组织分离/原代细胞分离，是酶在组织培养研究和细胞生物学研究中的主要应用。尽管多年来在这些应用中广泛使用酶，但它们在解离和收获中的作用机制尚不清楚。
研究人员在科学文献中搜索有关理想酶和组织解离的最佳条件的信息时，经常会遇到相互矛盾的数据。大部分变化源于细胞外基质的复杂性和动态性，以及在细胞分离应用中使用相对粗糙、不确定的酶制剂。
本指南总结了Worthington如何使用这些酶来完成对应肾上腺组织的解离，以及原代细胞采集的常规操作。 本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
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| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.1-0.2%； Deoxyribonuclease I: 0.003-0.015%； Hyaluronidase: 0.015% |
DMEM/F12 | Dominguez, N., Rodri茆uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012 |
| 牛 | Bovine, 6 month | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.125% | Locke's solution | Moustafa T, Girod S, Tortosa F, Li R, Sol JC, Rodriguez F, Bastide R, Lazorthes Y, Sallerin B: Viability and functionality of bovine chromaffin cells encapsulated into alginate-PLL microcapsules with a liquefied inner core, Cell Transplant 15, 121-33, 2006 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin cells | Collagenase: 0.1%； Deoxyribonuclease I: 30 u/ml |
DMEM | Hahm, S., Chen, Y., Vinson, C. and Eiden, L.: A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization., Mol Pharmacol 64, 1503, 2003 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.2% | Locke's solution | Ortega, J., Sagen, J., and Pappas, G.: Short-term Immunosuppression Enhances Long-term Survival of Bovine Chromaffin Cell Xenografts in Rat CNS, Cell Transplant 1, 33, 1992 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Deoxyribonuclease I: 30 u/mg | HEPES | Zhu, J., Li, W., Toews, M., and Hexum, T.: Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process, J Pharmacol Exp Ther 263 (3), 1479, 1992 |
| 牛 | Bovine (also rat) | 心脏，肾上腺嗜铬，副神经元 / Heart，Adrenal chromaffin，Paraneurons | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.25 % | DMEM | Dahmer, M., Hart, P., and Perlman, R.: Studies on the Effect of Insulin-Like Growth Factor-I on Catecholamine Secretion from Chromaffin Cells, J Neurochem 54 (3), 931, 1990 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.2%； Deoxyribonuclease I: 0.015% |
Locke's solution | Higgins, L., and Berg, D.: Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells, J Neurosci 7 (6), 1792, 1987 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.05% | Locke's solution, CMF | Aunis, D., Rotllan, P., and Miras-Portugal, M.: Incorporation of Adenosine into Nucleotides of Chromaffin Cells in Culture, Neurochem Int 7, 89, 1985 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.15% | Kreb's, CMF | Almazan, G., Aunis, D., Garcia, A., Montiel, C., Nicolas, G., and Sanchez-Garcia, P.: Effects Of CLS on the Release of Noradrenaline From Chromaffin Cells, Br J Biomed Sci 81, 599, 1984 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.1% | (see reference) | Pollard, H., Pazoles, C., Creutz, C., Scott, J., Zinder, O., and Hotchkiss, A.: An Osmotic Mechanism For Exocytosis From Dissociated Chromaffin Cells, J Biol Chem 259, 1114, 1984 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.05% | CF Kreb's | Cena, V., Garcia, A., Montiel, C., and Sanchez-Garcia, P.: Uptake of [3H]-nicotine and [3H]-noradrenaline by Cultured Chromaffin Cells, Br J Pharmacol 81, 119, 1984 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.025% | HBSS, modified | Waymire, J., Bennett, W., Boehme, R., Hankins, L., Gilmer-Waymire, K., and Haycock, J.: Bovine Adrenal Chromaffin Cells: High-Yield Purification and Viability in Suspension Culture, J Neurosci Methods 7, 329, 1983 |
| 牛 | Bovine | 髓质 / Medulla | Hyaluronidase: 0.2% | Saline w/BSA 0.5% | Knight, D. and Baker, P.: Stimulus-Secretion Coupling in Isolated Bovine Adrenal Medullary Cells, Q J Exp Physiol 68, 123, 1983 |
| 牛 | Bovine | 髓质 / Medulla | Collagenase: 0.2% | Krebs-Ringer bicarbonate buffer, CMF | Greenberg, A. and Zinder, O.: alpha- and beta-Receptor Control of Catecholamine Secretion from Isolated Adrenal Medulla Cells, Cell Tissue Res 226, 655, 1982 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Deoxyribonuclease I: 15 痢/ml | Medium A (see reference) | Wilson, S.P., and Viveros, O.H.: Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium, Exp Cell Res 133, 159, 1981 |
| 牛 | Bovine | 髓质 / Medulla | Protease: 0.2% | Saline | Baker, P., and Knight., D: Calcium Control of Exocytosis and Endocytosis in Bovine Adrenal Medullary Cells, Phil Trans R Soc Lond 296, 83, 1981 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.05% | Locke's solution, CMF | Trifaro, J.M., and Lee, R.W.: Morphological Characteristics and Stimulus-Secretion Coupling in Bovine Adrenal Chromaffin Cell Cultures, Neuroscience 5, 1533, 1980 |
| 牛 | Bovine, adult (also rat, Hanover-Wistar, young; guinea-pig, newborn) | 嗜铬细胞 / Chromaffin | Collagenase: 0.5% | HBSS | Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.25% | F-12 medium | Kumakura, K., Karoum, F., Guidotti, A., and Costa, E.: Modulation of Nicotinic Receptors by Opiate Receptor Agonists in Cultured Adrenal Chromaffin Cells, Nature 283, 489, 1980 |
| 牛 | Bovine | 髓质 / Medulla | Collagenase: 0.05% | Locke's solution, CF | Kilpatrick, D., Ledbetter, F., Carson, K., Kirshner, A., Slepetis, R., and Kirshner, N.: Stability of Bovine Adrenal Medulla Cells in Culture, J Neurochem 35 (3), 679, 1980 |
| 牛 | Bovine, adult | 髓质 / Medulla | Collagenase Type 1: 0.5% | HBSS | Unsicker, K., and Griesser, G.: Establishment, Characterization and Fibre Outgrowth of Isolated Bovine Adrenal Medullary Cells in Long-Term Cultures, Neuroscience 5, 1445, 1980 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Collagenase: 0.05% | DMEM | Aunis, D., Guerold, B., Bader, M-F., and Cieselski-Treska, J.: Immunocytochemical and Biochemical Demonstration of Contractile Proteins in Chromaffin Cells, Neuroscience 5, 2261, 1980 |
| 牛 | Bovine | 髓质 / Medullary | Collagenase Type 2: 0.2% | HEPES | Hersey, R., and DiStefano, V.: Control of Phenylethanolamine N-Methyltransferase by Glucocorticoids in Cultured Bovine Adrenal Medullary Cells, J Pharmacol Exp Ther 209 (1), 147, 1979 |
| 牛 | Bovine | 包皮 / Foreskin | Collagenase: 0.5% | Dulbecco's MEM w/10% calf serum | Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979 |
| 牛 | Bovine | 髓质 / Medulla | Collagenase Type 1: 0.05% | Kreb's, CF | Fenwick, E., Fajdiga, P., Howe, N., and Livett, B.: Functional and Morphological Characterization of Isolated Bovine Adrenal Medullary Cell, J Cell Biol 76, 12, 1978 |
| 牛 | Bovine | 嗜铬细胞 / Chromaffin | Hyaluronidase: 0.2% | HEPES, CF | Brooks, J.C.: The Isolated Bovine Adrenomedullary Chromaffin Cell: A Model of Neuronal Excitation-Secretion, Endocrinology 101, 1369, 1977 |
| 牛 | Bovine, male | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.2% | Kreb's Ringer bicarbonate buffer, CF | Schneider, A., Herz, R., and Rosenheck, K.: Stimulus-Secretion Coupling in Chromaffin Isolated From Adrenal Medulla, Proc Natl Acad Sci U S A 74, 5036, 1977 |
| 豚鼠 | Guinea-pig, 500-700 g | 嗜铬，髓质 / Chromaffin，Medulla | Collagenase: | BSS (see reference) | Role, L.W., Leeman, S.E., and Perlman, R.L.: Somatostain and Substance P Inhibit Catecholamine Secretion from Isolated Cells of Guinea-pig Adrenal Medulla, Neurochem Int 6, 1813, 1981 |
| 豚鼠 | Guinea-pig (also rat, Hanover-Wistar, young; newborn; cattle) | 嗜铬细胞 / Chromaffin | Collagenase: 0.5% | HBSS | Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980 |
| 豚鼠 | Guinea-pig | 肾上腺，嗜铬 / Adrenal，Chromaffin | Collagenase: 0.05%-0.20% | Kreb's-Ringer bicarb glucose buffer, CF | Hochman, J., and Perlman, R.L.: Catecholamine Secretion by Isolated Adrenal Cells, Biochim Biophys Acta 421, 168, 1976 |
| 仓鼠 | Hamster (Mesocricetus auratus) 100-150 g | 肾上腺，嗜铬 / Adrenal，Chromaffin | Hyaluronidase: 0.20% | Kreb's Ringer bicarbonate buffer | Liang, B.T., and Perlman, R.L.: Catecholamine Secretion by Hamster Adrenal Cells, J Neurochem 32, 927, 1979 |
| 人 | Human | 肾上腺髓质祖细胞 / Adrenal medulla progenitor | Collagenase: 0.2% | DMEM/F-12 | Santana, M., Chung, K., Vukicevic, V., Rosmaninho-Salgado, J., Kanczkowski, W., Cortez, V., Hackmann, K., Bastos, C., Mota, A., Schrock, E., Bornstein, S., Cavadas, C. and Ehrhart-Bornstein, M.: Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla., Stem Cells Transl Med 1, 783, 2012 |
| 人 | Human | 嗜铬细胞 / Chromaffin cells | Collagenase: 0.2% | Locke's solution | Jeon, Y., Baek, W., Chung, S., Shin, N., Kim, H., and Lee, S.: Cultured Human Chromaffin Cells Grafted in Spinal Subarachnoid Space Relieves Allodynia in a Pain Rat Model., Korean J Anesthesiol 60, 357, 2011 |
| 人 | Human | 肾上腺皮质 / Adrenocortical | Collagenase Type 1: 0.2%； Deoxyribonuclease I: 0.01% |
Krebs Ringer | Caroccia, B., Fassina, A., Seccia, T., Recarti, C., Petrelli, L., Belloni, A., Pelizzo, M. and Rossi, G.: Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method., Endocrinology 151, 1375, 2010 |
| 人 | Human, adult | 嗜铬细胞 / Chromaffin | Trypsin: 0.25% | Eagle's MEM | Tischler, A., DeLellis, R., Bailes, B., Nunnemacher, G.,Carabba, V., and Wolfe, H.: Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells, Lab Invest 43, 399, 1980 |
| 人 | Human, adult and child | 包皮 / Foreskin | Collagenase: 0.5% | Dulbecco's MEM w/10% calf serum | Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979 |
| 小鼠 | Mouse | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.1-0.2%； Deoxyribonuclease I: 0.003-0.015%； Hyaluronidase: 0.015% |
DMEM/F12 | Dominguez, N., Rodri茆uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012 |
| 小鼠 | Mouse, 8-10 week | 嗜铬细胞 / Chromaffin | Papain: 40 u/ml | DMEM | Kolski-Andreaco, A., Cai, H., Currle, D., Chandy, K. and Chow, R.: Mouse Adrenal Chromaffin Cell Isolation., J Vis Exp 2, 129, 2007 |
| 小鼠 | Mouse, embryonic | 染色质细胞 / Chromafin cells | Papain: 20-25 u/ml | DMEM | Tian Jin-Hua, Wu Zheng-Xing, Unzicker Michael, Lu Li, Cai Qian, Li Cuiling, Schirra Claudia, Matti Ulf, Stevens David, Deng Chuxia, Rettig Jens, Sheng Zu-Hang: The role of Snapin in neurosecretion: snapin knock-out mice exhibit impaired calcium-dependent exocytosis of large dense-core vesicles in chromaffin cells, J Neurosci 25, 10546-55, 2005 |
| 绵羊 | Ovine, adult and fetal | 肾上腺皮质 / Adrenocortical | Collagenase Type 1: 0.4% | DMEM/Ham's F12 | Valego, N. and Rose, J.: A Specific CRH Antagonist Attenuates ACTH-Stimulated Cortisol Secretion in Ovine Adrenocortical Cells., Reprod Sci 17, 477, 2010 |
| 绵羊 | Ovine, fetal | 肾上腺皮质 / Adrenocortical | Collagenase Type 1: 0.4% | DMEM/Ham's F12 | Valego, N., Su, Y., Carey, L., Young, S., Tatter, S., Wang, J. and Rose, J.: Hypothalamic-Pituitary Disconnection in Fetal Sheep Blocks the Peripartum Increases in Adrenal Responsiveness and Adrenal ACTH Receptor Expression., Am J Physiol Regul Integr Comp Physiol 289, R410, 2005 |
| 绵羊 | Ovine, adult | 嗜铬细胞 / Chromaffin cells | Collagenase Type 2: 0.2%； Deoxyribonuclease I: 100 u/ml |
Locke's solution | Keating, D., Rychkov, G., Adams, M., Holgert, H., McMillen, I.C. and Roberts, M.: Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels., J Physiol 555, 489, 2004 |
| 绵羊 | Ovine, 3 year | 垂体前叶 / Anterior pituitary | Trypsin: 2.5%； Deoxyribonuclease I: 0.004% |
DMEM | Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J: The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep, J Endocrinol 162, 215-25, 1999 |
| 大鼠 | Rat | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.1-0.2%； Deoxyribonuclease I: 0.003-0.015%； Hyaluronidase: 0.015% |
DMEM/F12 | Dominguez, N., Rodri茆uez, M., Machado, J. and Borges, R.: Preparation and Culture of Adrenal Chromaffin Cells., Methods Mol Biol 846, 223, 2012 |
| 大鼠 | Rat | 嗜铬细胞 / Chromaffin | Collagenase Type 1: 0.26%； Deoxyribonuclease I: 0.015%； Hyaluronidase: 0.015% |
HBSS | Gilabert, J, Montalvo, G, and Artalejo A.: Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays, Nat Protoc , 294, 2006 |
| 大鼠 | Rat, SD | 嗜铬细胞 / Chromaffin cells | Collagenase Type 1: 0.26%； Deoxyribonuclease I: 0.015%； Hyaluronidase: 0.015% |
HBSS | Gilabert, J: Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture,Cell Biology of the Chromaffin Cell,Borges, R. and Gandia, L., Instituto Teofilo Hernando, , 2004 |
| 大鼠 | Rat, SD, male | 束状带/网状带 / Zona fasciculata/reticularis | Collagenase: 0.4% | Krebs-HEPES | Bruder Eric D, Ball Dennis L, Goodfriend Theodore L, Raff Hershel: An oxidized metabolite of linoleic acid stimulates corticosterone production by rat adrenal cells, Am J Physiol Regul Integr Comp Physiol 284, R1631-5, 2003 |
| 大鼠 | Rat, Wistar, newborn | 嗜铬细胞 / Chromaffin cells | Collagenase Type 1: 0.025%； Deoxyribonuclease I: 0.0015% |
DMEM | Zhang L, Castell A, Avila E, Drucker-Col穩n R, Escobar A: Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons, J Neuropathol Exp Neurol 59, 170-4, 2000 |
| 大鼠 | Rat, SD, male | ZG，ZFR / ZG，ZFR | Collagenase Type 1: 0.2% | Kreb's | Sayed, S., Whitehouse, B., and Jones, P.: Phosphoserine/Threonine Phosphatases in the Rat Adrenal Cortex: A Role in the Control of Steroidogenesis, J Endocrinol 154, 449, 1997 |
| 大鼠 | Rat, Fischer, male, 10-16 weeks | 肾上腺皮质 / Adrenocortical | Deoxyribonuclease I: 0.005% | BSS | Roskelley, C.D. and Auersperg, N.: Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture, In Vitro Cell Dev Biol 26, 493, 1990 |
| 大鼠 | Rat, male, 120-160 g；Rat, SD, male, 400-450 g | Leydig，肾上腺 / Leydig，Adrenal | Collagenase Type 2: 0.03% (adrenal) | Krebs Ringer bicarbonate buffer | Ng, T. and Liu, W.: Toxic Effect of Heavy Metals on Cells Isolated from the Rat Adrenal and Testis, In Vitro Cell Dev Biol 26, 24, 1990 |
| 大鼠 | Rat, SD, 2-4 day old (also bovine) | 心脏，肾上腺嗜铬，副神经元 / Heart，Adrenal chromaffin，Paraneurons | Trypsin: 0.06% | 25mM HEPES buffered Locke's solution, CMF | Trifaro, J., Tang, R., and Novas, M.: Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons, In Vitro Cell Dev Biol 26, 335, 1990 |
| 大鼠 | Rat, Long-Evans, female, 150-200 g | 肾小球 / Glomerulosa | Collagenase: 0.2% | MEM-d-Val | Payet, N., Deziel, Y., and Lehoux, J.-G.: Vasopressin: A Potent Growth Factor in Adrenal Glomerulosa Cells in Culture, J Steroid Biochem 20, 449, 1984 |
| 大鼠 | Rat, Fischer, male, 1-10 months | 肾上腺皮质 / Adrenocortical | Deoxyribonuclease I: 0.005% | BSS | Leonard, R.K., Auersperg, N., and Parkes, C.O.: Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells, In Vitro 19, 46, 1983 |
| 大鼠 | Rat, SD, male, 400-450 g | 蜕膜，荚膜，肾小球 / Decapular，Capsular，Glomerulosa | Deoxyribonuclease I: 0.01% | Medium 199 | Li, C.H., Ng, T.B., and Cheng, C.H.K.: Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells, Int J Pept Protein Res 19, 361, 1982 |
| 大鼠 | Rat | 嗜铬细胞 / Chromaffin | Trypsin: 0.10% | Ham's F-12 w/HEPES | Englert, D.F.: An Optical Study of Isolated Rat Adrenal Chromaffin Cells, Exp Cell Res 125, 369, 1980 |
| 大鼠 | Rat, Hanover-Wistar, 2nd postnatal week (also guinea-pig, cattle) | 嗜铬细胞 / Chromaffin | Collagenase: 0.5% | HBSS | Unsicker, K., Rieffert, B., and Ziegler, W.: Effects of Cell Culture Conditions, Nerve Growth Factor, Dexamethasone, and Cyclic AMP on Adrenal Chromaffin Cells In Vitro, Adv Biochem Psychopharmacol 255, 51, 1980 |
| 大鼠 | Rat , SD, female, 200 g | 肾小球 / Glomerulosa | Deoxyribonuclease I: 0.05% | Kreb's | Braley, L., Williams, G., and Bradwin, G.: The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells, Endocrinology 106 (1), 50, 1980 |
| 大鼠 | Rat | 包皮 / Foreskin | Collagenase: 0.5% | Dulbecco's MEM w/10% calf serum | Folkman, J., Haudenschild, C. C., and Zetter, B. R.: Long-term Culture of Capillary Endothlial Cells, Proc Natl Acad Sci U S A 76, 5217, 1979 |
| 大鼠 | Rat, Wistar-Hanover, 7-12 day | 髓质 / Medullary | Trypsin: 0.125% | HBSS | Unsicker, K., Krisch, B., Otten, U., and Thoenen, H.: Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids, Proc Natl Acad Sci U S A 75 (7), 3498, 1978 |
| 大鼠 | Rat, SD, male | 皮质 / Cortical | Trypsin: 0.25% | Kreb's Ringer bicarbonate buffer | Barofsky, A., Feinstein, M., and Halkerston, I.: Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands, Exp Cell Res 79, 263, 1973 |
| 大鼠 | Rat, Holtzman, male, 180-250 g | 肾上腺 / Adrenal | Collagenase Type 1: 0.5% | Kreb's Ringer bicarbonate buffer | Kloppenborg, P., Island, D., Liddle, G., Michelakis, A., and Nicholson, W.: A Method of Preparing Adrenal Cell Suspensions and Its Applicability to the In Vitro Study of Adrenal Metabolism, Endocrinology 82, 1053, 1968 |
