原代细胞分离中，针对神经系统组织涉及到中肠DRG，睫状神经节神经元、心脏，肾上腺嗜铬，副神经元、卫星胶质等等的解离通常会使用酶，如何选择合适的酶来完成对应神经系统组织的解离，以及原代细胞采集的常规操作， 艾美捷针对神经系统相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
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| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 禽类 | Quail and Chicken, embryonic | 中肠DRG / Midgut DRG | Collagenase animal free: 0.05%； Neutral Protease: 0.5% |
Ham's F12 | Rollo, B., Zhang, D., Simkin, J., Menheniott, T. and Newgreen, D.: Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells., F1000Res 4, 113, 2015 |
| 禽类 | Chick, White Leghorn, embryos | 睫状神经节神经元 / Cilary ganglion neurons | Collagenase Type 1: 0.1% | PBS | Temburni, M., Rosenberg, M., Pathak, N., McConnell, R. and Jacob, M.: Neuronal Nicotinic Synapse Assembly Requires the Adenomatous Polyposis Coli Tumor Suppressor Protein., J Neurosci 24, 6776, 2004 |
| 牛 | Bovine | 心脏，肾上腺嗜铬，副神经元 / Heart，Adrenal chromaffin，Paraneurons | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| 牛 | Bovine | 微血管内皮 / Microvascular endothelial | Collagenase/Dispase: 0.1% | MEM | Bowman, P., Betz, A., and Goldstein, G.: Primary Culture of Microvascular Endothelial Cells From Bovine Retina, In Vitro 18 (7), 626, 1982 |
| 牛 | Calf | 少突胶质细胞，神经 / Oligodendroglia，Neural | Trypsin: 0.1% | (see reference) | Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978 |
| 犬/狗 | Canine | 卫星胶质 / Satellite glial | Hyaluronidase: 0.2%； Collagenase Type 4: 0.2%； Collagenase: 0.2% |
HBSS | Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgartner, W. and Gerhauser, I.: Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties., Sci Rep 7, 13915, 2017 |
| 鸡 | Chick, White Leghorn, embryos | 胰岛 / Islets | Trypsin: Trypsin: 0.125-0.25%； Collagenase: 0.2%； Deoxyribonuclease I: 200 u/ml |
Eurocollins solution | Watt, P., Mullen, Y., Benhamou, P., Hober, C., Nomura, Y., Watanabe, Y., Passaro, E., Zinner, M., and Brunicardi, F.: Simplified Semiautomated Method For Isolating Islets From the Human Pancreas, Transplant Proc 26 (2), 582, 1994 |
| 鸡 | Chicken, White Leghorn, embryos, 17-21 day | 神经元 / Neurons | Papain: 40 u/ml | HEPES | Raman, I., and Trussell, L.: The Kinetics of the Response to Glutamate and Kainte in Neurons of the Avian and Cochlear Nucleus, Neuron 9, 173, 1992 |
| 鸡 | Chick, embryo, 10-14 day | 平坦，视网膜 / Flat, retina | Trypsin: 0.1% | Tyrode's solution, CMF | Moyer, M., Bullrich, F., and Sheffield, J.: Emergence of Flat Cells From Glia in Stationary Cultures of Embryonic Chick Neural Retina, In Vitro Cell Dev Biol 26, 1073, 1990 |
| 鸡 | Chick, White Leghorn, embryo | 脊髓 / Spinal cord | Trypsin: 0.05% | Phosphate buffer | Schnaar, R., and Schaffner, A.: Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions, J Neurosci 1, 204, 1981 |
| 鸡 | Chick | 背根神经节神经元，脊髓 / Dorsal root ganglion neurons，Spinal cord | Trypsin: 0.1% | Puck's saline, CMF | Choi, D., and Fiscbach, G.: GABA Conductance of Chick Spinal Cord and Dorsal Root Ganglion Neurons in Cell Culture, J Neurophysiol 45, 605, 1981 |
| 鸡 | Chick, White Leghorn embryo, 8 day | 神经元，神经节 / Neurons, ganglia | Trypsin: 0.25% | HBSS, CMF | Bottenstein, J., Skaper, S., Varon, S., and Sato, G.: Selective Survival of Neurons from Chick Embryo Sensory Ganglionic Dissociates Utilizing Serum-Free Supplemented Medium, Exp Cell Res 125, 183, 1980 |
| 鸡 | Chicken, White Leghorn, fertile eggs | 睫状神经节神经元 / Ciliary ganglion neurons | Trypsin: 0.25% | Eagle's MEM | Tuttle, J., Suszkiw, J., and Ard, M.: Long-Term Survival and Development of Dissociated Parasympathetic Neurons in Culture, Brain Res 183, 161, 1980 |
| 鸡 | Chicken, embryos, 9-10 days old | 背根神经节神经元 / Dorsal root ganglia neurons | Collagenase: 0.01% | Eagle's MEM | Mudge, A., Leeman, S., and Fischbach, G.: Enkephalin Inhibits Release of Substance P From Sensory Neurons in Culture and Decreases Action Potential Duration, Proc Natl Acad Sci U S A 76 (1), 526, 1979 |
| 鸡 | Chick embryos | 神经节链，交感神经节 / Ganglion chains, sympathetic ganglia | Trypsin: 0.25% | Krebs Phosphosaline | McCarthy, K., and Partlow, L.: Preparation of Pure Neuronal and Non-Neuronal Cultures From Embryonic Chick Sympathetic Ganglia: A New Method Based on Both Differential Cell Adhesiveness and the Formation of Homotypic Neuronal Aggregates, Brain Res 114, 391, 1976 |
| 鸡 | Chick, White Leghorn, embryos, 7 day | 神经视网膜 / Neural retina | Collagenase: 0.25%；
Elastase: 0.2%； Hyaluronidase: 1.0%； Papain: 1.0%； Protease: 0.1%； Trypsin: 0.05%； |
HBSS | Wiseman, L., and Hammond, W.: The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents, J Exp Zool 197, 429, 1976 |
| 鱼 | Zebrafish, embryonic | 运动神经元 / Motorneurons | PDS kit: per instructions | L-15 | Spiro, Z., Koh, A., Tay, S., See, K. and Winkler, C.: Transcriptional Enhancement of Smn Levels in Motoneurons is Crucial for Proper Axon Morphology in Zebrafish., Sci Rep 6, 27470, 2016 |
| 鱼 | Zebrafish | 运动神经元 / Motor neurons | Collagenase Type 2: 0.1% | See reference | Sakowski, S., Lunn, J., Busta, A., Palmer, M., Dowling, J. and Feldman, E.: A Novel Approach to Study Motor Neurons from Zebrafish Embryos and Larvae in Culture., J Neurosci Methods 205, 277-82, 2012 |
| 鱼 | Zebrafish, Dania rerio, embryo | Rohon-Beard 神经元 / Rohon-Beard neurons | Trypsin: 0.2% | L-15/Hepes | Won, Y., Ono, F. and Ikeda, S.: Identification and Modulation of Voltage-Gated Ca2+ Currents in Zebrafish Rohon-Beard Neurons., J Neurophysiol 105, 442-53, 2011 |
| 鱼 | Zebrafish, Danio rerio, embryo | 神经元 / Neurons | PDS kit: with modifications | EBSS | Cerda, G., Hargrave, M. and Lewis, K.: RNA Profiling of FAC-sorted Neurons from the Developing Zebrafish Spinal Cord., Dev Dyn 238, 150-61, 2009 |
| 鱼 | Black ghose knige fish, adult (Apteronotus albifrons) | 神经元，脊髓 / Neurons, spinal cord | Trypsin: 0.4% | PBS, CMF | Anderson, M.J.: Differences in Growth of Neurons from Normal and Regenerated Teleost Spinal Cord in vitro, In Vitro Cell Dev Biol 29A, 145, 1993 |
| 青蛙 | Xenopus, embryonic | 神经元 / Neuron | Collagenase Type 1: 0.1% | Steinberg's solution | Takahashi, T., Nakajima, Y., Hirosawa, K., Nakajima, S., and Onodera, K.: Structure and Physiology of Developing Neuromuscular Synapses in Culture, J Neurosci 7, 473, 1987 |
| 豚鼠 | Guinea-pig, newborn | 神经元，肠 / Neuron, enteric | Trypsin: 0.125% | Medium 199 | Jessen, K, McConnell, J., Purves, R., Burnstock, G., and Chamley-Campbell, J.: Tissue Culture of Mammalian Enteric Neurons, Brain Res 152, 573, 1978 |
| 仓鼠 | Hamster, male | 犁鼻器神经元 / Vomeronasal organ neurons | Collagenase Type 1: 0.02%； Trypsin: 0.02% |
PBS | Liman ER: Regulation by voltage and adenine nucleotides of a Ca2+-activated cation channel from hamster vomeronasal sensory neurons, J Physiol 548, 777, 2003 |
| 人 | Human | 脊髓神经祖细胞 / Spinal cord neural progenitor | PDS kit: with modifications | EBSS | Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015 |
| 人 | Human | 神经的，各种 / Neural, various | Papain: 12 u/ml； Trypsin: see reference； Collagenase/Dispase: see reference |
see reference | Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007 |
| 人 | Human, 9 month | 神经元 / Neurons | Collagenase Type 4: 1.33%； Papain: 0.07 u/ml； Neutral Protease: 1 mg/ml |
DMEM/F12 | Dietrich J, Lacagnina M, Gass D, Richfield E, Mayer-Proschel M, Noble M, Torres C, Proschel C.: EIF2B5 mutations compromise GFAP+ astrocyte generation in vanishing white matter leukodystrophy, Nat Med 11, 277, 2005 |
| 人 | Human, 26 week | 神经祖细胞 / Neural progenitor cells | Papain: 2.5 u/ml； Deoxyribonuclease I: 250 u/ml； Neutral Protease: 1 u/ml |
DMEM/F-12 | Fuja TJ, Schwartz PH, Darcy D, Bryant PJ.: Asymmetric localization of LGN but not AGS3, two homologs of Drosophila pins, in dividing human neural progenitor cells, J Neurosci Res 75, 782, 2004 |
| 人 | Human, adult | 心室上皮 / Ventricular epithelial | Papain: 11.4 u/ml； Deoxyribonuclease I: 10 u/ml |
DMEM/F12 | Roy NS, Benraiss A, Wang S, Fraser RA, Goodman R, Couldwell WT, Nedergaard M, Kawaguchi A, Okano H, Goldman SA: Promoter-targeted selection and isolation of neural progenitor cells from the adult human ventricular zone, J Neurosci Res 59, 321, 2000 |
| 人 | Human, 5-65 years | 视网膜色素上皮 (RPE) / Retinal pigment epithelial (RPE) | Trypsin: 0.25% | HBSS | Von Recum, H., Okano, T., Kim, S, and Bernstein, P.: Maintenance of Retinoid Metabolism in Human Retinal Pigment Epithelium Cell Culture, Exp Eye Res 69, 97, 1999 |
| 人 | Human | 背根神经节神经元 / Dorsal root ganglion neurons | Collagenase Type 1: 0.2%； Neutral Protease: 0.5% |
DMEM/F-12 | Dib-Hajj SD, Tyrrell L, Cummins TR, Black JA, Wood PM, Waxman SG.: Two tetrodotoxin-resistant sodium channels in human dorsal root ganglion neurons, F.E.B.S. Lett. 462, 117, 1999 |
| 昆虫 | Drosophila, larval | 神经母细胞 / Neuroblasts | Collagenase Type 1: 0.1%； Papain: 0.1% |
Rinaldini solution | Berger, C., Harzer, H., Burkard, T., Steinmann, J., van der Horst, S., Laurenson, A., Novatchkova, M., Reichert, H. and Knoblich, J.: FACS Purification and Transcriptome Analysis of Drosophila Neural Stem Cells Reveals a Role for Klumpfuss in Self-Renewal., Cell Rep 2, 407, 2012 |
| 昆虫 | Drosophila | 树突 / Dendrites | Collagenase: 0.05%； Neutral Protease: 0.2% |
HBSS | Sanchez-Soriano, N., Bottenberg, W., Fiala, A., Haessler, U., Kerassoviti, A., Knust, E., Lohr, R. and Prokop, A.: Are Dendrites in Drosophila Homologous to Vertebrate Dendrites?, Dev Biol 288, 126, 2005 |
| 昆虫 | Gryllus Bimaculatus | 巨大的中间神经元 / Giant interneurons | Collagenase: 0.05%； Neutral Protease: 0.2% |
Leibovitz's L15 | Kloppenburg, P. and Horner, M.: Voltage-Activated Currents in Identified Giant Interneurons Isolated from Adult Crickets Gryllus Bimaculatus, J Exp Biol 201 (Pt 17), 2529, 1998 |
| 猴 | Macaque | 背根神经节 / Dorsal root ganglia | Papain: 35 u/ml； Deoxyribonuclease I: 80 u/ml； Stemxyme 1: 0.2% |
see reference | Kupari, J., Usoskin, D., Parisien, M., Lou, D., Hu, Y., Fatt, M., Lonnerberg, P., Spangberg, M., Eriksson, B., Barkas, N., Kharchenko, P., Lore, K, Khoury, S., Diatchenko, L. and Ernfors, P.: Single Cell Transcriptomics of Primate Sensory Neurons Identifies Cell Types Associated with Chronic Pain., Nat Commun 12, 1510, 2021 |
| 小鼠 | Mouse | 背根神经节 / Dorsal root ganglion | Stemxyme 1: 0.1-0.2% | HBSS | Yousuf, M., Samtleben, S., Lamothe, S., Friedman, T., Catuneanu, A., et al.: ER Stress in the Dorsal Root Ganglia Regulates BK Channel Physiology and Contributes to Pain Hypersensitivity in a Mouse Model of Multiple Schlerosis, bioRxivorg , , 2020 |
| 小鼠 | Mouse, embryonic 12.5 | 雪旺细胞前体 / Schwann cell precursors | Collagenase Type 2: 0.2%；
Hyaluronidase: 0.12%； Soybean Trypsin Inhibitor: 0.03%； |
DMEM/ Ham's F-12 | Mirsky, R. and Jessen, K.: Isolation of Schwann Cell Precursors from Rodents., Methods Mol Biol 1739, 3-15, 2018 |
| 小鼠 | Mouse, embryonic | 皮层神经元 / Cortical neurons | PDS kit: per instructions | Neurobasal | Kramer, N., Haney, M., Morgens, D., Jovicic, A., Couthouis, J., Li, A., Ousey, J., Ma, R., Bieri, G., Tsui, C., Shi, Y., Hertz, N., Tessier-Lavigne, M., Ichida, J., Bassik, M. and Gitler, A.: CRISPR-Cas9 Screens in Human Cells and Primary Neurons Identify Modifiers of C9ORF72 Dipeptide-Repeat-Protein Toxicity., Nat Genet , , 2018 |
| 小鼠 | Mouse, P2-P12 | 背根神经节神经元 / Dorsal root ganglia neurons | Collagenase Type 2: 0.1% | MEM | Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G. and Demir, I.: 3D Cancer Migration Assay with Schwann Cells., Methods Mol Biol 1739, 317-325, 2018 |
| 小鼠 | Mouse, adult | 腰椎DRG / Lumbar DRG | Collagenase Type 2: 200 u/ml； Neutral Protease: 2.5 u/ml |
HBSS | Weng, Y., An, R., Cassin, J., Joseph, J., Mi, R., Wang, C., Zhong, C., Jin, S., Pfeifer, G., Bellacosa, A., Dong, X., Hoke, A., He, Z., Song, H. and Ming, G.: An Intrinsic Epigenetic Barrier for Functional Axon Regeneration, Neuron 94, 337, 2018 |
| 小鼠 | Mouse | 螺旋神经节 / Spiral ganglion | Collagenase Type 1: 0.1% | MEM | Meas, S., Nishimura, K., Scheibinger, M. and Dabdoub, A.: Front Neurosci 12, 822, 2018 |
| 小鼠 | Mouse, 4 month | 卫星胶质 / Satellite glial | Hyaluronidase: 0.2%； Collagenase Type 4: 0.2%； Collagenase: 0.2% |
HBSS | Tongtako, W., Lehmbecker, A., Wang, Y., Hahn, K., Baumgartner, W. and Gerhauser, I.: Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties., Sci Rep 7, 13915, 2017 |
| 小鼠 | Mouse | 神经球 / Neurospheres | Trypsin: 0.13%； Hyaluronidase: 0.08% |
Neurobasal A | Xu, W., Sachewsky, N., Azimi, A., Hung, M., Gappasov, A. and Morshead, C.: Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord., Stem Cells 35, 485-496, 2017 |
| 小鼠 | Mouse, day 2 | 雪旺 / Schwann | Trypsin: 0.025%； Collagenase Type 1: 0.1%； Deoxyribonuclease I: 7 u/ml； Collagenase Type 2: 0.2% |
DMEM | Clements, M., Byrne, E., Camarillo Guerrero, L., Cattin, A., Zakka, L., Ashraf, A., Burden, J., Khadayate, S., Lloyd, A., Marguerat, S. and Parrinello, S.: The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration., Neuron 96, 98-114.e7, 2017 |
| 小鼠 | Mouse, 2-3 month | 交感神经元 / Sympathetic neurons | Papain: 10 u/ml； Collagenase Type 2: 0.13%； Neutral Protease: 0.22% |
EBSS | Sun, H., Tsai, W., Li, B., Tao, W., Chen, P. and Rubart, M.: Voltage-Induced Calcium Release in Postganglionic Sympathetic Neurons in Adult Mice., PLoS ONE 11, e0148962, 2016 |
| 小鼠 | Mouse | 脊髓运动神经元，星形胶质细胞，小胶质细胞 / Spinal motor neurons, astrocytes, microglia | Papain: 0.2% | HBSS | Beaudet, M., Yang, Q., Cadau, S., Blais, M., Bellenfant, S., Gros-Louis, F. and Berthod, F.: High Yield Extraction of Pure Spinal Motor Neurons, Astrocytes and Microglia from Single Embryo and Adult Mouse Spinal Cord., Sci Rep 5, 16763, 2015 |
| 小鼠 | Mouse | 神经元 / Neurons | PDS kit: per instructions | EBSS | Ren, H., Lu, T., McGraw, T. and Accili, D.: Anorexia and Impaired Glucose Metabolism in Mice with Hypothalamic Ablation of Glut4 Neurons., Diabetes 64, 405-17, 2015 |
| 小鼠 | Mouse, adult | 星形胶质细胞 / Astrocytes | Papain: ~20 u/ml | HBSS | Choi, H., Sun, D. and Jakobs, T.: Isolation of Intact Astrocytes from the Optic Nerve Head of Adult Mice., Exp Eye Res 137, 103-10, 2015 |
| 小鼠 | Mouse | DRG 神经元 / DRG neurons | Collagenase Type 2: 0.1%； Trypsin: 0.25% |
DMEM/F-12 | Lee, B., Cho, H., Jung, J., Yang, Y., Yang, D. and Oh, U.: Anoctamin 1 Contributes to Inflammatory and Nerve-Injury Induced Hypersensitivity., Mol Pain 10, 5, 2014 |
| 小鼠 | Mouse, neonatal | 雪旺细胞 / Schwann cells | Collagenase: 0.05-0.1%； Trypsin: 0.125-0.25% |
DMEM | Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination., J Neuroinflammation 11, 63, 2014 |
| 小鼠 | Mouse | 少突胶质细胞 / Oligodendrocytes | Papain: 0.1%； Deoxyribonuclease I: 0.0002% |
HBSS | Harris, M., Hulseberg, P., Ling, C., Karman, J., Clarkson, B., Harding, J., Zhang, M., Sandor, A., Christensen, K., Nagy, A., Sandor, Matyas,S. and Fabry, Z.: Immune Privilege of the CNS is not the Consequence of Limited Antigen Sampling., Sci Rep 4, 4422, 2014 |
| 小鼠 | Mouse | 海马神经元 / Hippocampal neurons | PDS kit: per instructions | DMEM | Williams, E., Zhong, X., Mohamed, A., Li, R., Liu, Y., Dong, Q., Ananiev, G., Mok, J., Lin, B., Lu, J., Chiao, C., Cherney, R., Li, H., Zhang, S. and Chang, Q: Mutant Astrocytes Differentiated from Rett Syndrome Patients-Specific iPSCs Have Adverse Effects on Wild-Type Neurons., Hum Mol Genet 23, 2968-80, 2014 |
| 小鼠 | Mouse | 神经球 / Neurosperes | Papain: 0.1%； Deoxyribonuclease I: 0.1% |
NeuroCult NSC | Stuart, M., Corrigan, F. and Baune, B.: Knockout of CXCR5 Increases the Population of Immature Neural Cells and Decreases Proliferation in the Hippocampal Dentate Gyrus., J Neuroinflammation 11, 31, 2014 |
| 小鼠 | Mouse, 2 month | 成纤维细胞 / Fibroblasts | Papain: 0.2% | Hibernate | Liu, G., Rustom, N., Litteljohn, D., Bobyn, J., Rudyk, C., Anisman, H. and Hayley, S.: Use of Induced Pluripotent Stem Cell Derived Neurons Engineered to Express BDNF for Modulation of Stressor Related Pathology., Front Cell Neurosci 8, 316, 2014 |
| 小鼠 | Mouse, 4-6 week | 雪旺 / Schwann | Collagenase Type 1: 0.01%； Trypsin: 0.125% |
DMEM | Masaki, T., Qu, J., Cholewa-Waclaw, J., Burr, K., Raaum, R. and Rambukkana, A.: Reprogramming Adult Schwann Cells to Stem Cell-Like Cells by Leprosy Bacilli Promotes Dissemination of Infection., Cell 152, 51, 2013 |
| 小鼠 | Mouse | 少突胶质前体细胞 / Oligodendrocyte precursor cells | Papain: 0.09% | DMEM | Medina-Rodriguez, E., Arenzana, F., Bribian, A. and de Castro, F.: Protocol to Isolate a Large Amount of Functional Oligodendrocyte Precursor Cells from the Cerebral Cortex of Adult Mice and Humans., PLoS ONE 8, e81620, 2013 |
| 小鼠 | Mouse | 肠神经元 / Enteric neurons | Collagenase Type 4: 0.1%； Trypsin: 0.05%； Deoxyribonuclease I: 400 u/ml |
DMEM/F12 | Zhang, Y. and Hu, W.: Mouse Enteric Neuronal Cell Culture., Methods Mol Biol 1078, 55-63, 2013 |
| 小鼠 | Mouse, 6-8 week | 神经前体 / Neural precursors | Trypsin: 0.13%； Hyaluronidase: 0.08% |
see reference | Babona-Pilipos, R., Popovic, M. and Morshead, C.: A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field., J Vis Exp 68, e4193, 2012 |
| 小鼠 | Mouse, adult | 视网膜神经元 / Retinal neurons | Papain: 10-20 ul | HBSS | Goetz, J. and Trimarchi, J.: Single-Cell Profiling of Developing and Mature Retinal Neurons., J Vis Exp 62, e3824, 2012 |
| 小鼠 | Mouse | 神经元 / Neurons | Papain: 100 ul | DMEM | Li, Y., Roy, B., Wang, W., Zhang, L., Sampson, S. and Lin, D.: Imaging pHluorin-Tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons., J Vis Exp 69, e4450, 2012 |
| 小鼠 | Mouse, P4-5 | 神经鞘 / Neuroshere | Trypsin: 0.25%； Papain: 100 u； Deoxyribonuclease I: 0.025% |
Pro-N | Ziegler, A., Schneider, J., Qin, M., Tyler, W., Pintar, J., Fraidenraich, D., Wood, T and Levison, S.: IGF-II Promotes Stemness of Neural Restricted Precursors., Stem Cells 30, 1265, 2012 |
| 小鼠 | Mouse, 6-8 week | 神经干细胞 / Neural stem cells | Papain: 0.01%； Neutral Protease: 0.1%； Deoxyribonuclease I: 0.01% |
DMEM/F12 | Bracko, O., Singer, T., Aigner, S., Knobloch, M., Winner, B., Ray, J., Clemenson, G., Suh, H., Couillard-Despres, S., Aigner, L., Gage, F. and Jessberger, S.: Gene Expression Profiling of Neural Stem Cells and their Neuronal Progeny Reveals IGF2 as a Regulator of Adult Hippocampal Neurogenesis., J Neurosci 32, 3376-87, 2012 |
| 小鼠 | Mouse | 神经祖细胞 / Nerve progenitors | Collagenase Type 4: 0.025%； Trypsin NF 1:250: .025% |
HBSS | Salisbury, E., Lazard, Z., Ubogu, E., Davis, A. and Olmsted-Davis, E.: Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2., Stem Cells Transl Med 1, 874-85, 2012 |
| 小鼠 | Mouse, embryonic | 神经嵴 / Neural crest | Collagenase/Dispase: 0.1% | DMEM | Pfaltzgraff, E., Mundell, N. and Labosky, P.: Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube., J Vis Exp , e4134, 2012 |
| 小鼠 | Mouse, embryo, 14 day | 脊髓神经元 / Spinal cord neurons | Papain: 0.05%； Deoxyribonuclease I: 0.004% |
PBS/DMEM | Pollari, E., Savchenko, E., Jaronen, M., Kanninen, K., Malm, T., Wojciechowski, S., Ahtoniemi, T., Goldsteins, G., Giniatullina, R., Giniatullin, R., Koistinaho, J. and Magga, J.: Granulocyte Colony Stimulating Factor Attenuates Inflammation in a Mouse Model of Amyotrophic Lateral Sclerosis., J Neuroinflammation 8, 74, 2011 |
| 小鼠 | Mouse, 1-2 day | 少突胶质细胞，背根神经节 / Oligodendrocytes, dorsal root ganglia | Papain: 0.15%； Deoxyribonuclease I: 0.006% |
DMEM | O'Meara, R., Ryan, S., Colognato, H. and Kothary, R.: Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-Cultures from Post-Natal Murine Tissues., J Vis Exp 54, 3324, 2011 |
| 小鼠 | Mouse, embryonic | 运动神经元 / Motorneurons | Trypsin: 0.025% | HBSS | Conrad, R., Jablonka, S., Sczepan, T., Sendtner, M., Wiese, S. and Klausmeyer, A.: Lectin-Based Isolation and Culture of Mouse Embryonic Motoneurons., J Vis Exp 55, e3200, 2011 |
| 小鼠 | Mouse, 4-8 week | 嗅觉包裹 / Olfactory ensheathing | Collagenase: 0.15%； Papain: 12 u/ml |
DMEM | Radtke, C., Sasaki, M., Lankford, K., Gallo, V. and Kocsis, J.: CNPase Expression in Olfactory Ensheathing Cells., J Biomed Biotechnol 2011, 608496, 2011 |
| 小鼠 | Mouse | 齿状回神经前体 / Dentate gyrus neural precursor | Papain: 2.5 u/ml；
Neutral Protease: 1.0 u/ml； Deoxyribonuclease I: 250 u/ml |
Neurobasal A | Babu, H., Claasen, J., Kannan, S., Runker, A., Palmer, T. and Kempermann, G.: A Protocol for Isolation and Enriched Monolayer Cultivation of Neural Precursor Cells from Mouse Dentate Gyrus., Front Neurosci 5, 89, 2011 |
| 小鼠 | Mouse, 30 day | 运动神经元 / Motoneurons | Papain: 0.2% | Hibernate A | Milligan, C. and Gifondorwa, D.: Isolation and Culture of Postnatal Spinal Motoneurons., Methods Mol Biol 793, 77, 2011 |
| 小鼠 | Mouse | 海马和视网膜神经元 / Hippocampal and retinal neurons | Papain: 1%； Deoxyribonuclease I: 5 u/ml |
HBSS | Brown, J., Gianino, S. and Gutmann, D.: Defective cAMP Generation Underlies the Sensitivity of CNS Neurons to Neurofibromatosis-1 Heterozygosity, J Neurosci 30, 5579, 2010 |
| 小鼠 | Mouse | 中枢神经系统白细胞 / CNS leukocytes | Collagenase Type 4: 300 u/ml | HBSS | Sayed, B., Christy, A., Walker, M. and Brown, M.: Meningeal Mast Cells Affect Early T Cell Central Nervous System Infiltration and Blood-Brain Barrier Integrity Through TNF: a Role for Neutrophil Recruitment?, J Immunol 184, 6891-900, 2010 |
| 小鼠 | Mouse, adult | 脊髓小神经节 / Spinal microganglia | Papain: 0.2% | Hibernate A | Yip, P., Kaan, T., Fenesan, D. and Malcangio, M.: Rapid Isolation and Culture of Primary Microglia from Adult Mouse Spinal Cord., J Neurosci Methods 183, 223-37, 2009 |
| 小鼠 | Mouse, embryonic | 多巴胺能神经元 / Dopaminergic neurons | Trypsin: 0.1%； Deoxyribonuclease I: 0.02% |
DMEM | Radad Khaled, Gille Gabriele, Rausch Wolf-Dieter: Dopaminergic neurons are preferentially sensitive to long-term rotenone toxicity in primary cell culture, Toxicol In Vitro 22, 68-74, 2008 |
| 小鼠 | Mouse, neonatal | DRG 神经元 / DRG neurons | Collagenase: 0.2%； Trypsin: 0.05% |
Ham's F12 | Pedrola, L., Espert, A., Valdes-Sanchez, T., Sanchez-Piris, M., Sirkowski, E., Scherer, S., Farinas, I., and Palau, F.: Cell Expression of GDAP1 in the Nervous System and Pathogenesis of Charcot-Marie-Tooth Type 4A Disease., J Cell Mol Med 12, 679, 2008 |
| 小鼠 | Mouse, 6 mo | 神经元，神经球 / Neurons, neurospheres | Papain: 0.2% | Hibernate | Brewer Gregory J, Torricelli John R: Isolation and culture of adult neurons and neurospheres, Nat Protoc 2, 1490-8, 2007 |
| 小鼠 | Mouse | 神经的，各种 / Neural, various | Papain: 12 u/ml； Trypsin: see reference； Collagenase/Dispase: see reference |
see reference | Panchision David M, Chen Hui-Ling, Pistollato Francesca, Papini Daniela, Ni Hsiao-Tzu, Hawley Teresa S: Optimized flow cytometric analysis of central nervous system tissue reveals novel functional relationships among cells expressing CD133, CD15, and CD24, Stem Cells 25, 1560-70, 2007 |
| 小鼠 | Mouse, 6-8 week | 感觉神经元，DRG / Sensory neurons, DRG | Papain: 20 u/ml； Collagenase Type 2: 0.4%； Neutral Protease: 0.46% |
HBSS | Malin, S., Davis, B. and Molliver, D.: Production of Dissociated Sensory Neuron Cultures and Considerations for their use in Studying Neuronal Function and Plasticity, Nat Protoc 2, 152, 2007 |
| 小鼠 | Mouse, C57BL/6, 1 year | 神经元 / Neurons | Papain: 0.2% | DMEM | Eide, L, and McMurray, C: Culture of Adult Mouse Neurons, Biotechniques 38(1), 99-104, 2005 |
| 小鼠 | Mouse, adult | 脑和脊髓细胞 / Brain and spinal cord cells | Trypsin: 0.25% | PBS | Gonzalez John M, Bergmann Cornelia C, Fuss Babette, Hinton David R, Kangas Cindy, Macklin Wendy B, Stohlman Stephen A: Expression of a dominant negative IFN-gammareceptor on mouse oligodendrocytes, Glia 51, 22-34, 2005 |
| 小鼠 | Mouse, neonatal | 神经元 / Neurons | PDS kit: per instructions | EBSS | Gill JC, Moenter SM, Tsai PS: Developmental regulation of gonadotropin-releasing hormone neurons by fibroblast growth factor signaling, Endocrinology 145, 3830, 2004 |
| 小鼠 | Mouse, C57BL | 小脑颗粒细胞前体 / Cerebellar granule cell precursors | Papain: 16.5 u/ml； Deoxyribonuclease I: 0.008% |
Dulbecco's PBS | Okano-Uchida T, Himi T, Komiya Y, and Ishizaki Y: Cerebellar granule cell precursors can differentiate into astroglial cells, Proc Natl Acad Sci U S A 101, 1211, 2004 |
| 小鼠 | Mouse, 1-3 day | 神经元，神经节 / Neurons, ganglia | Papain: 20 u/ml；
Deoxyribonuclease I: 100 u/ml； Collagenase: 0.3%； Trypsin: 0.05%； |
HBSS | Savchenko V, Sung U, Blakely RD.: Cell surface trafficking of the antidepressant-sensitive norepinephrine transporter revealed with an ectodomain antibody, Mol Cell Neurosci 24, 1131, 2003 |
| 小鼠 | Mouse, male | 三叉感觉神经元 / Trigeminal senesory neurons | Papain: 20 u/ml | HEPES buffered saline | Roberts, L., MacDonald, C.and Mark, C.: Anandamide is a Partial Agonist at Native Vanilloid Receptors in Acutely Isolated Mouse Trigeminal Sensory Neurons., Br J Pharmacol 137, 421, 2002 |
| 小鼠 | Mouse, postnatal-day-1-old (P1) | 神经元 / Neurons | Trypsin: 0.25% | NGF-containing medium | Deshmukh, M., Kuida, K., and Johnson Jr., E.: Caspase Inhibition Extends the Commitment to Neuronal Death Beyond Cytochrome c Release to the Point of Mitochondrial Depolarization, J Cell Biol 150 (1), 131, 2000 |
| 小鼠 | Mouse embryos | 神经元，DRG 和 SCG / Neurons, DRG and SCG | Trypsin: 0.25% | L-15 medium | Lee, K., Davies, A., and Jaenisch, R.: P75-Eficient Embryonic Dorsal Root Sensory and Neonatal Sympathetic Neurons Display a Decreased Sensitivity to NGF, Development 120, 1027, 1994 |
| 小鼠 | mouse, 65 days | 神经元，神经元前体 / Neurons, neuronal precursors | Trypsin: 0.1%； Deoxyribonuclease I: 0.001% |
DMEM | Richards L., Kilpatrick T., and Bartlett P.: De Novo Generation of Neuronal Cells from the Adult Mouse Brain, Proc Natl Acad Sci U S A 89, 8591, 1992 |
| 小鼠 | Mouse, CD-1, neonate | 神经元，背根神经节 / Neurons, dorsal root ganglion | Trypsin: 0.25% | HBSS | Quinn, S. and De Boni, U.: Enhanced Neuronal Regeneration by Retinoic Acid of Murine Dorsal Root Ganglia and of Fetal Murine and human Spinal Cord in vitro, In Vitro Cell Dev Biol 27, 55, 1991 |
| 小鼠 | Mouse, fetal | 前体 / Precursor | Trypsin: 0.5% | PBS | Kitani, H., Shiurba, R., Sakakura, T., Tomooka, Y.: Isolation and Characterization Of Mouse Neural Precursor Cells in Primary Culture, In Vitro Cell Dev Biol 27, 615, 1991 |
| 小鼠 | Mouse (SWR or CF1), 1-3 months | 乳头，味觉感受器 / Papillae, taste receptor | Pronase E: 0.15% | Carbonate-Phosphate buffer | Spielman, A., Mody, I., Brand, J., Whitney, G., MacDonald, J., and Salter, M.: A Method for Isolating and Patch-Clamping Single Mammalian Taste Receptor Cells, Brain Res 503, 326, 1989 |
| 小鼠 | Mouse, neonatal | PNS 测试神经元 / PNS test neurons | Trypsin: 0.08% | Eagle's Basal Medium (see reference) | Varon, S., Skaper, S., Barbin, G., Selak, I., and Manthorpe, M.: Low Molecular Weight Agents Support Survival of Cultured Neurons From the Central Nervous System, J Neurosci 4 (3), 654, 1984 |
| 小鼠 | Mouse (BALB/c), adult | 神经元，脊髓 / Neurons, spinal cord | Collagenase Type 3: 0.25% | Hank's BSS, CMF | Eagleson, K. and Bennett, M.: Survival of Purified Motor Neurons In Vitro: Effects of Skeletal Muscle-Conditioned Medium, Neurosci Lett 38, 187, 1983 |
| 小鼠 | Mouse, 0-30 day | 神经 / Neural | Trypsin NF 1:250: 0.25% | BSS | Shrier, B., Wilson, S., and Nirenberg, M.: Cultured Cell Systems and Methods for Neurobiology, Vol. 32,, , 765, 1974 |
| 绵羊 | Lamb | 少突胶质细胞 / Oligodendroglia | Trypsin: 0.1% | (see reference) | Poduslo, S., Miller, K., and McKhann, G.: Metabolic Properties of Maintained Oligodendroglia Purified from Brain, J Biol Chem 253, 1592, 1978 |
| 猪 | Porcine, adult, 60-100 kg | 颈上神经节 / Superior cervical ganglia | Papain: 2 u/ml； Collagenase: 0.12%； Neutral Protease: 0.48% |
HBSS | Si ML, Lee TJ.: Presynaptic alpha7-nicotinic acetylcholine receptors mediate nicotine-induced nitric oxidergic neurogenic vasodilation in porcine basilar arteries, J Pharmacol Exp Ther 298, 122, 2001 |
| 鹌鹑 | Quail | 神经嵴 / Neural crest | Trypsin: 0.05% | MEM, HBSS | Sieber-Blum, M., and Cohen, A.: Clonal Analysis of Quail Neural Crest Cells: They are Pluripotent and Differentiate in Vitro in the Absence of Noncrest Cells, Dev Biol 80, 96, 1980 |
| 大鼠 | Rat, embryonic 14.5 day | 雪旺细胞前体 / Schwann cell precursors | Collagenase Type 2: 0.2%； Hyaluronidase: 0.12%； Soybean Trypsin Inhibitor: 0.03% |
DMEM/ Ham's F-12 | Mirsky, R. and Jessen, K.: Isolation of Schwann Cell Precursors from Rodents., Methods Mol Biol 1739, 3-15, 2018 |
| 大鼠 | Rat, SD, 3 month | 雪旺 / Schwann | Neutral Protease: 0.25%； Collagenase Type 1: 0.05% |
DMEM | Andersen, N. and Monje, P.: Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers., Methods Mol Biol 1739, 49-66, 2018 |
| 大鼠 | Rat, Wistar, 3 day | 背根神经节神经元 / Dorsal root ganglia neurons | Collagenase Type 2: 0.1% | MEM | Fangmann, L., Teller, S., Stupakov, P., Friess, H., Ceyhan, G. and Demir, I.: 3D Cancer Migration Assay with Schwann Cells., Methods Mol Biol 1739, 317-325, 2018 |
| 大鼠 | Rat, SD, day 2 | 雪旺 / Schwann | Collagenase Type 1: 0.1%； Trypsin: 0.25% |
DMEM | Maurel, P.: Preparation of Neonatal Rat Schwann Cells and Embryonic Dorsal Root Ganglia Neurons for In Vitro Myelination Studies., Methods Mol Biol 1739, 17-37, 2018 |
| 大鼠 | Rat, adult | 雪旺 / Schwann | Stemxyme 1: 0.05% | DMEM | George, D., Ahrens, P. and Lambert, S.: Satellite Glial Cells Represent a Population of Developmentally Arrested Schwann Cells., Glia , , 2018 |
| 大鼠 | Rat, 2-3 week | 背根神经节 / Dorsal root ganglia | Collagenase Type 1: 0.1% | HBSS | Lin, Y. and Chen, J.: Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release., J Vis Exp , , 2018 |
| 大鼠 | Rat, SD, p7 | 雪旺 / Schwann | Trypsin: 0.025%； Collagenase Type 1: 0.1%； Deoxyribonuclease I: 7 u/ml； Collagenase Type 2: 0.2% |
DMEM | Clements, M., Byrne, E., Camarillo Guerrero, L., Cattin, A., Zakka, L., Ashraf, A., Burden, J., Khadayate, S., Lloyd, A., Marguerat, S. and Parrinello, S.: The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration., Neuron 96, 98-114.e7, 2017 |
| 大鼠 | Rat, SD | 雪旺 / Schwann | Collagenase Type 1: 0.05% | DMEM | Schuh, C., Hercher, D., Stainer, M., Hopf, R., Teuschl, A., Schmidhammer, R. and Redl, H.: Extracorporeal Shockwave Treatment: A Novel Tool to Improve Schwann Cell Isolation and Culture., Cytotherapy 18, 760-70, 2016 |
| 大鼠 | Rat, E17 | 神经元 / Neurons | PDS kit: per instructions | DMEM | Hayakawa, K., Esposito, E., Wang, X., Terasaki, Y., Liu, Y., Xing, C., Ji. X. and Lo, E.: Transfer of Mitochondria from Astrocytes to Neurons after Stroke., Nature 535, 551-5, 2016 |
| 大鼠 | Rat, SD | 雪旺 / Schwann | Neutral Protease: 0.25%； Collagenase Type 1: 0.05%； Trypsin: 0.25% |
DMEM | Andersen, N., Srinivas, S., Pinero, G. and Monje, P.: A Rapid and Versatile Method for the Isolation, Purification and Cryogenic Storage of Schwann Cells from Adult Rodent Nerves., Sci Rep 6, 31781, 2016 |
| 大鼠 | Rat, embryonic and neonatal | 皮层神经元，DRG / Cortical neurons, DRG | PDS kit: per instructions； Collagenase Type 1: 0.17% |
Neurobasal | Rheaume, C., Cai, B., Wang, J., Fernandez-Salas, E., Aoki, K., Francis, J. and Broide, R.: A Highly Specific Monoclonal Antibody for Botulinum Neurotoxin Type A-Cleaved SNAP25., Toxins 7, 2354-70, 2015 |
| 大鼠 | Rat, SD, 6-9 week | 背根神经节 / Dorsal root ganglia | Collagenase Type 3: 0.2% | HBSS | Murayama, C., Watanabe, S., Nakamura, M. and, Norimoto, H.: Inhibitory Activity of Yokukansankachimpihange against Nerve Growth Factor-Induced Neurite Growth in Cultured Rat Dorsal Root Ganglion Neurons., Molecules 20, 14959-69, 2015 |
| 大鼠 | Rat | 背根神经节神经元 / Dorsal root ganglion neurons | Collagenase Type 2: 0.15% | HBSS | Yu, R., Seymour, V., Berecki, G., Jia, X., Akca, M., Adams, D., Kaas, Q. and Craik, D.: Less is More: Design of a Highly Stable Disulfide-Deleted Mutant of Analgesic Cyclic α-Conotoxin Vc1.1., Sci Rep 5, 13264, 2015 |
| 大鼠 | Rat, 6-8 week | 脊髓神经祖细胞 / Spinal cord neural progenitor | PDS kit: with modifications | EBSS | Mothe, A. and Tator, C.: Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord., J Vis Exp , e52732, 2015 |
| 大鼠 | Rat, SD | 三叉神经节 / Trigeminal ganglion | Collagenase Type 2: 0.1%； Papain: 20 u/ml |
DMEM/F12 | Wei, X., Yan, J., Tillu, D., Asiedu, M., Weinstein, N., Melemedjian, O., Price, T. and Dussor, G.: Meningeal Norepinephrine Produces Headache Behaviors in Rats via Actions Both on Dural Afferents and Fibroblasts., Cephalalgia 35, 1054-64, 2015 |
| 大鼠 | Rat, embryonic | 脑神经元 / Cerebral neurons | Papain: 2 u/ml； Deoxyribonuclease I: 0.01% |
DMEM-PBS | Oyanagi, K., Tashiro, T. and Negishi, T.: Cell-Type-Specific and Differentiation-Status-Dependent Variations in Cytotoxicity of Tributyltin in Cultured Rat Cerebral Neurons and Astrocytes., J Toxicol Sci 40, 459-68, 2015 |
| 大鼠 | Rat, neonatal | 雪旺细胞 / Schwann cells | Collagenase: 0.05-0.1%； Trypsin: 0.125-0.25% |
DMEM | Stettner, M., Lohmann, B., Wolffram, K., Weinberger, J., Dehmel, T., Hartung, H., Mausberg, A. and Kieseier, B.: Interleukin-17 Impedes Schwann Cell-Mediated Myelination., J Neuroinflammation 11, 63, 2014 |
| 大鼠 | Rat, 1-3 day | 海马神经元 / Hippocampal neurons | Papain: 25 u/ml | L-15 | Thurner, P., Gsandtner, I., Kudlacek, O., Choquet, D., Nanoff, C., Freissmuth, M. and Zezula, J.: A Two-State Model for the Diffusion of the A2A Adenosine Receptor in Hippocampal Neurons: Agonist-Induced Switch to Slow Mobility is Modified by Synapse-Associated Protein 102 (SAP102)., J Biol Chem 289, 9263-74, 2014 |
| 大鼠 | Rat, SD, embryonic | 下丘脑神经元 / Hypothalamic neuronal | PDS kit: per instructions | Neurobasal A | Loktev, A. and Jackson, P.: Neuropeptide Y Family Receptors Traffic via the Bardet-Biedl Syndrome Pathway to Signal in Neuronal Primary Cilia., Cell Rep 5, 1316-29, 2013 |
| 大鼠 | Rat, E18 | 海马神经元 / Hippocampal neurons | Papain: 0.2% | Hibernate | Todd, G., Boosalis, C., Burzycki, A., Steinman, M., Hester, L., Shuster, P. and Patterson, R.: Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density Hippocampal Neurons., PLoS ONE 8, e58996, 2013 |
| 大鼠 | Rat, SD, 175-200 g | 三叉神经节 / Trigeminal ganglia | Papain: 20 u/ml； Collagenase Type 2: 0.3% |
HBSS | Yan, J., Melemedjian, O., Price, T. and Dussor, G.: Sensitization of Dural Afferents Underlies Migraine-Related Behavior Following Meningeal Application of Interleukin-6 (IL-6)., Mol Pain 8, 6, 2012 |
| 大鼠 | Rat, E19-21 and P1-3 | 交感神经元 / Sympathetic neurons | Collagenase Type 2: 0.1%； Neutral Protease: 0.5% |
DMEM/F-12 | Ghogha, A., Bruun, D. and Lein, P.: Inducing Dendritic Growth in Cultured Sympathetic Neurons., J Vis Exp 61, e3546, 2012 |
| 大鼠 | Rat, 20-30 day | 背根神经节 / Dorsal root ganglion | Papain: 20 u/ml； Collagenase Type 1: 0.3%； Neutral Protease: 0.4% |
L-15 | Bosmans, F., Puopolo, M., Martin-Eauclaire, M., Bean, B., Swartz, K.: Functional Properties and Toxin Pharmacology of a Dorsal Root Ganglion Sodium Channel Viewed through its Voltage Sensors., J Gen Physiol 138, 59, 2011 |
| 大鼠 | Rat, Wistar, E18 | 神经元 / Neurons | Papain: 0.05% | Neurobasal | Giacomello, M., Girardi, S., Scorzeto, M., Peruffo, A., Maschietto, M., Cozzi, B. and Vassanelli, S.: Stimulation of Ca²+ Signals in Neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors., J Neurosci Methods 198, 1, 2011 |
| 大鼠 | Rat, SD, 200-300 g | 背根神经节，成纤维细胞 / Dorsal root ganglia, fibroblast | Collagenase: 0.125% | DMEM | East, E., de Oliveira, D., Golding, J. and Phillips, J.: Alignment of Astrocytes Increases Neuronal Growth in Three-Dimensional Collagen Gels and is Maintained Following Plastic Compression to Form a Spinal Cord Repair Conduit., Tissue Eng Part A 16, 3173, 2010 |
| 大鼠 | Rat, Fisher, 7-21 month | 海马神经元 / Hippocampal neurons | Papain: 0.2% | Hibernate A | Chen, N., Newcomb, J., Garbuzova-Davis, S., Davis Sanberg, C., Sanberg, P. and Willing, A.: Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro., Aging Dis 1, 173, 2010 |
| 大鼠 | Rat, SD, E18 | 神经元 / Neuronal | Papain: 0.2% | Neurobasal E | Peltier, D., Simms, A., Farmer, J and Miller, D.: Human Neuronal Cells Possess Functional Cytoplasmic and TLR-Mediated Innate Immune Pathways Influenced by Phosphatidylinositol-3-Kinase Signaling, J Immunol 184, 7010, 2010 |
| 大鼠 | Rat, SD, 7 day | 小脑颗粒神经元 / Cerebellar granule neurons | PDS kit: per instructions | PBS | Tanaka, S., Shaikh, I., Chiocca, E. and Saeki, Y.: The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development., PLoS ONE 4, e5922, 2009 |
| 大鼠 | Rat, SD, embryonic day 18 | 海马神经元 / Hippocampal neurons | Papain: 20 u/ml | Neurobasal/B27 | Liu, Y., Yohrling, G., Wang, Y., Hutchinson, T., Brenneman, D., Flores, C., Zhao, B.: Carisbamate, a Novel Neuromodulator, Inhibits Voltage-Gated Sodium Channels and Action Potential Firing of Rat Hippocampal Neurons., Epilepsy Res 83, 66, 2009 |
| 大鼠 | Rat, embryonic | 下丘脑神经元和胶质细胞 / Hypothalamic neurons and glias | Papain: 0.5 u/ml | DMEM | Yokosuka Makoto, Ohtani-Kaneko Ritsuko, Yamashita Kayoko, Muraoka Daisuke, Kuroda Yoichiro, Watanabe Chiho: Estrogen and environmental estrogenic chemicals exert developmental effects on rat hypothalamic neurons and glias, Toxicol In Vitro 22, 1-9, 2008 |
| 大鼠 | Rat, Wistar, 7 day | 颈上神经节 / Superior cervical ganglion | Collagenase: 0.05% | L-15 | Sakisaka, T., Yamamoto, Y., Mochida, S., Nakamura, M., Nishikawa, K., Ishizaki, H., Okamoto-Tanaka, M., Miyoshi, J., Fujiyoshi, Y., Manabe, T. and Takai, Y.: Dual Inhibition of SNARE Complex Formation by Tomosyn Ensures Controlled Neurotransmitter Release., J Cell Biol 183, 323, 2008 |
| 大鼠 | Rat, neonatal | 多巴胺神经元 / Dopamine neurons | Papain: 20 u/ml | PBS | Frank, L., Caldera-Siu, A. and Pothos, E.: Primary Dissociated Midbrain Dopamine Cell Cultures from Rodent Neonates., J Vis Exp 21, 820, 2008 |
| 大鼠 | Rat, Wistar, 4 day | 海马神经元 / Hippocampal neurons | PDS kit: per instructions | Neurobasal A | Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo: Cross-talk of vitamin D and glucocorticoids in hippocampal cells, J Neurochem 96, 500-9, 2006 |
| 大鼠 | Rat, SD, neonatal | 星形胶质细胞 / astrocytes | PDS kit: per instructions | DMEM | Lacroix-Fralish, M., Tawfik, V., Nutile-McMenemy, N., Harris, B. and Deleo, J.: Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons., Neuron Glia Biol 2, 227, 2006 |
| 大鼠 | Rat, 2 month | 脊髓祖细胞 / Spinal cord progenitor cells | PDS kit: see reference | Neurobasal A | Mothe, A., Kulbatski, I., Van Bendegem, R., Lee, L., Kobayashi, E., Keating, A., and Tator, C.: Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells, J Histochem Cytochem 53(10), 1215, 2005 |
| 大鼠 | Rat, SD, E19 | 背根神经节 / Dorsal root ganglia | PDS kit: per instructions | MEM/Ham's F12 | Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.: AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties, J Pharmacol Exp Ther 313, 474, 2005 |
| 大鼠 | Rat, Wistar, E15 | 犁鼻受体神经元 / Vomeronasal receptor neurons | Collagenase/Dispase: 0.1%； Papain: 0.5 u/ml |
DMEM/F12 | Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M: Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons, Chem Senses 30, 111, 2005 |
| 大鼠 | Rat, SD, 1-2 day | 皮质星形胶质细胞 / Cortical astrocytes | Papain: see reference | DMEM | Floyd Candace L, Gorin Fredric A, Lyeth Bruce G: Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes, Glia 51, 35-46, 2005 |
| 大鼠 | Rat, SD, 0-2 day | 颈上神经节 / Superior cervical ganglion | Collagenase Type 4: 20 u/ml； Trypsin: 0.25% |
DMEM | Pedraza Carlos E, Podlesniy Petar, Vidal Noemi, Arevalo Juan Carlos, Lee Ramee, Hempstead Barbara, Ferrer Isidre, Iglesias Montse, Espinet Carme: Pro-NGF isolated from the human brain affected by Alzheimer's disease induces neuronal apoptosis mediated by p75NTR, Am J Pathol 166, 533-43, 2005 |
| 大鼠 | Rat, SD, male, 5-8 week | 三叉神经元 / Trigeminal neurons | Papain: 20 u/ml； Collagenase: 0.3% |
CMF Hanks | Connor, M, Naves, L. and McCleskey, E.: Contrasting Phenotypes of Putative Proprioceptive and Nociceptive Trigeminal Neurons Innervating Jaw Muscle in Rat., Mol Pain 1, 31, 2005 |
| 大鼠 | Rat, fetal | 脑干细胞和皮层神经元 / Brainstem and cortical neurons | PDS kit: per instructions | DMEM | Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ: Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells, Endocrinology 145, 3495, 2004 |
| 大鼠 | Rat, SD, male 150-220 g | 背根神经节神经元 / Dorsal root ganglion neurons | Collagenase Type 4: 0.125%； Trypsin: 0.05% |
DMEM/Ham's F12 | Hu Hong-Zhen, Gu Qihai, Wang Chunbo, Colton Craig K, Tang Jisen, Kinoshita-Kawada Mariko, Lee Lu-Yuan, Wood Jackie D, Zhu Michael X: 2-aminoethoxydiphenyl borate is a common activator of TRPV1, TRPV2, and TRPV3, J Biol Chem 279, 35741-8, 2004 |
| 大鼠 | Rat, 15 week | 神经元 / Neurons | Papain: 0.2% | Hibernate A | Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH, and Shaw G: Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem, J Neurophysiol 87, 1076, 2002 |
| 大鼠 | Rat, SD, male, 270-330 g | 脊髓祖细胞 / Spinal progenitor cells | PDS kit: with modifications | Neurobasal medium | Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.: Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain, Cell Transplant 11, 17, 2002 |
| 大鼠 | Rat, Fisher, 8-9 week | 成年祖细胞 / Adult progenitor | Papain: 2.5 u/ml； Deoxyribonuclease I: 250 u/ml； Neutral Protease: 1 u/ml |
DMEM/F-12 | Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.: The adult substantia nigra contains progenitor cells with neurogenic potential, J Neurosci 22, 6639, 2002 |
| 大鼠 | Rat, SD, embryonic | 坐骨神经和肠神经嵴干细胞 / Sciatic nerve and gut neural crest stem | Collagenase Type 4: 0.025%； Trypsin: 0.005%； Deoxyribonuclease I: 0.05% |
HBSS | Bixby, S., Kruger, G., Mosher, J., Joseph, N. and Morrison, S.: Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity., Neuron 35, 643, 2002 |
| 大鼠 | Rat | 神经元，海马 / Neurons, hippocampal | Papain: 15 - 20 u/ml | Eagle's MEM | Liu, Q., Kawai, H., and Berg, D.: B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons, PNAS 98 (8), 4734, 2001 |
| 大鼠 | Rat, newborn, 7 days old | 神经元 / Neurons | Trypsin: 0.25% | MEM10 | Acosta, C., Fabrega, A., Masco, D., and Lopez, H.: A Sensory Neuron Subpopulation with Unique Sequential Survival Dependence on Nerve Growth Factor and Basic Fibroblast Growth Factor during Development, J Neurosci 21 (22), 8873, 2001 |
| 大鼠 | Rat, embryonic 18-19 day | 海马神经元 / Hippocampal neurons | PDS kit: see reference | DMEM | Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M: Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo, J Neurosci 21, 9204-13, 2001 |
| 大鼠 | Rat, embryonic, day 18 | 皮质 / Cortical | Papain: | Neurobasal medium and DMEM | O'Connor, S., Andreadis, J., Shaffer, K., Ma, W., Pancrazio, J., and Stenger, D.: Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications, Biosensors & Bioelectronics 14, 871, 2000 |
| 大鼠 | Rat, Wister, postnatal, P1-3 days | 神经元，海马 / Neurons, hippocampal | Papain: 20 u/ml | EBSS | Neuhoff, H., Roeper, J., Schweizer, M.: Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons, Eur J Neurosci 11, 4241, 1999 |
| 大鼠 | Rat, SD, female, pregnant | 坐骨神经 / Sciatic nerves | Trypsin: 0.025%； Collagenase Type 3: 0.1% |
L-15 medium | Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999 |
| 大鼠 | Rat | 脊髓 / Spinal cord | Trypsin: 0.133% | HBSS and PIPES | Johansson, C., Momma, S., Clarke, D., Risling, M., Lendahl, U., and Frisen, J: Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System, Cell 96, 25, 1999 |
| 大鼠 | Rat, SD, embryonic | 坐骨神经 / Sciatic nerves | Trypsin: 0.025%； Collagenase Type 3: 0.1% |
L-15 medium | Morrison, S., White, P., Zock, C., and Anderson, D.: Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells, Cell 96, 737, 1999 |
| 大鼠 | Rat, (Long Evans), 2-5 day old | 神经元，海马 / Neurons, hippocampal | Papain: 20 u/ml | EBSS | Wilding, T., and Huettner, J.: Activation and Desensitization of Hippocampal Kainate Receptors, J Neurosci 17 (8), 2713, 1997 |
| 大鼠 | Rat, Wister and SD, newborn, 0-21 days, either sex | 肌间神经节 / Myenteric ganglia | Trypsin: 0.05% | MEM-HEPES | Schafer, K., Saffrey, M., Burnstock, G., and Mastres-Ventura, P.: A New Method for the Isolation of Myenteric Plexus from the Newborn Rat Gastrointestinal Tract, Brain Res Proto 1, 109, 1997 |
| 大鼠 | Rat, pups, 24-48 h old | 神经元，海马 / Neurons, hippocampal | Papain: 20 u/ml | Harvest buffer | Hall, R., and Soderling, T.: Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons, J Biol Chem 272 (7), 4135, 1997 |
| 大鼠 | Rat, SD, adult, 250-300g, P8 | 神经元，DRG / Neurons, DRG | Neutral Protease: 0.5% | L-15 w/ CO2 | Davies, S., Fitch, M., Memberg, S., Hall, A, Raisman, G., and Silver, J.: Regeneration of Adult Axons in White Matter Tracts of the Central Nervous System, Nature 390, 680, 1997 |
| 大鼠 | Rat, 1-4 day old | 神经元，海马 / Neurons, hippocampal | Papain: 20 u/ml | MEM | Twitchell, W., Brown, S., and Mackie, K.: Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons, J Neurophysiol 78, 43, 1997 |
| 大鼠 | Rat, SD, Fisher | 海马神经元 / Hippocampal neurons | Papain: 0.2% | HibernateA/B27 | Brewer, G.J.: Isolation and culture of adult rat hippocampal neurons of any age, J Neurosci Methods 71, 143, 1997 |
| 大鼠 | Rat, E18 | 海马神经元 / Hippocampal neurons | Papain: 20 u/ml； Deoxyribonuclease I: 0.01% |
MEM | Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL: Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons, J Neurosci 16, 2912-23, 1996 |
| 大鼠 | Rat, 1-6 day | DRG 神经元 / DRG neurons | Papain: 20 u/ml； Collagenase Type 1: 150 u/ml； Neutral Protease: 0.8% |
HBSS | Robertson, S., Rae, M., Rowan, E. and Kennedy, C.: Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia., Br J Pharmacol 118, 951, 1996 |
| 大鼠 | Rat, pups, 14 day old | 神经元，交感神经 / Neurons, sympathetic | Neutral Protease: 0.24% | HBSS | McFarlane, S., and Cooper, E.: Extrinsic Factors Influence the Expression of Voltage-Gated K Currents on Neonatal Rat Sympathetic Neurons, J Neurosci 13 (6), 2591, 1993 |
| 大鼠 | Rats, SD, 11-14 day old, 29-32 g | 基底前脑神经元 / Basal forebrain neurons | Trypsin: 0.125% | Gey's BSS | Allen, T., Sim, J., and Brown, D.: The Whole-Cell Calcium Current in Acutely Dissociated Magnocellular Cholinergic Basal Forebrain Neurones of the Rat, J Physiol 460, 91, 1993 |
| 大鼠 | Rat, Wistar-Hanover, 7-12 day | 产后多巴胺神经元 / Postnatal dopamine neurons | Trypsin: 0.035% | (see reference) | Rayport, S., Sulzer, D., Shi, W., Sawasdikosol, S., Monaco, J., Batson, D., and Rajendran, G.: Identified Postnatal Mesolimbic Dopamine Neurons in Culture: Morphology and Electrophysiology, J Neurosci 12 (11), 4264, 1992 |
| 大鼠 | Rat, SD, 18-day-old, fetus | 海马 / Hippocampal | Trypsin: 0.2% | HBSS | Cheng, B., and Mattson, M.: IGF-I and IGF-II Protect Cultured Hippocampal and Septal Neurons Against Calcium-Mediated Hypoglycemic Damage, J Neurosci 12 (4), 1558, 1992 |
| 大鼠 | Rat | 干细胞，神经嵴 / Stem, neural crest | Collagenase: 0.075% | Ringer's solution | Stemple, D., and Anderson, D.: Isolation of a Stem Cell for Neurons and Glia from the Mammalian Neural Crest, Cell 71, 973, 1992 |
| 大鼠 | Rat, SD, pregnant, Charles River | 锥体神经元，非锥体神经元 / Pyramidal neurons，Nonpyramidal neurons | Trypsin: 0.027% | HEPES | Buchhalter, J., and Dichter, M.: Electrophysiological Comparison of Pyramidal and Stellate Nonpyramidal Neurons in Dissociated Cell Culture of Rat Hippocampus, Brain Res Bull 26, 333, 1991 |
| 大鼠 | Rat, SD, embryos, 19-21 days gestation | 雪旺，背根神经节 / Schwann, dorsal root ganglia | Trypsin: 0.25% | HBSS, CMF | Mithen, F., Reiker, M., and Birchem, R.: Effects of Ethanol on Rat Schwann Cell Proliferation and Myelination in Culture, In Vitro Cell Dev Biol 26, 129, 1990 |
| 大鼠 | Rat | 心脏，肾上腺嗜铬，副神经元 / Heart，Adrenal chromaffin，Paraneurons | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| 大鼠 | Rat, SD, 8 day old pups | 小脑神经元 / Cerebellar neurons | Trypsin: 0.025% | Eagle's MEM | Novelli, A., Reilly, J., Lysko, P., and Henneberry, R.: Glutamate Becomes Neurotoxic Via the N-methyl-D-aspartate Receptor When Intracellular Energy Levels are Reduced, Brain Res 451, 205, 1988 |
| 大鼠 | Rat, fetuses, 18 day old | 海马神经元 / Hippocampal neurons | Trypsin: 0.2% | Eagle's MEM | Mattson, M., and Kater, S.: Isolated Hippocampal Neurons in Cryopreserved Long-Term Cultures: Development of Neuroarchitecture and Sensitivity to Nmda, Int J Dev Neurosci 6 (5), 439, 1988 |
| 大鼠 | Rat, postnatal | 间隔神经元 / Septal neurons | Papain: 0.05% | PBS, CMF | Hatanaka,H., Tsukui, H., Nihonmatsu, I.: Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor, Neurosci Lett 79, 85, 1987 |
| 大鼠 | Rat, Long Evans, 1-15 days | 神经元，视觉皮层 / Neurons, visual cortex | Papain: 20 u/ml | BSS | Huettner, J., and Baughman, R.: Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats, J Neurosci 6, 3044, 1986 |
| 大鼠 | Rat, SD, female, 4 month old | 中枢神经系统细胞 / CNS cells | Trypsin: 0.25% | EBSS | Wood, P., and Bunge, R.: Evidence That Axons are Mitogenic for Oligodendrocytes Isolated From Adult Animals, Nature 320, 756, 1986 |
| 大鼠 | Rat, embryo, 15 day | 背角神经元，脊髓 / Dorsal horn neurons，Spinal | Trypsin: 0.025% | Ham's F-12 | Jahr, C. and Jessell, T.: Synaptic Transmission between Dorsal Root Ganglion and Dorsal Horn Neurons in Culture: Antagonism of Monosynaptic Excitatory Postsynaptic Potentials and Glutamate Excitation by Kynurenate, J Neurosci 5, 2281, 1985 |
| 大鼠 | Rat, fetus, 18-20 day | 海马神经元 / Hippocampal neurons | Trypsin: 0.25% | HBSS, CMF | Bartlett, W. and Banker, G.: An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts, J Neurosci 4, 1944, 1984 |
| 大鼠 | Rat, postnatal | 神经节，视网膜 / Ganglion, retina | Papain: 12.5 u/ml | HBSS w/5 mM HEPES | Leifer, D., Lipton, S., Barnstable, C., and Masland, R.: Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture, Science 224, 303, 1984 |
| 大鼠 | Rat, SD, pups | 视网膜 / Retina | Trypsin: 0.25% | Ham's F-12 | Sarthy PV, Curtis BM, and Catterall WA.: Retrograde Labeling, Enrichment, and Characerization of Retinal Ganglion Cells from the Neonatal Rat, J Neurosci 3 (12), 2532, 1983 |
| 大鼠 | Rat, W/FU, 5-8 day | 神经元和神经胶质 / Neurons and glial | Trypsin: 0.25% | MEM | Raff, M., Fields, K., Hakomori, S., Mirsky, R., Pruss, R., and Winter, J.: Cell-Type-Specific Markers for Distinguishing and Studying Neurons And the Major Classes of Glial Cells in Culture, Brain Res 174, 283, 1979 |
| 大鼠 | Rat, fetus | 神经元，交感神经 / Neurons, sympathetic | Trypsin: 0.25% | L-15 or HBSS, CMF | Wakshull, E., Johnson, M., Burton, H.: Postnatal Rat Sympathetic Neurons In Culture. 1. A Comparison With Embryonic Neurons, J Neurophysiol 42, 1410, 1979 |
| 大鼠 | Rat, Wistar/Furth, newborn | 雪旺 / Schwann | Trypsin: 0.25% | DMEM | Brockes, J., Fields, K., and Raff, M.: Studies on Cultured Rat Schwann Cells. I. Establishment of Purified Populations From Cultures of Peripheral Nerve, Brain Res 165, 105, 1979 |
| 大鼠 | Rat, embryo | 神经元，皮质 / Neurons, cortical | Trypsin: 0.027% | MEM | Dichter, M.: Rat Cortical Neurons in Cell Culture: Culture Methods, Cell Morphology, Electrophysiology, and Synapse Formation, Brain Res 149, 279, 1978 |
| 大鼠 | Rat, newborn | 神经元，交感神经 / Neurons, sympathetic | Collagenase Type 1: 0.01% | Hank's solution, CF | Reichardt, L., Patterson, P.: Neurotransmitter Synthesis and Uptake by Isolated Sympathetic Neurones in Microcultures, Nature 270, 147, 1977 |
| 大鼠 | Rat, neonatal | 神经元，颈上神经节 / Neurons, superior cervical ganglia | Trypsin: 0.1% | Basal L-15 medium | Mains, R., and Patterson, P.: Primary Cultures of Dissociated Sympathetic Neurons I. Establishment of Long-Term Growth in Culture and Studies of Differentiated Properties, J Cell Biol 59, 329, 1973 |
| 蝾螈 | Salamander, 18-25 cm | 视网膜 / Retina | Papain: 14 u/ml | Saline | Townes-Anderson, E., MacLeish, P., and Raviola, E.: Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase, J Cell Biol 100, 175, 1985 |
| 蝾螈 | Salamander (A.tigrinum) | 光感受器细胞，视网膜 / Photoreceptors, retina | Papain: 0.05% | (see reference) | Bader, C., MacLeish, P., and Schwartz, E.: Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina, Proc Natl Acad Sci U S A 75, 3507, 1978 |
| 贝类 | Aplysia californica | 神经元 / Neurons | Neutral Protease: 1.0% | L-15-ASW | Lee, A., Decourt, B. and Suter, D.: Neuronal Cell Cultures from Aplysia for High-Resolution Imaging of Growth Cones., J Vis Exp 12, 662, 2008 |
| 贝类 | Snails (Helisoma trivolis), albino, adult | 颊神经节， SLT 肌肉 / Buccal ganglia， SLT muscle | Trypsin: 0.2% | DMEM | Zoran, M., Doyle, R. and Haydon, P.: Target Contact Regulates the Calcium Responsiveness of the Secretory Machinery During Synaptogenesis, Neuron 6, 145, 1991 |
| 贝类 | Pond snail (Helisoma) albino, adult | Somata，颊神经节 / Somata, buccal ganglia | Trypsin: 0.2% | Antibiotic saline, Leibowitz 50% | Haydon, P.: The Formation of Chemical Synapses Between Cell-Cultured Neuronal Somata, J Neurosci 8, 1032, 1988 |
| 贝类 | Helisoma trivovlis | 颊神经节 / Buccal ganglia | Trypsin: 0.2% | L-15 medium | Cohan, C., Haydon, P., and Kater, S.: Single Channel Activity Differs in Growing and Nongrowing Growth Cones of Isolated Identified Neurons of Helisoma, J Neurosci Res 13, 285, 1985 |
| 贝类 | Mollusc, juvenile 1-2 gm or adult 50-100 gm | 神经元，LUQ细胞 / Neurons，LUQ cells | Protease: 1% | L15 medium | Schacher, S., and Proshanksy, E.: Neurite Regeneration by Aplysia Neurons in Dissociated Cell Culture: Modulation by Aplysia Hemolymph and the Presence of the Initial Axonal Segment, J Neurosci 3 (12), 2403, 1983 |
| 贝类 | Aplysia californica | LUQ细胞，RUQ细胞 / LUQ cells，RUQ cells | Protease: 1% | L15 medium | Camardo, J., Proshansky, E., and Schacher, S.: Identified Aplysia Neurons Form Specific Chemical Synapses in Culture, J Neurosci 3 (12), 2614, 1983 |
| 龟 | Turtle (Pseudemys scripta elegans) | 视网膜 / Retinal | Papain: 0.1% (13.5 u/mg) | Kreb's Ringer | Lam, D.: Biosynthesis of Acetylcholine in Turtle Photoreceptors, Proc Natl Acad Sci U S A 69, 1987, 1972 |
