Recovery of DNA from LMP (Low Melting Point) Agarose Gel1. Separate DNA fragments ...

Phenol (removes protein)1、add equal volume of Phenol (= tris-saturated Phenol-Chlo...

Glass wool method:Run TAE agarose gel and cut the appropriate band out with a clea...

1、The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB wi...

Preparation of Sonicated Salmon Sperm DNAProcedure:1) Use Pharmacia #27-4564-01. W...

1. Pick single colony and inoculate 250 ml of LB broth containing 100 m g/l ampici...

ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A pe...

Materials:1.TENS solution:10 mM Tris (pH to 7.5)1 mM ethylenediaminetetraacetic ac...

1. Run gel (0.8 -1.0% agarose is best). For yeast chromosomal Southerns, digest 20...

The quality of sequencing results is directlyrelated to the quality of the templat...