大多数蛋白质凝胶电泳是还原性条件下的十二烧基硫酸钠聚丙烯酰胺凝胶电泳(SDS- PAGE) 。＊样品准备还原试剂2－巯基乙醇或者二硫苏糖醇加在变性胶的样品缓冲液中，...
生物知识
生物知识
生物知识
如何做好western-blot实验
1.样品质量。所抽取样品的蛋白含量，蛋白变性是否充分等等，还有，蛋白样品的PH值是否在7~8之间。这会直接影响到样品浓缩的效果。此外，蛋白样品是否降解、目的蛋白是否...
生物知识
western-blot实验中ECL发光检测操作步骤
ECL Western特异发光检测试剂盒用于辣根过氧化物酶(HRP)标记的蛋白或核酸检测。下面以engreen的超敏型ECL发光液Enlight-Plus为例，说一...
生物知识
实验室最常用的移液枪你用对了吗？
移液枪对我们医药生物研究人员来说是最熟悉不过了。可是，你真正了解它吗？你的使用方式是正确的吗？相信不少人听到这个问题后，已经开始回想自己平时的操作，也有一部分人在认...
生物知识
Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz
The green fluorescent protein (GFP) from the Pacific Northwest jellyfishAequorea v...
生物知识
Measurement of Geminin Activity in Xenopus Egg Extracts
Geminin is an unstable protein that inhibits DNA replication by interfering with t...
生物知识
Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens
The process of bringing a new pharmacologically active drug to market is laborious...
生物知识
On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis
This chapter reviews the theory and methodological developments of on-line concent...
生物知识
Scale-Up of Suspension and Anchorage-Dependent Animal Cells
In this chapter, scale-up is described in a laboratory context (10–20 L), but the ...
生物知识
Conventional Micropipette-Based Patch Clamp Techniques
The patch clamp technique revolutionized the study of ion channels and is consider...
