简介DNA 体外重组，又称基因克隆，是基因操作中最基本的技术，也是分子生物学的核心技术。DNA 体外重组包括：欲进行重组的 DNA 片段（常称为目的 DNA) 的获...
生物知识
生物知识
生物知识
Detection of ERK1/2 Activities Using Affinity Reagents
The three major mammalian mitogen-activated protein kinase (MAPK) subfamilies incl...
生物知识
Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
The time and labor required for generating nucleotide sequence information has bee...
生物知识
Resin Technologies: Construction and Staining of Resin TMAs
The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the tis...
生物知识
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
The traditional method for determining the transcription rate of a gene, nuclear r...
生物知识
Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
The transfer or “transfectron” of genetic material into tissue-culture cell lines ...
生物知识
Inserting and Manipulating DNA in a Nanopore with Optical Tweezers
The translocation of small molecules and polymers is an integral process for the f...
生物知识
Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
The transport of membrane-bound proteins through post-Golgi compartments depends o...
生物知识
Yeast Two-Hybrid Library Screening
The two-hybrid system was originally devised by Fields and Song as a protein inter...
生物知识
A method to identify p62s UBA domain interacting proteins
The UBA domain is a conserved sequence motif among polyubiquitin binding proteins....
