Diatomaceous Earth-based Midi-prep Note: This procedure is the method of choice fo...

Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Molecu...

1. Pick single colony and inoculate 5 ml of LB broth containing 200 g/l ampicillin...

After an aliquot of the PCR mixture is analyzed on an agarose gel, the remainder o...

(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Universi...

1、Grow an overnight culture from a single colony in 2 mls LB + antibiotic.2、Transf...

1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sampl...

NH4Ac and EtOH precipitation of DNAAdd NH4Ac (10M stock or solid) to the sample fo...

Procedure for 100 ~ 300 mg of tissue.1. Collect fresh tissue, coleoptiles or small...

Equipment and reagents1. Phenol2. TE buffer,pH 8.0 (10 mM Tris-HCl,pH 8.0;1 mM EDT...