OverviewMost RNA extractions procedures yield RNA, with minimal or no contaminatio...
生物知识
生物知识
生物知识
Preparation of total RNA from whole blood collected in PAXgene tubes
Short PAXgene Protocol:spin in step 6 increased to 20 min; no Dnase treatment; opt...
生物知识
How to isolate mRNA(分离mRNA)
Background: mRNAs （messenger RNAs） comprise only a small percentage of all RNA spe...
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提取棉花RNA–RNA EXTRACTION METHOD FOR COTTON
Based on: Wan, C.-Y. and Wilkins, T.A. 1994. Anal. Bioch. 223:7-12.1.Collect young...
生物知识
TRIzol method for RNA isolation
TRIzol method for RNA isolation-To tissue or cells, add 200- 1 ml TRIzol (GIBCO BR...
生物知识
肝中RNA的分离与鉴定
原理细胞内的核酸多以核蛋白的形式存在，其中脱氧核糖核蛋白主要存在于细胞核中，核糖核蛋白主要存在于细胞质中。这两类核蛋白在0.14mol／L氯化钠溶液中的溶解度相差很...
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啤酒酵母(S. cerevisiae)mRNA的提取
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Colum...
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按大小分离RNA
乙二醛化RNA琼脂糖凝胶电泳和含有甲醛的琼脂糖凝胶电泳均可按大小分离RNA。RNA样品经甲酰胺处理以及经含有甲醛的凝胶电泳分离可能会发生变性，但因为变性RNA比乙二...
生物知识
RNA提取–RNA ISOLATION
DEP treatment solutions. Add 0.3 ml lysing buffer (0.1 M Hepes, 0.2 M EDTA, 10% SD...
生物知识
RNA purification — Trizol
1. Snap freeze ~107 cells or ~100 mg of tissue in liquid nitrogen or dry ice/ethan...
