生物知识 An inexpensive alternative to glassmilk for DNA purification Preparation of Silica1. Suspend 5 g of silica (Sigma, S-5631) in 50 ml of PBS.2. A... 05月12日 发表评论 阅读全文
生物知识 Streamlined DNA Extraction Protocol This method is derived from a procedure developed by Toby Bradshaw and the Poplar ... 05月12日 发表评论 阅读全文
生物知识 Preparation of Mouse Tail DNA for Dot Blots or PCR These procedures were originally devised in Richard Palmiter's lab for use wi... 05月12日 发表评论 阅读全文
生物知识 PlasmidDNAminipreps There are many methods for DNA preparation without using kits.Below are a few mini... 05月12日 发表评论 阅读全文
生物知识 BACDNAIsolationwithAutoGen740 1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB wi... 05月12日 发表评论 阅读全文
生物知识 PlasmidMini-prepProtocol 1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on high... 05月12日 发表评论 阅读全文
生物知识 Triton-Prep Method for bacterial DNA Purification 1.Grow 5 ( large scale-15ml culture). Harvest in single eppendorf tube (or 15 ml d... 05月12日 发表评论 阅读全文
生物知识 Phenol-based Method for the Isolatio Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperatur... 05月12日 发表评论 阅读全文
生物知识 DNA重组实验中常用的技术(一) (一)质粒DNA 的提取及鉴定1.收获细菌(1)将2ml含相应抗生素的LB液体培养基加入到通气良好的15ml的试管中,接入一单菌落,于37℃剧烈振荡培养过夜。(2)... 05月12日 发表评论 阅读全文
生物知识 Removal of 32P-ATP from Oligonucleoi 1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate at... 05月12日 发表评论 阅读全文