FemtoMax 超敏HRP底物，轻松检测飞克级别的抗原

FemtoMax 超灵敏化学发光HRP底物是一种极其灵敏、无放射性、增强型基于鲁米诺化学发光底物，用于检测辣根过氧化物酶（HRP）。FemtoMax 设计用于蛋白质印迹(WB)和酶联免疫吸附测定(ELISA)。FemtoMax 可以使用照相胶片或其他成像方法（包括高灵敏度CCD相机）轻松检测飞克级别的抗原。可以将印迹重复暴露于X射线胶片以获得Z佳结果，或者剥离检测试剂并重新检测。在使用FemtoMax 时可使用与Amersham ECL Plus 底物或Pierce SuperSignal West Femto底物相同的印迹条件。

产品详情：

FemtoMax (货号：FEMTOMAX-020)应用实例展示：

Elisa实验：

不同组的血浆MDS-OAβ的浓度检测：将孵育的血浆样品混合物和连续稀释的标准样品加入各自的孔中，并将板在室温下孵育1小时。然后，加入100µL/孔的增强化学发光底物溶液（p/n Femtomax），并使用Victor 3TM多分光光度计检测相对发光单元（RLU）信号。PMID: 33958861

Korean version of Consortium to Establish a Registry for Alzheimer’s disease（CERAD-K）和血浆低聚β淀粉样蛋白（OAβ）的测试：（a） CERAD-K和OAβ浓度的原始分数。（b）与对照组相比，言语流利度、命名、单词记忆/回忆和总分异常的CERAD组（年龄/性别/教育，调整标准差低于−1.5）的OAβ浓度更高。（c） 高OAβ组（≥0.78 ng/mL）在命名、单词记忆/回忆/识别和总分方面的异常明显更常见。PMID: 32326061

Western Blot实验：

CK2.3对RANKL诱导的破骨细胞生成中p-Erk1/2的影响：CK2.3在RAW264.7细胞中刺激24小时后增加p-Erk 1/2，如（A）蛋白质印迹所测定的。将印迹在3%BSA中孵育1小时以封闭非特异性结合。抗体以1:1000稀释液（在1%BSA中）在4°C下过夜使用。随后与HRP抗兔二抗以1:5000稀释液（在1%BSA中）孵育。印迹在化学发光FemtoMax超敏HRP底物（p/n FemtoMax）中孵育2分钟。PMID:3260129

使用Femtomax的小鼠抗AKT pS473抗体的蛋白质印迹：泳道1：未处理细胞中的非磷酸化AKT。泳道2：PDGF刺激的NIH/3T3细胞裂解物上磷酸化的AKT（用箭头指示在~56kDa处）。上样量：每泳道10µg。一抗：AKT pS473抗体，1:10000，TBS，含0.05% Tween-20和1%BSA，在4°C下1小时。二抗：HRP标记的Gt-a-Mouse IgG（p/n 610-103-121），以1:20000稀释，在4℃下与化学发光FemtoMax 超敏HRP底物（p/n FemtoMax-100）一起使用1小时。批号：25245AB

抗GST标签抗体的蛋白质印迹：泳道1：重组GST标记的重组蛋白5μg；泳道2：重组GST-标记的重组蛋白质2μg；泳道3:重组GST标记的重组蛋白1ug。泳道4:重组GST标记的重组蛋白500 ng。泳道5:重组GST标记的重组蛋白250 ng。泳道6:重组GST标记的重组蛋白100 ng。泳道7:重组GST标记的重组蛋白50 ng。一抗:抗gst抗体，1:1000，在4℃下过夜。二抗：驴二抗，1:10000，室温45分钟。封闭：5%BLOTTO，4°C过夜。预测/观测大小：78 kDa。

使用化学发光FemtoMax HRP底物进行蛋白质印迹检测：rPARP1结构域在4°C下使用一抗兔血清1:500过夜曝光3秒后在11 kDa处检测到。二抗HRP山羊抗兔IgG抗体1:40000。所有孵育均在用于荧光蛋白封闭的封闭缓冲液（p/n MB‐070）中进行。

化学发光FemtoMax 超灵敏HRP底物和竞争对手的比较：GAPDH蛋白以1000、250、62.5、15.6ngs的上样量点在硝化纤维膜上。将膜在室温下封闭一小时。一抗：Rb抗GAPDH1:2000稀释，在4°C下孵育过夜。洗涤后，二抗：Gt抗Rb IgG HRP在室温下以1:50000稀释孵育2小时。检测：化学发光FemtoMax 超敏HRP底物Vs竞争对手1-4。

FemtoMax (货号：FEMTOMAX-020)引文展示：

1. Mollin M et al.(2021). Clinical, functional and genetic characterization of 16 patients suffering from chronic granulomatous disease variants – identification of 11 novel mutations in CYBB. Clin Exp Immunol.
2. Bakri FG et al.(2021). Second Report of Chronic Granulomatous Disease in Jordan: Clinical and Genetic Description of 31 Patients From 21 Different Families, Including Families From Lybia and Iraq. Front Immunol.
3. Pyun JM et al.(2021). Plasma Amyloid-β Oligomerization Tendency Predicts Amyloid PET Positivity. Clinical Interventions in Aging
4. Kim MK et al.(2021). A novel GPR119 agonist DA-1241 preserves pancreatic function via the suppression of ER stress and increased PDX1 expression. Biomed Pharmacother.
5. Zhang L et al.(2020). Functional analysis of miR-767-5p during the progression of hepatocellular carcinoma and the clinical relevance of its dysregulation. Histochem Cell Biol.
6. Nguyen J et al.(2020). A Synthetic Peptide, CK2. 3, Inhibits RANKL-Induced Osteoclastogenesis through BMPRIa and ERK Signaling Pathway. J Dev Biol.
7. Durbano HW et al.(2020). Aberrant BMP2 Signaling in Patients Diagnosed with Osteoporosis. Int J Mol Sci.
8. Youn YC. et al.(2020). Blood Amyloid-β Oligomerization as a Biomarker of Alzheimer's Disease: A Blinded Validation Study. J Alzheimers Dis.
9. Brault J. et al.(2020). NOX4 is the main NADPH oxidase involved in the early stages of hematopoietic differentiation from human induced pluripotent stem cells. Free Radic Biol Med.
10. Lee JJ et al.(2020). Association of Plasma Oligomerized Beta Amyloid with Neurocognitive Battery Using Korean Version of Consortium to Establish a Registry for Alzheimer's Disease in Health Screening Population. Diagnostics (Basel, Switzerland)
11. Grafen A et al.(2019). Use of acid ceramidase and sphingosine kinase inhibitors as antiviral compounds against measles virus infection of lymphocytes in vitro. Front Cell Dev Biol
12. John V et al.(2019). Caveolin-1 controls vesicular TLR2 expression, p38 signaling and T cell suppression in BCG infected murine monocytic myeloid-derived suppressor cells. Front Immunol.
13. Chung et al.(2018). Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development
14. Kim TH et al.(2018). Additive effects of evogliptin in combination with pioglitazone on fasting glucose control through direct and indirect hepatic effects in diabetic mice. Eur J Pharmacol.
15. Tiwarekar V et al.(2018). APOBEC3G-regulated host factors interfere with measles virus replication: role of REDD1 and mammalian TORC1 inhibition. J Virol.
16. Dietrich K et al.(2018). Health-Relevant Phenotypes in the Offspring of Mice Given CAR Activators Prior to Pregnancy. Drug Metab Dispos.
17. Barfeld SJ et al.(2017). c-Myc antagonises the transcriptional activity of the androgen receptor in prostate cancer affecting key gene networks.
18. An SSA et al.(2017). Dynamic changes of oligomeric amyloid β levels in plasma induced by spiked synthetic Aβ42. Alzheimer's Research & Therapy
19. Wang MJ et al.(2017). Oligomeric forms of amyloid-β protein in plasma as a potential blood-based biomarker for Alzheimer's disease. Alzheimer's Research & Therapy
20. Tadokoro T et al.(2016). BMP signaling and cellular dynamics during regeneration of airway epithelium from basal progenitors.
21. Wache C et al.(2015). Myeloid-related protein 14 promotes inflammation and injury in meningitis. J Infect Dis.
22. Carter LG et al.(2015). Exercise improves glucose disposal and insulin signaling in pregnant mice fed a high fat diet. J Diabetes Metab.
23. Beaumel S et al.(2014). Identification of NOX2 regions for normal biosynthesis of cytochrome b558 in phagocytes highlighting essential residues for p22phox binding. Biochem J.
24. Hohne C et al.(2013). High mobility group box 1 prolongs inflammation and worsens disease in pneumococcal meningitis.
25. Huang BW et al.(2013). Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. FASEB J.