The technology of exon trapping, sometimes called exon amplification, strives to e...
生物知识
生物知识
生物知识
Scale-Up of Natural Products Isolation
The term “scale-up” has been interpreted here as increasing the output of the prod...
生物知识
Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Control
The term eicosanoid is derived from the chemical name for arachidonic acid, eicosa...
生物知识
The Isolation of Minicells
The term minicell was introduced by Adler et al. (1 ) for the small spherical cell...
生物知识
On-Line Preconcentration and Separation of Antisense Oligonucleotides by ITP-CE in Dextran-Filled Ca
The therapeutic use of “antisense oligonucleotides” is a new approach to rational ...
生物知识
Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biologic
The thiobarbituric acid (TBA) assay was developed to quantitatively determine lipi...
生物知识
细胞培养中遇到细胞空泡化怎么办？
细胞培养中遇到细胞空泡化怎么办？1、细胞老化：细胞处于终末分化状态，或细胞传代次数过多，老化严重。2、pH值：培养液的pH值与细胞正常所需pH值差别太大。3、胰酶消...
生物知识
病毒的预防与治疗
简介病毒的预防与治疗根据病毒种类不同而不同，应对症下药。材料与仪器器材：①一次性无菌手套②标本采集管③棉签拭子④PCR仪试剂：①材料：待采标本②PBS缓冲液③琼脂步...
生物知识
用随机寡核苷酸引物从mRNA合成cDNA探针
原理本方案以 poly(A)+RNA 作为模板，通过随机引物反应合成 cDNA 探针，这类探针可用于 cDNA 文库的差异筛选。材料与仪器反转录酶 反转录酶缓冲液 ...
生物知识
虫媒病毒的血清学检测
简介虫媒病毒（ arbovirus）是指一些通过吸血节肢动物叮咬敏感脊椎动物而引起的自然疫源性疾病及人兽共患病的一群病毒，包括不同基因组的许多病毒家族，如黄病毒科（...
