mRNA的分离方法较多，其中以寡聚（dT）-纤维素柱层析法最为有效，已成为常规方法。此法利用mRNA 3’末端含有Poly（A+）的特点，在RNA流经寡聚（dT）纤...
生物知识
生物知识
生物知识
Isolation of DNA from Mouse Tai
Isolation of DNA from Mouse Tail Biopsies1. Obtain tail biopsies from 2 to 3 week ...
![CAT ASSAY[TLC METHOD]](http://www.bio-review.com/wp-content/themes/begin2_0/img/random/13.jpg)
生物知识
CAT ASSAY[TLC METHOD]
CAT ASSAY[TLC METHOD]1. Transfer cells to a 15 ml tube.2. Add 5 ml TBS- to flasks,...
生物知识
Alternate Method for Purifying DNA
1.Excise band of interest from a TAE gel; estimate volume by weighing the gel slic...
生物知识
DNA extraction from Mutation Detecti
Source: Contributed by Mohammad Reza Abbaszadegan et al. Abstract: Single strand c...
生物知识
Ethanol Precipitation of DNA
Materials:3 M sodium acetate pH 5.2 or 5 M ammonium acetateDNA100% ethanolMeasure ...
生物知识
Phenol extraction of DNA samples
(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The Universi...
生物知识
FINGERPRINTING PROTOCOL(AGAROSEGEL)
FINGERPRINTING PROTOCOL (AGAROSE GEL) *Restriction digests consist of:15.75 ml ddH...
生物知识
Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydro...
生物知识
DNA Fragment Purification
TE solution10 mM Tris (pH to 7.5)1 mM EDTA (pH to 8.0 to dissolve)2.Frozen agarose...
