1. Collected animals can be stored as frozen pellets at -70 degrees. Alternatively...
生物知识
生物知识
生物知识
用异硫氰酸胍和有机溶剂提取RNA
收集细胞，离心5000r/min ，5分钟，弃上清，加入异硫氰酸胍变性液（异硫氰酸胍4mol/L；柠檬酸钠25mmol/L，Sarkosy10.5%,β-巯基乙醇0...
生物知识
用异硫氰酸胍和有机溶剂提取ＲＮＡ
收集细胞，离心5000r/min ，5分钟，弃上清，加入异硫氰酸胍变性液（异硫氰酸胍4mol/L；柠檬酸钠25mmol/L，Sarkosy10.5%,β-巯基乙醇0...
生物知识
Decontamination and inhibition of ribonucleases
Decontamination and inhibition of ribonucleasesIt is common knowledge in most labo...
生物知识
Collection of Tissue for RNA Analysis
Handling RNA:Ribonucleases (RNases) are very stable and active enzymes that degrad...
生物知识
Northern Blotting (Howell Lab)
RNA isolationHarvest cells when they are about 80-85% confluent. Stationary phase ...
![[Nature] RNAi研究专题](http://www.bio-review.com/wp-content/themes/begin2_0/img/random/5.jpg)
生物知识
[Nature] RNAi研究专题
Cells utilize RNA interference (RNAi) to regulate protein expression in many conte...
生物知识
Hiro Hirai PRINS Protocol
Hiro Hirai 's PRINS ProtocolOverviewThe PRINS (Primedin situ) technique uses ...
生物知识
E.coli Total RNA Labeling Protocol for Spotted Microarray
Note: Start with 20 mg of total RNA for each labeling reaction. All solutions that...
生物知识
RNAi-制备siRNAs的方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链RN...
