前列腺素E2受体研究工具:分分钟提升你逼格

摘要

想研究前列腺素E2受体(EP),没有研究EP体系的工具怎么行!艾美捷为您整理准备了全面的EP研究体系的工具,分分钟提升你逼格的EP研究工具,什么工具那么神?快跟着艾美捷来看看吧!

前列腺素E2(Prostaglandin E2 Receptor, PGE2)是花生四烯酸在很多细胞中代谢的主要产物。跟很多类花生酸一样,PGE2不会预先存在于细胞中。当细胞活化或是存在外源的花生四烯酸时,PGE2合成并释放到胞外。前列腺素E2受体(EP)是一类G蛋白偶联受体,PGE2的作用依赖于细胞上表达的具有不同功能的PGE2受体EP亚型的激活。

研究发现EP在机体几乎所有系统,如心血管系统、呼吸系统、生殖系统、神经系统等等,在多种疾病的发生中发挥作用。在新年来临之际,艾美捷为您整理准备了全面的EP研究体系的工具。从抗体、抑制剂到活性分析试剂盒,满足您的一切需求。

 

EP抗体汇总

(J CLIN INVEST、Cancer Res等杂志有过引用哟~)

名称 货号 适应性 详情
Anti-Prostanoid EP1 Receptor (extracellular) APR-063 IC, LCI, WB(IFC, IH, IP) 详情
Anti-Prostanoid EP2 Receptor (extracellular) APR-064 IFC, IH, WB(IC, IP, LCI) 详情
Anti-Prostanoid EP3 Receptor APR-065 WB(IC, IFC, IH, IP) 详情
EP1 Receptor Polyclonal Antibody 101740 ICC and WB 详情
EP2 Receptor Polyclonal PE Antibody 10477 FC、ICC 详情
EP2 Receptor Polyclonal Antibody 101750 ICC 、WB 详情
EP3 Receptor Polyclonal Antibody 101760 ICC and WB 详情
EP4 Receptor (C-Term) Polyclonal Antibody 101775 ICC, IHC, and WB 详情
EP4 Receptor (N-Term) Polyclonal Antiserum 101770 WB 详情
EP4 Receptor (C-Term) Polyclonal PE Antibody 10479 FC、IF 详情
Anti-EP4 Receptor (C-Term):SureLight® APC 16625 FC ,cell-based assays 详情
Prostaglandin E Synthase (cytosolic) Monoclonal Antibody 18219 WB,IP,ELISA 详情

 

EP1 Receptor

Expression of Prostanoid EP1 Receptor in human U-87 MG cells

Immunocytochemical staining of live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells with Anti-Prostanoid EP1 Receptor (extracellular) antibody (#APR-063), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.

wb ep2

Western blot analysis of rat brain lysate (lanes 1 and 4), rat stomach  lysate (lanes 2 and 5) and mouse kidney lysate (lanes 3 and 6):

1-3. Anti-Prostanoid EP2 Receptor (extracellular) antibody (#APR-064), (1:500).

4-6. Anti-Prostanoid EP2 Receptor (extracellular) antibody, preincubated with the control peptide antigen.

ep2 exp

Expression of Prostanoid EP2 Receptor in rat cerebellum

Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostanoid EP2 Receptor (extracellular) antibody (#APR-064), (1:100). Prostanoid EP2 Receptor staining (red) is detected inPurkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.

 

EP活性检测试剂盒

(检测灵敏度高,重复性好,Cancer Res等杂志有过引用哟~)

名称 货号 适应性 详情
EP2 Receptor (rat) Reporter Assay Kit 600340 rat EP2 receptor 详情
EP4 Receptor (rat) Reporter Assay Kit 600350 rat EP4 receptor 详情
EP4 Receptor (rat) Activation Assay Kit (cAMP) 600410 EP4 receptor agonists screening 详情

 

PGE2 seap activity

 

EP拮抗剂

名称 货号 适应性 剂量 详情
SC-51322 10010744 EP1 antagonist ED50 = 0.9 mg/kg (mouse) 详情
SC-51089 10011561 EP1 antagonist ED50 = 6.3 mg/kg(mice) 详情
AH 6809 14050 EP and DP receptor EC50 of about 5 x 105 M
Misoprostol 13821 EP2, EP3 and EP4 antagonist EC50 = 0.35 µM(human neutrophils) 详情

 

好了,EP研究工具就暂时整理到这里,另附上PGE2的ELISA试剂盒相关介绍(PGE2 ELISA),拿走不谢哟~

PGE2 tel

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