组织分离/原代细胞分离，是酶在组织培养研究和细胞生物学研究中的主要应用。尽管多年来在这些应用中广泛使用酶，但它们在解离和收获中的作用机制尚不清楚。
研究人员在科学文献中搜索有关理想酶和组织解离的最佳条件的信息时，经常会遇到相互矛盾的数据。大部分变化源于细胞外基质的复杂性和动态性，以及在细胞分离应用中使用相对粗糙、不确定的酶制剂。
本指南总结了Worthington如何使用这些酶来完成对应动物脂肪/脂肪组织的解离，以及原代细胞采集的常规操作。 本技术指南描述了标准实验室程序； 为建立细胞分离方案提供了一种合乎逻辑的实验方法； 并列出了许多组织特异性参考资料，以帮助开发有效的方法。更多其他组织的解离指南，点击查看：各组织分离指南。
| 动物脂肪/脂肪 | |||||
| 种属 | 种属详情 | 细胞名称 | 酶组合推荐，含推荐浓度 | 培养基 | 参考文献 |
| 禽类 | 淀粉样蛋白原纤维
Amyloid Fibrils |
Collagenase Type 3: 0.02%； Deoxyribonuclease I: 0.001% |
50mM Tris | Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016 | |
| 禽类 | 淀粉样蛋白原纤维 / Amyloid Fibrils | Collagenase Type 3: 0.02%； Deoxyribonuclease I: 0.001% |
50mM Tris | Scherpelz, K., Lu, J.,Tycko, R. and Meredith, S.: Preparation of Amyloid Fibrils Seeded from Brain and Meninges, Methods Mol Biol 1345, 299, 2016 | |
| 熊 | Bear, Ursus arctos | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | HBSS | Gehring, J., Rigano, K., Evans, H., Nelson, O., Robbins, C. and Jansen, H.: A Protocol for the Isolation and Cultivation of Brown Bear (Ursus arctos) Adipocytes., Cytotechnology 68, 2177-91, 2016 |
| 牛 | Bovine | 脂肪细胞 / Adipocytes | Collagenase Type 1: 40 u/ml | Krebs-Ringer bicarbonate | Yang, Y., Baldwin, R.: Preparation and Metabolism of Isolated Cells from Bovine Adipose Tissue, J Dairy Sci 56, 350, 1973 |
| 犬/狗 | 23-33 kg | 基质细胞血管 / Stromal vascular | Collagenase: see reference | Media 199E | Williams, S., Kosnik, P., Kleinert, L., Vossman, E., Lye, K. and Shine, M.: Adipose Stromal Vascular Fraction Cells Isolated Using an Automated Point of Care System Improve the Patency of Expanded Polytetrafluoroethylene Vascular Grafts., Tissue Eng Part A 19, 1295-302, 2013 |
| 犬/狗 | Canine | 肾脂肪来源的细胞 / Renal adipose derived cells | Collagenase Type 1: 0.3% | DMEM | Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011 |
| 犬/狗 | Canine, 20-25 kg | 脂肪干细胞 / Adipose stem cells | Collagenase: see reference | Media-199 | Fischer, L., McIlhenny, S., Tulenko, T., Golesorkhi, N., Zhang, P., Larson, R., Lombardi, J., Shapiro, I. and DiMuzio, P.: Endothelial Differentiation of Adipose-Derived Stem Cells: Effects of Endothelial Cell Growth Supplement and Shear Force., J Surg Res 152, 157, 2009 |
| 犬/狗 | Canine | 白色脂肪 / White fat | Collagenase: 0.05% | Kreb's Ringer bicarbonate buffer | DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971 |
| 马 | Equine | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | PBS | Vidal, M., Robinson, S., Lopez, M., Paulsen, D., Borkhsenious, O., Johnson, J., Moore, R. and Gimble, J.: Comparison of Chondrogenic Potential in Equine Mesenchymal Stromal Cells Derived from Adipose Tissue and Bone Marrow., Vet Surg 37, 713, 2008 |
| 马 | Equine, 1-5 year | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | PBS | Vidal, M., Kilroy, G., Lopez, M., Johnson, J., Moore, R. and Gimble, J.: Characterization of Equine Adipose Tissue-Derived Stromal Cells: Adipogenic and Osteogenic Capacity and Comparison with Bone Marrow-Derived Mesenchymal Stromal Cells., Vet Surg 36, 613, 2007 |
| 鱼 | Fish, Atlantic salmon | 前脂肪细胞 / Preadipocytes | Collagenase Type 1: 0.1% | HBSS | Todorcevic, M., Vegusdal, A., Gjoen, T., Sundvold, H., Torstensen, B., Kjaer, M. and Ruyter, B.: Changes in Fatty Acids Metabolism During Differentiation of Atlantic Salmon Preadipocytes; Effects of n-3 and n-9 Fatty Acids., Biochim Biophys Acta 1781, 326, 2008 |
| 沙鼠 | Gerbil of unknown age (also rat, hamster, rabbit, lamb, guinea-pig) | 棕色脂肪 / Brown fat | Collagenase Type 1: 0.10% | Bicarbonate buffer | Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982 |
| 豚鼠 | Guinea-pig, adult (also rat, hamster, gerbil, rabbit, lamb) | 棕色脂肪 / Brown fat | Collagenase Type 1: 0.10% | Bicarbonate buffer | Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982 |
| 仓鼠 | Hamster, adult(also rat, gerbil, rabbit, lamb, guinea-pig) | 棕色脂肪 / Brown fat | Collagenase Type 1: 0.10% | Bicarbonate buffer | Nedergaard, J. and Lindberg, O.: The Brown Fat Cell, Int Rev Cytol 74, 187, 1982 |
| 仓鼠 | Hamster, 5 week-12 month | 白色脂肪 / White fat | Collagenase: 0.05% | Kreb's Ringer bicarbonate buffer | DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971 |
| 人 | Human | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 4: 150-300 u/ml | RPMI-1640 | Alstrup, T., Eijken, M., Bohn, A., Moller, B. and Damsgaard, T.: Isolation of Adipose Tissue-Derived Stem Cells: Enzymatic Digestion in Combination with Mechanical Distortion to Increase Adipose Tissue-Derived Stem Cell Yield from Human Aspirated Fat., Curr Protoc Stem Cell Biol 48, e68, 2019 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 2: 0.01-0.5% | DMEM | Tsurumachi, N., Akita, D., Kano, K., Matsumoto, T., Toriumi, T., Kazama, T., Oki, Y., Saito-Tamura, Y., Tonogi, M., Shimizu, N. and Honda, M.: Effect of Collagenase Concentration on The Isolation of Small Adipocytes from Human Buccal Fat Pad., J Oral Sci , , 2018 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | HBSS | Wahl, S., Drong, A., Lehne, B., Loh, M., Scott, K., Kunze, S. et al: Epigenome-wide Association Study of Body Mass Index, and the Adverse Outcomes of Adiposity, Nature 541, 81, 2017 |
| 人 | Human | 基质细胞 / Stromal | Collagenase Type 2: 0.075% | DMEM | Duscher, D., Maan, Z., Luan, A., Aitzetmuller, M., Brett, E., Atashroo, D., Whittam, A., Hu, M., Walmsley, G., Houschyar, K., Schilling, A., Machens, H., Gurtner, G., Longaker, M. and Wan, D.: Ultrasound-Assisted Liposuction Provides a Source for Functional Adipose-Derived Stromal Cells., Cytotherapy 19, 1491-1500, 2017 |
| 人 | Human | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 1: 0.075% | PBS | Lin, Y., Marin-Argany, M., Dick, C., Redhage, K., Blancas-Mejia, L., Bulur, P., Butler, G., Deeds, M., Madden, B., Williams, A., Wall, J., Dietz, A. and Ramirez-Alvarado, M.: Mesenchymal Stromal Cells Protect Human Cardiomyocytes from Amyloid Fibril Damage, Cytotherapy 19, 1426-1437, 2017 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 2: 0.2% | DMEM/F-12 | Munir, H., Ward, L., Sheriff, L., Kemble, S., Nayar, S., Barone, F., Nash, G. and McGettrick, H.: Adipogenic Differentiation of Mesenchymal Stem Cells Alters Their Immunomodulatory Properties in a Tissue-Specific Manner., Stem Cells 35, 1636-1646, 2017 |
| 人 | Human | 脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem | Collagenase animal free: 0.1% | PBS | Clark, K., Fierro, F., Ko, E., Walker, N., Arzi, B., Tepper, C., Dahlenburg, H., Cicchetto, A., Koi, A., Marsh, L., Murphy, W., Fazel, N., and Borjesson, D.: Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome., Stem Cell Res Ther 8, 69, 2017 |
| 人 | Human | 脂肪来源的内皮 / Adipose derived endothelial | Collagenase Type 1: 0.1% | DMEM | Szoke, K., Reinisch, A., Ostrup, E., Reinholt, F. and Brinchmann, J.: Autologous Cell Sources in Therapeutic Vasculogenesis: In Vitro and In Vivo Comparison of Endothelial Colony-Forming Cells from Peripheral Blood and Endothelial Cells Isolated from Adipose Tissue., Cytotherapy 18, 242-52, 2016 |
| 人 | Human | 脂肪干细胞 / Adipose stem | Collagenase Type 1: 0.1% | DMEM/F12 | Cheng, N., Hsieh, T., Lai, H. and Young, T.: High Glucose-Induced Reactive Oxygen Species Generation Promotes Stemness in Human Adipose-Derived Stem Cells, Cytotherapy 18, 371-83, 2016 |
| 人 | Human | 牙髓干细胞 / Dental pulp stem | Collagenase animal free: 0.1% | HBSS | Hu, J., Cao, Y., Xie, Y., Wang, H., Fan, Z., Wang, J., Zhang, C., Wang, J., Wu, C. aand Wang, S.: Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice., Stem Cell Res Ther 7, 130, 2016 |
| 人 | Human | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 4: 0.2% | PBS | Choudhery,M, Badowski, M, Muise, A and Harris, D: Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells., Cytotherapy 17, 359-68, 2015 |
| 人 | Human | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 2: 0.1% | HBSS | Satish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.: Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage., BMC Med Genomics 8, 41, 2015 |
| 人 | Human, female | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.075% | DMEM | Kinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., Kurisaki, A. and Yoshimura, K.: Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers., Stem Cells Transl Med 4, 146, 2015 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | PBS | Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015 |
| 人 | Human | 脂肪基质细胞-血管 / Adipose stroma-vascular | Neutral Protease: 2.4 u/ml； Collagenase: 250 u/ml |
PBS | Esteve, D., Boulet, N., Volat, F., Zakaroff-Girard, A., Ledoux, S., Coupaye, M., Decaunes, P., Belles, C., Gaits-Iacovoni, F., Iacovoni, J., Remaury, A., Castel, B., Ferrara, P., Heymes, C., Lafonta, M. Boloumie, J.: Human White and Brite Adipogenesis is Supported by MSCA1 and is impaired by Immune Cells., Stem Cells 33, 1277-91, 2015 |
| 人 | Human, adult | 脂肪来源的间充质干细胞 / Adipose derived mesenchymal stem | Collagenase Type 2: 0.1% | see reference | Al-Saqi, S., Saliem, M., Asikainen, S., Quezada, H., Ekblad, A., Hovatta, O., Le Blanc, K., Jonasson, A. and Gotherstrom, C.: Defined Serum-Free Media for In Vitro Expansion of Adipose-Derived Mesenchymal Stem Cells., Cytotherapy 16, 915, 2014 |
| 人 | Human, adult | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.15% | DMEM | Koellensperger, E., Bollinger, N., Dexheimer, V., Gramley, F., Germann, G. and Leimer, U.: Choosing the Right Type of Serum for Different Applications of Human Adipose Tissue-Derived Stem Cells: Influence on Proliferation and Differentiation Abilities., Cytotherapy 16, 789, 2014 |
| 人 | Human | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 1: 0.1% | PBS | Najar, M, Rodrigues, R, Buyl, K, Branson, S, Vanhaecke, T, Lagneaux, L, Rogiers, V and De Kock, J: Proliferative and Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods., Cytotherapy 16, 1220-8, 2014 |
| 人 | Human | 脂肪基质细胞干细胞 / Adipose stromal stem | Collagenase animal free: 200 u/ml | DMEM/Hams F-12 | Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.: Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells., Tiss Eng 19, 473-8, 2013 |
| 人 | Human | 基质细胞血管部分 / Stromal vascular fraction | Collagenase Type 1: 0.075% | PBS | Doi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.: Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis., J Tissue Eng Regen Med 7, 864, 2013 |
| 人 | Human | 脂肪来源的干细胞 / Adipose-derived stem | Collagenase Type 1: 0.1% | PBS | Cervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.: Platelet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance., Stem Cells Transl Med 1, 206-20, 2012 |
| 人 | Human | 血管周围干细胞 / Perivascular stem | Collagenase Type 2: 0.1% | DMEM | James, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.: An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering., Stem Cells Transl Med 1, 673, 2012 |
| 人 | Human | 脂肪来源的基质细胞血管 / Adipose derived stromal vascular | Collagenase Type 1: 0.1% | PBS | Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.: Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery., Stem Cells Transl Med 1, 230-6, 2012 |
| 人 | Human | 基质细胞血管，脂肪细胞 / Stromal vascular, adipocytes | Collagenase Type 1: 0.1% | DMEM | Gentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.: A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Platelet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction., Stem Cells Transl Med 1, 341-51, 2012 |
| 人 | Human, adult | 基质细胞血管 / Stromovascular | Collagenase Type 1: 0.1% | PBS with BSA | Hagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.: Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing., J Immunol Methods 386, 50, 2012 |
| 人 | Human | 脂肪来源的基质细胞 / Adipose derived stromal | Collagenase Type 1: 0.1% | PBS | Naaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.: Human Platelet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications., Cell Tissue Res 348, 119, 2012 |
| 人 | Human | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.1% | DMEM/F-12 | Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.: An Injectable Adipose Matrix for Soft-Tissue Reconstruction., Plast Reconstr Surg 129, 1247, 2012 |
| 人 | Human, male 40-60 years | 脂肪干细胞 / Adipose derived stem cells | Collagenase: 0.25%； Deoxyribonuclease I: 0.002% |
PBS | Blasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.: Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential, Vasc Cell 3, 5, 2011 |
| 人 | Human | 肾脂肪来源的细胞 / Renal adipose derived cells | Collagenase Type 1: 0.3% | DMEM | Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011 |
| 人 | Human | 脂肪来源的间充质 / Adipose derived mesenchymal | Collagenase Type 1: 0.1% | L-DMEM | Yang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.: High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells., J Biomed Sci 18, 59, 2011 |
| 人 | Human | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.1% | DMEM/Ham's F-12 | Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.: Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates., Methods Mol Biol 702, 17-27, 2011 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | RPMI | Basu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.: Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation., Obesity 19, 476, 2011 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | KRB | Peters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.: Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture., PLoS ONE 5, e15689, 2010 |
| 人 | Human | 脂肪组织来源的干细胞 / Adipose tissue-derived stem | Collagenase Type 2: 1.0% | DMEM/F12 | Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.: Novel Multiarm PEG-Based Hydrogels for Tissue Engineering., J Biomed Mater Res 92, 979, 2010 |
| 人 | Human | 基质细胞血管 / Stromal vascular | Collagenase Type 2: 0.1% | HBSS | Zimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.: Stromal Vascular Progenitors in Adult Human Adipose Tissue., Cytometry 77, 22-30, 2010 |
| 人 | Human | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.1% | DMEM/Hams F-12 | Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.: Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes., Cytotherapy 12, 538, 2010 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase: 0.075% | DMEM | Suga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.: IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism., Stem Cells 27, 238, 2009 |
| 人 | Human | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.1% | DMEM | Cai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.: IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function., Stem Cells 27, 230, 2009 |
| 人 | Human | 脂肪组织来源的干细胞 / Adipose tissue-derived stem | Collagenase Type 2: 1.0% | DMEM/F12 | Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.: Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering., Biomaterials 30, 6844, 2009 |
| 人 | Human | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | DMEM | Tandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.: Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation., Conf Proc IEEE Eng Med Biol Soc 2009, 6517-21, 2009 |
| 人 | Human | 脂肪来源的基质细胞 / Adipose derived stromal cells | Collagenase Type 1: see reference | DMEM | Traktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.: A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks, Circ Res 102, 77, 2008 |
| 人 | Human | 基质细胞 / Stromal | Collagenase Type 1: 0.2% | PBS | Minana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.: IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue., Stem Cells 26, 2696, 2008 |
| 人 | Human | 基质细胞 / Stromal | Collagenase Type 1: 0.1%； Neutral Protease: |
DMEM | Nie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.: IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells., Stem Cells 26, 2735, 2008 |
| 人 | Human | 干细胞 / Stem | Collagenase: 280 u/ml | D-PBS | Pilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.: Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells., Regen Med 3, 705-15, 2008 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.2% | HBSS | Bujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha: Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat, J Endocrinol 192, 279-88, 2007 |
| 人 | Human | 脂肪来源的基质细胞 / Adipose derived stromal cells | Collagenase Type 1: 0.15% | DMEM | Schaffler A, Buchler C: Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies, Stem Cells 25, 818-27, 2007 |
| 人 | Human | 脂肪干细胞 / Adipose derived stem cells | Collagenase Type 1: 0.1% | DMEM/F12 | Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.: Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors., J Cell Physiol 212, 702-9, 2007 |
| 人 | Human | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.1% | HBSS | Jeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho: Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK, J Lipid Res 47, 653-64, 2006 |
| 人 | Human | 前脂肪细胞 / Preadipocytes | Collagenase Type 1: 196 u/ml | M199 | Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.: Human Serum from Platelet-Poor Plasma for the Culture of Primary Human Preadipocytes., Stem Cells 24, 1218-25, 2006 |
| 人 | Human | 脂肪细胞，基质细胞血管 / Adipocytes, stromal vascular | Collagenase: 0.2% | HBSS | Boquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E: Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture, Mol Biol Cell 16, 1131-41, 2005 |
| 人 | Human | 多能脂肪来源的干细胞 / Multipotent adipose derived stem | Collagenase: 0.2% | DMEM | Rodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian: Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse, J Exp Med 201, 1397-405, 2005 |
| 人 | Human | 成体干细胞 / Adult stem cells | Collagenase Type 1: 0.1% | PBS | Devireddy, R., Thirumala, S. and Gimble, J.: Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress., J Biomech Eng 127, 1081, 2005 |
| 人 | Human, adult, obese | 脂肪细胞 / Adipocytes | Collagenase Type 2: 0.1% | DMEM/F12 | Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.: Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation, J Clin Endocrinol Metab 89(10), 4833, 2004 |
| 人 | Human, adult, female | 前脂肪细胞 / Preadipocytes | Collagenase Type 1: 0.2% | DMEM/F-12 | Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,: Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5, J Endocrinol 183, 331, 2004 |
| 人 | Human, adult | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.13% | see reference | Fain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.: Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans, Endocrinology 145, 2273, 2004 |
| 人 | Human | 基质细胞血管，脂肪细胞 / Stromal vascular, adipocytes | Collagenase: 0.2% | DMEM/F12 | Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004 |
| 人 | Human | 基质细胞血管，脂肪细胞，干细胞 / Stromal vascular, adipocytes, stem | Collagenase: 300 u/ml | PBS | Miranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A: Improvement of postnatal neovascularization by human adipose tissue-derived stem cells, Circulation 110, 349-55, 2004 |
| 人 | Human, adult, female, non-obese | 脂肪细胞 / Adipocytes | Collagenase Type 2: 0.05% | DMEM | Gesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.: Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression, Horm Metab Res 35, 158, 2003 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: see reference | Saline | Patwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.: Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery, Ped Neurosurg 36, 314, 2002 |
| 人 | Human, adult, male and female | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.2% | DMEM/F12 | McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.: Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences, J Clin Endocrinol Metab 87, 1327, 2002 |
| 人 | Human | 处理过的脂肪抽吸细胞 / Processed lipoaspirate cells | Collagenase Type 1: 0.075% | PBS | Zuk, P., Zhu, M., Ashjian, P., De Ugarte, D., Huang, J., Mizuno, H., Alfonso, Z., Fraser, J., Benhaim, P., and Hedrick, M.: Human adipose tissue is a source of multipotent stem cells, Mol Biol Cell 13, 4279-95, 2002 |
| 人 | Human | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.2% | HBSS | McTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S: Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue, Int J Obes Relat Metab Disord 24, 875-81, 2000 |
| 人 | Human, adult, male and female | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | DMEM/Ham's F-12 | Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.: Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1, Eur J Endocrinol 141 (4), 436, 1999 |
| 人 | Human, adult, male and female | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | DMEM/F12 | Zhang, H., Kumar, S., Barnett, A., and Eggo, M.: Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake, J Clin Endocrinol Metab 84, 2550, 1999 |
| 人 | Human, non-diabetic, male | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.05% | Kreb's Ringer bicarbonate buffer | Anderson, O., Gliemann, J., and Gammeltoft: Receptor Binding and Biological Effect of Insulin in Human Adipocytes, Diabetologia 13, 589, 1977 |
| 小鼠 | Mouse | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | DMEM | Jones, J., Rabhi, N., Orofino, J., Gamini, R., Perissi, V., Vernochet, C. and Farmer, S.: The Adipocyte Acquires a Fibroblast-Like Transcriptional Signature in Response to a High Fat Diet., Sci Rep 10, 2380, 2020 |
| 小鼠 | Mouse | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.1% | HBSS | Bowles, A., Strong, A., Wise, R., Thomas, R., Gerstein, B., Dutreil, M., Hunter, R., Gimble, J. and Bunnell, B.: Adipose Stromal Vascular Fraction-Mediated Improvements at Late-Stage Disease in a Murine Model of Multiple Sclerosis., Stem Cells 35, 532-544, 2017 |
| 小鼠 | Mouse | 脂肪间质基质细胞 / Adipose mesenchyal stromal | Collagenase Type 2: 0.2% | DMEM | Maria, O., Shalaby, M., Syme, A., Eliopoulos, N. and Muanza, T.: Adipose Mesenchymal Stromal Cells Minimize and Repair Radiation-Induced Oral Mucositis., Cytotherapy 18, 1129-45, 2016 |
| 小鼠 | Mouse | 脂肪基质细胞 / Adipose stromal | Collagenase Type 1: 0.1% | PBS | Yao, W., Lay, Y., Kot, A., Liu, R., Zhang, H., Chen, H., Lam, K. and Lane, N.: Improved Mobilization of Exogenous Mesenchymal Stem Cells to Bone for Fracture Healing and Sex Difference., Stem Cells 34, 2587-2600, 2016 |
| 小鼠 | Mouse | 脂肪细胞 / Adipocytes | Collagenase: 0.1% | Krebs | Jang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.: The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life., Endocrinology 156, 3737, 2015 |
| 小鼠 | Mouse | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.1% | PBS | Seaman, S., Tannan, S., Cao, Y., Peirce, S. and Lin, K.: Differential Effects of Processing Time and Duration of Collagenase Digestion on Human and Murine Fat Grafts., Plast Reconstr Surg 136, 189e-199e, 2015 |
| 小鼠 | Mouse, 20 week | 基质细胞血管 / Stromal vascular | Collagenase Type 2: 0.2% | RPMI-1640 | Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.: Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice., Exp Anim 62, 319, 2013 |
| 小鼠 | Mouse | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 2: 0.1% | DMEM | Takahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.: Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair., Stem Cells Transl Med 1, 803, 2012 |
| 小鼠 | Mouse | 干细胞和祖 / Stem and progenitor | Collagenase Type 2: 0.2% | HBSS | Han, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.: Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells., Blood 115, 957, 2010 |
| 小鼠 | Mouse, 3 week | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.2% | HBSS | De Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbatelli, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.: In Vivo Physiological Transdifferentiation of Adult Adipose Cells., Stem Cells 27, 2761, 2009 |
| 小鼠 | Mouse | 白色脂肪细胞 / White adipocytes | Collagenase Type 2: 0.1% | DMEM | Wong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y: Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins., Am J Physiol/Endo 296, 820, 2009 |
| 小鼠 | Mouse | 脂肪基质细胞 / Adipose stromal | Collagenase Type 2: 0.075% | DMEM | Thangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.: IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia., Stem Cells 27, 266, 2009 |
| 小鼠 | Mouse | 脂肪组织来源的干细胞 / Adipose tissue-derived stem | Collagenase Type 1: 0.075% | Modified Eagles | Cho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.: IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model., Stem Cells 27, 259-65, 2009 |
| 小鼠 | Mouse, C57Bl/6J | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.15% | DMEM/F12 | Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes, J Health Sci 51, 21, 2005 |
| 小鼠 | Mouse, 3-6 day and 2-3 month | 脂肪来源的基质细胞 / Adipose-derived stromal cells | Collagenase Type 2: 0.075% | PBS | Cowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T: Adipose-derived adult stromal cells heal critical-size mouse calvarial defects, Nat Biotechnol 22, 560-7, 2004 |
| 小鼠 | Mouse | 基质细胞血管，脂肪细胞 / Stromal vascular, adipocytes | Collagenase: 0.2% | DMEM/F12 | Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis: Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives, Circulation 109, 656-63, 2004 |
| 小鼠 | Mouse, C57BL/6J and FVB, male, 8-9 week | 脂肪细胞 / Adipocytes | Collagenase: see reference | DMEM | Ruan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.: Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes, J Biol Chem 278, 47585, 2003 |
| 小鼠 | Mouse, both sexes | 脂肪细胞 / Adipocytes | Collagenase: 0.05% | Krebs-Ringer Phosphate HEPES (KRPH) | Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.: Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice, Am J Physiol/Endo 281, E1249, 2001 |
| 小鼠 | Mouse, B6D2F1, F1 hybrids, New Zealand black female & New Zealand white male | 血管内皮 / Vascular endothelial | Collagenase: 0.2% | PBS | Launder, T., Gegen, N., Knedler, A., and Harbeck, R.: The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue, J Immunol Methods 102, 45, 1987 |
| 猪 | Porcine, female, <1 year | 脂肪间充质干细胞 / Adipose mesenchymal stem | Collagenase Type 1: 0.1% | DMEM | Williams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K: Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells., Cells Tissues Organs 188, 251, 2008 |
| 猪 | Porcine, crossbred, male 1-4 day | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.2% | DMEM/F12 | Ramsay, T.G.: Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes, J Anim Sci 81, 3008, 2003 |
| 猪 | Porcine, crossbred | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.067% | Krebs-Ringer | Liang, W. and Mills, S.: Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues., J Anim Sci 80, 963-70, 2002 |
| 猪 | Porcine, 8-9 week | 脂肪 / Adipose | Collagenase Type 1: 300 u/ml | HEPES | Ding, S., McNeel, R., and Mersmann, H.: Expression of Porcine Adipocyte Transcripts: Tissue Distribution and Differentiation In Vitro and In Vivo, Comp Biochem Physiol B 123, 307, 1999 |
| 猪 | Porcine, neonatal | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.3% | Krebs-Ringer bicarbonate albumin | Wang Y, Fried SK, Petersen RN, Schoknecht PA: Somatotropin regulates adipose tissue metabolism in neonatal swine, J Nutr 129, 139-45, 1999 |
| 猪 | Porcine, crossbred, 1-3 day | 脂肪，基质细胞血管 / Adipose, Stromal-vascular | Collagenase Type 1: 0.2% | DMEM/F12 | Suryawan, A., Swanson, L., and Hu, C.: Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture, J Anim Sci 75, 105, 1997 |
| 大鼠 | Rat, SD, 2-3 month | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 1: 0.1% | DMEM | Dayer, D., Tabar, M., Moghimipour, E., Tabandeh, M., Ghadiri, A., Bakhshi, E., Orazizadeh, M. and Ghafari, M.: Sonic Hedgehog Pathway Suppression and Reactivation Accelerates Differentiation of Rat Adipose-Derived Mesenchymal Stromal Cells Toward Insulin-Producing Cells, Cytotherapy 19, 937-946, 2017 |
| 大鼠 | Rat, 9 week | 脂肪基质细胞 / Adipose stromal | Collagenase: 0.1% | DMEM | Ohta, Y., Hamaguchi, A., Ootaki, M., Watanabe, M., Takeba, Y., Iiri, T., Matsumoto, N. and Takenaga, M.: Intravenous Infusion of Adipose-Derived Stem/Stromal Cells Improves Functional Recovery of Rats with Spinal Cord Injury., Cytotherapy 19, 839-848, 2017 |
| 大鼠 | Rat | 间充质干细胞 / Mesenchymal stem | Collagenase Type 1: 0.075% | DMEM | Emre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E: Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model., Cytotherapy 17, 543-59, 2015 |
| 大鼠 | Rat, SD, 5 month | 间充质基质细胞 / Mesenchymal stromal | Collagenase Type 2: 0.075% | DMEM | Veronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M: In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells., Cytotherapy 17, 215-23, 2015 |
| 大鼠 | Rat | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.3% | Krebs-Ringer | Ogasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.: Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes., Biochem Biophys Res Commun 464, 348-53, 2015 |
| 大鼠 | Rat, adult | 基质细胞血管 / Stromal vascular | Collagenase Type 1: 0.2% | PBS | Leblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.: Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct., Stem Cells Transl Med 2, 896-905, 2013 |
| 大鼠 | Rat, male, 300g | 脂肪细胞 / Adipocytes | Collagenase Type 2: 0.33% | RPMI 1640 | Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.: Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue., J Lipid Res 53, 2797, 2012 |
| 大鼠 | Rat, Lewis, male | 肾脂肪来源的细胞 / Renal adipose derived cells | Collagenase Type 1: 0.3% | DMEM | Basu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.: Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney., Lipids Health Dis 10, 171, 2011 |
| 大鼠 | Rat, SD, neonatal | 棕色脂肪细胞 / Brown adipocytes | Collagenase Type 4: 0.1%； Neutral Protease: 0.1%； Trypsin: 0.05% |
PBS | Liu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.: Efficient Isolation of Cardiac Stem Cells from Brown Adipose., J Biomed Biotechnol 2010, 104296, 2010 |
| 大鼠 | Rat | 脂肪来源的干细胞 / Adipose derived stem | Collagenase Type 1: 0.1% | DMEM | Wei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.: IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats., Stem Cells 27, 478, 2009 |
| 大鼠 | Rat | 脂肪组织来源的干细胞 / Adipose tissue-derived stem | Collagenase Type 2: 0.075% | DMEM | Tomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O'Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.: Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue., Stem Cells 26, 330, 2008 |
| 大鼠 | Rat, Wistar, 4 week | 脂肪细胞 / Adipocytes | Collagenase: 0.2% | Ham's F12 | Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.: Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro, Cell Struct Funct 28, 55, 2003 |
| 大鼠 | Rat, SD | 脂肪细胞 / Adipocytes | Collagenase Type 1: 0.2% | KRHB | Mora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J: The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency, Endocrinology 142, 1999, 2001 |
| 大鼠 | Rat, SD, male, 4-7 weeks | 棕色脂肪细胞 / Brown adipocytes | Deoxyribonuclease I: 0.5% | DMEM | Omatsu-Kanbe, M., and Matsuura, H.: Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes, J Physiol 521 (3), 601, 1999 |
| 大鼠 | Rat | 脂肪细胞 / Adipocytes | Collagenase Type 2: 0.2% | DMEM /F-12 | Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell & Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995 |
| 大鼠 | Rat, SD, male, 130-160 g | 脂肪，附睾脂肪垫 / Adipose，Epididymal fat pads | Collagenase: 0.3% | Kreb's-Ringer bicarbonate buffer modified | Charron, M.J. and Kahn, B.B.: Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose Cells In Vivo, J Biol Chem 265, 7994, 1990 |
| 大鼠 | Rat, 3 day | 前脂肪细胞 / Preadipocytes | Collagenase Type 3: 0.10% | Parker Medium 199 | Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.: Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture, Biochim Biophys Acta 968, 231, 1988 |
| 大鼠 | Rat, SD, various weights and ages | 棕色脂肪细胞，肩胛间和宫颈贮库 / Brown adipocytes，Interscapular & cervical depots | Collagenase: 0.2%； Soybean Trypsin Inhibitor: 0.3% |
Krebs Ringer bicarbonate buffer | Woodward, Julie A. and Saggerson, E.: Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline, Biochem J 238, 395, 1986 |
| 大鼠 | Rat, CD, male, 150-200 g | 脂肪细胞，附睾脂肪垫 / Adipocytes，Epididymal fat pads | Collagenase: 0.1% | Krebs Ringer bicarbonate buffer | Pessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.: ß-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes, J Biol Chem 258, 7386, 1983 |
| 大鼠 | Rat, Wistar, albino, male, 100-140 g | 脂肪细胞，附睾脂肪垫 / Adipocytes，Epididymal-fat pads | Collagenase: 0.3% | Kreb's Ringer | Green, A. and Newsholme, E.: Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites, Biochem J 180, 365, 1979 |
| 大鼠 | Rat, Fischer, 344, male, 9 - 13 week | 白色脂肪 / White fat | Collagenase: 0.3% | Kreb's Ringer bicarbonate buffer | Stiles, J.W., Francendese, A.A. and Masoro, E.J.: Influence of Age on Size and Number of Fat Cells in the Epididymal Depot, Am J Physiol 229, 1561, 1975 |
| 大鼠 | Rat, Wistar, male, 5 week-16 month | 白色脂肪 / White fat | Collagenase: 0.05% | Kreb's Ringer bicarbonate buffer | DiGirolamo, M., Mendlinger, S., and Fertig, J.W.: A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species, Am J Physiol 221, 850, 1971 |
| 大鼠 | Rat (CFE), albino, female | 棕色脂肪 / Brown fat | Collagenase Type 1: 0.10% | Bicarbonate buffer | Fain, J., Reed, N., and Saperstein, R.: Isolation and Metabolism of Brown Fat Cells, J Biol Chem 242, 1887, 1967 |
| 大鼠 | Rat, SD, male, 160-210 g | 胖的 / Fat | Collagenase: 0.3% | Albumin-bicarbonate buffer | Rodbell, M.: Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis, J Biol Chem 239 (2), 375, 1964 |
| 松鼠 | Squirrel | 棕色脂肪细胞 / Brown adipocytes | Collagenase Type 2: 0.16% | Krebs-Ringer phosphate | McFarlane, S., Mathers, K. and Staples, J.: Reversible Temperature-Dependent Differences in Brown Adipose Tissue Respiration During Torpor in a Mammalian Hibernator., Am J Physiol Regul Integr Comp Physiol 312, R434-R442, 2017 |
