组织蛋白酶B (Cathepsin B, CTSB)是一种广泛表达于溶酶体上的半胱氨酸肽链内切酶，属于木瓜蛋白酶家族的成员，广泛存在于从病毒到哺乳动物和人等生物体内。可通过参与细胞凋亡、细胞外基质降解、抗原呈递、炎症反应、细胞自噬等多种病理生理过程从而参与多种疾病的发生和发展。
市面上常见的是检测细胞裂解液中的组织蛋白酶B活性，更多研究者希望动态检测 活细胞 内的组织蛋白酶B的活性，做更真实的实验！艾美捷科技为您推荐Immunochemistry Tech（ICT）品牌专利Magic Red系列产品。
ICT的Magic Red®检测法使研究人员能够在体外对细胞内的蛋白酶活性进行定量和监测。
| 货号 | 名称 | 规格 | 样本 |
| ICT-937 | Magic Red® Cathepsin-B Assay Kit
魔力红®组织蛋白酶B活性分析试剂盒 |
25T | 培养的活细胞
(悬浮和贴壁) |
| ICT-938 | 100T |
* 仅供科研使用，不得用于医疗.
【原理】
Magic Red®魔力红底物，是细胞渗透性的，它很容易穿透细胞膜和内部细胞器的膜——不需要裂解或渗透步骤。如果组织蛋白酶是活性的，魔红底物被裂解，甲酰紫荧光团将在激发时变为荧光（Ex/Em =592nm/628nm）。随着酶活性的进展和更多魔红底物的裂解，信号将增强，使研究人员能够观察颜色随时间的变化。样品可通过荧光显微镜或荧光酶标仪进行分析。试剂盒中包括Hoechst 33342和吖啶橙，分别用于检测核形态和溶酶体细胞器结构。
【产品组分】
| Magic Red® Cathepsin-B Assay Kit 魔力红®组织蛋白酶B活性分析试剂盒 |
|
| 25T | 100T |
| l Magic Red Substrate (MR-RR2), 1 25 Test Vial, #6133 l Hoechst 33342, 1 mL, #639 l Acridine Orange, 0.5 mL, #6130 l Kit Manual |
l Magic Red Substrate (MR-RR2), 1 100 Test Vial, #6134 l Hoechst 33342, 1 mL, #639 l Acridine Orange, 0.5 mL, #6130 l Kit Manual |
| 保存条件：2-8°C 避光保存。 | |
| 点击下载说明书PDF | |
【Nature文章鉴赏】
IF=28.2, 《Lysosomal retargeting of Myoferlin mitigates membrane stress to enable pancreatic cancer growth》,Nature Cell Biology
Magic Red®相关产品列表：
| 货号 | 品牌 | 名称 | 规格（T） |
| ICT-935 | Immunochemistry | Magic Red™ Caspase 3 & 7 Assay Kit | 25 |
| ICT-936 | Immunochemistry | Magic Red™ Caspase 3 & 7 Assay Kit | 100 |
| ICT-937 | Immunochemistry | Magic Red™ Cathepsin B Assay Kit | 25 |
| ICT-938 | Immunochemistry | Magic Red™ Cathepsin B Assay Kit | 100 |
| ICT-939 | Immunochemistry | Magic Red™ Cathepsin K Assay Kit | 25 |
| ICT-940 | Immunochemistry | Magic Red™ Cathepsin K Assay Kit | 100 |
| ICT-941 | Immunochemistry | Magic Red™ Cathepsin L Assay Kit | 25 |
| ICT-942 | Immunochemistry | Magic Red™ Cathepsin L Assay Kit | 100 |
【Magic Red® Cathepsin-B Assay Kit，更多发表文章，28+】
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10、Zhong, W;Rao, Z;Xu, J;Sun, Y;Hu, H;Wang, P;Xia, Y;Pan, X;Tang, W;Chen, Z;Zhou, H;Wang, X. Defective mitophagy in aged macrophages promotes mitochondrial DNA cytosolic leakage to activate STING signaling during liver sterile inflammation. Aging Cell. 2022 May 22; doi: 10.1111/jcpe.13647. Abstract
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14、Yin Q, Jian Y, Xu M, Huang X, Wang N, Liu Z, Li Q, Li J, Zhou H, Xu L, Wang Y, Yang C. CDK4/6 regulate lysosome biogenesis through TFEB/TFE3. J Cell Biol. 2020 Aug 3;219(8):e201911036. doi: 10.1083/jcb.201911036. Full Text
15、Almacellas E, Pelletier J, Day C, Ambrosio S, Tauler A, Mauvezin C. Lysosomal degradation ensures accurate chromosomal segregation to prevent chromosomal instability. Autophagy. 2020 Jun 23;1-18. doi: 10.1080/15548627.2020.1764727. Online ahead of print. Full Text
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美国ICT除了可提供全面的细胞活性检测相关试剂，包括凋亡、 caspase、 自噬、细胞毒性、坏死等相关检测，还可以同时提供高质量高灵敏度的 ELISA以及相关试剂，包括包被液、封闭液、 冲洗液、 底物、 终止液、 板子等等。
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来源：ImmunoChemistry Magic Red 轻松检测细胞内组织蛋白酶B（Cathepsin B）活力
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