原代细胞分离中，针对上皮细胞组织涉及到上皮，气管、直肠腺、结肠上皮和胃肠上皮、鼻上皮 等等的解离通常会使用酶，如何选择合适的酶来完成对应上皮细胞组织的解离，以及原代细胞采集的常规操作， 艾美捷针对上皮细胞相关的组织分类解离分种属、不同的细胞、酶组合推荐，推荐浓度、培养基以及参考文献，详细汇总指南：
本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
| 上皮细胞 | |||||
| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 牛 | Bovine, fetal | 上皮，气管 / Epithelial，Tracheal | Neutral Protease: 2% | Dissociation medium, CMF | Schumann, B.L., Cody, T.E., Miller, M.L., Leikauf, G.D.: Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells, In Vitro Cell Dev Biol 24, 211, 1988 |
| 犬/狗 | Dog | 气管 / Tracheal | Pronase: 0.1% | DMEM | Virmani, A., Naziruddin, B., Desai, V., Lowry, J., Graves, D., and Sachdev, G.: Evidence for Secretion of High Molecular Weight Mucins by Canine Tracheal Epithelial Cells in Primary Culture: Effects of Select Secretagogues on Mucin Secretion, In Vitro Cell Dev Biol 28A, 120, 1992 |
| 鸡 | Chick, 5 day old | 肠间充质和上皮 / Intestinal mesenchymal and epithelial | Collagenase: 0.03% | DMEM | Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995 |
| 鱼 | Shark (Squalus acanthias) | 直肠腺 / Rectal gland | Collagenase: 0.2% | Ringer's solution | Karnaky, Jr., K.J., Valentich, J.D., Currie, M.G., Oehlenschlager, W.F., and Kennedy, M.P.: Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells, Am J Physiol 260, 1125, 1991 |
| 鱼 | Shark (Squalus acanthias) | 直肠腺 / Rectal gland | Collagenase: 0.2% | Ringer's solution | Valentich, J: Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport, J Tiss Cul Meth 13, 149, 1991 |
| 鱼 | Winter flounder, 200-500 g | 肾小管 / Renal tubule | Trypsin: 0.2% | CMF solution | Dickman, K. and Renfro, J.: Primary Culture of Flounder Renal Tubule Cells:Transepithelial Transport, Am J Physiol 251, 424, 1986 |
| 青蛙 | Frog, Xenopus laevis, adult, female | 结肠上皮 / Colonic epithelial | Collagenase Type 4: 0.1% | Kreb's | Heinke, B, and Clauss, W.: Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium, J Comp Physiol [B] 169, 148, 1999 |
| 豚鼠 | Guinea-pig, Hartley, female, 200 g | 上皮 / Epithelial | Collagenase Type 1: 0.1% | DMEM | Rutten, M.: Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells, J Tiss Cul Meth 14, 235, 1992 |
| 豚鼠 | Guinea-pig, Hartley albino, | 子宫内膜 / Endometrial | Collagenase: 0.25% | HBSS | Chaminadas, G., Alkhalaf, M., Remy-Martin, J.P., Propper, A.Y., and Adessi, G.L.: Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium, J Endocrinol 123, 233, 1989 |
| 仓鼠 | Hamster, Syrian gold, male, 100-120 g | 气管 / Tracheal | Pronase: 0.1% | MEM with Hepes, CMF | Niles,R., Kim, K.C., Hyman, B., Christensen, T., Wasano, K., Brody, J.: Characterization Of Extended Primary And Secondary Cultures Of Hamster Tracheal Epithelial Cells, In Vitro Cell Dev Biol 24, 457, 1988 |
| 仓鼠 | Hamster, Syrian golden (strain CR:RGH) | 气管 / Tracheal | Trypsin: 0.1% | Ham's F-12 | McDowell, E., et al.: Differentiation of Tracheal Mucociliary Epithelium in Primary Cell Culture Recapitulates Normal Fetal Development and Regeneration Following Injury in Hamsters, Am J Pathol 129, 511, 1987 |
| 仓鼠 | Hamster (strain 1516 EHS and Lakeview), 8-12 weeks | 气管 / Tracheal | Trypsin: 0.05% | Medium 199 | Lee, T., Wu, R., Brody, A., Barrett, J., and Nettesheim, P.: Growth and Differentiation of Hamster Tracheal Epithelial Cells in Culture, Exp Lung Res 6, 27, 1984 |
| 仓鼠 | Hamster, Syrian gold, male, 6 weeks- 4 months | 气管 / Tracheal | Trypsin: 0.25% | PBS with EDTA | Goldman, W.E., Baseman, J.B.: Selective Isolation and Culture of a Proliferating Epithelial Cell Population from the Hamster Trachea, In Vitro 16, 313, 1980 |
| 人 | Human | 上皮 / Epithelial | Collagenase Type 4: 0.1%； Neutral Protease: 0.1% |
PBS | Gottipamula, S., Saraswat, S. and Sridhar, K.: Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells., Cytotherapy 19, 263-271, 2017 |
| 人 | Human | 口腔黏膜上皮 / Oral mucosal epithelial | Collagenase Type 1: 0.05% | DMEM/F12 | Hsueh, Y., Huang, S., Lai, J., Ma, S., Chen, H., Wu, S., Wang, T., Sun, C., Ma, K., Chen, J., Lai, C. and Ma, D.: Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation., Sci Rep 6, 36266, 2016 |
| 人 | Human | 胃肠上皮 / Gastrointestinal epithelial | Collagenase Type 1: 0.2% | DMEM/F12 | VanDussen, K., Marinshaw, J., Shaikh, N., Miyoshi, H., Moon, C., Tarr, P., Ciorba, M. and Stappenbeck, T.: Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays., Gut 64, 911-20, 2015 |
| 人 | Human | 人气管上皮 / Human tracheal epithelium | Protease Type XIV: 0.04% | DMEM/F12 | Widdicombe JH, Sachs LA, Morrow JL, and Finkbeiner WE.: Expansion of cultures of human tracheal epithelium with maintenance of differentiated structure and function, Biotechniques 39(2), 249, 2005 |
| 人 | Human | 结肠上皮 / Colonic epithelial | Collagenase:； Neutral Protease: 0.3%； Deoxyribonuclease I: 0.05% |
RPMI 1640 | Fukushima, K. and Fiocchi, C.: Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients., Am J Physiol Gastrointest Liver Physiol 286, G804-13, 2004 |
| 人 | Human | 角膜缘上皮片 / Corneal limbal epithelial sheet | Neutral Protease: 5% | see reference | Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R, and Tseng SC: Novel enzymatic isolation of an entire viable human limbal epithelial sheet, Inv Ophthal Visual Sci 44(10), 4275, 2003 |
| 人 | Human | 肠上皮 / Intestinal epithelial | Collagenase Type 4: 72.5 u/ml | HBSS | Fahlgren, A., Hammarstrom, S., Danielsson, A. and Hammarstrom, M.: Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis., Clin Exp Immunol 131, 90, 2003 |
| 人 | Human | 胃上皮细胞 / Gastric epithelial cells | Collagenase Type 2: 200 u/ml； Neutral Protease: 1.2 u/ml； Soybean Trypsin Inhibitor: 0.125% |
L-15 | Smoot, D., Sewchand, J., Young, K., Desbrodes, B., Allen, C. and Naab, T.: A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells, Meth Cell Sci 22, 133, 2000 |
| 人 | Human | 胃 / Gastric | Collagenase Type 4: 0.01% | F-12 medium | Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R and Little, C: The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:10.1, 1995 |
| 人 | Human | 鼻息肉上皮 / Nasal polyp epithelial | Neutral Protease: .004%； Trypsin: 0.1% |
see reference | Halbert CL, Alexander IE, Wolgamot GM, Miller AD: Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells, J Virol 69, 1473-9, 1995 |
| 人 | Human | 上皮 / Epithelial | Trypsin: 0.05% | DMEM | Sabatini, L., Allen-Hoffmann, B, Warner, T., and Azen, E.: Serial Cultivation of Epithelial Cells from Human and Macaque Salivary Glands, In Vitro Cell Dev Biol 27A, 939, 1991 |
| 人 | Human | 上皮 / Epithelial | Trypsin: 0.2% | MEM, PBS | Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991 |
| 人 | Human | 上皮 / Epithelial | Collagenase: 2.0% | DMEM/Ham's F-12 | Emerman, J. and Wilkinson, D.: Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples, In Vitro Cell Dev Biol 26, 1186, 1990 |
| 人 | Human | 上皮 / Epithelial | Pronase: 0.1% | PBS | Gruenert, D.C., Basbaum, C.B., and Widdicombe, J.H.: Long-Term Culture of Normal and Cystic Fibrosis Epithelial Cells Grown Under Serum-Free Conditions, In Vitro Cell Dev Biol 26, 411, 1990 |
| 人 | Human | 上皮 / Epithelial | Collagenase Type 2: 0.2% | (see reference) | Wood, L. and Neufeld, E.: A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux, J Biol Chem 265, 12796, 1990 |
| 人 | Human | 子宫内膜上皮 / Endometrial epithelial | Trypsin: | DMEM/Ham's F-12 | Munson, L., Chandler, S., and Schlafer, D.: Cultivation of Bovine Fetal and Adult Endometrial Epithelial Cells, J Tiss Cul Meth 11 (3), 129, 1988 |
| 人 | Human | 上皮 / Epithelial | Deoxyribonuclease I: 0.01% | HEPES with 5.9mM Glucose,5mM DTT | Widdicombe, J.H., Coleman, D.L., Finkbeiner, W.E., and Tuet, I.K.: Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa, J Appl Physiol 58, 1729, 1985 |
| 人 | Human | 上皮 / Epithelial | Protease Type XIV: 0.1% | Eagle's MEM | Yankaskas, J., Cotton, C., Knowles, M., Gatzy, J., and Boucher, R.: Culture of Human Nasal Epithelial Cells on Collagen Matrix Supports, Am Rev Respir Dis 132, 1281, 1985 |
| 人 | Human, women, 27-49 years | 上皮 / Epithelial | Trypsin: 0.125% | HBSS, CMF | Auersperg, N., Siemens, C.H., and Myrdal, S.E.: Human Ovarian Surface Epithelium In Primary Culture, In Vitro 20, 743, 1984 |
| 人 | Human, infant and neonate | 上皮 / Epithelial | Trypsin: 0.1% | HBSS | Lechner, J., Babcock, M., Marnell, M., Narayan, K., and Kaighn, M.: Normal Human Prostate Epithelial Cell Cultures, Methods Cell Biol 21, 195, 1980 |
| 小鼠 | Mouse, 8-12 week | 鼻上皮 / Nasal epithelium | Papain: 20 u/ml； Deoxyribonuclease I: 0.005% |
EBSS | Brann, D., Tsukahara, T., Weinreb, C., Lipovsek, M., Van den Berge, K., Gong, B., Chance, R., Macaulay, I., Chou, H., Fletcher, R., Das, D., Street, K., de Bezieux, H., Choi, Y., Risso, D. and Dudoit, S.: Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia., Sci. Adv. 6, , 2020 |
| 小鼠 | Mouse | 肺泡上皮 / Alveolar epithelial | Neutral Protease: 0.1%； Deoxyribonuclease I: 0.01% |
DMEM | Sun, F., Xiao, G. and Qu, Z.: Isolation of Murine Alveolar Type II Epithelial Cells., Bio-protocol 7, , 2017 |
| 小鼠 | Mouse | 气管上皮 / Tracheal epithelial | Papain: 50 u/ml | See reference | Pardo-Saganta, A., Tata, P., Law, B., Saez, B., Chow, R., Prabhu, M., Gridley, T. and Rajagopal, J.: Parent Stem Cells Can Serve as Niches for Their Daughter Cells., Nature 523, 597-601, 2015 |
| 小鼠 | Mouse, adult | 纤毛上皮 / Cilary epithelial | PDS kit: per instructions | EBSS | Gualdoni, S., Baron, M., Lakowski, J., Decembrini, S., Pearson, R., Ali, R. and Sowden, J.: Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells., Curr Protoc Stem Cell Biol Chapter 1, Unit 1H.4, 2011 |
| 小鼠 | Mouse, male, 8-16 week | 肾小管上皮 / Renal tubular epithelial | Collagenase: 200 u/ml； Soybean Trypsin Inhibitor: see reference |
HBSS | Breggia, A. and Himmelfarb, J.: Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress., Oxid Med Cell Longev 1, 33, 2008 |
| 小鼠 | Mouse | 上皮 / Epithelial | Collagenase: 0.025%； Neutral Protease: 0.25% |
DMEM | Mathew, R., Degenhard,t K., Haramaty, L., Karp, C. and White, E.: Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer., Methods Enzymol 446, 77-106, 2008 |
| 小鼠 | Mouse, 6-8 week | 固有层单核细胞 / Lamina propria mononuclear cells | Collagenase Type 2: 0.015%； Deoxyribonuclease I: 0.01% |
RPMI | Wirtz S., Becker C., Blumberg R., Galle P., and Neurath M.: Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA, J Immunol 168(1), 411, 2002 |
| 小鼠 | Mouse, 11 week | 上皮 / Epithelial | Collagenase Type 3: 25 u/ml； Hyaluronidase: 0.1%； Protease XIV: 0.05%； Deoxyribonuclease I: 0.04% |
DMEM/F12 | Mueller, S., Clark, J., Myers, P. and Korach, K.: Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha., Endocrinology 143, 2357, 2002 |
| 小鼠 | Mouse, female | 唾液腺上皮 / Salivary gland epithelial | Collagenase Type 1: 750 u/ml； Hyaluronidase: 500 u/ml |
DMEM/F12 | Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y: Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis, Am J Pathol 155, 173-81, 1999 |
| 小鼠 | Mouse, fetal 12-13 day | 肠间充质和上皮 / Intestinal mesenchymal and epithelial | Collagenase: 0.03% | DMEM | Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995 |
| 小鼠 | Mouse | 颌下唾液 / Submandibular salivary | Collagenase Type 2 or 3: 0.16% | DMEM | Durban, E: Submandibular Salivary Epithelial Cells,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12B:2.1, 1995 |
| 小鼠 | Mouse | 食道 / Esophageal | Trypsin: 0.25% | PBS, CMF | Katayama, M., Kan, M.: Heparin-Binding (Fibroblast) Growth Factors re Potential Autocrine Regulators of Esophageal Epithelial Cell Proliferation, In Vitro Cell Dev Biol 27, 533, 1991 |
| 小鼠 | Mouse, mature, female, 6-8-wk-old | 子宫 / Uterine | Trypsin: 0.2% | HBSS | Ghosh, D., Danielson, K., Alston, J., and Heyner, S.: Functional Differentiation of Mouse Uterine Epithelial Cells Grown on Collagen Gels or Reconstituted Basement Membranes, In Vitro Cell Dev Biol 27A, 713, 1991 |
| 小鼠 | Mouse, female, 18 - 20 days | 子宫 / Uterine | Trypsin: 0.25% | HBSS | Fukamachi, H., and McLachlan, J.: Proliferation and Differentiation of Mouse Uterine Epithelial Cells in Primary Serum-Free Culture: Estradiol-17B Suppresses Uterine Epithelial Proliferation Cultured on a Basement Membrane-Like Substratum, In Vitro Cell Dev Biol 27A, 907, 1991 |
| 小鼠 | Mouse, (BALB/c), male, 3-5 months | 上皮 / Epithelial | Collagenase Type 3: 0.16% , 1:1 v/v | DMEM with 15 mM HEPES | Durban, E.M.: Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix, In Vitro Cell Dev Biol 26, 33, 1990 |
| 小鼠 | Mouse, female | 上皮 / Epithelial | Pepsin: 0.1% and 0.05% | HBSS with 0.2% EDTA, CMF | Riser, M., Huff, B., and Medina, D.: Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells, In Vitro 19, 730, 1983 |
| 小鼠 | Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant | 上皮 / Epithelial | Collagenase Type 3: 0.1% | HBSS | Yang, J., Flynn, D., Larson, L., and Hamamoto, S.: Growth in Primary Culture of Mouse Submandibular Epithelial Cells, In Vitro 18, 435, 1982 |
| 小鼠 | Mouse (BALB/cfC3H) | 乳腺肿瘤 / Mammary tumors | Collagenase: 1.0% | HBSS | Yang, J., Guzman, R., Richards, J., and Nandi, S.: Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels, In Vitro 16, 502, 1980 |
| 小鼠 | Mouse, C3H, 6-8 weeks | 上皮 / Epithelial | Collagenase: 0.10% | DMEM | Lillehaug, J., Mondal, S., and Heidelberger, C.: Establishment of Epithelial Cell Lines from Mouse Regenerating Liver, In Vitro 15, 910, 1979 |
| 猪 | Porcine, 5-60 kg | 视网膜色素上皮细胞 / Retinal pigment epithelial cells | Collagenase: 2% | DMEM | Wiencke, A., Kiilgaard, J., Nicolini, J., Bundgaard, M., Ropke, C., and La Cour, M.: Growth of Cultured Porcine Retinal Pigment Epithelial Cells, Acta Opthalmol Scand 81(2), 170, 2003 |
| 猪 | Porcine | 气管 / Trachea | Neutral Protease: 0.2% | HBSS | De Buysscher, E., Kennedy, J., and Mendicino, J.: Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis, In Vitro 20, 433, 1984 |
| 兔 | Rabbit, New Zealand white, adult | 结肠 / Colon | Neutral Protease: 0.3% | PBS | Vidrich, A., Racindranath, R., Farsi, K., and Targan, S.: A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures, In Vitro Cell Dev Biol 24 (3), 188, 1988 |
| 兔 | Rabbit, New Zealand white, male, 4-5lb. | 胃 / Gastric | Collagenase Type 2: 0.08% | Sodium phosphate buffer | Chew, C.S., Brown,M.R.: Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa, Biochim Biophys Acta 888, 116, 1986 |
| 兔 | Rabbit, New Zealand white estrous, female, 4-5 months | 间皮和表面上皮 / Mesothelial and surface epithelial | Trypsin: 0.125%-0.5% | Medium 199 | Nicosia, S., Johnson, J., and Streibel, E.: Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium), Int J Gynecol Pathol 3, 348, 1984 |
| 兔 | Rabbit, fetal | 上皮 / Epithelial | Collagenase Type 3: 0.10% | HBSS | Logsdon, C.D., Bisbee, C.A., Rutten, M.J. and Machen, T.E.: Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel, In Vitro 18, 233, 1981 |
| 大鼠 | Rat | 乳腺上皮 / Mammary epithelial | Collagenase Type 3: 0.35% | HBSS | Mei, N., McDaniel, L., Dobrovolsky, V., Guo, X., Shaddock, J., Mittelstaedt, R., Azuma, M., Shelton, S., McGarrity, L., Doerge, D. and Heflich, R.: The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats., Toxicol Sci 115, 412, 2010 |
| 大鼠 | Rat, SD, adult, male, 8-10 weeks old | 曲细精管 / Seminiferous tubules | Trypsin: 0.05% | Krebs-Ringer bicarbonate buffer | Abou-Haila, A., and Tulsiani, D.: Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization, Biol Proced Online 3 (1), 35, 2001 |
| 大鼠 | Rat, male | 肺泡上皮 / Alveolar epithelial | Elastase: 40 u/ml | DMEM | Planus, E., Galiacy, S., Matthay, M., Laurent, V., Gavrilovic, J., Murphy, G., Clerici, C., Isabey, D., Lafuma, C., and d'Ortho, M.: Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair, J Cell Sci 112, 243, 1999 |
| 大鼠 | Rat, embryonic | 气管上皮 / Tracheal epithelial | Collagenase Type 4: 0.05%； Neutral Protease:； Deoxyribonuclease I: |
DMEM/F12 | Shannon JM, Gebb SA, and Nielsen LD: Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture, Development 126, 1675, 1999 |
| 大鼠 | Rat, fetal, 14-15 day | 肠间充质和上皮 / Intestinal mesenchymal and epithelial | Collagenase: 0.03% | DMEM | Simon-Assmann, P and Kedinger, M: Embryonic Gut-Dissagregated Culture,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 12A:3.1, 1995 |
| 大鼠 | Rat, 4-14 week | 视网膜色素上皮细胞 / Retinal pigment epithelial cells | Collagenase Type 1: 65 u/ml； Hyaluronidase: 220 u/ml |
CF Hanks with EDTA | Wang N, Koutz CA, and Anderson RE.: A method for the isolation of retinal pigment epithelial cells from adult rats, Inv Ophthal Visual Sci 34(1), 101, 1993 |
| 大鼠 | Rat, SD, adult, 90-120 day old, 350-450 g | 附睾上皮 / Epididymal epithelial | Collagenase Type 2: 0.1% | HBSS | Klinefelter, G.: A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats, J Tiss Cul Meth 14, 195, 1992 |
| 大鼠 | Rat, 6 day old | 大鼠肠上皮 / Rat intestinal epithelial | Neutral Protease: 0.01%； Collagenase: 300 u/ml |
DMEM | Evans GS, Flint N, Somers AS, Eyden B, and Potten CS.: The development of a method for the preparation of rat intestinal epithelial cell primary cultures, J Cell Sci 101, 219, 1992 |
| 大鼠 | Rat, SD, male, 150 - 250 g | 上皮，胃 / Epithelial，Stomach | Pronase: 0.15% | Medium 199 | Dial, E., Kao, Y., and Lichtenberger, L.: Effects of 16,16-Dimethyl Prostaglandin E2 On Glycoprotein And Lipid Synthesis of Gastric Epithelial Cells Grown in a Primary Culture, In Vitro Cell Dev Biol 27, 39, 1991 |
| 大鼠 | Rat, adult | 小叶间导管碎片 / Interlobular duct fragments | Papain: | DMEM/Ham's F-12 | Heimann, T., and Githens, S.: Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix, Pancreas 6 (5), 514, 1991 |
| 大鼠 | Rat, SD, 200 g | 结肠 / Colon | Deoxyribonuclease I: 10 µg/ml | (see reference) | Yassin, R., Clearfield, H., Katz, S., and Murthy, S.: Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro, Peptides 12, 63, 1991 |
| 大鼠 | Rat, Wistar, neonatal | 上皮 / Epithelial | Trypsin: 0.1% | HBSS | Jassal, D., Han, R., Caniggia, I., Post, M., and Tanswell, A.: Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium, In Vitro Cell Dev Biol 27A, 625, 1991 |
| 大鼠 | Rodent, various (see reference) | 上皮 / Epithelial | Trypsin: 0.2% | MEM, PBS | Robinson, C., and Wu, R.: Culture of Conducting Airway Epithelial Cells in Serum-Free Medium, J Tiss Cul Meth 13, 95, 1991 |
| 大鼠 | Rat, 6-8 day | 视网膜色素上皮 / Retinal pigment epithelial | Neutral Protease: 2% | DMEM | Chang CW, Roque RS, Defoe DM, and Caldwell RB.: An improved method for isolation and culture of pigment epithelial cells from rat retina, Curr Eye Res 10(11), 1081, 1991 |
| 大鼠 | Rat (ACI/NMs X BUF/Mna) F1, male, 28 months | 上皮 / Epthelial | Collagenase Type 3: 0.1% | Eagle's MEM Serum-free | Masuda, A., Ohtsuka, K., and Matsuyama, M.: Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus, In Vitro Cell Dev Biol 26, 713, 1990 |
| 大鼠 | Rat, Fisher 344, male, 8 wks old, | 气管上皮 / Tracheal epithelial | Pronase: 0.5% | DMEM | Chang, L., Wu, R., and Nettesheim, P.: Morphological Changes in Rat Tracheal Cells During The Adaptive and Early Growth Phase in Primary Cell Culture, J Cell Sci 74, 283, 1985 |
| 大鼠 | Rat, Fischer, male, 4-6 weeks | 上皮 / Epithelial | Hyaluronidase: 0.1% | HEPES BSS | Babcock, M., Marino, M., Gunning, W., and Stoner, G.: Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells, In Vitro 19, 403, 1983 |
| 大鼠 | Rat, Fischer, Lewis and SD, male, 10-18 months | 上皮 / Epithelial | Trypsin: 0.05% | HBSS CMF | Herring, A., Raychaudhuri, R., Kelley, S., and Iybe, P.: Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats, In Vitro 19, 576, 1983 |
| 大鼠 | Rat, SD, female | 上皮，癌症和肿瘤 / Epithelial, cancer and tumor | Collagenase: 0.1% | Eagles's MEM | Cohen, L.: Isolation and Characterization of a Serially Cultivated, Neoplastic, Epithelial Cell Line from the N-nitrosomethylurea Induced Rat Mammary Adenocarcinoma, In Vitro 18, 565, 1982 |
| 大鼠 | Rat, Wistar, 12 day | 上皮 / Epithelial | Trypsin: 0.05% | HBSS, CMF | Malan-Shibley, L., and Iype, P.: Influence of Cultures on Cell Morphology/Tyrosine Aminotransferase Levels, Exp Cell Res 131, 363, 1981 |
| 大鼠 | Rat, Fischer, adult, 200-250 g | 上皮 / Epithelial | Hyaluronidase: 0.0075% | KCl-NaCl HEPES Buffer | Williams, G., and Gunn, J.: Long-Term Culture of Adult Rat Liver Epithelial Cells, Exp Cell Res 89, 139, 1974 |
| 大鼠 | Rat, Fischer, 10 day | 上皮样 / Epithelial-like | Trypsin: 0.25% | PBS | Williams, G., Weisburger, E., and Weisburger, J.: Isolation and Long-Term Cell Culture of Epithelial-Like Cells from Rat Liver, Exp Cell Res 69, 106, 1971 |
