生物知识 The Teratoma Assay: An In Vivo Assessment of Pluripotency A teratoma is a nonmalignant tumor comprised of a disorganized mixture of cells an... 05月04日 发表评论 阅读全文
生物知识 Retroviral Transduction of FACS-Purified Hematopoietic Stem Cells Since the mid-1980s, murine retroviral vectors have been used extensively by a num... 05月04日 发表评论 阅读全文
生物知识 Long-Term Cryopreservation of Thylakoid Membranes Chloroplast thylakoids are the site of photosynthetic light reactions, electron tr... 05月04日 发表评论 阅读全文
生物知识 A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY MATERIALS :1. 1 X PBS (PBSAz, 1 X PBS, e.g., Irvine Scientific, CA, containing 2% ... 05月04日 发表评论 阅读全文
生物知识 Cytometric Detection and Quantitation of Cell-Cell Electrofusion Products Cell-cell electrofusion (CCE) is a process that involves forcing cells into close ... 05月04日 发表评论 阅读全文
生物知识 Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protei The insulin-like growth factor-binding proteins (IGFBPs) are carrier proteins that... 05月04日 发表评论 阅读全文
生物知识 Knock-Down of Gene Expression in Hematopoietic Cells RNA interference (RNAi) is an evolutionarily conserved sequence-specific post-tran... 05月04日 发表评论 阅读全文
生物知识 Phylogenetic Inference and Parsimony Analysis Application of phylogenetic inference methods to comparative endocrinology studies... 05月03日 发表评论 阅读全文
生物知识 RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium Knockouts by homologous recombination are frequently used to investigate the funct... 05月03日 发表评论 阅读全文
生物知识 限制酶和DNA 甲基化酶 限制酶特异性地结合于一段被称为限制酶识别序列的特殊DNA 序列之内或其附近的特异位点上,井在此切割双链DNA 。它可分3 类,分别是第一型(Type I)、第二型(... 05月03日 发表评论 阅读全文