Reagents / Solutions
Lysis Buffer:
10ml 10% Sodium dodecyl sulphate (SDS)
10ml Glycerol
10ml b-mercaptoethanol
8ml 0.5M Tris pH6.8
1ml 0.1% bromophenol blue
51ml H2O
Protocol
Spin down 106 cells and wash, if required, in phosphate buffered saline.
Resuspend cells in 100µl warmed lysis buffer, vortex then boil for 5 - 10 minutes to break up DNA.
Cool (not on ice - the SDS will precipitate) and centrifuge to collect droplets.
Vortex briefly to mix.
The samples may now be analysed by , or
Store samples at -20℃ until required.
