2023年12月20日，来自荷兰瓦赫宁根大学Dolf Weijers团队在《细胞》杂志上发表了标题为“RAF-like protein kinases mediate a deeply conserved, rapid auxin response.”的研究成果，研究发现RAF样蛋白激酶介导一种高度保守的快速生长素反应。
据介绍，植物信号分子生长素触发陆地植物和藻类的快速和慢速细胞反应。核生长素途径介导基因表达并控制陆地植物的生长发育，但藻类中没有这种途径。在拟南芥中已经鉴定出几种快速反应的成分，但尚不清楚这些成分是否是保守机制的一部分。
研究人员最近发现了一种对生长素的快速、蛋白质组范围的磷酸化反应。这种反应发生在5种陆地植物和藻类物种上，并集中在一个共同目标的核心群体上。研究人员在同一物种中发现了对生长素的保守快速生理反应，并确定快速加速纤维肉瘤（RAF）样蛋白激酶是生长素触发的跨物种磷酸化的中心介质。遗传分析将这种激酶与生长素触发的蛋白质磷酸化和快速细胞反应联系起来，从而确定了绿色谱系中生长素快速反应的古老机制。
Highlights
• Auxin triggers rapid (<30 s) and specific proteome phosphorylation changes
• Fast auxin-triggered phosphorylation is ancient and widespread in the green lineage
• Phosphorylation-based auxin signaling is mediated by B4 RAF-like kinases
• RAF-like kinases connect fast auxin signaling to cytoplasmic streaming
Summary
The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants and algae. The nuclear auxin pathway mediates gene expression and controls growth and development in land plants, but this pathway is absent from algal sister groups. Several components of rapid responses have been identified in Arabidopsis, but it is unknown if these are part of a conserved mechanism. We recently identified a fast, proteome-wide phosphorylation response to auxin. Here, we show that this response occurs across 5 land plant and algal species and converges on a core group of shared targets. We found conserved rapid physiological responses to auxin in the same species and identified rapidly accelerated fibrosarcoma (RAF)-like protein kinases as central mediators of auxin-triggered phosphorylation across species. Genetic analysis connects this kinase to both auxin-triggered protein phosphorylation and rapid cellular response, thus identifying an ancient mechanism for fast auxin responses in the green lineage.
文章来源：
Andre Kuhn, Mark Roosjen et al, RAF-like protein kinases mediate a deeply conserved, rapid auxin response.DOI: 10.1016/j.cell.2023.11.021,Cell, 最新IF：66.85
