如何真正准确可靠地检测腺病毒(adenovirus)滴度?

 

【前情提示】

1. 慢病毒滴度检测

2.腺相关病毒(AAV)滴度检测

 

腺病毒(Adenovirus)是一种没有包膜的直径为90-100 nm的颗粒,由252个壳粒呈20面体排列构成。每个壳粒的直径为7~9 nm。衣壳里是线状双链DNA分子,约含4.7kb。腺病毒最常引起呼吸道疾病,从普通感冒到肺炎、哮吼和支气管炎。根据类型,腺病毒可引起其他疾病,如胃肠炎、结膜炎、膀胱炎,以及较少见的神经系统疾病。

腺病毒

可能很多人第一次听到腺病毒还是因为咱们陈薇院士团队开发的,唯一可采用单针接种程序的疫苗:腺病毒载体疫苗。腺病毒载体疫苗,是指以腺病毒作为载体,将保护性抗原基因重组到腺病毒基因组中,使用能表达保护性抗原基因的重组腺病毒制成的疫苗。它是一种不同于传统疫苗和mRNA疫苗的另外一条技术路线!

 

那么如何真正准确可靠地检测腺病毒(adenovirus)滴度呢?是从事腺病毒科研和疫苗研发的工作者非常关心的问题!

 

作为专业的生命科学医药原料供应商,艾美捷科技为您推荐:下面我们就来聊下几种常见的腺病毒滴度的检测方法。

货号 名称 规格 说明
VPK-110 QuickTiter™ Adenovirus Titer ELISA Kit
QuickTiter 腺病毒滴度ELISA检测试剂盒
2 x 96 assays 【2.5天获得结果,远超传统PFU空斑所需的10天】
#以ELISA原理,基于标准品,读取吸光值。
VPK-109 QuickTiter™ Adenovirus Titer Immunoassay Kit
QuickTiter 腺病毒滴度IHC检测试剂盒
100 assays 【2.5天获得结果,远超传统PFU空斑所需的10天】
#以IHC形式呈现结果,计数细胞。
VPK-106 QuickTiter™ Adenovirus Quantitation Kit
QuickTiter 腺病毒核酸滴度检测试剂盒
20 assays 【极速,45-60分钟获得结果】
#基于核酸染料与病毒DNA结合,产生荧光信号进行检测腺病毒DNA量

 

① VPK-110, 腺病毒滴度ELISA检测试剂盒

提供了一个快速、完整的系统,以功能滴度病毒感染。该检测方法识别所有41种血清型腺病毒,可用于任何能在hek293细胞中扩增的腺病毒系统。

 

VPK-110 标曲

VPK-110 标曲

 

 

发表文章,VPK-110, 腺病毒滴度ELISA检测试剂盒

1.Teves, M.E. et al. (2020). Human DENND1A.V2 Drives Cyp17a1 Expression and Androgen Production in Mouse Ovaries and Adrenals. Int J Mol Sci. 21(7). pii: E2545. doi: 10.3390/ijms21072545.

2.Guo, Y. et al. (2019). Menstrual blood-derived stem cells as delivery vehicles for oncolytic adenovirus virotherapy for colorectal cancer. Stem Cells Dev. doi: 10.1089/scd.2018.0222.

3.Wu, Y. et al (2017). Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice. Antiviral Research. 147: 29-36.

4.Kirshmer, N. et al. (2016). TRPC4a and TRPC4ß similarly affect neonatal cardiomyocyte survival during chronic GPCR stimulation. PLoS One 11:e0168446.

5.García-Pascual, C. M. et al. (2015). Evaluation of the potential therapeutic effects of a double-stranded RNA mimic complexed with polycations in an experimental mouse model of endometriosis. Fertil Steril. doi:10.1016/j.fertnstert.2015.07.1147.

 

② VPK-109, 腺病毒滴度IHC检测试剂盒

提供了一个快速、完整的系统,以功能滴度病毒感染。该检测方法识别所有41种血清型腺病毒,可用于任何能在hek293细胞中扩增的腺病毒系统。

VPK-109, 腺病毒滴度IHC检测试剂盒

发表文章,VPK-109, 腺病毒滴度IHC检测试剂盒:

1.McQuerry, J.A. et al. (2021). Tepoxalin increases chemotherapy efficacy in drug-resistant breast cancer cells overexpressing the multidrug transporter gene ABCB1. Transl Oncol. 14(10):101181. doi: 10.1016/j.tranon.2021.101181.

2.Tournier, B.B. et al. (2021). Amyloid and Tau Induce Cell Death Independently of TSPO Polymerization and Density Changes. ACS Omega. doi: 10.1021/acsomega.1c01678.

3.Oliveira, E.R.A. et al (2021). Intracellular Sequestration of the NKG2D Ligand MIC B by Species F Adenovirus. Viruses. 13(7):1289. doi: 10.3390/v13071289.

4.Borrás, T. et al. (2020). Generation of a Matrix Gla (Mgp) floxed mouse, followed by conditional knockout, uncovers a new Mgp function in the eye. Sci Rep. 10(1):18583. doi: 10.1038/s41598-020-75031-7.

5.Wei, W.Z. et al. (2020). Diversity Outbred Mice Reveal the Quantitative Trait Locus and Regulatory Cells of HER2 Immunity. J Immunol. doi: 10.4049/jimmunol.2000466.

6.Robles-Rodríguez, O.A. et al. (2020). Antitumor effect of adenoviruses expressing mutant nononcogenic E7 versions from HPV-16 fused to calreticulin. JBUON. 25(1): 543-548.

7.Rollier, C.S. et al. (2020). Modification of Adenovirus vaccine vector-induced immune responses by expression of a signalling molecule. Sci Rep. 10(1):5716. doi: 10.1038/s41598-020-61730-8.

8.Lokhandwala, S. et al. (2019). Adenovirus-vectored African Swine Fever Virus antigen cocktails are immunogenic but not protective against intranasal challenge with Georgia 2007/1 isolate. Veterinary Microbiology. 235:10-20. doi:10.1016/j.vetmic.2019.06.006.

9.Sang, Y. et al. (2019). Ileal transcriptome analysis in obese rats induced by high-fat diets and an adenoviral infection. Int J Obes (Lond). doi: 10.1038/s41366-019-0323-2.

10.Hao, J. et al. (2018). Prostate Cancer-Specific of DD3-driven Oncolytic Virus-harboring mK5 Gene. Open Med (Wars). 14: 1-9. doi: 10.1515/med-2019-0001.

 

③ VPK-106, 腺病毒核酸滴度检测试剂盒

提供了一个快速的方法来测量你的腺病毒的病毒核酸含量。此试验可在病毒纯化之前或之后进行。

 

VPK-106, 腺病毒核酸滴度检测试剂盒

 

发表文章,VPK-106, 腺病毒滴度IHC检测试剂盒:

1.Stone, A.V. et al. (2020). Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus. Cartilage. doi: 10.1177/1947603520958143.

2.Hossain, E. et al. (2020). Advantages of Using Paclitaxel in Combination with Oncolytic Adenovirus Utilizing RNA Destabilization Mechanism. Cancers (Basel). 12(5). pii: E1210. doi: 10.3390/cancers12051210.

3.Habiba, U. et al. (2020). Cisplatin Relocalizes RNA Binding Protein HuR and Enhances the Oncolytic Activity of E4orf6 Deleted Adenovirus. Cancers. 12:809. doi: 10.3390/cancers12040809.

4.Oser, M.G. et al. (2019). The KDM5A/RBP2 histone demethylase represses NOTCH signaling to sustain neuroendocrine differentiation and promote small cell lung cancer tumorigenesis. Genes Dev. doi: 10.1101/gad.328336.119.

5.Yanagawa-Matsuda, A. et al. (2019). Oncolytic potential of an E4-deficient adenovirus that can recognize the stabilization of AU-rich element containing mRNA in cancer cells. Oncol Rep. 41(2):954-960. doi: 10.3892/or.2018.6865.

6.Yan, Y. et al. (2016). HIV-1 Vpr increases HCV replication through VprBP in cell culture. Virus Res. doi:10.1016/j.virusres.2016.07.007.

7.Xie, Y. et al. (2016). A recombinant adenovirus expressing P12A and 3C protein of the type O foot-and-mouth disease virus stimulates systemic and mucosal immune responses in mice. BioMed Res Int. doi:10.1155/2016/7849203.

8.Ran, L. et al. (2016). Delivery of oncolytic adenovirus into the nucleus of tumorigenic cells by umor microparticles for virotherapy. Biomaterials. 89:56-66.

9.Nuche-Berenguer, B. et al. (2016). The p21-activated kinase, PAK2, is important in the activation of numerous pancreatic acinar cell signaling Cascades and in the onset of early pancreatitis events. Biochim Biophys Acta. doi:10.1016/j.bbadis.2016.02.008.

 

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文章来源:【方法大全】Adenovirus Titer,腺病毒滴度快速检测

 

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