Pharmacol 174, 781, 2017
青蛙 Xenopus laevis 卵母细胞 / Oocytes Collagenase: 0.2% Barth's solution Chatzidaki, A., D'Oyley, J., Gill-Thind, J., Sheppard, T. and Millar, N.: The Influence of Allosteric Modulators and Transmembrane Mutations on Desensitisation and Activation of alpha7 Nicotinic Acetylcholine Receptors., Neuropharmacol 97, 75-85, 2015
青蛙 Xenopus 卵母细胞 / Oocytes Collagenase Type 2: 0.2% see reference O'Connell, D., Mruk, K., Rocheleau, J. and Kobertz, W.: Xenopus laevis Oocytes Infected with Multi-drug-resistant Bacteria: Implications for Electrical Recordings., J Gen Physiol 138, 271-7, 2011
青蛙 Xenopus laevis, female 卵母细胞 / Oocytes Collagenase: 0.2% see reference Mruk, K. and Kobertz, W.: Discovery of a Novel Activator of KCNQ1-KCNE1 K Channel Complexes., PLoS ONE 4, e4236, 2009
青蛙 Xenopus laevis, female 卵母细胞 / Oocytes Collagenase: 0.5% Barth's solution, CF Cohen, S., Au, S. and Pante, N.: Microinjection of Xenopus Laevis Oocytes., J Vis Exp 24, 1106, 2009
青蛙 Xenopus laevis, female 卵母细胞 / Oocytes Collagenase Type 1: 0.2% see reference Pannaccione Anna, Castaldo Pasqualina, Ficker Eckhard, Annunziato Lucio, Taglialatela Maurizio: Histidines 578 and 587 in the S5-S6 linker of the human Ether-a-gogo Related Gene-1 K+ channels confer sensitivity to reactive oxygen species, J Biol Chem 277, 8912-9, 2002
青蛙 Xenopus laevis, female 卵母细胞 / Oocytes Collagenase Type 1: 0.2% CF Medium Alagem, N., Dvir, M., and Reuveny, E.: Mechanism of Ba2+ Block of a Mouse Inwardly Rectifying K+ Channel: Differential Contribution by Two Discrete Residues, J Physiol 534 (2), 381, 2001
青蛙 Xenopus 卵母细胞 / Oocytes Collagenase Type 1: 1% (see reference) Tian, J., Kim, S., Heilig, E., and Ruderman, J.: Identification of XPR-1, A Progesterone Receptor Required for Xenopus Oocyte Activation, Proc Natl Acad Sci U S A 97, 14358, 2000
青蛙 Xenopus laevis, female 卵母细胞 / Oocytes Collagenase: 0.1% Barth's solution, CF Karkanias, N, and Papke, R.: Subtype-Specific Effects of Lithium on Glutamate Receptor Function, J Neurophysiol 81, 1506, 1999
青蛙 Xenopus laevis, mature female 卵母细胞 / Oocytes Collagenase: 0.2% CF Medium Moriarty, T., Gillo, B., Carty, D., Premont, R., Landau, E., Iyengar, R.: Beta gamma Subunits of GTP-Binding Proteins Inhibit Muscarinic Receptor Stimulation of Phospholipase C, Proc Natl Acad Sci U S A 85, 8865, 1988
仓鼠 Hamster, Chinese 子房 / Ovary Trypsin: 5% Dialyzed fetal calf serum, 10% and 0.5M Methotrexate Wallis, R., and Drickamer, K.: Molecular Determinants of Oligomer Formation and Complement Fixation in Mannose-Binding Proteins, J Biol Chem 274 (6), 3580, 1999
Human 子宫内膜上皮和间质成纤维细胞 / Endometrial epithelial and stromal fibroblasts Collagenase Type 1: 0.32%;
Hyaluronidase: 62 u/ml
DMEM Chen, J. and Roan, N.: Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts., Bio Protoc 5, , 2015
Human 子宫内膜间质 / Endometrial stromal Collagenase Type 3: 8 u/ml;
Deoxyribonuclease I: 0.01%
DMEM/F-12 Evans, J. and Salamonsen, L.: Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade., Biol Reprod 90, 14, 2014
Human 内皮集落形成 / Endothelial colony forming Collagenase Type 1: 0.1%;
Deoxyribonuclease I: 0.1%;
Neutral Protease: 0.075%
HBSS Patel, J., Seppanen, E., Chong, M., Yeo, J., Teo, E., Chan, J., Fisk, N. and Khosrotehrani, K.: Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta., Stem Cells Transl Med 2, 839-47, 2013
Human 子宫上皮 / Uterine epithelial Pancreatin: 0.34%;
Hyaluronidase: 0.01%;
Collagenase: 0.16%
HBSS Patel, M., Ghosh, M., Fahey, J. and Wira, C.: Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol., PLoS ONE 7, e35654, 2012
Human 间充质干细胞 / Mesenchymal stem Collagenase Type 1: 0.4%;
Deoxyribonuclease I: 0.01%
DMEM/F12 Shalini, V., Pratheep, S., Muhammad, A., Sharmili, V., Elizabeth, G. and Rajesh, R.: Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta, Regenerative Research 1, 48, 2012
Human 间充质干细胞 / Mesenchymal stem Collagenase Type 2: 10%;
Neutral Protease: see reference
CMF-DPBS Steigman, S. and Fauza, D.: Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta., Curr Protoc Stem Cell Biol Chapter 1, Unit 1E.2, 2007
Human, female 蜕膜 / Decidual Collagenase: 0.25%;
Deoxyribonuclease I: 6.25 u/ml
DMEM/F12 Lockwood, C., Arcuri, F., Toti, P., Felice, C., Krikun, G., Guller, S., Buchwalder, L. and Schatz, F.: Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis., Am J Pathol 169, 1294-302, 2006
Human 绒毛外滋养细胞和蜕膜基质细胞 / Extravillanous trophoblasts and decidual stromal Trypsin: 0.1-0.25%;
Collagenase Type 1: 0.1%;
Deoxyribonuclease I: 0.02-0.05%
HBSS RPMI Spessotto, P., Bulla, R., Danussi, C., Radillo, O., Cervi, M., Monami, G., Bossi, F., Tedesco, F., Doliana, R. and Colombatti, A.: EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall., J Cell Sci 119, 4574, 2006
Human 支持细胞 / Sertoli cells Trypsin: 2.5%;
Collagenase Type 1: 2%;
Hyaluronidase: 1%
DMEM/F-12 Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun: Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro, Chin Med J (Engl) 118, 1857-62, 2005
Human, female 子宫上皮 / Uterine epithelial Pancreatin: 0.34%;
Hyaluronidase: 0.01%;
Collagenase: 0.16%
HBSS Meter, R., Wira, C. and Fahey, J.: Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture., Fertil Steril 84, 191, 2005
Human, female, 34-51 yr 子宫内膜上皮和基质细胞 / Endometrial epithelial and stromal cells Collagenase Type 3: 0.03%;
Deoxyribonuclease I: 0.004%
DMEM/F-12 Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E: Clonogenicity of human endometrial epithelial and stromal cells, Biol Reprod 70, 1738-50, 2004
Human 内皮胎盘 / Endothelial placental Collagenase Type 1: 0.2%;
Trypsin: 0.2%;
Deoxyribonuclease I: 0.1%
DMEM Wang Xin, Athayde Neil, Trudinger Brian: Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease, Am J Obstet Gynecol 190, 596-601, 2004
Human 羊膜上皮和成纤维细胞 / Amnion epithelial and fibroblast Trypsin: 0.125%;
Collagenase: 0.1%;
Deoxyribonuclease I: 0.02%
PBS Sun Kang, Myatt Leslie: Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts, Endocrinology 144, 5568-77, 2003
Human 子宫上皮细胞 / Uterine epithelial cells Pancreatin: 0.34%;
Collagenase: 0.16%;
Hyaluronidase: 0.16%
HBSS Fahey John V, Wira Charles R: Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture, J Infect Dis 185, 1606-13, 2002
Human, embryo 内皮,造血,基质细胞 / Endothelial,Hematopoietic,Stromal Collagenase Type 1/2/4: 0.1% DMEM Oberlin, E., Tavian, M., Blazsek, I., and Peault, B.: Blood-forming potential of vascular endothelium in the human embryo, Development 129, 4147, 2002
Human, female 间皮 / Mesothelial Collagenase Type 1: 0.1%;
Deoxyribonuclease I: 0.05%
Eagle's MEM Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S: Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells, Hum Reprod 17, 2832-8, 2002
Human 干细胞,胚 / Stem, embryonic Neutral Protease: 0.01% - 0.02% DMEM Zhang, S., Wernig, M., Duncan, I., Brustle, O., and Thomson, J.: In Vitro Differentiation of Transplantable Neural Precursors from Human Embryonic Stem Cells, Nat Biotechnol 19, 1129, 2001
Human, 20-40 yr 子宫内膜上皮细胞和基质细胞 / Endometrium epithelial and stromal cells Collagenase Type 1: 0.2% HBSS Arnold, J., Kaufman, D., Seppala, M., and Lessey, B.: Endometrial stromal cells regulate epithelial cell grownth in vitro: a new co-culture model, Hum Reprod 16, 836, 2001
Human 绒毛 / Chorionic villi Trypsin: see reference;
Collagenase Type 3: 100 u/ml
HBSS Yusuf RZ, Naeem R: Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination, Early Pregnancy 5, 121-31, 2001
Human 子宫内膜内皮细胞 / Endometrial endothelial cells Collagenase Type 1: 0.2% McCoys medium Nikitenko LL, MacKenzie IZ,Rees MC, Bicknell R: Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium, Mol Hum Reprod 6, 811, 2000
Human, female, 20-40 year 微血管内皮细胞 / Microvascular endothelial cells Collagenase Type 2: 0.2%;
Deoxyribonuclease I: 0.0015%;
Trypsin: 0.05%
PBS Gargett CE, Bucak K, Rogers PA: Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells, Hum Reprod 15, 293-301, 2000
Human, female 子宫内膜上皮细胞 / Endometrial epithelial cells Collagenase Type 3: 45 u/ml;
Deoxyribonuclease I: .00035%
DMEM/F-12 Zhang J, Lathbury LJ, Salamonsen LA: Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium, Biol Reprod 62, 404-11, 2000
Human 膜细胞 / Theca cells Collagenase Type 1: 0.3%;
Deoxyribonuclease I: 0.0005%;
Hyaluronidase: 0.1%
PBS Runesson E, Ivarsson K, Janson P, Brannstrom M: Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle, J Clin Endocrinol Metab 85, 4387-95, 2000
Human 黄体细胞 / Corpus luteum cells Collagenase Type 2: 0.25%;
Deoxyribonuclease I: .005%
PBS Friden BE, Runesson E, Hahlin M, Brannstrom M: Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum, Mol Hum Reprod 6, 397-403, 2000
Human, females, 25-45 yr 黄体细胞 / Luteal cells Collagenase Type 2: 0.25% PBS Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M: Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture, Mol Hum Reprod 5, 714-9, 1999
Human, female, 25-41 year 卵泡 / Follicles Collagenase Type 2: 0.025-0.1% EBSS Hovatta O, Wright C, Krausz T, Hardy K, Winston RM: Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation, Hum Reprod 14, 2519-24, 1999
Human 胎盘 / Placental Deoxyribonuclease I: 0.04%;
Collagenase Type 2: 0.1%
PSS Bradbury., R., Sunn, K., Crossley, M., Bai, M., Brown, E., Delbridge, D., and Conigrave, A.: Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta, J Endocrinol 156, 425-430, 1998
Human, female 子宫内膜基质细胞 / Endometrial stromal cells Collagenase: 4000 u/ml DMEM/F-12 Huang JC, Liu DY, Dawood MY: The expression of vascular endothelial growth factor isoforms in cultured human endometrial stromal cells and its regulation by 17beta-oestradiol, Mol Hum Reprod 4, 603-7, 1998
Human 间质子宫内膜 / Stromal endometrial Collagenase Type 3: 45 u/ml;
Deoxyribonuclease I: .00035%
DMEM/Ham's F12 Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA: Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation, Biol Reprod 59, 693-703, 1998
Human 上皮,输卵管 / Epithelial, fallopian tube Collagenase Type 1: 1% Medium 199 Takeuchi, K., Maruyama, I., Yamamoto, S., Oki, T., Nagata, Y.: Isolation and Monolayer Culture of Human Fallopian Tube Epithelial Cells, In Vitro Cell Dev Biol 27, 720, 1991
Human 滋养细胞,胎盘 / Trophoblasts, placental Trypsin: 0.25% EBSS, CMF Branchaud, C.L., Goodyer, C.G., Guyda, H.J. and Lefebvre, Y.: A Serum-Free System for Culturing Human Placental Trophoblasts, In Vitro Cell Dev Biol 26, 865, 1990
Human 滋养细胞,胎盘 / Trophoblasts, placental Trypsin: 0.25% PBS Jie, Z., Fey, S., Hager, H., Hollsberg, P., Ebbesen, P., and Larsen, P.: Markers For Human Placental Trophoblasts in Two-Dimensional Gel Electrophoresis, In Vitro Cell Dev Biol 26, 937, 1990
Human 绒毛膜,胎盘 / Chorionic, placental Deoxyribonuclease I: 0.003% HBSS Egan, D., Grzegorczyk, V., Tricarico, K., Rueter, A.H., Olleman, W., and Marcotte, P.: Human Placental Chorionic Renin: Production, Purification and Characterization, Biochim Biophys Acta 965, 68, 1988
Human, female 子宫内膜 / Endometrial Collagenase: 2% RPMI 1640 Rinehart, C., Lyn-Cook, B., and Kaufman, D.: Gland formation from human endometrial epithelial cells in vitro, In Vitro Cell Dev Biol 24 (10), 1037, 1988
Human 胎盘 / Placental Trypsin: 0.25% DMEM Morrish, D., and Siy, O.: Critical Factors in Establishing Monolayer Cultures of Normal Human Placental Cells in Serum-Free Medium, Endocr Res 12 (3), 229, 1986
Human 内,黄体,子宫内膜,卵巢,子宫 / Interna & corpus luteum,Endometrium,Ovarian,Uterine Pronase: 0.1% Moscona's BSS Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984
Human, female, 27-49 years 上皮,卵巢 / Epithelial,Ovary Trypsin: 0.125% HBSS, CMF Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture, In Vitro 20, 743, 1984
Human 上皮,基质细胞 / Epithelial,Stromal Collagenase: 0.25% See reference Siegfried, J., Nelson, K., Martin, J., and Kaufman, D.: Histochemical Identification of Cultured Cells From Human Endometrium, In Vitro 20 (1), 25, 1984
Human, infant and neonate 上皮,前列腺 / Epithelial,Prostate Trypsin: 0.1% HBSS Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures, Methods Cell Biol 21, 195, 1980
Human, female, 27 years 平滑肌,子宫 / Smooth muscle, uterine Trypsin: 0.05% EBSS Rifas, L., Fant, J., Makman, M., and Seifter, S.: The Characterization of Human Uterine Smooth Muscle Cells in Culture, Cell Tissue Res 196, 385, 1979
Human 上皮,基质细胞 / Epithelial,Stromal Collagenase Type 1: 180 u/ml DMEM Kirk, D., King, R., Heyes, J., Peachey, L., Hirsch, P., and Taylor, W.: Normal Human Edometrium in Cell Culture, In Vitro 14 (8), 651, 1978
昆虫 Drosophila 卵巢 / Ovarian Collagenase Type 2: 0.1% Grace's Ma, X., Wang, S., Do, T., Song, X., Inaba, M., Nishimoto, Y., Liu, L., Gao, Y., Mao, Y., Li, H., McDowell, W., Park, J., Malanowski, K., Peak, A., Perera, A., Li, H. et al.: Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary., PLoS ONE 9, e90267, 2014
昆虫 Drosophila, embryonic 心鉴别 / Cardiac differentiating Collagenase Type 1: 20 u/ml;
Trypsin: 0.25%;
Deoxyribonuclease I: 4 u/ml
Schneider Salmand, P. Iche-Torres, M. and Perrin, L.: Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis.,Fly (Austin) Vol. 5,, , 261,
Rhesus macaque 卵泡 / Follicle Collagenase Type 1: 275 u/m;
Deoxyribonuclease I: 585 u/ml
HEPES Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB and Stouffer, RL: Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin., Hum Reprod 26, 1061-72, 2011
小鼠 Mouse, 4 week 滋养层 / Trophoblast Collagenase Type 1: 0.1%;
Collagenase Type 2: 0.25%
DMEM Hou, M., Han, J., Li, G., Kwon, M., Jiang, J., Emani, S., Taglauer, E., Park, J., Choi, E., Vodnala, M., Fong, Y., Emani, S., Rosas, I., Perrella, M. and Liu, X.: Multipotency of Mouse Trophoblast Stem Cells, Stem Cell Res Ther 11, 55, 2020
小鼠 Mouse, adult 睾丸 / Testes Collagenase Type 1: 120 u/ml;
Deoxyribonuclease I: 0.1%;
Trypsin: 5%
MEM Lee, A, Rusch, J., Lima, A., Usmani, A., Huang, N., Lepamets, M., Vigh-Conrad, K., Worthington, R., et al: Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility., Nat Commun 10, 4626, 2019
小鼠 Mouse, 1-7 day 睾丸 / Testicular Collagenase Type 4: 0.1%;
Deoxyribonuclease I: 0.1%
HBSS Kang, H., Chen, L., Lichti-Kaiser, K., Liao, G., Gerrish, K., Bortner, C., Yao, Hu., Eddy, E. and Jetten, A.: Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis., Stem Cells 34, 2772-2783, 2016
小鼠 Mouse, adult 精原干细胞 / Spermatogonial stem Trypsin: 0.05%;
Collagenase Type 1: 0.03%;
Deoxyribonuclease I: 80 u/ml
DMEM Martin, L. and Seandel, M.: Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines., J Vis Exp 72, e50017, 2013
小鼠 Mouse 卵巢 / Ovarian Collagenase Type 4: 800 u/ml HBSS White, Y., Woods, D., Takai, Y., Ishihara, O., Seki, H. and Tilly, J.: Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen., Nat Med 18, 413, 2012
小鼠 Mouse, female 淋巴细胞 / Lymphocytes Collagenase: 450 u/ml RPMI Jiang, J. and Kelly, K.: Isolation of Lymphocytes from Mouse Genital Tract Mucosa., J Vis Exp 67, e4391, 2012
小鼠 Mouse 阴道平滑肌 / Vaginal smooth muscle Collagenase Type 2: 175 u/ml;
Elastase: 0.025%
DMEM/F12 Venkataraman, L., Lenis, A., Couri, B., Damaser, M. and Ramarurthi, A: Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse, J Tissue Sci Eng 3, 3, 2012
小鼠 Mouse, male 睾丸,减数分裂 / Testis, meiotic Collagenase Type 1: 120 u/ml;
Deoxyribonuclease I: 0.001%;
Trypsin: 0.1%
Gey's BSS Getun, I., Torres, B. and Bois, P.: Flow Cytometry Purification of Mouse Meiotic Cells., J Vis Exp 50, 2602, 2011
小鼠 Mouse 透明带, 无卵细胞 / Zona pellucid free eggs Collagenase: 0.1% THY-HEPES Yamatoya, K., Ito, C., Araki, M., Furuse, R. and Toshimori, K.: One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation., Reprod Med Bio 10, 97-103, 2011
小鼠 Mouse 子宫造口 / Uterine stomal Neutral Protease: 0.6%;
Pancreatin: 0.25%;
Collagenase Type 3: 0.05%
DMEM/F12 Chen, L., Belton, R. and Nowak, R.: Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation., Endocrinology 150, 966, 2009
小鼠 Mouse 生殖细胞 / Germ cells Collagenase Type 1: 100 u/ml HBSS Breault, D., Min, I., Carlone, D., Farilla, L., Ambruzs, D., Henderson, D., Algra, S., Montgomery, R., Wagers, A. and Hole, N.: Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells., Proc Natl Acad Sci U S A 105, 10420, 2008
小鼠 Mouse, embryonic 造血干细胞 / Hematopoietic stem cells Collagenase: 0.1% PBS Gekas, C., Rhodes, K. and Mikkola, H.: Isolation and Analysis of Hematopoietic Stem Cells from the Placenta., J Vis Exp 16, e742, 2008
小鼠 Mouse, 6 day 塞尔托利 / Sertoli Collagenase Type 2: 500 u/ml;
Hyaluronidase: 0.1%;
Deoxyribonuclease I: 0.0005%
DMEM Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta: Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse, Biol Reprod 69, 985-94, 2003
小鼠 Mouse, adult, neonatal and fetal 睾丸细胞 / Testicular cells Collagenase Type 1: 0.1% DMEM/F-12 O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ: Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis, Endocrinology 144, 3279-84, 2003
小鼠 Mouse, male, 3.5 months old 睾丸间质 / Leydig Collagenase: 0.06% Medium E 199 Faldikova, L., Diblikova, I., Canderle, J., Zraly, Z., Veznik, Z., and Sulcova, A.: Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production, Vet Med 46 (6), 160, 2001
小鼠 Mouse, male, 12-14-week-old 曲细精管 / Seminiferous tubules Trypsin: 0.05% DMEM Lin, Q., Sirotkin, A., and Skoultchi, A.: Normal Spermatogenesis in Mice Lacking the Testis-Specific Linker Histone H1t, Mol Cell Biol 20 (6), 2122, 2000
小鼠 Mouse, 6-12 day 卵母细胞-颗粒 / Oocyte-granulosa Collagenase Type 1: 0.1%;
Deoxyribonuclease I: 0.02%
Waymouth Eppig JJ, O'Brien MJ: Development in vitro of mouse oocytes from primordial follicles, Biol Reprod 54, 197-207, 1996
小鼠 Mouse, female,6-8 weeks 子宫 / Uterine Trypsin: 0.2% HBSS Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes, In Vitro Cell Dev Biol 27A, 713, 1991
小鼠 Mouse, female, 21 day, 10-12 g 阴道上皮 / vaginal epithelial Collagenase Type 3: 38 u/ml DMEM Tsai, P., Uchima, F., Hamamoto, S., and Bern, H.: Proliferation and Differentiation of Prepubertal Mouse Vaginal Epithelial Cells In Vitro and the Specificity of Estrogen-Induced Growth Retardation, In Vitro Cell Dev Biol 27A, 461, 1991
小鼠 Mouse, CF1, female 积云,单细胞胚胎 / Cumulus, one-cell embryos Hyaluronidase: 0.1% PBS, CMF Spindle, A.: In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition, In Vitro Cell Dev Biol 26, 151, 1990
小鼠 Mouse (BALB/cCRGL), male, 2-3 months 上皮,前列腺 / Epithelial, prostate gland Hyaluronidase: 0.1% Medium 199 Turner, T., Bern, H., Young,P., and Cunha, G.: Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel, In Vitro Cell Dev Biol 26, 722, 1990
小鼠 Mouse (C57B/T), fetus, 16-17 days old 前列腺 / Prostate Trypsin: 1.0% HBSS/ DMEM Thompson, T.C., Southgate, J., Kitchener, G., and Land, H.: Multistage Carcinogenesis Induced by ras and myc Oncogenes in a Reconstituted Organ, Cell 56, 917, 1989
小鼠 Mouse, outbred, CD-1, 21-23 days old 上皮 / Epithelial Trypsin: 0.5% Medium 199 Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro, Endocrinology 118 (3), 1011, 1986
小鼠 Mouse, BALB/c 上皮,间质 / Epithelial,Mesencymal Trypsin: 1% DMEM Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells, Am J Physiol 251, E630, 1986
小鼠 Mouse, BALB/cCrg, 40 days old 上皮 / Epithelial Trypsin: 1% Medium 199 Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma, Proc Natl Acad Sci U S A 83, 2109, 1986
小鼠 Mouse, male, 10-13 wk 睾丸,睾丸 / Leydig,Testis Deoxyribonuclease I: 0.001% Medium 199 w/ BSA Stalvcey, J. and Payne, A.: Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains, Endocrinology 112, 1696, 1983
小鼠 Mouse (BALB/cCrgl), female, 50 - 60 days 上皮,阴道 / Epithelial, vagina Collagenase Type 3: 0.1% HBSS Iguchi, T., Uchima, F.A., Ostrander, P., and Bern, H.: Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels, Proc Natl Acad Sci U S A 80, 3743, 1983
绵羊 Ovine, 3-5 year 子宫内膜间质 / Endometrial stromal Collagenase Type 1: 0.5%;
Deoxyribonuclease I: 0.04 u/ml
DMEM/F-12 Letouzey, V., Tan, K., Deane, J., Ulrich, D., Gurung, S., Ong, Y. and Gargett, C.: Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium., PLoS ONE 10, e0127531, 2015
绵羊 Sheep 上皮 / Epithelial Collagenase: 125 - 190 u/ml DMEM Salamonsen, L., Sum O, W., Doughton, B., and Findlay, J.: The Effects of Estrogen and Progesterone In Vivo on Protein Synthesis and Secretion by Cultured Epithelial Cells from Sheep Endometrium, Endocrinology 117 (5), 2148, 1985
Porcine, 1-4 day 睾丸 / Testicular Collagenase Type 4: 0.1%;
Hyaluronidase: 0.1%;
Trypsin: 0.25%
DMEM Park, M., Park, J., Kim, M., Lee, K., Park, H., Yun, J., Choi, J., Lee, E. and Lee, S.: Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes., J Assist Reprod Genet 31, 983-91, 2014
Porcine, male, 8 day 生精上皮细胞 / Seminiferous epithelial cells Collagenase: 0.15%;

Deoxyribonuclease I: .0001%;

Hyaluronidase: 0.15%;

Trypsin: 0.05%;

DMEM/F12 Dirami G, Ravindranath N, Pursel V, Dym M: Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM, Biol Reprod 61, 225-30, 1999
Porcine, female 黄体 / Corpus Leuteum Collagenase Type 4: 600 u/ml Medium 199 Ciereszko, R., Petroff, B., Ottobre, A., Guan, Z., Stokes, B., and Ottobre, J.: Assessment of the Mechanism by Which Prolactin Stimulates Progesterone Production by Early Corpora Lutea of Pigs, J Endocrinol 159, 201, 1998
Porcine (also bovine, human) 内黄体, 子宫内膜, 卵巢, 子宫, / Interna & corpus luteum,
Endometrium,
Ovarian,
Uterine,
Pronase: 0.1% Moscona's BSS Marcus, G., Connor, L., Domingo, M., Tsang, B., Downey, B., and Ainsworth, L.: Enzymatic Dissociation of Ovarian and Uterine Tissues, Endocr Res 10, 151, 1984
Porcine, 3-4 week 睾丸,睾丸 / Leydig,Testis Trypsin: 0.0003% Lebovitz L-15 Medium Mather, J., Saez, J., and Haour, F.: Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells, Endocrinology 110, 933, 1982
Rabbit, New Zealand, 3 month 睾丸细菌 / Testicular germ Collagenase Type 1: 0.1%;
Trypsin: 0.25%;
Deoxyribonuclease I: 0.7%
HBSS Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties., FASEB J 25, 2604, 2011
Rabbit, New Zealand, 3 month 睾丸细菌 / Testicular germ Collagenase Type 1: 0.1%;
Trypsin: 0.25%;
Deoxyribonuclease I: 0.7%
HBSS Kubota, H., Wu, X., Goodyear, S., Avarbock, M. and Brinster, R.: Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties., FASEB J 25, 2604, 2011
Rabbit, New Zealand white, 4-6 month old, 卵巢,间皮 / Ovarian,Mesothelial Collagenase Type 1: 300 u/ml HBSS Setrakian, S., Oliveros-Saunders, B., and Nicosia, S.: Growth Stimulation of Ovarian and Extraovarian Mesothelial Cells by Corpus Luteum Extract, In Vitro Cell Dev Biol 29A, 879, 1993
Rabbit, New Zealand white, adult, female (nonpregnant), 肌细胞,子宫 / Myocytes, uterine Deoxyribonuclease I: 200 µg/ml HBSS-HEPES buffer Phillippe, M., Saunders, T., and Bangalore, S.: Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes, In Vitro Cell Dev Biol 26, 369, 1990
Rabbit, New Zealand, mature, female, 4-5 Kg 卵巢表面上皮和腹膜间皮 / Ovarian surface epithelial and peritoneal mesothelial Collagenase Type 1: 300 IU/ml (280 IU/mg) HBSS, CMF Piquette, G., and Timms, B.: Isolation and Characterization of Rabbit Ovarian Surface Epithelium, Granulosa Cells, and Peritoneal Mesothelium in Primary Culture, In Vitro Cell Dev Biol 26, 471, 1990
Rabbit, mixed breed 子宫内膜上皮 / Endometrial epithelial Collagenase Type 1: 0.005% DMEM Mulholland, J., Winterhager, E., and Beier, H.: Changes in Proteins Synthesized by Rabbit Endometrial Epithelial Cells Following Primary Culture, Cell Tissue Res 252, 123, 1988
Rabbit, New Zealand white, mature, 3-4 Kg 子宫肌层 / Myometrial Trypsin: 0.02%, 0.03%, 0.0375% HBSS Boulet, A., and Fortier, M.: Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment, In Vitro Cell Dev Biol 23, 93, 1987
Rabbit, New Zealand white estrous, female, 4-5 months 间皮和表面上皮,卵巢 / Mesothelial and surface epithelial,Ovaries Trypsin: 0.125%-0.5% Medium 199 Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium), Int J Gynecol Pathol 3, 348, 1984
大鼠 Rat 胎盘间充质 / Placental mesenchymal Collagenase Type 4: 0.1%;
Deoxyribonuclease I: 0.02%;
Neutral Protease: 0.1%
DMEM Jiang, H., Zhang, Y., Tian, K., Wang, B. and Han, S.: Amelioration of Experimental Autoimmune Encephalomyelitis Through Transplantation of Placental Derived Mesenchymal Stem Cells., Sci Rep 7, 41837, 2017
大鼠 Rat, SD, 350-450 g 睾丸间质 / Leydig cells Collagenase: .05-0.1% Medium 199 Sharma RS, Pal PC, Rajalakshmi M.: Isolation and Culture of Leydig Cells from Adult Rats, Indian J Clinical Biochem 21, 27, 2006
大鼠 Rat, SD, adult, male, 8 - 10 week 曲细精管 / Seminiferous tubules Trypsin: 0.05% Krebs-Ringer bicarbonate buffer Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization, Biol Proced Online 3 (1), 35, 2001
大鼠 Rat, SD, immature 子房 / Ovary Collagenase Type 1: 144 u/ml McCoy's 5a Ando, M., Kol, S., Irahara, M., Sirois, J., and Adashi, E.: Non-Steroidal Anti-Inflammatory Drugs (NSAIDS) Block the Late, Prostanoid-Dependent/Ceramide-Independent Component of Ovarian IL-1 Action: Implications for the Ovulatory Process, Mol Cell Endocrinol 157, 21, 1999
大鼠 Rat, Han-Wistar, adult, male, 250 g 睾丸 / Testes Trypsin: 0.1% Medium 199 w/ Hank's salts Leckie, C., Welberg, L., and Seckl, J.: 11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells, J Endocrinol 159, 233, 1998
大鼠 Rat, SD, female, immature, 26 day 黄体 / Corpus luteum Neutral Protease: 2.4 u/ml Deoxyribonuclease: 200u/ml Serum-free medium Tellieria, C., Ou, J., Sugino, N., Ferguson, S., and Gibori, G.: The Expression of Interleukin-6 in the Pregnant Rat Corpus Luteum and Its Regulation by Progesterone and Glucocorticoid, Endocrinology 139 (8), 3597, 1998
大鼠 Rat, SD, 25 day 卵巢膜间质 / Ovarian theca-interstitial Collagenase Type 1: 0.5%;
Deoxyribonuclease I: 0.02%
Medium 199 Duleba AJ, Spaczynski RZ, Olive DL, Behrman HR: Effects of insulin and insulin-like growth factors on proliferation of rat ovarian theca-interstitial cells, Biol Reprod 56, 891-7, 1997
大鼠 Rat, SD, male, 90 days 塞尔托利 / Sertoli Hyaluronidase: 0.1% DMEM Onoda, M. and Djakiew, D.: Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin, In Vitro Cell Dev Biol 27, 215, 1991
大鼠 Rat, SD, male 睾丸间质 / Leydig Trypsin: 0.02% DMEM Abayasekara, D., Kurlak, L., Band, A., Sullivan, M., and Cooke, B.: Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis, In Vitro Cell Dev Biol 27, 253, 1991
大鼠 Rat, neonatal 子宫 / Uterine Trypsin: 1% HBSS Branham, W., Lyn-Cook, B., Andrews, A., McDaniel, M., Sheehan, D.: Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix, In Vitro Cell Dev Biol 27, 442, 1991
大鼠 Rat, SD, male, 120-160 g Leydig,肾上腺 / Leydig,Adrenal Collagenase Type 2: 0.03% (adrenal) Krebs Ringer bicarbonate buffer Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26, 24, 1990
大鼠 Rat, Wistar, adult 子宫 / Uterine Trypsin: 0.5% PBS Pampfer, S., Vanderheyden, I., Michiels, B., and DeHertogh, R.: Co-Culture of Two-Cell Rat Embryos on Cell Monolayers, In Vitro Cell Dev Biol 26, 944, 1990
大鼠 Rat, SD, 10 day 支持细胞 / Sertoli cells Collagenase Type 2: 500 u/ml;
Deoxyribonuclease I: 0.0005%;
Hyaluronidase: 0.1%
PBS Hadley MA, Weeks BS, Kleinman HK, Dym M: Laminin promotes formation of cord-like structures by Sertoli cells in vitro, Dev Biol 140, 318-27, 1990
大鼠 Rat, immature 黄体,卵巢 / Luteal, ovaries Collagenase: 0.3% McCoy's Rajan, V. and Menon, K.: Differential Uptake and Metabolism of Free and Esterified Cholesterol from High-density Lipoproteins in the Ovary, Biochim Biophys Acta 959, 206, 1988
大鼠 Rat, male, 20 day 塞尔托利 / Sertoli Trypsin: 0.15% (see reference) Skinner, M., Fetterolf, P., and Anthony, C.: Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function, J Biol Chem 263, 2884, 1988
大鼠 Rat, SD, female 管腔上皮 / Luminal epithelial Trypsin: 0.5% HBSS Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells, J Cell Biol 107 (6), 2409, 1988
大鼠 Rat, Wistar, virgin, female, 250 g 阴道上皮 / Vaginal epithelial Trypsin: 0.5% PBS Conti, C., and Tasat, D.: Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone, J Steroid Biochem 24 (3), 747, 1986
大鼠 Rat, SD, female, 21 day 黄体,卵巢 / Luteal, ovaries Deoxyribonuclease I: 0.0004% McCoy's Rajan, V. and Menon, K.: Involvement of Microtubules in Lipoprotein Degradation and Utilization for Steroidogenesis in Cultured Rat Luteal Cells, Endocrinology 117, 2408, 1985
大鼠 Rat, SD, male, 10 day 塞尔托利 / Sertoli Trypsin: 0.025% DMEM Hadley, M., Byers, S., Suarez-Quian, C., Kleinman, H., and Dym, M.: Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro, J Cell Biol 101, 1511, 1985
大鼠 Rat, Holtzman, female, immature 子宫 / Uterine Deoxyribonuclease I: 0.025% DMEM buffered with HEPES Kassis, J., Walent, J., and Gorski, J.: Estrogen Receptors in Rat Uterine Cell Cultures: Effects of Medium on Receptor Concentration, Endocrinology 115, 762, 1984
大鼠 Rat, SD, adult,male, 50-70 days 睾丸 / Testicular Deoxyribonuclease I: 10 µg/ml HEPES Hsueh, A., Bambino, T., Zhuang, L., Welsh, T., and Ling, N.: Mechanism of the Direct Action of Gonadotropin Releasing Hormone and Its Antagonist on Androgen Biosynthesis by Cultured Rat Testicular Cells, Endocrinology 112, 1653, 1983
大鼠 Rat, SD, female, pseudopregnant, 21 day 黄体,卵巢 / Luteal, ovaries Hyaluronidase: 0.1% EBSS Rajendran, K., Hwang, J., and Menon, K.: Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells, Endocrinology 112, 1746, 1983
大鼠 Rat, SD, 4-6 day, male Sertolli,曲细小管 / Sertolli, seminiforous tubules Collagenase: 0.03% Serum-free medium Rich, K., Bardin, C., Gunsalus, G., and Mather, J.: Age-Dependent Pattern of Androgen-Binding Protein Secretion from Rat Sertoli Cells in Primary Culture, Endocrinology 113, 2284, 1983
大鼠 Rat, SD, female, 26 days 黄体 / Luteal Deoxyribonuclease I: 0.0004% Medium 199 Azhar, S. and Reaven, E.: Effect of Antimicrotubule Agents on Microtubules and Steroidogenesis in Luteal Cells, Am J Physiol 243, E380, 1982
大鼠 Rat, SD, male 睾丸间质 / Leydig Collagenase: 0.1% Krebs Ringer bicarbonate buffer Ramachandran, J., and Sairam, M.R.: The Effects Of Interstitial Cell-Stimulating Hormone, Its Subunits,and Recombinants on Isolated Rat Leydig Cells, Arch Biochem Biophys 167, 294, 1975
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