生物知识 In Vitro Assays for Neural Stem Cell Differentiation Neural stem cells (NSCs) are an undifferentiated population of cells residing in t... 05月03日 发表评论 阅读全文
生物知识 Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells The major limitations in gene transduction to embryonic stem (ES) cells are (1) lo... 05月03日 发表评论 阅读全文
生物知识 体外肝细胞损伤模型建立(CCl4和H2O2) 1 大鼠肝细胞的分离和培养 大鼠4%戊巴比妥麻醉,门静脉插管,先以无钙灌流液灌流,继以37℃通入O2的Ⅳ型胶原酶灌流液继续循环灌流15 min。将肝脏移至一平皿内,... 05月03日 发表评论 阅读全文
生物知识 Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress The upregulation of stress proteins is an important step in the cellular defense a... 05月03日 发表评论 阅读全文
生物知识 Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell T Monitoring of minimal residual disease (MRD) in patients with acute or chronic mye... 05月03日 发表评论 阅读全文
生物知识 The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Tight junctions (TJs) are intercellular structures in epithelial and endothelial c... 05月03日 发表评论 阅读全文
生物知识 培养物的污染及防止 相关专题 按现代的观念,凡是混入培养环境中对细胞生存产生有害的成分和造成细胞不纯的异物都应该视为污染。根据这一概念,组织培养污染物应包括生物(真菌、细菌 、病毒和支... 05月03日 发表评论 阅读全文
生物知识 Transspecies Grafting as a Tool to Understand the Basis of Murine Developmental Limb Abnormalities Elongation along the proximal distal axis of the limb, from either the pectoral or... 05月03日 发表评论 阅读全文
生物知识 Direct Sequencing with Highly Degenerate and lnosine-Containing Primers Among the many techniques of cloning new genes, one approach involves degenerate p... 05月03日 发表评论 阅读全文
生物知识 Hematopoietic Progenitor Cell Collection Hematopoietic progenitor cells can be mobilized from the bone marrow microenvironm... 05月03日 发表评论 阅读全文