简介病毒的预防与治疗根据病毒种类不同而不同，应对症下药。材料与仪器器材：①一次性无菌手套②标本采集管③棉签拭子④PCR仪试剂：①材料：待采标本②PBS缓冲液③琼脂步...
生物知识
生物知识
生物知识
虫媒病毒的血清学检测
简介虫媒病毒（ arbovirus）是指一些通过吸血节肢动物叮咬敏感脊椎动物而引起的自然疫源性疾病及人兽共患病的一群病毒，包括不同基因组的许多病毒家族，如黄病毒科（...
生物知识
DNA 体外重组
简介DNA 体外重组，又称基因克隆，是基因操作中最基本的技术，也是分子生物学的核心技术。DNA 体外重组包括：欲进行重组的 DNA 片段（常称为目的 DNA) 的获...
生物知识
Detection of ERK1/2 Activities Using Affinity Reagents
The three major mammalian mitogen-activated protein kinase (MAPK) subfamilies incl...
生物知识
Affinity-Capture and Solid-Phase Sequencing of Biotinylated Polymerase Chain Reaction Products
The time and labor required for generating nucleotide sequence information has bee...
生物知识
Resin Technologies: Construction and Staining of Resin TMAs
The traditional formaldehyde-fixed paraffin-embedded tissue, and therefore the tis...
生物知识
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
The traditional method for determining the transcription rate of a gene, nuclear r...
生物知识
Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
The transfer or “transfectron” of genetic material into tissue-culture cell lines ...
生物知识
Inserting and Manipulating DNA in a Nanopore with Optical Tweezers
The translocation of small molecules and polymers is an integral process for the f...
生物知识
Genome-Wide Analysis of Membrane Transport Using Yeast Knockout Arrays
The transport of membrane-bound proteins through post-Golgi compartments depends o...
