双链(ds)RNA的形成是病毒感染的标志，对于诱导先天免疫至关重要。dsRNA还参与基因沉默，并在体外转录基因合成过程中作为副产物产生。抗dsRNA单克隆抗体是在细胞培养和组织中检测dsRNA的有效工具，主要应用于如下领域：
1.用于表征和检测具有dsRNA基因组或中间体的病毒（包括SARS，丙型肝炎，登革热或西尼罗河病毒）。
2.作为诊断工具，用于确定未知病原体是病毒还是细菌来源
3.用于体外转录(m)RNA制备的质量控制
dsRNA抗体精选与推荐：
| 货号 | 产品 | 描述 | 选择指南 |
| RNT-SCI-10010500 | 抗 dsRNA 单克隆抗体 J2 | 小鼠，IgG2a，κ 轻链 | IVT体外转录质控，金标准；文章最多 |
| RNT-SCI-10040500 | 抗 dsRNA 单克隆抗体 J5 | 小鼠，IgG2b，κ 轻链 | 更适合IF实验 |
| RNT-SCI-10020500 | 抗 dsRNA 单克隆抗体 K1 | 小鼠，IgG2a，κ 轻链 | 更适合 Poly I:C 检测 |
| RNT-SCI-10050100 | 抗 dsRNA ，抗体套装 | J2、J5 和 K1 抗体的尺寸样本 | 推荐用于 J2、J5 和 K1 抗体的比较测试 |
| RNT-SCI-10030010 | 抗 dsRNA 单克隆抗体 K2 | 小鼠，IgM，κ 轻链 | 更适合ELISA |
| RNT-SCI-10080100 | dsRNA 142 bp | 合成 dsRNA | 阳性对照 |
Fig.1：A）使用抗双链RNA单克隆抗体J2在SARS-CoV感染的Vero细胞中检测dsRNA的免疫荧光。 1：未感染的细胞。 标尺：20微米（根据[4]进行修改）。 B）使用抗双链RNA单克隆抗体J2在SARS-Cov感染的Vero E6细胞的双膜囊泡（DMVs）中进行免疫电子显微镜检测（根据[5]进行修改）。 C）使用抗双链RNA单克隆抗体J2在柯萨奇病毒感染的新生小鼠FFPE组织中检测dsRNA。 1：小鼠心脏，2：褐色脂肪，3：胰腺，4：唾液腺，5：中枢神经系统神经节，6：未感染组织。
《Cell文章推荐》：
SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9，Cell | 2023 Oct 26
Article Snippet
".. washed once with ice-cold PBS, followed by two washes with PBS at room temperature.. For combining immunostaining with HCR-based detection, we used anti-J2 antibody (Jena Bioscience, RNT-SCI-10010200, 1:500) or anti-SND1 antibody (Proteintech, 60265-1- Ig, 1:500).. Incubation with the primary antibody was performed for 1 h at room temperature.Incubation with .."
Figure Legend
"... (B) Representative images of IF staining for dsRNA (J2 antibody) using HCR detection in SARS-CoV-2 infected"
更多历史参考文献：
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[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255.
[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford
[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059.
[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.
[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180.
[7] Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.
