Wnt signaling plays several important roles in the development of the zebrafish central nervous system (CNS). This chapter outlines both the known and postulated roles of Wnts from the earliest step of neural plate induction to relatively late events such as axon pathfinding and synaptogenesis. The common tools useful for examining Wnt function and nervous system development in zebrafish are first reviewed. Examples are then provided for specific phenotypes resulting from gain and loss of Wnt activity at multiple developmental stages. Finally, specific assays and reagents that can be used to investigate the function of novel Wnt pathway components in CNS development are listed.
