macerate tissue in Eppendorf tube without butter at RTadd 400 m l extraction buffe...
生物知识
生物知识
生物知识
Electro-elution of nucleic acids from agarose and polyacrylamide gel slides
PrincipleThe nucleic acids contained within the gel slice are electrophoresed out ...
生物知识
Alkaline Southern Blotting Procedure
Author: Suzanne GerttulaDate: March 14,1994Digest between 1 and 10 ug Genomic DNA....
生物知识
PEG Preparation of Plasmid DNA
PEG Preparation of PlasmidPlasmid isolated by this procedure can be used routinely...
生物知识
DNA Plasmid Prep.
This is a scaled up version of the Baron protocol which has been modified to achie...
生物知识
S1 Nuclease Protection Assay
End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci/m...
生物知识
Histochemical detection重组质体的检定
操作原理与colony hybridization 相同，但是将滤膜移置于含有IPTG 及5-bromo-4-chloro-3-indolyl β-D-glucur...
生物知识
Increasing Sensitivity in Northern Analysis with RNA Probes
Note: The pointers in this technical bulletin presuppose the use of standard 50% f...
生物知识
Genome Walking Kit 使用注意
用于Genome Walking Kit的模板总DNA 一般要满足以下几点：1、DNA 的完整性，电泳为单一条带。2、避免杂质，OD260/280=1.8~2.0。...
