The study of mitochondrial division proteins has largely focused on yeast and mammalian cells. We describe methods to useCaenorhabditis elegansas an alternative model for studying mitochondrial division, taking advantage of the many wonderful resources provided by theC. eleganscommunity. Our methods are largely based on manipulation of gene expression using classic and molecular genetic techniques combined with fluorescence microscopy. Some biochemical methods are also included. As antibodies become available, these biochemical methods are likely to become more sophisticated.
