A real-time kinetic tool for polymerase chain reaction (PCR) quantitation, AmpliSensor assay (1 ) quantifies PCR product by relating the rate of an amplification reaction through the progressive depletion of a rate-limiting primer. AmpliSensor assay invokes a two-step amplification scheme:
| 1. | An initial amplification with one primer in excess in order to overproduce one strand of the target, and |
| 2. | A subsequent hemi-nested amplification for fluorescence signal detection. |
