货号 产品 描述 选择指南
RNT-SCI-10010500 抗 dsRNA 单克隆抗体 J2 小鼠,IgG2a,κ 轻链 IVT体外转录质控,金标准;文章最多
RNT-SCI-10040500 抗 dsRNA 单克隆抗体 J5 小鼠,IgG2b,κ 轻链 更适合IF实验
RNT-SCI-10020500 抗 dsRNA 单克隆抗体 K1 小鼠,IgG2a,κ 轻链 更适合 Poly I:C 检测
RNT-SCI-10050100 抗 dsRNA ,抗体套装 J2、J5 和 K1 抗体的尺寸样本 推荐用于 J2、J5 和 K1 抗体的比较测试
RNT-SCI-10030010 抗 dsRNA 单克隆抗体 K2 小鼠,IgM,κ 轻链 更适合ELISA
RNT-SCI-10080100 dsRNA 142 bp 合成 dsRNA 阳性对照

Fig.1:A)使用抗双链RNA单克隆抗体J2在SARS-CoV感染的Vero细胞中检测dsRNA的免疫荧光。 1:未感染的细胞。 标尺:20微米(根据[4]进行修改)。 B)使用抗双链RNA单克隆抗体J2在SARS-Cov感染的Vero E6细胞的双膜囊泡(DMVs)中进行免疫电子显微镜检测(根据[5]进行修改)。 C)使用抗双链RNA单克隆抗体J2在柯萨奇病毒感染的新生小鼠FFPE组织中检测dsRNA。 1:小鼠心脏,2:褐色脂肪,3:胰腺,4:唾液腺,5:中枢神经系统神经节,6:未感染组织。



SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9,Cell  |  2023 Oct 26

Article Snippet

".. washed once with ice-cold PBS, followed by two washes with PBS at room temperature.. For combining immunostaining with HCR-based detection, we used anti-J2 antibody (Jena BioscienceRNT-SCI-10010200, 1:500) or anti-SND1 antibody (Proteintech, 60265-1- Ig, 1:500).. Incubation with the primary antibody was performed for 1 h at room temperature.Incubation with .."

Figure Legend
"... (B) Representative images of IF staining for dsRNA (J2 antibody) using HCR detection in SARS-CoV-2 infected"



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[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods47: 255.

[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford

[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80(10): 5059.

[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6(9): e226.

[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 180.

[7] Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA. Nucleic Acids Research 39(21): e142.



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