生物知识 Plasmid This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from... 05月12日 发表评论 阅读全文
生物知识 DNA from Tail Biopsies 1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince).(The tub... 05月12日 发表评论 阅读全文
生物知识 ES CELL DNA EXTRACTION Protocol for extracting DNA from ES Cells,starting from the 96-well plate but proc... 05月12日 发表评论 阅读全文
生物知识 Protocol for Isolation of Genomic DNA from Mammalian Cells Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest an... 05月12日 发表评论 阅读全文
生物知识 Chromosomal DNA Extraction from Gram-positive Bacteria This procedure was originally developed for Listeria monocytogenes but has worked ... 05月12日 发表评论 阅读全文
生物知识 DNA Extraction from Frozen Tissue Sections Tissue collection, storage, microdissection, sectionin g: See separate protocol.Ti... 05月12日 发表评论 阅读全文
生物知识 Southern blotting实验 PURPOSE: TolocateaparticularsequenceofDNAwithinacomplexmixture(locateonegenewithin... 05月12日 发表评论 阅读全文
生物知识 CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA CTAB TECHNIQUE / Method / Schedule / Protocol (JPB) FOR DNA ISOLATION / DNA EXTRAC... 05月12日 发表评论 阅读全文
生物知识 Silica Clean-up of DNA Silica Suspension:add 2 g of silica to 15 ml of H2Owash 3x by centifugation at 200... 05月12日 发表评论 阅读全文
生物知识 Strategy for accurate sequencing through hairpin structures Verifying the sequence of an shRNA hairpin is essentialsince mismatch of even one ... 05月12日 发表评论 阅读全文