生物知识 Use of Bimolecular Fluorescence Complementation in Yeast Saccharomyces cerevisiae Visualization of protein–protein interactionsin vivooffers a powerful tool to reso... 04月16日 发表评论 阅读全文
生物知识 Negative staining and image classification powerful tools in modern electron microscopy Vitrification is the state-of-the-art specimen preparation technique for molecular... 04月16日 发表评论 阅读全文
生物知识 Whole-Cell Patch-Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterol Voltage-sensitive calcium channels (VSCC) are vital to the normal physiology of ma... 04月16日 发表评论 阅读全文
生物知识 In Vitro O-Antigen Ligase Assay WaaL is a membrane enzyme that catalyzes the glycosidic bonding of a sugar at the ... 04月16日 发表评论 阅读全文
生物知识 Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling We demonstrate the quantification capability and robustness of a new integrated li... 04月16日 发表评论 阅读全文
生物知识 A Nonradioactive Assay for Nitric Oxide Synthase Activity in Tissue Extracts We describe a fluorescence assay for nitric oxide synthase activity based on a new... 04月16日 发表评论 阅读全文
生物知识 Protein Processing Characterized by a Gel-Free Proteomics Approach We describe a method for the specific isolation of representative N-terminal pepti... 04月16日 发表评论 阅读全文
生物知识 Quantitative Proteomics of Mouse Brain and Specific Protein-Interaction Studies Using Stable Isotope We describe a new method for quantitative tissue proteomics using culture-derived ... 04月16日 发表评论 阅读全文
生物知识 Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the sa We describe a semiquantitative RT-PCR protocol optimized in our laboratory to extr... 04月16日 发表评论 阅读全文
生物知识 Systems for the Cell-Free Synthesis of Proteins We describe a system for the cell-free expression of proteins based on extracts fr... 04月16日 发表评论 阅读全文