The Erase-a-Base® System is designed for the rapid construction of plasmid or M13 ...
生物知识
生物知识
生物知识
Restriction Enzyme Buffer
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are ma...
生物知识
DNA 的定量与连接反应
药品试剂：纯化的gusA 片段及pQE31 BamHI/HindIII 片段1% 6-well agarose gelDNA 标准分子量 （λMr, 0.5 μg/...
生物知识
Restriction Enzyme Digestion of DNA
Materials:10X restriction enzyme buffer (see manufacturer's recommendation)DN...
生物知识
Restriction digestion
Restriction enzyme digestions are performed by incubating double-stranded DNA mole...
生物知识
创造新的酶切位点
将互相匹配的DNA末端连接起来可以产生新的酶切位点。这些DNA末端可以通过以下方法获得： 1. 补平5′突出的粘性末端产生平末端 2. 双酶切产生的平末端 3. 双...
生物知识
连接互相匹配的粘性末端，产生新的酶切位点
通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性...
生物知识
DNA rapid CTAB
A Rapid CTAB DNA Isolation TechniqueUseful for RAPD Fingerprinting and Other PCR A...
生物知识
FOOTPRINTING WITH DNASE1
1)probe: same as used for gelshift), isolated by isotacelectrophoresis w/out EtOH ...
生物知识
UV Shadowing
UV shadowing is a technique for visualizing nucleic acids separated on acrylamide/...
