Worthington 淋巴结组织分离指南

原代细胞分离中,针对淋巴结组织涉及到树突状,基质细胞、直肠腺、肺引流和滤泡树突 等等的解离通常会使用酶,如何选择合适的酶来完成对应淋巴结组织的解离,以及原代细胞采集的常规操作, 艾美捷针对淋巴结相关的组织分类解离分种属、不同的细胞、酶组合推荐,推荐浓度、培养基以及参考文献,详细汇总指南:

Worthington 组织分离指南

本技术指南描述了标准实验室程序; 为建立细胞分离方案提供了一种合乎逻辑的实验方法; 并列出了许多组织特异性参考资料,以帮助开发有效的方法。更多其他组织的解离指南,点击查看:各组织分离指南

淋巴结
种属 种属详情 细胞名称 酶组合推荐,含推荐浓度 培养基 参考文献
小鼠 Mouse 树突状 / Dendritic Collagenase Type 4: 0.1%;
Deoxyribonuclease I: 0.1%
RPMI 1640 Terhorst, D., Chelbi, R., Wohn, C., Malosse, C., Tamoutounour, S., Jorquera, A., Bajenoff, M., Dalod, M., Malissen, B. and Henri, S.: Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis., J Immunol 195, 4953-61, 2015
小鼠 Mouse 基质细胞 / Stromal Collagenase Type 4: 0.1%;
Deoxyribonuclease I: 0.004%
RPMI Dubrot, J., Duraes, F., Potin, L., Capotosti, F., Brighouse, D., Suter, T., LeibundGut-Landmann, S., Garbi, N., Reith, W., Swartz, M. and Hugues, S.: Lymph Node Stromal Cells Acquire Peptide-MHCII Complexes from Dendritic Cells and Induce Antigen-Specific CD4+T Cell Tolerance, J Exp Med 211, 1153, 2014
小鼠 Mouse 肺引流 / Lung draining Collagenase Type 4: 0.1-0.125% HBSS Rayamajhi, M., Redente, E., Condon, T., Gonzalez-Juarrero, M., Riches, D. and Lenz, L.: Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry., J Vis Exp 51, 2702, 2011
小鼠 Mouse 滤泡树突 / Follicular dentritic Collagenase Type 4: 0.25%;
Deoxyribonuclease I: 0.5%
HBSS Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, Szakal AK: Follicular dendritic cell (FDC) precursors in primary lymphoid tissues, J Immunol 160, 1078-84, 1998
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