Materials:
Supplies: Procedures: Results:
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- PCR enzyme (5 Units/ul)
- PCR enzyme reaction buffer
- Distilled water (nuclease free)
- DNA to be used as template, such as GAPDH template from Clontech
- DNA to be used as upstream and downstream primers, such as the corresponding primers set for GAPDH template from Clontech
- Light mineral oil (nuclease free)
- dNTP mix (10 mM of each dNTP)
- PCR machine and the 600-ul tubes for use in the machine
- Micropipetter and tips
- Microcentrifuge with adaptors for 600-ul tubes
- dNTP mix (10 mM of each dNTP)
- PCR machine and the 600-ul tubes for use in the machine
- Micropipetter and tips
- Microcentrifuge with adaptors for 600-ul tubes
