原代细胞分离中，针对腮腺组织涉及到腮腺和舌下腺，腺泡、腮腺腺泡细胞、上皮，腺泡，眶外泪腺，腮腺，胰腺等等的解离通常会使用酶，如何选择合适的酶来完成对应腮腺组织的解离，以及原代细胞采集的常规操作， 艾美捷针对腮腺相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
| 腮腺 | |||||
| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 小鼠 | Mouse | 腮腺和舌下腺 / Parotid and sublingual glandular | Neutral Protease: 50 u/ml； Collagenase Type 1: 100 u/ml |
PBS | Ogawa, M., Oshima, M., Imamura, A., Sekine, Y., Ishida, K., Yamashita, K., Nakajima, K., Hirayama, M., Tachikawa, T. and Tsuji, T.: Functional Salivary Gland Regeneration by Transplantation of a Bioengineered Organ Germ., Nat Commun 4, 2498, 2013 |
| 大鼠 | Rat, Wistar, male, 130 g | 腺泡 / Acinar | Collagenase: 75 u/ml； Hyaluronidase: 153 u/ml |
RPMI 1640 | Looms, D., Dissing, S., Tritsaris, K., Pedersen, A. and Nauntofte, B.: Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells., Adv Dent Res 14, 62, 2000 |
| 大鼠 | Rat, SD, 100-120 g | 腮腺腺泡细胞 / Parotid acinar cells | Collagenase Type 2: 90 u/ml | Krebs-Henseleit Bicarbonate | D'Silva NJ, DiJulio DH, Belton CM, Jacobson KL, Watson EL: Immunolocalization of Rap1 in the rat parotid gland: detection on secretory granule membranes, J Histochem Cytochem 45, 965-73, 1997 |
| 大鼠 | Rat, SD, male | 上皮 / Epithelial | Collagenase: 50-75 u/ml； Hyaluronidase: 0.1% |
HBSS CF | Prasad, K.N., Edwards-Prasad, J., Carvaiho, I., LaRosa, F.G., Baibinder, E., Meyers, A., and Quissell, D.: Establishment of Primary Cultures of Rat and Human Parotid Epithelial Cells for Transfection Experiments, In Vitro Cell Dev Biol 28, 493, 1992 |
| 大鼠 | Rat, SD, male, 100-150 g | 腮腺腺泡 / Parotid acinar | Trypsin: 0.001% | F12 medium | Yeh, C., Mertz, P., Oliver, C., Baum, B., and Kousvelari, E.: Cellular Characteristics of Long-Term Cultured Rat Parotid Acinar Cells, In Vitro Cell Dev Biol 27, 707, 1991 |
| 大鼠 | Rat, Wistar, male | 腮腺腺泡 / Parotid acinar | Trypsin: 0.02% | Solution B (See reference) | Foskett, J., Roifman, C. and Wong, D.: Activation of Calcium Oscillations by Thapsigargin in Parotid Acinar Cells, J Biol Chem 266, 2778, 1991 |
| 大鼠 | Rat | 腮腺 / Parotid | Hyaluronidase: 0.025% | HBSS with 20mM HEPES | Takuma, T. and Ichida, T.: Amylase Secretion From Saponin-Permeabilized Parotid Cells Evoked by Cyclic AMP, J Invest Dermatol 103, 95, 1988 |
| 大鼠 | Rat, Wistar, male, 250 - 350 g | 腺泡，腮腺 / Acinar, parotid | Hyaluronidase: 0.015% | Earle's MEM | Melvin, J., Kawaguchi, M., Baum, B., and Turner, R.: A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells, Biochem Biophys Res Commun 145, 754, 1987 |
| 大鼠 | Rat, SD, male, 50 - 125 g | 腺泡，眶外泪腺，腮腺，胰腺 / Acinar，Exorbital lacrimal, parotid, pancreas | Trypsin: 0.01% | HBSS, CMF | Oliver, C., Waters, J., Tolbert, C., and Kleinman, H.: Growth of Exocrine Acinar Cells on a Reconstituted Basement Membrane Gel, In Vitro Cell Dev Biol 23, 465, 1987 |
| 大鼠 | Rat, SD, albino, male, 150-200 g | 腺泡，腮腺 / Acinar, parotid | Trypsin: 0.01%； Collagenase: 40-50 u/ml； Hyaluronidase: 0.10% |
HBSS CMF | Mangos, J., McSherry, N., Butcher, F., Irwin, K., and Barber, T.: Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character), Am J Physiol 229 (3), 560, 1975 |
