生物知识 Extraction of DNA from Schizophyllum commune (裂褶菌DNA提取) 1. Transfer plugs of mycelial margin to 0.1 P plates. Allow to grow to fill plate ... 05月12日 发表评论 阅读全文
生物知识 DNA的琼脂糖纯化(John Roth, Division of Biological Sciences,U 1.Separate DNA fragments in an agarose gel cast with 0.5 mg/ml Ethidium bromide.Lo... 05月12日 发表评论 阅读全文
生物知识 Plasmid Mini-prep Protocol 1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high... 05月12日 发表评论 阅读全文
生物知识 PREPARATION OF PLASMID DNA FOR SEQUENCING The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S... 05月12日 发表评论 阅读全文
生物知识 Analys of Genomic DNA by Southern Hybridization Localization of particular sequences within genomic DNA is usually accomplished by... 05月12日 发表评论 阅读全文
生物知识 DNA Purification-Modified CTAB Procedure This protocol originated in Murray and Thompson (1980) and then was modified by Ri... 05月12日 发表评论 阅读全文
生物知识 DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS This is a four day procedure so it's best to start on Monday or Tuesday.CASE ... 05月12日 发表评论 阅读全文
生物知识 DNA Preparation from Fresh/Frozen Tissue ReagentsChloroform EDTA,0.5 M Ethanol, absolute Isoamyl alcohol Sigma,Cat.I-3643 P... 05月12日 发表评论 阅读全文
生物知识 Genomic DNA Extraction for Mapping 1.grow plants in trays of 96 and leave two spots open (for the PCR controls)2.harv... 05月12日 发表评论 阅读全文
生物知识 BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method 1、A smear (rather than a single colony) of BAC colonies are picked and transferred... 05月12日 发表评论 阅读全文