This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1.0...
生物知识
生物知识
生物知识
silica回收DNA片段
1、在长波紫外灯下切下含有目标DNA的琼脂糖凝胶， 用纸巾吸干凝胶表面的液体并切小。计算凝胶的重量。2、加入2-3倍体积的6M KI或NaI（避光4度保存）。3、温...
生物知识
Electroelution of DNA from Agarose
Electroelution of agarose fragmentsElectroelution buffer1 M Tris, pH 7.5 12.0 mls0...
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Membrane Transfer and Crosslinking for RNA
Analysis of mRNA expression in tissue or cell culture is often done by Northern bl...
生物知识
Mag-Bind RX Plasmid Isolation Protocol
一、实验试剂 Absolute (96%-100%) ethanol 二、实验设备 1. Centrifuge with swinging-bucket rotor...
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Extraction of genomic DNA from whole blood
Abstract: The protocol is simple and fairly rapid.It does not require the use of o...
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DNA Extraction from Cheek Cells
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of any ...
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Single Strand DNA Prep. for Sequencing
This protocol works well from a 5 ml starting culture or a 1 ml starting culture (...
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Cetyltrimethylammoniumbromide(CTAB)Pla
Procedure1. Grind 2 to 5 g of frozen leaves to a very fine powder with a liquid ni...
生物知识
DNA isolation
CTAB TECHNIQUE / Method / Schedule / Protocol FOR DNA ISOLATION / DNA EXTRACTION F...
