This chapter describes the technology and selected applications in the field of protein analysis for free flow electrophorsis (FFE). FFE is a highly versatile technology for applications in this field as a result of its continuous processing of sample and the possibility of separating almost any kind of charged or chargeable particles in an aqueous medium without any solid matrix such as acrylamide. Possible applications are separation of ions, proteins, peptides, organelles, or whole cells for subsequent analysis.
