请教质谱高人啊,这结果怎么分析啊

我的蛋白样品到某公司去作简单质谱分析,利用的叫maldi-tof技术(激光解吸电离-飞行时间),可以分析样品的肽指纹图谱,他们还通过源后衰变(post souce decay,PSD)来获得一级结构数据。

我得到了结果,但我实在不太懂怎么分析。看了本站的一些讨论,还是有点稀里糊涂。因为我的样品是个未知蛋白,所以我不知道哪个匹配结果可信,可信度也没什么概念。像下面这个,55的得分,27%的覆盖相似性,说明得了多少问题呢?(附件有我的结果,可信件与我联系,3183229@sina.com) Match to: P12217 Score: 55 Expect: 0.046 (ACCD_MARPO) Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (EC 6.4.1.2) (ACCase beta chain) Acetyl-coenzyme A carboxylase carboxyl transferas Nominal mass (Mr): 35803; Calculated pI value: 8.77 NCBI BLAST search of P12217 against nr Unformatted sequence string for pasting into other applications Taxonomy: Marchantia polymorpha Variable modifications: Oxidation (M),Pyro-glu (N-term E) Cleavage by Trypsin: cuts C-term side of KR unless next residue is P Number of mass values searched: 46 Number of mass values matched: 10 Sequence Coverage: 27% Matched peptides shown in Bold Red1 MSLMNWFEDK RRFGGLIGAF IEKATKGYIF SEREKDRYIK IDTTKGLWTR51 CDNCENMLYV RFLRQNKRIC EECGYHLQMS STERIELLID RGTWYPMDED101 MTARDVLKFS DEDSYKNRIA FYQKRTGLTD AIQTGIGQLN GIPIALGVMD151 FQFMGGSMGS VVGEKITRLI EYATRASMPL IIVCSSGGAR MQEGTLSLMQ201 MAKISSVLQI HQAQKRLLYI AILTYPTTGG VTASFGMLGD IIIAEPKAYI251 AFAGKRVIEQ TLRQKIPDGF QVAESLFDHG LLDLIVPRNL LKGVLSEIFE301 LYNAAPCKKF QNSFFK

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