Note: The following protocol is based on using OBI’s vacuum manifold (Product No. ...

Abstract: Single strand conformational polymorphism (SSCP)is the most widely used ...

作分子生物学实验，核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆，通常要作双酶切。为了省时省事儿，我们常常想方设法把两个酶放在一起切。可是事情并非总那么称...

做分子生物学实验，核酸纯化、酶切、克隆算是最基本的步骤了。因为要做定向克隆，通常要作双酶切。为了省时省事儿，我们常常想方设法把两个酶放在一起切。可是事情并非总那么称...

Argonaute - A family of proteins containing multiple domains and involved in RNA i...

CLONE ISOLATION PREP - PROTOCOLBefore you begin isolating DNA from the cultures:--...

Recovery of DNA from Low Melting Point Agarose Gels1.Run digestion products on 0.7...

PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. Lys...

A. Culture Growth:1.For BAC isolation for shotgun library construction:Pick a smea...

Salmon Sperm DNA1.Dissolve 1 g salmon sperm DNA in 100 ml H2 O. 2.Autoclave (20 mi...