原理限制性片段长度多态性 PCR（PCR-RFLP），首先利用 PCR 扩增目的基因，然后用限制性内切酶酶解样品 DNA，产生大量的限制性酶切片段，通过凝胶电泳分离...
生物知识
生物知识
生物知识
肝炎病毒的标本采集和处理方法
简介病原学来源不同的肝炎其实验室检测差异较大，病原学诊断主要涉及甲、乙、丙、丁、戊五型，包括各种肝炎病毒的核酸定性及定量，抗原组分及抗体等三个层次的检测，检查的目的...
生物知识
反渗透和超过滤法
过滤方法用孔径较大的半透膜，在压差（一般在0.7～10千克力/厘米2）和紊流流动的情况下，溶液中的溶剂和电解质等能透过半渗透膜，而胶体、微粒和分子量较大的物质则被截...
生物知识
Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
We describe an alternative to the molecular biology technique of polyacrylamide ge...
生物知识
An antibody-lectin sandwich assay for quantifying protein glycoforms
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of pr...
生物知识
An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri
We describe an improved, efficient and reliable method for the vapour-phase silani...
生物知识
Purification and Proteomic Analysis of a Nuclear-Insoluble Protein Fraction
We describe here a method for analyzing a rat liver nuclear-insoluble protein frac...
生物知识
Biosensors Based on Carbon Nanotube-Network Field-Effect Transistors
We describe in detail the different steps involved in the construction of a carbon...
生物知识
Force and compliance measurements on living cells using atomic force microscopy (AFM)
We describe the use of atomic force microscopy (AFM) in studies of cell adhesion a...
生物知识
Fabrication of Polydimethylsiloxane Microfluidics Using SU-8 Molds
We detail the widely prevalent technique of polydimethylsiloxane (PDMS) molding us...
