生物知识 Analysis of Microtubule Assembly Kinetics Using Turbidimetry Turbidity measurements are rapid and reliable methods to follow the effects of dru... 05月03日 发表评论 阅读全文
生物知识 Expression of Recombinant ADAMTS in Insect Cells The “a disintegrin and metalloproteinase with thrombospondin motifs” (ADAMTS) enzy... 05月03日 发表评论 阅读全文
生物知识 Isolation and Culture of Adult Mouse Hepatocytes The liver performs a multitude of functions including the regulation of carbohydra... 05月03日 发表评论 阅读全文
生物知识 Application of Cre/loxP in Drosophila: Site-Specific Recombination and Transgene Coplacement The use of site-specific recombinases has revolutionized the genetic analysis of d... 05月03日 发表评论 阅读全文
生物知识 Transplantation of Human Embryonic Stem Cells and Derivatives to the Chick Embryo Traditional methods of studying the differentiation of human embryonic stem cells ... 05月03日 发表评论 阅读全文
生物知识 Isolation, Characterization, and Reaggregate Culture of Thymic Epithelial Cells The thymus organ is composed of a three-dimensional (3D) network of adjoining epit... 05月03日 发表评论 阅读全文
生物知识 Isolation of mRNAs Encoding Peroxisomal Proteins from Yeast Using a Combined Cell Fractionation and Targeted mRNA localization to distinct subcellular sites occurs throughout the euk... 05月03日 发表评论 阅读全文
生物知识 Analysing the ATP Turnover Cycle of Microtubule Motors Proteins of the kinesin superfamily share a conserved motor domain, which both hyd... 05月03日 发表评论 阅读全文
生物知识 Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of Inhibition of DNA replication in eukaryotic cells was one of the earliest effects ... 05月03日 发表评论 阅读全文
生物知识 Analysis of EGF Receptor Interactions With the Subunit of the Stimulatory GTP Binding Protein of Ade Besides stimulating the mitogen-activated protein kinase, phospholipase Cγ, and ph... 05月03日 发表评论 阅读全文